US20030049231A1 - Agent for the anti-adhesion of skin pathogenic flora - Google Patents

Agent for the anti-adhesion of skin pathogenic flora Download PDF

Info

Publication number
US20030049231A1
US20030049231A1 US10/177,589 US17758902A US2003049231A1 US 20030049231 A1 US20030049231 A1 US 20030049231A1 US 17758902 A US17758902 A US 17758902A US 2003049231 A1 US2003049231 A1 US 2003049231A1
Authority
US
United States
Prior art keywords
composition
bacterial agent
bacterium
cfu
adhesion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/177,589
Other languages
English (en)
Inventor
Markus Baur
Ralf Zink
Isabelle Auzanneau
Karine Buffard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nestec SA
Original Assignee
Nestec SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestec SA filed Critical Nestec SA
Assigned to NESTEC S.A. reassignment NESTEC S.A. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BUFFARD, KARINE, AUZANNEAU, ISABELLE, ZINK, RALF, BAUR, MARKUS
Publication of US20030049231A1 publication Critical patent/US20030049231A1/en
Priority to US11/217,930 priority Critical patent/US8685389B2/en
Priority to US14/083,098 priority patent/US9226943B2/en
Priority to US14/957,889 priority patent/US20160082052A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A61K9/0017—Non-human animal skin, e.g. pour-on, spot-on
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/04—Antipruritics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/08—Antiseborrheics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/10—Anti-acne agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/006—Antidandruff preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/02—Preparations for cleaning the hair
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/005—Preparations for sensitive skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/008—Preparations for oily skin

