LT4000B - Expression module, transformed host cell, process for preparing serine protease, method of transformation, dna sequence, plasmid, oligonucleotide - Google Patents

Expression module, transformed host cell, process for preparing serine protease, method of transformation, dna sequence, plasmid, oligonucleotide Download PDF

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Publication number
LT4000B
LT4000B LTIP1820A LTIP1820A LT4000B LT 4000 B LT4000 B LT 4000B LT IP1820 A LTIP1820 A LT IP1820A LT IP1820 A LTIP1820 A LT IP1820A LT 4000 B LT4000 B LT 4000B
Authority
LT
Lithuania
Prior art keywords
bacillus
host cell
dna
serine protease
host
Prior art date
Application number
LTIP1820A
Other languages
English (en)
Lithuanian (lt)
Inventor
Johannes Cornelis Vanderlaan
Leonardus Johannes S Mulleners
Christiaan Albertus Vaneekelen
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of LTIP1820A publication Critical patent/LTIP1820A/xx
Publication of LT4000B publication Critical patent/LT4000B/lt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTIP1820A 1987-02-27 1994-01-28 Expression module, transformed host cell, process for preparing serine protease, method of transformation, dna sequence, plasmid, oligonucleotide LT4000B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27

Publications (2)

Publication Number Publication Date
LTIP1820A LTIP1820A (en) 1995-08-25
LT4000B true LT4000B (en) 1996-06-25

Family

ID=8197584

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP1820A LT4000B (en) 1987-02-27 1994-01-28 Expression module, transformed host cell, process for preparing serine protease, method of transformation, dna sequence, plasmid, oligonucleotide

Country Status (19)

Country Link
US (1) US5217878A (pt)
JP (1) JP3026567B2 (pt)
KR (1) KR0127560B1 (pt)
CN (1) CN88101681A (pt)
AT (2) ATE181110T1 (pt)
AU (1) AU620026B2 (pt)
BG (1) BG49049A3 (pt)
BR (1) BR8805647A (pt)
CA (1) CA1339100C (pt)
DE (2) DE3856339T2 (pt)
DK (2) DK175738B1 (pt)
FI (3) FI105483B (pt)
HU (1) HU213220B (pt)
LT (1) LT4000B (pt)
LV (1) LV10307B (pt)
NO (1) NO884423D0 (pt)
PT (1) PT86864B (pt)
RU (1) RU2023723C1 (pt)
WO (1) WO1988006624A2 (pt)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
WO1989010976A1 (en) * 1988-05-05 1989-11-16 The Public Health Research Institute Of The City O Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) * 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
CA2270180C (en) 1996-11-04 2011-01-11 Novo Nordisk A/S Subtilase variants and compositions
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6485957B1 (en) 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
US6458564B1 (en) 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
AU4981101A (en) * 2000-04-03 2001-10-15 Maxygen Inc Subtilisin variants
EP3023501A1 (en) 2002-03-29 2016-05-25 Danisco US Inc. Enhanced protein expression in bacillus
EP1625208A4 (en) * 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
US20100129862A1 (en) * 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
WO2004101760A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme elip
CA2539423C (en) 2003-09-19 2011-09-06 Ics Holdings, Inc Preparation of an edible product from dough
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
WO2005052161A2 (en) * 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
CN101006174B (zh) * 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
RU2496875C2 (ru) 2007-03-12 2013-10-27 ДАНИСКО ЮЭс ИНК. Мoдифицированные протеазы
CN101679489B (zh) 2007-05-10 2014-05-28 丹尼斯科美国公司 增加细菌内多肽表达的修饰的分泌系统
US8759065B2 (en) 2007-08-06 2014-06-24 University Of Chile Protein and DNA sequence encoding a cold adapted subtilisin-like activity
EP2235176B1 (en) 2007-12-21 2017-06-21 Danisco US Inc. Enhanced protein production in bacillus
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
EP2421973B1 (en) 2009-04-24 2018-05-23 Danisco US Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
JP2014530598A (ja) * 2011-09-22 2014-11-20 ノボザイムスアクティーゼルスカブ プロテアーゼ活性を有するポリペプチドおよびこれをコードするポリヌクレオチド
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
CN106068273B (zh) 2013-12-31 2021-01-08 丹尼斯科美国公司 增强的蛋白质表达
EP3233893B1 (en) 2014-12-16 2021-01-20 Danisco US Inc. Enhanced protein expression
EP3234149A1 (en) 2014-12-19 2017-10-25 Danisco US Inc. Enhanced protein expression
US11578319B2 (en) * 2017-05-05 2023-02-14 Bioecho Life Sciences Gmbh Rapid purification of high quality nucleic acids from biological samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3674643A (en) 1967-11-10 1972-07-04 Novo Terapeutisk Labor As Preparation of proteolytic enzymes having maximum activity at high alkalinity
US3723250A (en) 1967-10-03 1973-03-27 Novo Terapeutisk Labor As Proteolytic enzymes, their production and use
US4480037A (en) 1982-02-08 1984-10-30 Showa Denko Kabushiki Kaisha Alkaline protease and preparation method thereof
US4480043A (en) 1980-05-20 1984-10-30 Biomerieux Process for the preparation of toxoplasmas for the diagnosis of toxoplasmosis, and preparations thus obtained

