UA76690C2 - Дріжджі, що зброджують ксилозу у етанол (варіанти), плазмідний вектор та спосіб зброджування ксилози у етанол - Google Patents
Дріжджі, що зброджують ксилозу у етанол (варіанти), плазмідний вектор та спосіб зброджування ксилози у етанол Download PDFInfo
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- UA76690C2 UA76690C2 UA98126403A UA98126403A UA76690C2 UA 76690 C2 UA76690 C2 UA 76690C2 UA 98126403 A UA98126403 A UA 98126403A UA 98126403 A UA98126403 A UA 98126403A UA 76690 C2 UA76690 C2 UA 76690C2
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- Prior art keywords
- yeast
- xylose
- ethanol
- genes
- dna
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1686596P | 1996-05-06 | 1996-05-06 | |
| PCT/US1997/007663 WO1997042307A1 (en) | 1996-05-06 | 1997-05-06 | Stable recombinant yeasts for fermenting xylose to ethanol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA76690C2 true UA76690C2 (uk) | 2006-09-15 |
Family
ID=21779420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UA98126403A UA76690C2 (uk) | 1996-05-06 | 1997-06-05 | Дріжджі, що зброджують ксилозу у етанол (варіанти), плазмідний вектор та спосіб зброджування ксилози у етанол |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US7527927B1 (forum.php) |
| EP (1) | EP0898616A4 (forum.php) |
| JP (2) | JP4321878B2 (forum.php) |
| CN (1) | CN1238490C (forum.php) |
| AU (1) | AU731102B2 (forum.php) |
| BR (1) | BR9710963A (forum.php) |
| CA (2) | CA2253581C (forum.php) |
| ID (1) | ID16873A (forum.php) |
| IN (1) | IN191596B (forum.php) |
| UA (1) | UA76690C2 (forum.php) |
| WO (1) | WO1997042307A1 (forum.php) |
Families Citing this family (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN191596B (forum.php) * | 1996-05-06 | 2003-12-06 | Purdue Research Foundation | |
| FI980551A7 (fi) | 1998-03-11 | 1999-09-12 | Valtion Teknillinen | Transformoidut mikro-organismit, joilla on parannettuja ominaisuuksia |
| WO1999054477A2 (en) | 1998-04-20 | 1999-10-28 | Forskarpatent I Syd Ab | Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates |
| US7537826B2 (en) | 1999-06-22 | 2009-05-26 | Xyleco, Inc. | Cellulosic and lignocellulosic materials and compositions and composites made therefrom |
| BR0110987B1 (pt) * | 2000-05-01 | 2014-07-15 | Alliance Sustainable Energy | Transposon para inserção estável de genes alheios dentro de um genoma bacteriano, vetor plasmídio, bactéria, e, mistura de reação compreendendo um meio fermentável |
| US7354755B2 (en) | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
| WO2001088094A1 (en) | 2000-05-15 | 2001-11-22 | Forskarpatent I Syd | A recombinant yeast for lignocellulose raw materials |
| ATE350492T1 (de) | 2001-02-28 | 2007-01-15 | Iogen Energy Corp | Methode zur behandlung von lignin- und zellulosehaltigen beschickungen zur erhöhten produktion von xylose und ethanol |
| ES2426966T3 (es) | 2003-05-02 | 2013-10-28 | Cargill, Incorporated | Especies de levadura genéticamente modificadas, y procesos de fermentación que emplean levaduras genéticamente modificadas |