Definitions

  • the present invention relates to the use of a bacterial agent selected for its properties of anti-adhesion of skin pathogens, for the preparation of compositions which are for cosmetic, pharmaceutical or veterinary use and which are intended to stabilize and/or regulate the cutaneous ecosystem of mammals, and to the compositions containing such an agent.
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or of certain yeasts
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or of certain yeasts
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or of certain yeasts
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or of certain yeasts
  • antibiotics or chemical antibacterial agents are known. They are, for example, compositions based on aldehydes and derivatives.
  • French patent application FR 2740039 describes the use of a substance chosen from aldehydes and bifunctional compounds, preferably glutaraldehyde, for inhibiting the attachment of strains of pathogens such as Staphylococcus aureus to keratinocytes and corneocytes.
  • hexachlorophene and its derivatives are known as antibacterial substances and are more particularly used against Propionibacterium acnes.
  • PCT application WO 97/366603 demonstrates the antifungal properties of a strain of Lactobacillus casei.
  • Bacillus can also be used on skin or mucous membranes.
  • Bacillus coagulans Bacillus subtilis, Bacillus laterosporus and Bacillus laevolacticus are used in compositions intended to prevent bacterial, viral or fungal infections of skin or of mucous membranes.
  • the invention proposes to find a novel bacterial agent capable of controlling and regulating the cutaneous ecosystem in order to improve upon the deficiencies of the prior art.
  • the present invention relates to the use of a bacterial agent for preparing a composition which is for cosmetic, pharmaceutical or veterinary use and to the resulting compositions. These compositions are intended to be administered to humans or to animals for the purpose of preventing or treating disorders induced by pathogens of the cutaneous system.
  • the bacterial agent is generally an extract of a lactic acid bacterium, or a lactic acid bacterium, and is selected for its properties of adhesion to skin cells as well as for regulation of the attachment of skin pathogens, in particular by inhibiting their adhesion.
  • Suitable bacterial agents may be selected from strains of Lactobacillus, Micrococcus or Bifidobacterium, and preferably from the Lactobacillus johnsonii CNCM I-1225, Micrococcus varians CNCM I-1586, Micrococcus varians CNCM I- 1587 or Bifidobacterium lactis ATCC 27536 strains.
  • the bacterial strain can be used in a viable, deactivated or semi-active form. It also can be used in the form of a lyophilized powder, which can, e.g., comprise approximately 10 ⁇ 10 8 to 10 ⁇ 10 11 cfu/g.
  • compositions of the present invention are intended for cosmetic, pharmaceutical or veterinary use and contain at least one bacterial agent capable of stabilizing and/or of regulating the pathogenic flora of the cutaneous system.
  • the bacterial agent is an extract of a bacterium, or a bacterium, selected for its properties of adhesion to skin cells and its anti-adhesive properties with respect to pathogens of the cutaneous system.
  • compositions can also be used in ophthalmology or for nasal application. Also, they can in particular be in the form of a cream, lotion, hypoallergenic cleansing bar, shampoo or powder.
  • skin cells i.e. keratinocytes
  • corneocytes are grouped together under the name “cutaneous system”.
  • the present invention provides a bacterial agent selected for its property of adherence to skin cells, and of stabilization and regulation of the pathogenic bacterial flora of the cutaneous system, in particular by inhibiting the adhesion of pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes.
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes.
  • pathogens such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes.
  • strains of Lactobacillus, of Micrococcus and of Bifidobacterium have been found to be useful, with a strain of Lactobacillus johnsonii (NCC 533), two strains of Micrococcus varians (NCC 1482, NCC 1520) and a strain of Bifidobacterium lactis (ATCC 27536) being preferred for selection as the agent.
  • the strain of Bifidobacterium lactis (ATCC 27536) can be obtained from Hansen (Chr. Hansen A/S, 10-12 Boege Alle, P.O. Box 407, DK-2970 Hoersholm, Denmark).
  • CNCM I-1586 NCC 1482
  • CNCM I-1587 NCC 1520
  • Gram-positive microorganism is permanently immobile.
  • Spherical form is in the form of irregularly arranged tetrades.
  • the bacteria according to the invention are used for preparing compositions intended for the prophylaxis or the treatment of disorders linked to pathogens of the cutaneous system, such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or yeasts.
  • disorders linked to pathogens of the cutaneous system such as Staphylococcus aureus, Streptococcus pyogenes or Propionibacterium acnes, or yeasts.
  • These skin disorders can be in particular atopic dermatitis (in the remission phases, as a maintenance treatment), acne, candidiases, seborrhoeic dermatitis, pityriasis versicolor, impetigo or eczematous secondary infections.
  • the disorders of the cutaneous system may also be linked to therapies with antibiotics or antimycotic agents, to diabetes (candidiases), to a pathology of mucous membranes (vaginal candidiasis), to chronic eczema (homeostasis imbalance), to sensitive skin (premature babies, children) or greasy skin (linked to hormonal dysregulation which may promote the growth of bacteria) or to dandruff.
  • therapies with antibiotics or antimycotic agents to diabetes (candidiases), to a pathology of mucous membranes (vaginal candidiasis), to chronic eczema (homeostasis imbalance), to sensitive skin (premature babies, children) or greasy skin (linked to hormonal dysregulation which may promote the growth of bacteria) or to dandruff.
  • the bacteria according to the invention can be used in their live or semi-active form, or in a deactivated form.
  • the expression “bacterium in a semi-active form” is intended to mean a bacterium with low physiological activity. This activity can be measured by a longer exponential growth phase or generation time, a metabolism which has slowed or an incomplete physiological response to modifications of the environment, for example. In certain extreme cases, the number of bacteria may be decreased since they can no longer withstand the change in the environment.
  • Bacterial culture supernatants can also be used successfully in this invention.
  • the bacterial agent can be an extract of a bacterium, or a bacterium itself in a viable active form.
  • the bacterial agent is then preferably converted into a lyophilized powder, for example, according to the method described in EP 818529.
  • the powder typically contains from 10 ⁇ 10 8 to 10 ⁇ 10 11 cfu/g.
  • the bacterial agent can be an extract of a bacterium, or the bacterium itself in a semi-active form.
  • the partial deactivation of the strains can be carried out in several ways, in particular by:
  • freeze drying consisting of cycles of freezing in liquid nitrogen/thawing at 37° C. A decrease of approximately 1 log can then be obtained
  • the bacterial agent can then be used in the form of a powder containing at least 10 ⁇ 10 6 cfu/g, and preferably in dry compositions, such as dry shampoos or other powder compositions, which can contain up to 10% of the bacterial extract.
  • the bacterial agent can also be an extract of a bacterium, or a bacterium, in a deactivated form.
  • the bacterium is preferably inactivated by heat treatment at approximately 90° C. for approximately 2 hours.
  • the bacterial agent is in the form of a lyophilized powder containing from 10 ⁇ 10 8 to 10 ⁇ 10 12 cfu/g. It can be used at up to 5%, and from preferably from 0.05 to 3%, in liquid compositions and at up to 10% in pulverulent compositions.
  • the present invention also relates to a composition which is for cosmetic, pharmaceutical or veterinary use and which contains a bacterial agent having the properties as described above.
  • At least one bacterial strain in viable, semi-active or deactivated form is incorporated into a pharmaceutically or cosmetically acceptable support in an amount which varies as a function of the desired use.
  • the bacterial agent can be present at up to approximately 5% with respect to the total weight of the composition and at up to 10% for compositions in the form of a powder, and preferably at between 0.5 to 2%.
  • compositions according to the invention can be administered via the topical or ocular route.
  • the pharmaceutical compositions based on compounds according to the invention are preferably intended for the treatment of skin and of mucous membranes, and can be in the form of salves, of creams, of milks, of ointments, of powders, of soaked swabs, of solutions, of gels, of sprays, of lotions or of suspensions. They can also be in the form of microspheres or nanospheres, or lipid or polymeric vesicles, or of polymer patches and of hydrogels, which allow controlled release.
  • These compositions for administration via the topical route can be either in anhydrous form or aqueous form, depending on the clinical indication.
  • the invention relates to a cosmetic composition containing, in a cosmetically acceptable support, at least one bacterial agent as defined above.
  • the cosmetic composition can contain the bacterial agent in a proportion of at least 0.001% by weight with respect to the total weight of the composition, and preferably from 0.05 to 3%.
  • This cosmetic composition is in particular intended for body and hair hygiene. It can in particular be in the form of a cream, a milk, a lotion, a gel, microspheres or nanospheres, or lipid or polymeric vesicles, a soap or a shampoo.
  • the viable or inactivated bacterial agent can be combined with retinoids or corticosteroids, or combined with anti-free radicals, with ⁇ -hydroxy or ⁇ -keto acids or their derivatives, or with ion channel blockers.
  • the pharmaceutical and cosmetic compositions according to the invention can also contain inert additives or even pharmacodynamically or cosmetically active additives, or combinations of these additives, and in particular: wetting agents; depigmenting agents such as hydroquinone, azelaic acid, caffeic acid or kojic acid; emollients; moisturizing agents such as glycerol, PEG-400, thiamorpholinone and its derivatives, or urea; anti-seborrhoeic or anti-acne agents, such as S-carboxymethylcysteine, S-benzylcysteamine, their salts and their derivatives, or benzoyl peroxide; antibiotics such as erythromycin and its esters, neomycin, clindamycin and its esters; tetracyclins; antifungal agents such as ketoconazole or 4,5-polymethylene-3-isothiazolinones; agents for promoting the regrowth of
  • composition according to the invention can also contain preserving agents such as para-hydroxybenzoic acid esters, stabilizers, moisture regulators, pH regulators, osmotic pressure modifiers, emulsifying agents, UV-A and UV-B screening agents, and antioxidants such as ⁇ -tocopherol, butylhydroxyanisole or butylhydroxytoluene.
  • preserving agents such as para-hydroxybenzoic acid esters, stabilizers, moisture regulators, pH regulators, osmotic pressure modifiers, emulsifying agents, UV-A and UV-B screening agents, and antioxidants such as ⁇ -tocopherol, butylhydroxyanisole or butylhydroxytoluene.
  • the present invention also relates to a composition which is for veterinary or cosmetic use for animals and which contains at least one bacterial agent as defined above.
  • a composition which is for veterinary or cosmetic use for animals and which contains at least one bacterial agent as defined above.
  • Such a composition can be in the form of dry or liquid shampoos, powders, foams or lotions, for example. It can contain up to 10% of the bacterial agent.
  • composition according to the invention is intended in particular for the therapeutic or prophylactic treatment of healthy, sensitive and/or diseased skin and/or mucous membranes which may exhibit disorders of the cutaneous system, such as in particular:
  • infectious complications such as superinfected atopic dermatitis, impetigo-based eczema, ulcers, wounds, bums, superinfected inflammatory acne,
  • dermatitises such as impetigo, superficial folliculitis,
  • dermatophytoses (Tinea capitis, Tinea corporis, athlete's foot, Hebra's eczema, herpes carcinatus),
  • candidiases vaginal, interdigital, linked to professions at risk or to diabetes
  • compositions for veterinary use are particularly intended to treat or prevent dysfunctions due to staphylococcal infections (due to Staphylococcus aureus, S. intermedians ), streptococcal infections (due to S. pyogenes ) and mycotic infections (candidoses due to C. albicans and pytirosporoses due to P. canis ).
  • the bacterial cultures (1 ml) are incubated in 10 ml of medium (cf. Table 1) overnight.
  • the bacteria are precultured until a concentration of 5.0 ⁇ 10 8 to 10 9 cfu/ml is obtained.
  • the cfu are standardized by measuring the optical density of each strain (OD at 10 8 cfu/ml: see Table 1).
  • the bacterial strains are assayed for their adhesion properties.
  • SV40 T-Ag immortalized cell lines DK2-NR and FK2-NR cells as described in EP 780 469 and,
  • HPV Human papilloma virus
  • E6/E7 and SV40 T-Ag immortalized cell lines DK7-NR cell lines as described in application WO 99/02347.
  • the culture medium for the cell lines was as follows: DK7-NR, FK2-NR: NR-2 (Biofluids, Rockville, Md. 20850) (EP 780469). DK2-NR: NR-M (Biofluids, Rockville, Md. 20850).
  • the keratinocyte lines are cultured in a proportion of 5 ⁇ 10 5 keratinocytes/cm 2 , seeded in coated 6-well clusters (Becton Dickinson, Lincoln Park, N.J.).
  • the coating solution consists of basic medium supplemented with 10 ⁇ g/ml of human fibronectin (Becton Dickinson), 31 ⁇ g/ml of bovine collagen I (Vitrogen, Collagen Corporation, Fremont, Calif.) and 0.1 mg/ml of BSA (Biofluids, Rockville, Md. 20850).
  • the cell cultures form a monolayer (confluent).
  • the Ca 2+ concentration of the medium is brought to 1.5 mM so as to induce cell differentiation.
  • the cells are cultured for 4 days in a high calcium concentration medium, without antibiotics.
  • the adhesion assays the cell cultures are washed 3 times with buffer (HBSS, Ca 2+ : 1.0 mM).
  • the bacterial strains are labelled overnight by adding 100 ⁇ Ci/10 ml of 2- 3 H-adenine (Amersham, TRK.311) to the culture medium. Aliquot fractions of the bacteria are incubated in a medium without 3 H-adenine. The unlabelled (cold) supernatant is set aside in order to adjust the cfu/ml for the adhesion assays.
  • the bacterial suspensions are centrifuged for 10 minutes are 4000 rpm. Before adjusting the optical density (OD), the pellets are washed twice in HBSS. The OD is measured for each strain so as to adjust the final concentrations of bacteria to 10 6 , 10 7 , 10 8 and 10 9 cfu/ml.
  • the medium for the adhesion assays is a 1:1 mixture of keratinocyte culture medium and of the unlabelled supernatant of the bacterial medium.
  • the bacterial suspensions are incubated on plastic dishes and plastic dishes coated with cells.
  • the adhesion index (AI) is calculated as 3 H activity (dpm/well), as % of the total 3 H activity (dpm/ml) of the bacterial suspension.
  • the adhesion index of the 13 different bacterial strains is calculated by measuring the 3 H adenine activity of the radiolabelled microorganisms. The results are given in Table 2.
  • Immortalized human keratinocytes of the HaCaT line are used (Boukamp P. et al., J. Cell Biol., 106, 761-771, 1988).
  • the HaCaT cells are cultured in DMEM medium supplemented with 10% of foetal calf serum, at 37° C. under 5% of CO 2 .
  • 6-well clusters (Becton Dickinson) are seeded in a proportion of 10 4 cells/cm 2 . After 4 to 5 days, the cells reach confluence. The adhesion assays are carried out 4 to 5 days after confluence. The monolayers are washed 3 times with PBS before these assays.
  • the bacteria are labelled with 2- 3 H-adenine (Amersham, TRK 311), in a proportion of 100 ⁇ Ci/10 ml of broth.
  • the suspensions are washed 3 times and then resuspended in PBS.
  • the cell density is adjusted in this same buffer.
  • the adhesion is defined by the ratio between radioactivity which has adhered and radioactivity which was introduced, multiplied by 100.
  • the radiolabelled pathogen and the cold bacterial strain are incubated simultaneously with the monolayer.
  • the assays are carried out in triplicate for bacterial agent densities covering 3 logs.
  • the in vitro adhesion model is based on the incubation of a radiolabelled and calibrated suspension of a skin pathogenic microorganism ( Staphylococcus aureus ) with a monolayer of immortalized human keratinocytes (HaCaT line) (Boukamp P. et al., J. Cell Biol., 106, 761-771, 1988).
  • a skin pathogenic microorganism Staphylococcus aureus
  • HaCaT line monolayer of immortalized human keratinocytes
  • the inhibitory activity of the bacterial agent ( Lactobacillus johnsonii NCC 533 in a viable or deactivated form) with respect to this adhesion is evaluated in the context of a co-incubation, on the monolayer, of the pathogen and of the compound to be assayed, by measuring the radioactivity retained on the monolayer.
  • the HaCaT cells are cultured in DMEM supplemented with 10% of foetal calf serum, at 37° C. under 5% of CO 2 . They are seeded in 6-well clusters in a proportion of 10 4 cells/cm 2 .
  • the adhesion assay is carried out 5 days after confluence. The monolayers are washed 3 times with PBS before incubation with the microorganisms.
  • Staphylococcus aureus (ATCC 6538) is cultured in TCS medium, in aerobiosis at 35° C.
  • Lactobacillus johnsonii NCC 533 is cultured in MRS medium, in anaerobiosis at 37° C.
  • the deactivated form of NCC 533 is obtained by lyophilizing a dense suspension of Lactobacilli which has been subjected to several cycles of freezing in liquid nitrogen/thawing at room temperature.
  • the preparation assayed corresponds to a biomass of 4.0 ⁇ 10 10 cfu/g.
  • mice 15 7- to 8-week-old SKH female mice weighing approximately 30 g were supplied by C. River. 5 mice were used for each group assaying a different topical application.
  • Microorganisms A strain of Staphylococcus aureus (named: strain 1) which was isolated from a human skin lesion (leg ulcer) is used. This strain is sensitive to methycilin.
  • a suspension of the bacterium is prepared for inoculation in the mice.
  • a preculture in the exponential growth phase of strain 1 is prepared on a solid medium (AES, AEB 122 859) at 35° C. for 18 to 24 h.
  • the bacterium is resuspended in 10 ml of sterile saline solution, and then recovered after centrifugation at 3000 [lacuna] for 10 min. The supernatant is then removed and the pellet is taken up with 10 ml of saline solution. This procedure is repeated twice.
  • An inoculum suspension is prepared by resuspending the washed bacteria in 4 ml of sterile saline solution. The OD at 525 nm is adjusted to approximately 0.14. It contains approximately 10 8 cfu/ml.
  • mice The skin of the mice is delipidized on the flanks with 95 [sic] ethanol (Merck). 50 ⁇ l of a suspension containing a 50/50 mixture of the S. aureus inoculum, 10 7 cfu/ml, and of the product to be assayed were slowly applied to the delipidized area (6.25 cm 2 ), using a micropipette. The inoculated sites are protected by occlusion for 1 h under a sterile plastic dressing (Dermafilm 33 ⁇ 15, ref. 38.3015, Vygon laboratory).
  • mice 4 hours after application of the suspension, the mice are killed under anaesthesia with forene (Abbott France).
  • the inoculated sites are excised as a block (12 mm diameter).
  • the skin biopsies removed are ground and homogenized with 2 ml of sterile saline solution, using a Polytron (PT 2100, Bioblock Scientific) (5 rpm, 5 min.).
  • a 1 ml sample of the homogenized tissue is added to 9 ml of a sterile saline solution, and 0.1 ml of this mixture is cultured on a staphylococcal medium No. 110 using the 10-fold dilutron method. After 48 hours of incubation at 35° C., the colonies developed are counted and the CFU(colony forming units) are determined.
  • NCC 533 at 0.5% and 1% decreases the number of S. aureus bacteria found by approximately 1 log for the inocula at 10 6 cfu/ml and 10 7 cfu/ml. No dose effect is observed, either with the 10 6 cfu/ml inoculum or with the inoculum at 10 7 cfu/ml.
  • a body lotion is prepared which has the following composition: 8.0% of mineral oil, 5.0% of isopropyl palmitate, 2.0% of polyglyceryl-3 diisostearate, 4.0% of octyldodecanol, 0.3% of carbomer, 0.2% of sodium cocoylglutamate, 1.2% of 10% sodium hydroxide, a preserving agent, fragrance, 0.5 to 3% of a lyophylisate containing from 10 ⁇ 10 8 to 10 ⁇ 10 12 cfu/g of at least one bacterial strain chosen from Lactobacillus johnsonii (CNCM I-1225), Micrococcus varians (CNCM I-1586 or CNCM I-1587) or Bifidobacterium lactis (ATCC 27536, Hansen) and inactivated by heat treatment at approximately 90° C. for about 2 hours. The mixture is made up to 100% with water.
  • the body lotion thus obtained is intended, due to its anti-adhesion properties with respect to pathogens, to stabilize and/or regulate skin pathogenic flora.
  • a shampoo is prepared which has the following composition: 7.0% of sodium lauryl sulphate, 2.0% of cocamidopropyl betaine, 2.0% of sodium lauryl sulphonosuccinate, sodium chloride, preserving agent, fragrance and from 0.5 to 3% of a lyophilisate containing from 10 8 to 10 12 cfu/g of at least one bacterial strain chosen from Lactobacillus johnsonii (CNCM I-1225), Micrococcus varians (CNCM I-1586 or CNCM I-1587) or Bifidobacterium lactis ATCC 27536, and inactivated by heat treatment at approximately 90° C. for about 2 hours. The mixture is made up to 100% with water.
  • the shampoo thus prepared has properties which regulate scalp pathogenic flora. It is in particular indicated in the treatment of dandruff.
  • fatty and aqueous phases are prepared which have the following composition: L. johnsonii (CNCM I-1225) 1% as described in Example 5
  • Fatty phase Arachidyl behenyl alcohol/ 3% arachidylglucoside Isohexadecane 7% Sweet almond oil 3% Karite butter 2% B.H.T. 0.05%
  • Aqueous phase Water Qs 100% Glycerol 5% Methyl POB 0.1%
  • the fatty and aqueous phases are heated to 75° C. Then, emulsification is carried out by adding the aqueous phase to the fatty phase with Rayneri mixing at 1000 rpm. 30 minutes after the emulsification, the mixture is homogenized for 1 minute with a polytron (speed 4-5).
  • Example 7 In the same way as in Example 7, a composition is prepared which has the following composition: L. johnsonii (CNCM I-1225) 1% as described in Example 5 Fatty phase: Glyceryl stearate and 5% PEG100 stearate Isohexadecane 8% Karite butter 5% B.H.T. 0.05% DC 1503 1% Aqueous phase: Water Qs 100% Glycerol 3% Carbopol 981 0.2% Lubrajel 5% Phenoxyethanol 1% Sodium hydroxide Qs pH 6
  • Example 7 In the same way as in Example 7, a composition is prepared which has the following composition: L. johnsonii (CNCM I-1225) 1% as described in Example 5 Fatty phase: Polyglyceryl-3 diisostearate 5% Cyclomethicone CM5 20% Aqueous phase: Water Qs 100% Glycerol 5% NaCl 0.5% MgSO4 0.5%
  • a shampoo for pets is prepared which has the following composition: 5% of sodium lauryl sulphate, 2% of cocamidopropyl betaine, 2% of sodium lauryl sulphonosuccinate, 2% of sodium chloride, 1.5% of PEG-7 glyceryl cocoate, 0.75% of propylene glycol, panthenol, glycerol, disodium phosphate, preserving agent, fragrance and 1% of L. johnsonii (CNCM I-1225) as described in Example 5. The mixture is made up to 100% with water.
  • the shampoo thus prepared has properties which regulate the pathogenic flora of the cutaneous system of pets.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Pulmonology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
US10/177,589 1999-12-22 2002-06-21 Agent for the anti-adhesion of skin pathogenic flora Abandoned US20030049231A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/217,930 US8685389B2 (en) 1999-12-22 2005-09-01 Agent for the anti-adhesion of skin pathogenic flora
US14/083,098 US9226943B2 (en) 1999-12-22 2013-11-18 Lactobacillus johnsonii CNCM I-1225 for the anti-adhesion of skin pathogenic flora
US14/957,889 US20160082052A1 (en) 1999-12-22 2015-12-03 Agent for the anti-adhesion of skin pathogenic flora