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
ATE48156T1 (de) * 1982-11-01 1989-12-15 Miles Inc Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus.
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
GR82200B (pt) * 1983-07-06 1984-12-13 Gist Brocades Nv
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
EP0283075B1 (en) * 1987-02-27 1999-05-12 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3723250A (en) 1967-10-03 1973-03-27 Novo Terapeutisk Labor As Proteolytic enzymes, their production and use
US3674643A (en) 1967-11-10 1972-07-04 Novo Terapeutisk Labor As Preparation of proteolytic enzymes having maximum activity at high alkalinity
US4480043A (en) 1980-05-20 1984-10-30 Biomerieux Process for the preparation of toxoplasmas for the diagnosis of toxoplasmosis, and preparations thus obtained
US4480037A (en) 1982-02-08 1984-10-30 Showa Denko Kabushiki Kaisha Alkaline protease and preparation method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHANG S, COHEN SN.: "High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.", MOL GEN GENET., 1979, pages 111 - 115, XP002092527, DOI: doi:10.1007/BF00267940
MANIATIS T, ET AL.: "Molecular cloning"
P. VOROBJEVA ET AL.: "TRANSFORMATION OF BACILLUS MEGATERIUM PROTOPLASTS BY PLASMID DNA", FEMS MICROBIOLOGY LETTERS, 1980, pages 261 - 263, XP001313771
PALVA I. ET AL.: "Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the alpha-amylase gene from Bacillus amyloliquefaciens", GENE, 1981, pages 43 - 51, XP023542084, DOI: doi:10.1016/0378-1119(81)90103-7

Also Published As

Publication number Publication date
LV10307B (en) 1995-08-20
LTIP1820A (en) 1995-08-25
DE3856331T2 (de) 1999-10-14
WO1988006624A3 (en) 1988-11-17
BR8805647A (pt) 1989-10-31
WO1988006624A2 (en) 1988-09-07
DK175738B1 (da) 2005-02-07
AU1398088A (en) 1988-09-26
DE3856339D1 (de) 1999-07-15
BG49049A3 (en) 1991-07-15
CN88101681A (zh) 1988-11-09
FI119028B (fi) 2008-06-30
ATE181110T1 (de) 1999-06-15
FI106726B (fi) 2001-03-30
US5217878A (en) 1993-06-08
KR890700665A (ko) 1989-04-26
PT86864B (pt) 1992-05-29
FI925413A0 (fi) 1992-11-27
DK593988D0 (da) 1988-10-26
PT86864A (pt) 1988-03-01
DK200401262A (da) 2004-08-20
DE3856339T2 (de) 1999-11-04
FI20001152A (fi) 2000-05-15
CA1339100C (en) 1997-07-29
DE3856331D1 (de) 1999-06-17
LV10307A (lv) 1994-10-20
JP3026567B2 (ja) 2000-03-27
FI884904A0 (fi) 1988-10-24
KR0127560B1 (ko) 1997-12-29
NO884423L (no) 1988-10-05
HU213220B (en) 1997-03-28
ATE180016T1 (de) 1999-05-15
NO884423D0 (no) 1988-10-05
FI105483B (fi) 2000-08-31
JPH01502876A (ja) 1989-10-05
FI884904A (fi) 1988-10-24
RU2023723C1 (ru) 1994-11-30
AU620026B2 (en) 1992-02-13
DK593988A (da) 1988-10-26
DK175852B1 (da) 2005-04-04
FI925413A (fi) 1992-11-27

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Free format text: GENENCOR INTERNATIONAL, INC.,4 CAMBRIDGE PLACE, 870 SOUTH WINTON ROAD, ROCHESTER NY 14618,US,970729

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Effective date: 19980128