| CN100339483C (zh) * | 2003-12-19 | 2007-09-26 | 首都师范大学 | 酵母菌利用木糖和葡萄糖生产酒精的方法 |
| EP1698702B1 (en) * | 2005-03-02 | 2012-02-22 | PharmedArtis GmbH | Recombinant protein expression system |
| US20150328347A1 (en) | 2005-03-24 | 2015-11-19 | Xyleco, Inc. | Fibrous materials and composites |
| CN1325633C (zh) * | 2005-08-08 | 2007-07-11 | 天津大学 | 甘油通道蛋白基因缺失降低甘油生成提高乙醇产量的酿酒酵母菌株及构建方法 |
| EP2029762A4 (en) | 2006-06-22 | 2010-04-28 | Iogen Energy Corp | ENZYME COMPOSITIONS FOR ENHANCED ENZYMATIC HYDROLYSIS OF CELLULOSE AND METHODS OF USE THEREOF |
| CN1966694B (zh) * | 2006-11-15 | 2010-05-12 | 山东大学 | 一种利用葡萄糖木糖共发酵生产酒精的方法 |
| US7897396B2 (en) * | 2007-10-30 | 2011-03-01 | E.I. Du Pont De Nemours And Company | Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate |
| CN101463328B (zh) * | 2007-12-19 | 2011-06-29 | 中国科学院微生物研究所 | 一株利用木糖发酵产乙醇的代谢工程酵母菌 |
| US9228178B2 (en) | 2008-05-30 | 2016-01-05 | Archer Daniels Midland Co. | Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha |
| CN101302535B (zh) * | 2008-06-30 | 2010-10-20 | 黑龙江大学 | 一种表达木酮糖激酶基因的质粒的构建方法 |
| WO2010151574A2 (en) | 2009-06-25 | 2010-12-29 | Shell Oil Company | Water injection systems and methods |
| CA2767361A1 (en) | 2009-07-09 | 2011-01-13 | Verdezyne, Inc. | Engineered microorganisms with enhanced fermentation activity |
| JP5649208B2 (ja) * | 2009-10-19 | 2015-01-07 | 国立大学法人 熊本大学 | キシロースからエタノールを発酵する酵母 |
| CA2719280A1 (en) | 2009-10-28 | 2011-04-28 | Erin Johnson | Novel ethanol-producing yeast |
| CN102947440B (zh) * | 2009-12-30 | 2015-04-08 | 埃欧金能源公司 | 呈现增强的木质纤维素水解产物发酵的改良酵母菌株 |
| US8759049B2 (en) | 2010-02-25 | 2014-06-24 | Iogen Energy Corporation | Method for the production of a fermentation product from a sugar hydrolysate |
| CN102220254B (zh) * | 2010-04-14 | 2013-04-17 | 新疆农业科学院生物质能源研究所 | 一种重组酿酒酵母工程菌株及其应用 |
| WO2011131674A1 (en) | 2010-04-21 | 2011-10-27 | Dsm Ip Assets B.V. | Process for the production of cells which are capable of converting arabinose |
| WO2012024698A1 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
| US8709770B2 (en) | 2010-08-31 | 2014-04-29 | Iogen Energy Corporation | Process for improving the hydrolysis of cellulose in high consistency systems using one or more unmixed and mixed hydrolysis reactors |
| US20130295631A1 (en) * | 2010-10-01 | 2013-11-07 | The Board Of Trustees Of The University Of Illinois | Combinatorial design of highly efficient heterologous pathways |
| WO2012047832A2 (en) | 2010-10-07 | 2012-04-12 | Shell Oil Company | Process for the production of alcohols from biomass |
| US9290821B2 (en) | 2010-11-05 | 2016-03-22 | Shell Oil Company | Treating biomass to produce materials useful for biofuels |
| WO2012067279A1 (ko) * | 2010-11-16 | 2012-05-24 | 서울대학교 산학협력단 | 토르(tor) 신호 전달 경로에 관여하는 유전자의 기능을 소실시킨 재조합 사카로마이세스 세레비지애를 이용하여 자일로오스로부터 에탄올을 생산하는 방법 |
| BR112013012130A2 (pt) | 2010-11-22 | 2017-10-31 | Cargill Inc | composições e métodos para titulação aumentada de etanol a partir de biomassa |