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99204489A EP1110555A1 (fr) 1999-12-22 1999-12-22 Agent anti-adhesion de la flore pathogene de la peau
EP99204489.1 1999-12-22
PCT/EP2000/012719 WO2001045721A1 (fr) 1999-12-22 2000-12-13 Agent anti-adhesion de la flore pathogene de la peau

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012719 Continuation WO2001045721A1 (fr) 1999-12-22 2000-12-13 Agent anti-adhesion de la flore pathogene de la peau

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/217,930 Division US8685389B2 (en) 1999-12-22 2005-09-01 Agent for the anti-adhesion of skin pathogenic flora

Publications (1)

Publication Number Publication Date
US20030049231A1 true US20030049231A1 (en) 2003-03-13

Family

ID=8241061

Family Applications (4)

Application Number Title Priority Date Filing Date
US10/177,589 Abandoned US20030049231A1 (en) 1999-12-22 2002-06-21 Agent for the anti-adhesion of skin pathogenic flora
US11/217,930 Expired - Fee Related US8685389B2 (en) 1999-12-22 2005-09-01 Agent for the anti-adhesion of skin pathogenic flora
US14/083,098 Expired - Fee Related US9226943B2 (en) 1999-12-22 2013-11-18 Lactobacillus johnsonii CNCM I-1225 for the anti-adhesion of skin pathogenic flora
US14/957,889 Abandoned US20160082052A1 (en) 1999-12-22 2015-12-03 Agent for the anti-adhesion of skin pathogenic flora

Family Applications After (3)

Application Number Title Priority Date Filing Date
US11/217,930 Expired - Fee Related US8685389B2 (en) 1999-12-22 2005-09-01 Agent for the anti-adhesion of skin pathogenic flora
US14/083,098 Expired - Fee Related US9226943B2 (en) 1999-12-22 2013-11-18 Lactobacillus johnsonii CNCM I-1225 for the anti-adhesion of skin pathogenic flora
US14/957,889 Abandoned US20160082052A1 (en) 1999-12-22 2015-12-03 Agent for the anti-adhesion of skin pathogenic flora

Country Status (20)

Country Link
US (4) US20030049231A1 (no)
EP (2) EP1110555A1 (no)
JP (1) JP4828759B2 (no)
KR (1) KR100797819B1 (no)
CN (2) CN1434716B (no)
AR (1) AR030925A1 (no)
AT (1) ATE471152T1 (no)
AU (1) AU3011801A (no)
BR (1) BR0016709A (no)
CA (1) CA2395419C (no)
CY (1) CY1111029T1 (no)
DE (1) DE60044566D1 (no)
DK (1) DK1244463T3 (no)
ES (1) ES2345761T3 (no)
MX (1) MXPA02006222A (no)
NO (1) NO20022989L (no)
PL (1) PL355411A1 (no)
PT (1) PT1244463E (no)
RU (1) RU2002119396A (no)
WO (1) WO2001045721A1 (no)

