DK175738B1 - Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer - Google Patents

Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer Download PDF

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Publication number
DK175738B1
DK175738B1 DK198805939A DK593988A DK175738B1 DK 175738 B1 DK175738 B1 DK 175738B1 DK 198805939 A DK198805939 A DK 198805939A DK 593988 A DK593988 A DK 593988A DK 175738 B1 DK175738 B1 DK 175738B1
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DK
Denmark
Prior art keywords
bacillus
host cell
protease
dna
transformed
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DK198805939A
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Danish (da)
English (en)
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DK593988D0 (da
DK593988A (da
Inventor
Leonardus Johannes S Mulleners
Christiaan Albertus Ge Eekelen
Johannes Cornelis Van Der Laan
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Genencor Int
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Publication of DK593988D0 publication Critical patent/DK593988D0/da
Publication of DK593988A publication Critical patent/DK593988A/da
Priority to DK200401262A priority Critical patent/DK175852B1/da
Application granted granted Critical
Publication of DK175738B1 publication Critical patent/DK175738B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK198805939A 1987-02-27 1988-10-26 Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer DK175738B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK200401262A DK175852B1 (da) 1987-02-27 2004-08-20 Transformation af alkalofile Bacillus-stammer

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
EP87200358 1987-02-27
PCT/NL1988/000007 WO1988006624A2 (fr) 1987-02-27 1988-02-26 Clonage et expression moleculaire de genes codant des enzymes proteolytiques
NL8800007 1988-02-26

Publications (3)

Publication Number Publication Date
DK593988D0 DK593988D0 (da) 1988-10-26
DK593988A DK593988A (da) 1988-10-26
DK175738B1 true DK175738B1 (da) 2005-02-07

Family

ID=8197584

Family Applications (2)

Application Number Title Priority Date Filing Date
DK198805939A DK175738B1 (da) 1987-02-27 1988-10-26 Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer
DK200401262A DK175852B1 (da) 1987-02-27 2004-08-20 Transformation af alkalofile Bacillus-stammer

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK200401262A DK175852B1 (da) 1987-02-27 2004-08-20 Transformation af alkalofile Bacillus-stammer

Country Status (19)

Country Link
US (1) US5217878A (fr)
JP (1) JP3026567B2 (fr)
KR (1) KR0127560B1 (fr)
CN (1) CN88101681A (fr)
AT (2) ATE181110T1 (fr)
AU (1) AU620026B2 (fr)
BG (1) BG49049A3 (fr)
BR (1) BR8805647A (fr)
CA (1) CA1339100C (fr)
DE (2) DE3856331T2 (fr)
DK (2) DK175738B1 (fr)
FI (3) FI105483B (fr)
HU (1) HU213220B (fr)
LT (1) LT4000B (fr)
LV (1) LV10307B (fr)
NO (1) NO884423D0 (fr)
PT (1) PT86864B (fr)
RU (1) RU2023723C1 (fr)
WO (1) WO1988006624A2 (fr)

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WO1989010976A1 (fr) * 1988-05-05 1989-11-16 The Public Health Research Institute Of The City O Bacteries gram-positives deficientes en proteases et leur utilisation comme organismes hotes pour la production de produits recombinants
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
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DE69333463D1 (de) * 1992-05-18 2004-05-06 Genencor Int Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
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US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
JP2001503269A (ja) 1996-11-04 2001-03-13 ノボ ノルディスク アクティーゼルスカブ ズブチラーゼ変異体及び、組成物
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6485957B1 (en) 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
US6458564B1 (en) 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
AU2003228393A1 (en) * 2002-03-29 2003-10-13 Genencor International, Inc. Ehanced protein expression in bacillus
US20100129862A1 (en) * 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
WO2004101760A2 (fr) * 2003-05-12 2004-11-25 Genencor International, Inc. Une nouvelle enzyme lipolytique dite elip
US20070213518A1 (en) * 2003-05-12 2007-09-13 Jones Brian E Novel Lipolytic Enzyme Lip2
CA2539423C (fr) 2003-09-19 2011-09-06 Ics Holdings, Inc Preparation d'un produit comestible a base de pate
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
EP1694847B1 (fr) * 2003-11-19 2012-06-13 Danisco US Inc. Serine proteases, acides nucleiques codants pour les enzymes a serine et vecteurs et cellules hotes les contenant
JP2007532114A (ja) * 2004-04-09 2007-11-15 ジェネンコー・インターナショナル・インク バチルスにおけるｐｃｋＡ修飾および増強されたタンパク質発現
WO2005123915A1 (fr) * 2004-06-21 2005-12-29 Novozymes A/S Copies multiples d'au moins deux cadres de lecture ouverte maintenus stables dans la meme orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
KR20090129425A (ko) 2007-03-12 2009-12-16 다니스코 유에스 인크. 개질된 프로테아제
DK2460823T3 (da) 2007-05-10 2014-05-05 Danisco Us Inc Modificeret sekretionssystem til øget ekspression af polypeptider ibakterier
ES2562919T3 (es) 2007-08-06 2016-03-09 University Of Chile Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío
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EP2751266B1 (fr) * 2011-09-22 2017-03-29 Novozymes A/S Polypeptides dotés d'une activité de protéase et polynucléotides codant pour ceux-ci
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Also Published As

Publication number Publication date
BR8805647A (pt) 1989-10-31
CA1339100C (fr) 1997-07-29
KR890700665A (ko) 1989-04-26
FI925413A (fi) 1992-11-27
DE3856331D1 (de) 1999-06-17
AU620026B2 (en) 1992-02-13
FI925413A0 (fi) 1992-11-27
LV10307B (en) 1995-08-20
JP3026567B2 (ja) 2000-03-27
FI119028B (fi) 2008-06-30
WO1988006624A2 (fr) 1988-09-07
LV10307A (lv) 1994-10-20
RU2023723C1 (ru) 1994-11-30
US5217878A (en) 1993-06-08
AU1398088A (en) 1988-09-26
LTIP1820A (en) 1995-08-25
PT86864B (pt) 1992-05-29
WO1988006624A3 (fr) 1988-11-17
NO884423L (no) 1988-10-05
PT86864A (pt) 1988-03-01
FI105483B (fi) 2000-08-31
DK175852B1 (da) 2005-04-04
DE3856339T2 (de) 1999-11-04
KR0127560B1 (ko) 1997-12-29
NO884423D0 (no) 1988-10-05
HU213220B (en) 1997-03-28
BG49049A3 (en) 1991-07-15
FI106726B (fi) 2001-03-30
DE3856331T2 (de) 1999-10-14
DK593988D0 (da) 1988-10-26
DK593988A (da) 1988-10-26
ATE181110T1 (de) 1999-06-15
FI884904A (fi) 1988-10-24
DK200401262A (da) 2004-08-20
CN88101681A (zh) 1988-11-09
FI884904A0 (fi) 1988-10-24
FI20001152A (fi) 2000-05-15
JPH01502876A (ja) 1989-10-05
ATE180016T1 (de) 1999-05-15
DE3856339D1 (de) 1999-07-15
LT4000B (en) 1996-06-25

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