| JP5850418B2 (ja) * | 2010-12-03 | 2016-02-03 | 国立研究開発法人産業技術総合研究所 | キシロース代謝改変による効果的なエタノール生産法 |
| JP2012120491A (ja) * | 2010-12-09 | 2012-06-28 | Toyota Motor Corp | キシロースを含む培地における発酵培養方法 |
| US8609379B2 (en) | 2010-12-20 | 2013-12-17 | Shell Oil Company | Process for the production of alcohols from biomass |
| US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| TWI438274B (zh) * | 2011-06-03 | 2014-05-21 | Inst Nuclear Energy Res Atomic Energy Council | 一種木糖代謝菌之製備方法及該木糖代謝菌 |
| WO2013028701A1 (en) | 2011-08-22 | 2013-02-28 | Codexis, Inc. | Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity |
| EP2760997A4 (en) | 2011-09-30 | 2015-02-11 | Codexis Inc | MUSHROOM PROTEASE |
| PL2791344T3 (pl) | 2011-12-15 | 2016-08-31 | Shell Int Research | Sposób obróbki produktów ubocznych z wytwarzania etanolu |
| EP2847344B1 (en) | 2012-05-07 | 2019-07-24 | Shell International Research Maatschappij B.V. | Continuous or semi-continuous process for treating biomass |
| EP2850198B1 (en) | 2012-05-17 | 2020-06-17 | Shell International Research Maatschappij B.V. | Process for producing volatile organic compounds from biomass material |
| JP2015523061A (ja) | 2012-05-17 | 2015-08-13 | シエル・インターナシヨネイル・リサーチ・マーチヤツピイ・ベー・ウイＳｈｅｌｌ Ｉｎｔｅｒｎａｔｉｏｎａｌｅ Ｒｅｓｅａｒｃｈ Ｍａａｔｓｃｈａｐｐｉｊ Ｂｅｓｌｏｔｅｎｖｅｎｎｏｏｔｓｈａｐ | バイオマス材料を処理する方法およびシステム |
| SG195410A1 (en) * | 2012-05-25 | 2013-12-30 | Ngee Ann Polytechnic | Xylose fermenting yeast constructed using an modified genome shuffling method |
| CN103060217B (zh) * | 2012-11-29 | 2014-12-17 | 天津大学 | 一株高效代谢木糖的重组酵母菌株及用途 |
| CN103146741B (zh) * | 2013-02-01 | 2014-12-10 | 首都师范大学 | 三阶段基因转录调控提高纤维素乙醇产量的方法及基因工程菌株 |
| WO2014185635A1 (ko) * | 2013-05-16 | 2014-11-20 | 에스케이이노베이션 주식회사 | 자일로스로부터 에탄올을 생산할 수 있는 재조합 효모 및 이를 이용한 에탄올 생산방법 |
| BR112016023823A2 (pt) | 2014-04-17 | 2017-08-15 | Shell Int Research | método para produção de um produto de fermentação |
| US10619173B2 (en) | 2014-07-22 | 2020-04-14 | Iogen Corporation | Process for using biogenic carbon dioxide derived from non-fossil organic material |
| US10640793B2 (en) | 2014-07-22 | 2020-05-05 | Iogen Corporation | Process for using biogenic carbon dioxide derived from non-fossil organic material |
| EP3180451A1 (en) | 2014-08-14 | 2017-06-21 | Shell Internationale Research Maatschappij B.V. | Improved process for treating biomass to produce materials useful for biofuels |
| WO2016025678A1 (en) | 2014-08-14 | 2016-02-18 | Shell Oil Company | Process for preparing furfural from biomass |
| US9546386B2 (en) * | 2014-09-03 | 2017-01-17 | Tekkware, Inc. | Glucose and xylose co-fermenting microorganism that expresses active glucoamylase |
| US11434509B2 (en) | 2014-12-08 | 2022-09-06 | Iogen Corporation | Process for using biogenic carbon dioxide derived from non-fossil organic material |
| CN104561082B (zh) * | 2015-01-06 | 2018-03-20 | 江南大学 | 一株能够利用木糖的酵母Candida jeffriesii的表达系统 |
| CN104561081B (zh) * | 2015-01-06 | 2019-03-15 | 江南大学 | 一株能够利用木糖的酵母Candida amazonensis的表达系统 |