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004023612A1 (de) * 2004-05-10 2005-12-01 Helmut Meusel Verwendung von microbiellen Mischbiozönosen zur Behandlung von Entzündungen der Haut
WO2006013441A2 (en) * 2004-07-29 2006-02-09 Proge Farm S.R.L. Use of probiotic bacteria for the preparation of topical compositions for skin protection
FR2879452A1 (fr) * 2004-12-21 2006-06-23 Oreal Utilisation d'un extrait de bacterie filamenteuse non photosynthetique non fructifiante en tant qu'agent modulant l'adhesion de microorganismes cutanes
US20060171936A1 (en) * 2004-10-04 2006-08-03 L'oreal Cosmetic and/or dermatological composition for sensitive skin
WO2007039086A1 (en) * 2005-09-19 2007-04-12 Basf Aktiengesellschaft Microorganisms inhibiting the formation of foot malodor
EP1987860A2 (fr) 2007-04-27 2008-11-05 L'oreal Film hydrosoluble cosmétique
US20090068161A1 (en) * 2007-09-04 2009-03-12 L'oreal Use of a combination of hesperidin and of a microorganism for influencing the barrier function of the skin
US20090068160A1 (en) * 2007-09-04 2009-03-12 L'oreal Use of a lysate of bifidobacterium species for treating sensitive skin
US20090098053A1 (en) * 2002-04-09 2009-04-16 Nestec S.A. La1 - the genome of a lactobacillus strain
US20090232785A1 (en) * 2005-08-01 2009-09-17 L'oreal Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin
US20100055082A1 (en) * 2008-09-04 2010-03-04 Jacques Alain Bauer Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
EP2161329A1 (en) * 2008-09-04 2010-03-10 OM Pharma Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
US20100129324A1 (en) * 2006-11-06 2010-05-27 Vanessa Crespy biological effects of compositions comprising rosmarinic acid
US20100226892A1 (en) * 2009-03-04 2010-09-09 L'oreal Use of probiotic microorganisms to limit skin irritation
US20100278793A1 (en) * 2008-10-28 2010-11-04 L'oreal Treatment of greasy skin with a bacterial lystate
US20100305053A1 (en) * 2007-08-02 2010-12-02 L'oreal Use of hesperidin or of a derivative thereof for the prevention and/or treatment of slackened skin
US20110182861A1 (en) * 2008-07-29 2011-07-28 Isabelle Castiel Cosmetic use of microorganisms for the treatment of oily skin
US20140065209A1 (en) * 2011-05-03 2014-03-06 Dupont Nutrition Biosciences Aps Probiotic bacteria for the topical treatment of skin disorders
AU2010247469B2 (en) * 2009-05-11 2014-12-18 Société des Produits Nestlé S.A. Lactobacillus johnsonii La1 NCC533 (CNCM I-1225) and immune disorders
US20150093462A1 (en) * 2013-09-30 2015-04-02 Elc Management Llc Watery Lotion Skin Care Compositions And Methods
WO2016176380A1 (en) * 2015-04-28 2016-11-03 The Procter & Gamble Company Compositions comprising whole, non-viable micrococcus for improving skin health
US20180140540A1 (en) * 2016-11-23 2018-05-24 Gojo Industries, Inc. Topical cleansing composition with prebiotic/probiotic additive
WO2019226002A1 (ko) * 2018-05-23 2019-11-28 주식회사 고바이오랩 락토바실러스 크리스파투스 kbl693 균주 및 그 용도
US11154731B2 (en) 2007-09-04 2021-10-26 L'oreal Cosmetic use of Bifidobacterium species lysate for the treatment of dryness

