PT86864A - Molecular cloning and expression of genes encoding proteolytic enzymes - Google Patents

Molecular cloning and expression of genes encoding proteolytic enzymes

Info

Publication number
PT86864A
PT86864A PT86864A PT8686488A PT86864A PT 86864 A PT86864 A PT 86864A PT 86864 A PT86864 A PT 86864A PT 8686488 A PT8686488 A PT 8686488A PT 86864 A PT86864 A PT 86864A
Authority
PT
Portugal
Prior art keywords
bacillus
protoplasts
expression
dna
bacillus cells
Prior art date
Application number
PT86864A
Other languages
English (en)
Other versions
PT86864B (pt
Inventor
Christiaan Albertus Gerardus
Johannes Cornelis Van Der Laan
Leonardus Johannes S Mulleners
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of PT86864A publication Critical patent/PT86864A/pt
Publication of PT86864B publication Critical patent/PT86864B/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
PT86864A 1987-02-27 1988-02-26 Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas PT86864B (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27

Publications (2)

Publication Number Publication Date
PT86864A true PT86864A (pt) 1988-03-01
PT86864B PT86864B (pt) 1992-05-29

Family

ID=8197584

Family Applications (1)

Application Number Title Priority Date Filing Date
PT86864A PT86864B (pt) 1987-02-27 1988-02-26 Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas

Country Status (19)

Country Link
US (1) US5217878A (pt)
JP (1) JP3026567B2 (pt)
KR (1) KR0127560B1 (pt)
CN (1) CN88101681A (pt)
AT (2) ATE180016T1 (pt)
AU (1) AU620026B2 (pt)
BG (1) BG49049A3 (pt)
BR (1) BR8805647A (pt)
CA (1) CA1339100C (pt)
DE (2) DE3856331T2 (pt)
DK (2) DK175738B1 (pt)
FI (3) FI105483B (pt)
HU (1) HU213220B (pt)
LT (1) LT4000B (pt)
LV (1) LV10307B (pt)
NO (1) NO884423D0 (pt)
PT (1) PT86864B (pt)
RU (1) RU2023723C1 (pt)
WO (1) WO1988006624A2 (pt)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0127560B1 (ko) * 1987-02-27 1997-12-29 마가렛트 에이.호른 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
AU622916B2 (en) * 1988-05-05 1992-04-30 Applied Microbiology, Inc Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
CN1530443A (zh) 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ 枯草杆菌酶变异体和组合物
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6420157B1 (en) 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6485957B1 (en) * 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
US6458564B1 (en) * 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
AU2001249811B2 (en) * 2000-04-03 2006-06-08 Maxygen, Inc. Subtilisin variants
EP1495128B1 (en) 2002-03-29 2014-05-07 Genencor International, Inc. Ehanced protein expression in bacillus
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
WO2004101760A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme elip
WO2004101759A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip2
AU2004277913B2 (en) 2003-09-19 2010-04-08 Bakery Technology Centre Bv Preparation of an edible product from dough
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
CN103421760A (zh) * 2003-11-19 2013-12-04 金克克国际有限公司 丝氨酸蛋白酶、编码丝氨酸酶的核酸以及包含它们的载体和宿主细胞
US20090011463A1 (en) * 2004-04-09 2009-01-08 Walter Weyler Pcka Modifications and Enhanced Protein Expression in Bacillus
DK1766002T3 (en) * 2004-06-21 2015-12-07 Novozymes As Stable maintenance of multiple copies of the at least two ORF in the same orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
JP5867984B2 (ja) 2007-03-12 2016-02-24 ダニスコ・ユーエス・インク 修飾プロテアーゼ
DK2460823T3 (da) 2007-05-10 2014-05-05 Danisco Us Inc Modificeret sekretionssystem til øget ekspression af polypeptider ibakterier
WO2009021000A2 (en) 2007-08-06 2009-02-12 University Of Chile Protein and dna sequence encoding a cold adapted subtilisin-like activity
CN101903519B (zh) 2007-12-21 2013-07-31 丹尼斯科美国公司 杆菌中增强的蛋白质生产
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2751266B1 (en) * 2011-09-22 2017-03-29 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
WO2015102814A1 (en) 2013-12-31 2015-07-09 Danisco Us Inc. Enhanced protein expression
US10683528B2 (en) 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
EP3234149A1 (en) 2014-12-19 2017-10-25 Danisco US Inc. Enhanced protein expression
EP3619309B1 (en) * 2017-05-05 2024-04-10 Bioecho Life Sciences GmbH Rapid purification of high quality nucleic acids from biological samples

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (pt) 1967-10-03 1971-06-03
GB1205403A (en) 1967-11-10 1970-09-16 Novo Terapeutisk Labor As Preparation of proteolytic enzyme preparations
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
FR2482980A1 (fr) 1980-05-20 1981-11-27 Biomerieux Sa Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues
JPS6055118B2 (ja) 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
DE3380878D1 (en) * 1982-11-01 1989-12-28 Miles Inc Method of heterologous cloning of gene in bacillus microorganism
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
KR0127560B1 (ko) * 1987-02-27 1997-12-29 마가렛트 에이.호른 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현