| CN104630258B (zh) * | 2015-01-06 | 2018-08-14 | 江南大学 | 一种酿酒酵母基因表达系统及其构建与应用 |
| WO2016145528A1 (en) | 2015-03-16 | 2016-09-22 | Iogen Corporation | Lignocellulosic conversion process comprising sulfur dioxide and/or sulfurous acid pretreatment |
| WO2016145531A1 (en) | 2015-03-16 | 2016-09-22 | Iogen Corporation | Sulfur dioxide and/or sulfurous acid pretreatment with sulfur dioxide recovery |
| WO2016145527A1 (en) | 2015-03-16 | 2016-09-22 | Iogen Corporation | Process comprising acid pretreatment and enzymatic hydrolysis |
| CA2998414C (en) | 2015-09-24 | 2022-09-20 | Iogen Corporation | Wet oxidation of biomass |
| US10889795B2 (en) | 2015-11-25 | 2021-01-12 | Iogen Energy Corporation | System and method for cooling pretreated biomass |
| CA3006672A1 (en) | 2015-12-18 | 2017-06-22 | Iogen Corporation | Sulfur dioxide and/or sulfurous acid pretreatment |
| BR112018016366A2 (pt) | 2016-02-10 | 2018-12-18 | Iogen Corp | processos para hidrolisar biomassa lignocelulósica e para pré-tratamento de biomassa lignocelulósica. |
| EP3452458A1 (en) | 2016-05-03 | 2019-03-13 | Shell Internationale Research Maatschappij B.V. | Lignin-based solvents and methods for their preparation |
| KR102062144B1 (ko) * | 2016-06-23 | 2020-01-03 | 안동대학교 산학협력단 | 마를 포함하는 알코올 발효 촉진용 배지 조성물 및 이를 이용한 마 발효주 |
| WO2018018111A1 (pt) * | 2016-07-28 | 2018-02-01 | Universidade Estadual De Campinas - Unicamp | Levedura industrial geneticamente modificada lvy127 com a via oxi-redutiva de conversão de xilose, cassetes de expressão gênica, processo de obtenção de etanol 2g e uso da levedura lvy127 |
| BR102016025442A2 (pt) * | 2016-10-31 | 2018-10-02 | Universidade Estadual De Campinas - Unicamp | levedura industrial geneticamente modificada lvy142 com a via oxi-redutiva de conversão de xilose, cassete de expressão gênica, processo de obtenção de etanol 2g e uso da levedura lvy142 |
| WO2018085174A1 (en) | 2016-11-01 | 2018-05-11 | Shell Oil Company | Process for the recovery of furfural |
| US10501430B2 (en) | 2016-11-01 | 2019-12-10 | Shell Oil Company | Process for the recovery of furfural |
| WO2018085176A1 (en) | 2016-11-01 | 2018-05-11 | Shell Oil Company | Process for the recovery of furfural |
| WO2018085179A1 (en) | 2016-11-01 | 2018-05-11 | Shell Oil Company | Process for the recovery of furfural |
| ES2904836T3 (es) | 2016-12-20 | 2022-04-06 | Novozymes As | Cepas de levaduras recombinantes para la fermentación de pentosa |
| WO2019006312A1 (en) | 2017-06-30 | 2019-01-03 | Myriant Corporation | MICROORGANISM HAVING A NUMBER OF STABILIZED COPIES OF A FUNCTIONAL DNA SEQUENCE AND ASSOCIATED METHODS |
| CA3078822A1 (en) | 2017-11-09 | 2019-05-16 | Iogen Corporation | Low temperature sulfur dioxide pretreatment |
| US11345935B2 (en) | 2017-11-09 | 2022-05-31 | Iogen Corporation | Low temperature pretreatment with sulfur dioxide |
| US11312977B2 (en) | 2018-04-06 | 2022-04-26 | Iogen Corporation | Pretreatment with lignosulfonic acid |