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0124580D0 (en) * 2001-10-12 2001-12-05 Univ Reading New composition
PT1478329E (pt) * 2002-02-21 2006-09-29 Nestle Sa Composicao administravel por via oral para fotoproteccao da pele
FR2844198A1 (fr) * 2002-09-06 2004-03-12 Oreal Utilisation d'un activeur de protectin pour ameliorer la resistance de la peau, composition contenant de tels activateurs et procede de selection
AU2005257033B2 (en) * 2004-06-23 2011-05-12 L'oreal Method and compositions useful for preventing and/or treating sensitive and/or dry skin
FR2872047B1 (fr) * 2004-06-23 2007-06-15 Oreal Composition pour peaux sensibles associant cation mineral et probiotique(s)
EP1642570A1 (fr) 2004-10-04 2006-04-05 L'oreal Composition cosmétique et/ou dermatologique pour peaux sensibles
EP1731137A1 (fr) * 2005-06-08 2006-12-13 Nestec S.A. Composition cosmétique ou dermatologique pour peaux sèches et/ou sensibles
JP2005139198A (ja) * 2004-12-11 2005-06-02 Koichi Takezaki 抑臭効果を付加した生活関連商品
JP4726109B2 (ja) * 2005-02-10 2011-07-20 昭和電工株式会社 皮膚外用剤、それを用いる皮膚有害微生物の付着予防方法及び増殖防止方法
FR2930443B1 (fr) 2008-04-29 2010-06-25 Oreal Produit extemporane de soin a base d'un lyophilisat de microorganisme et de tensioactif(s) de hlb superieur ou egal a 12
FR2938429B1 (fr) * 2008-11-19 2012-05-18 Oreal Utilisation cosmetique de microorganisme(s) pour le traitement des desordres du cuir chevelu
BRPI0916690B1 (pt) * 2008-07-29 2022-04-12 L'oreal Uso de uma quantidade eficaz de pelo menos um microorganismo probiótico, composição coméstica e/ou dermatólogica, método coméstico para tratar e/ou prevenir distúrbios estéticos do couro cabeludo em um indivíduo, processo cosmético para prevenir e/ou tratar uma condição de caspa do couro cabeludo, conjunto cosmético, e, uso cosmético de pelo menos uma quantidade eficaz de pelo menos um primeiro e um segundo agente cosmético ativo
FR2945944B1 (fr) * 2009-06-02 2011-07-15 Oreal Procede cosmetique de traitement des etats pelliculaires comprenant l'administration d'un microorganisme probiotique et d'un actif antipelliculaire
FR2937536B1 (fr) * 2008-10-28 2016-07-01 Oreal Utilisation cosmetique d'un lysat de bifidobacterium species pour le traitement du cuir chevelu gras
EP2251022A1 (en) * 2009-05-11 2010-11-17 Nestec S.A. Non-replicating micro-organisms and their immune boosting effect
EP2251020A1 (en) * 2009-05-11 2010-11-17 Nestec S.A. Short-time high temperature treatment generates microbial preparations with anti-inflammatory profiles
GB2472790A (en) * 2009-08-18 2011-02-23 Laurence Christian Hayes Dermatological preparations comprising microbes.
EP2364712B1 (en) * 2010-03-11 2013-03-06 Kloarys Investment Ltd Topical cosmetic or pharmaceutical composition comprising probiotic lactobacillus strains and use of same
FR2959128B1 (fr) * 2010-04-23 2012-07-13 Oreal Utilisation cosmetique d'un lysat de bifidobacterium species pour le traitement des odeurs corporelles
DE102011009798B4 (de) * 2011-01-31 2015-03-05 Merz Pharma Gmbh & Co. Kgaa Balneologische lipidhaltige probiotische Zubereitungen für kosmetische / dermatologische / medizinische Anwendungen
US10111913B2 (en) 2011-02-04 2018-10-30 Joseph E. Kovarik Method of reducing the likelihood of skin cancer in an individual human being
US11844720B2 (en) 2011-02-04 2023-12-19 Seed Health, Inc. Method and system to reduce the likelihood of dental caries and halitosis
US11951140B2 (en) 2011-02-04 2024-04-09 Seed Health, Inc. Modulation of an individual's gut microbiome to address osteoporosis and bone disease
US10245288B2 (en) 2011-02-04 2019-04-02 Joseph E. Kovarik Method and system for reducing the likelihood of developing NASH in an individual diagnosed with non-alcoholic fatty liver disease
US10842834B2 (en) 2016-01-06 2020-11-24 Joseph E. Kovarik Method and system for reducing the likelihood of developing liver cancer in an individual diagnosed with non-alcoholic fatty liver disease
US11357722B2 (en) 2011-02-04 2022-06-14 Seed Health, Inc. Method and system for preventing sore throat in humans
US11419903B2 (en) 2015-11-30 2022-08-23 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US11191665B2 (en) 2011-02-04 2021-12-07 Joseph E. Kovarik Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being
US10940169B2 (en) 2015-11-30 2021-03-09 Joseph E. Kovarik Method for reducing the likelihood of developing cancer in an individual human being
US10687975B2 (en) 2011-02-04 2020-06-23 Joseph E. Kovarik Method and system to facilitate the growth of desired bacteria in a human's mouth
US10010568B2 (en) 2011-02-04 2018-07-03 Katherine Rose Kovarik Method and system for reducing the likelihood of a spirochetes infection in a human being
US11951139B2 (en) 2015-11-30 2024-04-09 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US10085938B2 (en) 2011-02-04 2018-10-02 Joseph E. Kovarik Method and system for preventing sore throat in humans
US11273187B2 (en) 2015-11-30 2022-03-15 Joseph E. Kovarik Method and system for reducing the likelihood of developing depression in an individual
US10835560B2 (en) 2013-12-20 2020-11-17 Joseph E. Kovarik Reducing the likelihood of skin cancer in an individual human being
US11523934B2 (en) 2011-02-04 2022-12-13 Seed Health, Inc. Method and system to facilitate the growth of desired bacteria in a human's mouth
US10512661B2 (en) 2011-02-04 2019-12-24 Joseph E. Kovarik Method and system for reducing the likelihood of developing liver cancer in an individual diagnosed with non-alcoholic fatty liver disease
US10086018B2 (en) 2011-02-04 2018-10-02 Joseph E. Kovarik Method and system for reducing the likelihood of colorectal cancer in a human being
US9987224B2 (en) 2011-02-04 2018-06-05 Joseph E. Kovarik Method and system for preventing migraine headaches, cluster headaches and dizziness
US10548761B2 (en) 2011-02-04 2020-02-04 Joseph E. Kovarik Method and system for reducing the likelihood of colorectal cancer in a human being
US10583033B2 (en) 2011-02-04 2020-03-10 Katherine Rose Kovarik Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being
CA2831345C (en) * 2011-05-06 2022-05-03 Organobalance Medical Ag Lactic acid bacterial strains that coaggregate with staphylococcus aureus, and compositions and uses of the strains
FR2998174B1 (fr) * 2012-11-21 2016-06-10 Greentech Procede de preparation d'un principe actif cosmetique ou dermatologique
FR2999932B1 (fr) 2012-12-21 2020-01-31 Société des Produits Nestlé S.A. Utilisation de microorganismes probiotiques comme agent favorisant la synthese de melanine
FR2999931A1 (fr) 2012-12-21 2014-06-27 Oreal Utilisation de microorganismes probiotiques comme agent favorisant la synthese de melanine
US11969445B2 (en) 2013-12-20 2024-04-30 Seed Health, Inc. Probiotic composition and method for controlling excess weight, obesity, NAFLD and NASH
US11839632B2 (en) 2013-12-20 2023-12-12 Seed Health, Inc. Topical application of CRISPR-modified bacteria to treat acne vulgaris
US11826388B2 (en) 2013-12-20 2023-11-28 Seed Health, Inc. Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation
US11642382B2 (en) 2013-12-20 2023-05-09 Seed Health, Inc. Method for treating an individual suffering from bladder cancer
US11529379B2 (en) 2013-12-20 2022-12-20 Seed Health, Inc. Method and system for reducing the likelihood of developing colorectal cancer in an individual human being
US11026982B2 (en) 2015-11-30 2021-06-08 Joseph E. Kovarik Method for reducing the likelihood of developing bladder or colorectal cancer in an individual human being
US11980643B2 (en) 2013-12-20 2024-05-14 Seed Health, Inc. Method and system to modify an individual's gut-brain axis to provide neurocognitive protection
US11833177B2 (en) 2013-12-20 2023-12-05 Seed Health, Inc. Probiotic to enhance an individual's skin microbiome
US11672835B2 (en) 2013-12-20 2023-06-13 Seed Health, Inc. Method for treating individuals having cancer and who are receiving cancer immunotherapy
US11213552B2 (en) 2015-11-30 2022-01-04 Joseph E. Kovarik Method for treating an individual suffering from a chronic infectious disease and cancer
JP2017521059A (ja) 2014-06-17 2017-08-03 ザイクローブ セラピューティクス, インク.Xycrobe Therapeutics, Inc. 機械的に位置合わせされた光学的要素及びその製造方法
KR20180056775A (ko) * 2015-10-07 2018-05-29 갈데르마 리써어치 앤드 디벨로프먼트 유산균 함유 조성물 및 아토피 피부염을 치료하기 위한 이의 용도
US10419395B2 (en) * 2015-10-23 2019-09-17 International Business Machines Corporation Routing packets in a data center network
US10933128B2 (en) 2015-11-30 2021-03-02 Joseph E. Kovarik Method and system for protecting honey bees from pesticides
US10568916B2 (en) 2015-11-30 2020-02-25 Joseph E. Kovarik Method and system for protecting honey bees, bats and butterflies from neonicotinoid pesticides
US11529412B2 (en) 2015-11-30 2022-12-20 Seed Health, Inc. Method and system for protecting honey bees from pesticides
US10086024B2 (en) 2015-11-30 2018-10-02 Joseph E. Kovarik Method and system for protecting honey bees, bats and butterflies from neonicotinoid pesticides
US10675347B2 (en) 2015-11-30 2020-06-09 Joseph E. Kovarik Method and system for protecting honey bees from fipronil pesticides
US11504404B2 (en) 2016-02-24 2022-11-22 Crown Laboratories, Inc. Skin probiotic formulation
RU2652277C1 (ru) * 2017-04-13 2018-04-25 Артем Михайлович Гурьев Микрокапсулы, содержащие живые микроорганизмы, и их применение
WO2018216744A1 (ja) * 2017-05-23 2018-11-29 一丸ファルコス株式会社 乳酸菌を含有する化粧料、皮膚保護剤
EP3666341B1 (en) * 2018-12-14 2022-03-23 DWI - Leibniz-Institut für Interaktive Materialien e.V. Topical formulation in form of a patch, a bandage or a plaster comprising probiotic bacteria, and use thereof in a method for treating or preventing skin disorders
US20200368146A1 (en) * 2019-05-22 2020-11-26 Mary Ahern Method of cosmetic preservation
CN111534447B (zh) * 2019-12-11 2022-05-24 沈阳博阳饲料股份有限公司 约氏乳杆菌及其应用
FR3124077A1 (fr) * 2021-06-22 2022-12-23 Nanyang Technological University Utilisation d ’ un acide gras à cha î ne courte comme agent antipelliculaire