Also Published As

Publication number Publication date
US5217878A (en) 1993-06-08
DK200401262A (da) 2004-08-20
KR890700665A (ko) 1989-04-26
LTIP1820A (en) 1995-08-25
FI106726B (fi) 2001-03-30
AU1398088A (en) 1988-09-26
CA1339100C (en) 1997-07-29
FI884904A (fi) 1988-10-24
DK593988A (da) 1988-10-26
FI119028B (fi) 2008-06-30
DE3856331T2 (de) 1999-10-14
JPH01502876A (ja) 1989-10-05
DE3856331D1 (de) 1999-06-17
DK175852B1 (da) 2005-04-04
ATE181110T1 (de) 1999-06-15
ATE180016T1 (de) 1999-05-15
DK593988D0 (da) 1988-10-26
NO884423L (no) 1988-10-05
LT4000B (en) 1996-06-25
CN88101681A (zh) 1988-11-09
FI925413A0 (fi) 1992-11-27
KR0127560B1 (ko) 1997-12-29
BG49049A3 (en) 1991-07-15
PT86864B (pt) 1992-05-29
FI20001152A (fi) 2000-05-15
RU2023723C1 (ru) 1994-11-30
DE3856339T2 (de) 1999-11-04
AU620026B2 (en) 1992-02-13
LV10307A (lv) 1994-10-20
HU213220B (en) 1997-03-28
LV10307B (en) 1995-08-20
FI105483B (fi) 2000-08-31
WO1988006624A3 (en) 1988-11-17
DK175738B1 (da) 2005-02-07
JP3026567B2 (ja) 2000-03-27
BR8805647A (pt) 1989-10-31
FI884904A0 (fi) 1988-10-24
NO884423D0 (no) 1988-10-05
DE3856339D1 (de) 1999-07-15
FI925413A (fi) 1992-11-27
WO1988006624A2 (en) 1988-09-07

Similar Documents

Publication Publication Date Title
PT86864A (pt) Molecular cloning and expression of genes encoding proteolytic enzymes
CN107400677B (zh) 一种基于CRISPR-Cas9系统的地衣芽孢杆菌基因组编辑载体及其制备方法
EP0283075A3 (en) Molecular cloning and expression of genes encoding proteolytic enzymes
Wilms et al. Development of an Escherichia coli whole cell biocatalyst for the production of L-amino acids
IE810297L (en) Cloned amylase
AU2001279674A1 (en) New nucleotide sequences which code for the mdha gene
US5612192A (en) DNA base sequence containing regions involved in the production and secretion of a protein, recombinant DNA including the whole or a part of the DNA base sequence, and method of producing proteins by use of the recombinant DNA
CN113195727A (zh) 用于产生丙烯酸或其盐的方法
DK0500188T3 (da) Fremgangsmåde til fremstilling af alfa-acetomælkesyre
KR950704495A (ko) 카바모일 포스페이트 합성효소ⅱ의 유전정보를 보유하고 있는 뉴클레오티드 배열[nucleotide sequence encoding carbamoyl phosphate synthetase ⅱ]
EP0302838A2 (en) Cloning of the gene coding the isoamylase enzyme and its use in the production of said enzyme
KR100538990B1 (ko) 티벡터와 발현벡터로의 기능을 동시에 가지는 플라스미드및 이를 이용한 목적유전자의 발현
Zograff On the role of the Escherichia coli RNA polymerase sigma factor in T4 phage development
Tsao et al. Cloning and characterization of an α-amylase gene from Streptomyces lividans
WO1986000929A1 (en) Recombinant dna molecule, transformed microorganisms and process for producing penicillin v amidase
DE3586145T2 (de) Rekombinante dns, diese enthaltende mikroorganismen und deren verwendung zur herstellung von amylasen.
JP2005278468A (ja) 光学活性アミノ酸の製造方法
CA2217136A1 (en) Aldehyde oxidase gene derived from plant and utilization thereof
Mermelstein et al. Amplification of homologous fermentative genes in Clostridium acetobutylicum ATCC 824
ES8602933A1 (es) Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion
IT1228924B (it) Clonaggio del gene dell'isoamilasi.
IT1226901B (it) Clonaggio del gene che codifica l'enzima isoamilasi e suo impiego per la produzione di detto enzima o suoi analoghi.

Legal Events

Date Code Title Description
FG3A Patent granted, date of granting

Effective date: 19911127

PC3A Transfer or assignment

Free format text: 970814 GENENCOR INTERNATIONAL, INC. US

MM3A Annulment or lapse

Free format text: LAPSE DUE TO NON-PAYMENT OF FEES

Effective date: 20040531