| WO2020171831A1 (en) | 2019-02-22 | 2020-08-27 | Tekkware, Inc. | Biomass with bioengineered yeast, associated organic compounds, and related methods |
| US11787773B2 (en) | 2019-05-22 | 2023-10-17 | Shell Usa, Inc. | Process for the production of furfural |
| AU2024212235A1 (en) | 2023-01-23 | 2025-07-03 | Shell Internationale Research Maatschappij B.V. | Method for treating grains to produce material useful for chemicals and biofuels |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4009676A1 (de) | 1990-03-26 | 1991-10-02 | Rhein Biotech Proz & Prod Gmbh | Dna-sequenz, umfassend ein fuer xylosereduktase und/oder xylitoldehydrogenase codierendes strukturgen |
| US5866382A (en) * | 1990-04-06 | 1999-02-02 | Xyrofin Oy | Xylose utilization by recombinant yeasts |
| FI901771A7 (fi) | 1990-04-06 | 1991-10-07 | Valtion Teknillinen | Anvaendning av xylos hos hybridjaest. |
| US5789210A (en) * | 1993-11-08 | 1998-08-04 | Purdue Research Foundation | Recombinant yeasts for effective fermentation of glucose and xylose |
| IN191596B (forum.php) * | 1996-05-06 | 2003-12-06 | Purdue Research Foundation |
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- 1997-05-06 CA CA2253581A patent/CA2253581C/en not_active Expired - Fee Related
- 1997-05-06 BR BR9710963-0A patent/BR9710963A/pt not_active Application Discontinuation
- 1997-05-06 JP JP54015397A patent/JP4321878B2/ja not_active Expired - Fee Related
- 1997-05-06 US US09/180,340 patent/US7527927B1/en not_active Expired - Fee Related
- 1997-05-06 AU AU28301/97A patent/AU731102B2/en not_active Ceased
- 1997-05-06 CA CA2661090A patent/CA2661090C/en not_active Expired - Fee Related
- 1997-05-06 ID IDP971509A patent/ID16873A/id unknown
- 1997-05-06 EP EP97922698A patent/EP0898616A4/en not_active Withdrawn
- 1997-05-06 WO PCT/US1997/007663 patent/WO1997042307A1/en not_active Application Discontinuation
- 1997-05-06 CN CNB971961956A patent/CN1238490C/zh not_active Expired - Lifetime
- 1997-06-05 UA UA98126403A patent/UA76690C2/uk unknown
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2009
- 2009-02-12 JP JP2009029455A patent/JP2009148277A/ja active Pending
- 2009-03-27 US US12/412,524 patent/US8652772B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20090246857A1 (en) | 2009-10-01 |
| JP2009148277A (ja) | 2009-07-09 |
| BR9710963A (pt) | 2001-07-31 |
| US7527927B1 (en) | 2009-05-05 |
| AU2830197A (en) | 1997-11-26 |
| ID16873A (id) | 1997-11-20 |
| CA2661090A1 (en) | 1997-11-13 |
| CA2253581A1 (en) | 1997-11-13 |
| WO1997042307A1 (en) | 1997-11-13 |
| EP0898616A1 (en) | 1999-03-03 |
| JP4321878B2 (ja) | 2009-08-26 |
| CA2661090C (en) | 2013-02-05 |
| CN1225125A (zh) | 1999-08-04 |
| US8652772B2 (en) | 2014-02-18 |
| AU731102B2 (en) | 2001-03-22 |
| CN1238490C (zh) | 2006-01-25 |
| EP0898616A4 (en) | 2000-12-06 |
| JP2000509988A (ja) | 2000-08-08 |
| IN191596B (forum.php) | 2003-12-06 |
| CA2253581C (en) | 2012-04-17 |
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