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324515A (en) * 1991-10-22 1994-06-28 Pacific Corporation Cosmetic material obtained from a lactic acid fermentation broth
US5422370A (en) * 1986-12-23 1995-06-06 Tristrata Inc Method of using 2-hydroxypropanoic acid (lactic acid) for the treatment of wrinkles
US5578302A (en) * 1992-07-06 1996-11-26 Nestec S.A. Treatment of stomach ulcers
US5603930A (en) * 1992-07-06 1997-02-18 Nestec S.A. Lactobacillus johnsonii CNCM I-1225
US5614209A (en) * 1993-12-03 1997-03-25 Lafor Laboratories Limited Micro-encapsulated lactobacilli for medical applications
US5981261A (en) * 1995-08-07 1999-11-09 Nestec, S.A. Micrococcus varians strains for producing bactericide compositions
US6270811B1 (en) * 1996-11-26 2001-08-07 1 Fois 1 Jour Concept Pharmaceutical, cosmetic composition with base of microbial culture mixed with an essential oil and an acid
US6645506B1 (en) * 1997-04-18 2003-11-11 Ganeden Biotech, Inc. Topical compositions containing extracellular products of Pseudomonas lindbergii and Emu oil

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3024318A1 (de) * 1980-06-27 1982-01-28 Chemisches Laboratorium Dr. Karl Richter GmbH, 1000 Berlin Kosmetische mittel
JPS5920220A (ja) * 1982-07-23 1984-02-01 Yakult Honsha Co Ltd 損傷皮膚創面治療剤
JPH0818995B2 (ja) * 1987-01-21 1996-02-28 株式会社アドバンス 皮膚用抗菌剤
JPH0517363A (ja) * 1991-07-11 1993-01-26 Yakult Honsha Co Ltd 抗炎症剤及びそれを含有する化粧料
CN1110147A (zh) * 1994-04-09 1995-10-18 山田武敏 抗变态反应组合物
WO1995035389A1 (en) * 1994-06-17 1995-12-28 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Method for introduction of genetic material into microorganisms and transformants obtained therewith
US5656268A (en) * 1995-04-21 1997-08-12 Sorodsky; Michael Biological product
JPH092959A (ja) * 1995-06-16 1997-01-07 Yakult Honsha Co Ltd ＩｇＥ抗体産生抑制剤および抗アレルギー剤
JPH09263539A (ja) * 1996-03-29 1997-10-07 Nichinichi Seiyaku Kk 抗真菌作用を有する皮膚疾患治療剤
AUPN907296A0 (en) * 1996-04-02 1996-04-26 Commonwealth Scientific And Industrial Research Organisation Biocontrol agents for use in treatment of opportunistic infections
ES2243965T3 (es) * 1996-07-09 2005-12-01 Societe Des Produits Nestle S.A. Procedimiento de secado mediante pulverizacion.
JP4678896B2 (ja) * 1997-04-18 2011-04-27 ガネデン バイオテック，インコーポレイテッド 微生物感染を予防または制御するための共生Ｂａｃｉｌｌｕｓ芽胞の局所的使用
EP0904784A1 (en) * 1997-09-22 1999-03-31 N.V. Nutricia Probiotic nutritional preparation
WO1999017788A1 (en) * 1997-10-06 1999-04-15 Abbott Laboratories Composition of treatment of candidiasis
EP1364586A1 (en) * 2002-05-24 2003-11-26 Nestec S.A. Probiotics and oral tolerance

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422370A (en) * 1986-12-23 1995-06-06 Tristrata Inc Method of using 2-hydroxypropanoic acid (lactic acid) for the treatment of wrinkles
US5422370B1 (en) * 1986-12-23 1997-07-15 Tristrata Inc Method of using 2-hydroxypropanoic acid (lactic acid) for the treatment of wrinkles
US5324515A (en) * 1991-10-22 1994-06-28 Pacific Corporation Cosmetic material obtained from a lactic acid fermentation broth
US5578302A (en) * 1992-07-06 1996-11-26 Nestec S.A. Treatment of stomach ulcers
US5603930A (en) * 1992-07-06 1997-02-18 Nestec S.A. Lactobacillus johnsonii CNCM I-1225
US5635202A (en) * 1993-12-03 1997-06-03 Lafor Laboratories Limited Micro-encapsulated lactobacilli for medical applications
US5633012A (en) * 1993-12-03 1997-05-27 Lafor Laboratories Limited Micro-encapsulated lactobacilli for medical applications
US5614209A (en) * 1993-12-03 1997-03-25 Lafor Laboratories Limited Micro-encapsulated lactobacilli for medical applications
US5733568A (en) * 1993-12-03 1998-03-31 Lafor Laboratories Limited Micro-encapsulated lactobacilli for medical applications
US5614209B1 (en) * 1993-12-03 1998-08-04 Lafor Lab Ltd Micro-encapsulated lactobacilli for medical applications
US5981261A (en) * 1995-08-07 1999-11-09 Nestec, S.A. Micrococcus varians strains for producing bactericide compositions
US6270811B1 (en) * 1996-11-26 2001-08-07 1 Fois 1 Jour Concept Pharmaceutical, cosmetic composition with base of microbial culture mixed with an essential oil and an acid
US6645506B1 (en) * 1997-04-18 2003-11-11 Ganeden Biotech, Inc. Topical compositions containing extracellular products of Pseudomonas lindbergii and Emu oil

Cited By (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090098053A1 (en) * 2002-04-09 2009-04-16 Nestec S.A. La1 - the genome of a lactobacillus strain
US7875421B2 (en) * 2002-04-09 2011-01-25 Nestec S.A. La1—the genome of a lactobacillus strain
DE102004023612A1 (de) * 2004-05-10 2005-12-01 Helmut Meusel Verwendung von microbiellen Mischbiozönosen zur Behandlung von Entzündungen der Haut
WO2006013441A2 (en) * 2004-07-29 2006-02-09 Proge Farm S.R.L. Use of probiotic bacteria for the preparation of topical compositions for skin protection
WO2006013441A3 (en) * 2004-07-29 2006-04-06 Proge Farm Srl Use of probiotic bacteria for the preparation of topical compositions for skin protection
US20060171936A1 (en) * 2004-10-04 2006-08-03 L'oreal Cosmetic and/or dermatological composition for sensitive skin
FR2879452A1 (fr) * 2004-12-21 2006-06-23 Oreal Utilisation d'un extrait de bacterie filamenteuse non photosynthetique non fructifiante en tant qu'agent modulant l'adhesion de microorganismes cutanes
US20090232785A1 (en) * 2005-08-01 2009-09-17 L'oreal Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin
WO2007039086A1 (en) * 2005-09-19 2007-04-12 Basf Aktiengesellschaft Microorganisms inhibiting the formation of foot malodor
US20090130073A1 (en) * 2005-09-19 2009-05-21 Basf Se Microorganisms Inhibiting the Formation of Foot Malodor
US9314490B2 (en) 2006-11-06 2016-04-19 Nestec S.A. Biological effects of compositions of rosmarinic acid
US20100129324A1 (en) * 2006-11-06 2010-05-27 Vanessa Crespy biological effects of compositions comprising rosmarinic acid
US8932579B2 (en) * 2006-11-06 2015-01-13 Nestec S.A. Biological effects of compositions comprising rosmarinic acid
EP1987860A2 (fr) 2007-04-27 2008-11-05 L'oreal Film hydrosoluble cosmétique
US20090022700A1 (en) * 2007-04-27 2009-01-22 L'oreal Cosmetic water-soluble film
US20100305053A1 (en) * 2007-08-02 2010-12-02 L'oreal Use of hesperidin or of a derivative thereof for the prevention and/or treatment of slackened skin
US10238897B2 (en) * 2007-09-04 2019-03-26 L'oreal Use of a lysate of bifidobacterium species for treating sensitive skin
US20090068161A1 (en) * 2007-09-04 2009-03-12 L'oreal Use of a combination of hesperidin and of a microorganism for influencing the barrier function of the skin
US11154731B2 (en) 2007-09-04 2021-10-26 L'oreal Cosmetic use of Bifidobacterium species lysate for the treatment of dryness
US20090068160A1 (en) * 2007-09-04 2009-03-12 L'oreal Use of a lysate of bifidobacterium species for treating sensitive skin
US8951775B2 (en) 2008-07-29 2015-02-10 L'oreal Cosmetic use of microorganisms for the treatment of oily skin
US20110182861A1 (en) * 2008-07-29 2011-07-28 Isabelle Castiel Cosmetic use of microorganisms for the treatment of oily skin
EP2540818A1 (en) * 2008-09-04 2013-01-02 OM Pharma Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
EP2161329A1 (en) * 2008-09-04 2010-03-10 OM Pharma Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
US20100055082A1 (en) * 2008-09-04 2010-03-04 Jacques Alain Bauer Immunomodulatory extracts from lactobacillus bacteria and methods of manufacturing and use thereof
US20100278793A1 (en) * 2008-10-28 2010-11-04 L'oreal Treatment of greasy skin with a bacterial lystate
US9782611B2 (en) * 2008-10-28 2017-10-10 L'oreal Treatment of greasy skin with a bacterial lystate
US8481299B2 (en) 2009-03-04 2013-07-09 L'oreal Use of probiotic microorganisms to limit skin irritation
US20100226892A1 (en) * 2009-03-04 2010-09-09 L'oreal Use of probiotic microorganisms to limit skin irritation
AU2010247469B2 (en) * 2009-05-11 2014-12-18 Société des Produits Nestlé S.A. Lactobacillus johnsonii La1 NCC533 (CNCM I-1225) and immune disorders
US10576110B2 (en) 2009-05-11 2020-03-03 Societe Des Produits Nestle S.A. Lactobacillus johnsonii La1 NCC533 (CNCM I-1225) and immune disorders
TWI489988B (zh) * 2009-05-11 2015-07-01 耐斯泰克公司 約氏乳桿菌（Ｌａｃｔｏｂａｃｉｌｌｕｓ ｊｏｈｎｓｏｎｉｉ）Ｌａ１ ＮＣＣ ５３３（ＣＮＣＭ Ｉ－１２２５）及免疫疾病
US20140065209A1 (en) * 2011-05-03 2014-03-06 Dupont Nutrition Biosciences Aps Probiotic bacteria for the topical treatment of skin disorders
US20160008412A1 (en) * 2011-05-03 2016-01-14 Dupont Nutrition Biosciences Aps Probiotic bacteria for the topical treatment of skin disorders
US10555977B2 (en) 2011-05-03 2020-02-11 Dupont Nutrition Biosciences Aps Probiotic bacteria for the topical treatment of skin disorders
US20150093462A1 (en) * 2013-09-30 2015-04-02 Elc Management Llc Watery Lotion Skin Care Compositions And Methods
US9913800B2 (en) * 2015-04-28 2018-03-13 The Procter & Gamble Company Compositions and methods for improving skin health
US20160317432A1 (en) * 2015-04-28 2016-11-03 The Procter & Gamble Company Compositions And Methods For Improving Skin Health
WO2016176380A1 (en) * 2015-04-28 2016-11-03 The Procter & Gamble Company Compositions comprising whole, non-viable micrococcus for improving skin health
US20180140540A1 (en) * 2016-11-23 2018-05-24 Gojo Industries, Inc. Topical cleansing composition with prebiotic/probiotic additive
WO2019226002A1 (ko) * 2018-05-23 2019-11-28 주식회사 고바이오랩 락토바실러스 크리스파투스 kbl693 균주 및 그 용도
US11141444B2 (en) 2018-05-23 2021-10-12 Ko Biolabs, Inc. Lactobacillus crispatus KBL693 strain and use thereof
US11633439B2 (en) 2018-05-23 2023-04-25 Kobiolabs, Inc. Lactobacillus crispatus KBL693 strain and use thereof

Also Published As

Publication number Publication date
EP1110555A1 (fr) 2001-06-27
NO20022989D0 (no) 2002-06-20
DK1244463T3 (da) 2010-09-20
AU3011801A (en) 2001-07-03
JP2003518070A (ja) 2003-06-03
EP1244463A1 (fr) 2002-10-02
US8685389B2 (en) 2014-04-01
US9226943B2 (en) 2016-01-05
CN1434716A (zh) 2003-08-06
WO2001045721A1 (fr) 2001-06-28
RU2002119396A (ru) 2004-02-27
KR20020068382A (ko) 2002-08-27
MXPA02006222A (es) 2002-12-05
PL355411A1 (en) 2004-04-19
BR0016709A (pt) 2002-09-03
CN1434716B (zh) 2012-03-28
KR100797819B1 (ko) 2008-01-24
CA2395419C (en) 2010-07-06
CY1111029T1 (el) 2015-06-11
US20160082052A1 (en) 2016-03-24
US20060002910A1 (en) 2006-01-05
CN101385745A (zh) 2009-03-18
EP1244463B1 (fr) 2010-06-16
NO20022989L (no) 2002-08-16
PT1244463E (pt) 2010-09-07
ATE471152T1 (de) 2010-07-15
AR030925A1 (es) 2003-09-03
ES2345761T3 (es) 2010-10-01
CA2395419A1 (en) 2001-06-28
CN101385745B (zh) 2011-05-18
US20140079677A1 (en) 2014-03-20
JP4828759B2 (ja) 2011-11-30
DE60044566D1 (de) 2010-07-29

Similar Documents

Publication Publication Date Title
US9226943B2 (en) Lactobacillus johnsonii CNCM I-1225 for the anti-adhesion of skin pathogenic flora
AU2012203217B2 (en) Methods and means for protecting the skin against pathogenic microorganisms
US8481299B2 (en) Use of probiotic microorganisms to limit skin irritation
US8283136B2 (en) Skin treatment compositions
US20090035294A1 (en) Lipopolysaccharide fractions of vitreoscilla filiformis useful for stimulating the synthesis of anti-microbial peptides of the skin
AU2017202471B2 (en) Peptide for use in the treatment of skin conditions
KR102441226B1 (ko) 혼합 프로바이오틱스 발효물을 포함하는 피부 마이크로바이옴 개선용 조성물 및 이를 포함하는 화장료 조성물
CA2415918C (en) Agent for inhibiting adhesion of the pathogenic flora of the skin
US10953051B2 (en) Methods and means for protecting the skin against pathogenic microorganisms
KR20240008176A (ko) 신규한 락토바실러스 유산균 hk9 복합 발효물의 추출물을 포함하는 기능성 화장품 조성물

Legal Events

Date Code Title Description
AS Assignment

Owner name: NESTEC S.A., SWITZERLAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BAUR, MARKUS;ZINK, RALF;AUZANNEAU, ISABELLE;AND OTHERS;REEL/FRAME:013646/0178;SIGNING DATES FROM 20020702 TO 20021008

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION