TWI456054B - 細胞培養容器之製法 - Google Patents

細胞培養容器之製法 Download PDF

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Publication number
TWI456054B
TWI456054B TW096114771A TW96114771A TWI456054B TW I456054 B TWI456054 B TW I456054B TW 096114771 A TW096114771 A TW 096114771A TW 96114771 A TW96114771 A TW 96114771A TW I456054 B TWI456054 B TW I456054B
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TW
Taiwan
Prior art keywords
cell
cell culture
concave portion
concave
culture vessel
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TW096114771A
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English (en)
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TW200808958A (en
Inventor
Yuji Morimoto
Taiji Nishi
Taisuke Kamada
Go Tazaki
Motohiro Fukuda
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Kuraray Co
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Publication of TW200808958A publication Critical patent/TW200808958A/zh
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Publication of TWI456054B publication Critical patent/TWI456054B/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (4)

  1. 一種細胞培養容器之製法，其係於用以培養細胞之表面上具有凹凸圖案的細胞培養容器之製法，具備下列步驟：(1)形成該凹凸圖案之凹部的步驟，該凹凸圖案之凹部包含：細胞培養部、連通於該細胞培養部之微通路、及經由該微通路連通於該細胞培養部之導入領域；(2)將含有細胞接著誘導物質之液體滴入該導入領域，利用毛細現象而將含有細胞接著誘導物質之液體僅導入於該凹部的步驟；及(3)使導入於該凹部之該含有細胞接著誘導物質之液體乾燥，而將細胞接著誘導物質僅被覆於該凹部之底部及側壁之步驟；其於形成該微通路之際，該細胞培養部之底面寬度為相當於培養細胞直徑的1.0倍~20倍，該側壁之高度為相當於培養細胞直徑的0.5倍~10倍，該微通路之底面寬度為相當於培養細胞直徑的0.1倍~10倍。
  2. 如申請專利範圍第1項之細胞培養容器之製法，其中進行該凹部之表面處理，於其上導入細胞接著誘導物質。
  3. 如申請專利範圍第2項之細胞培養容器之製法，其中該表面處理為親水化處理。
  4. 如申請專利範圍第1或2項之細胞培養容器之製法，其中至少用以配置該細胞的凹凸圖案領域為透明的。
TW096114771A 2006-04-28 2007-04-26 細胞培養容器之製法 TWI456054B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2006124657 2006-04-28

Publications (2)

Publication Number Publication Date
TW200808958A TW200808958A (en) 2008-02-16
TWI456054B true TWI456054B (zh) 2014-10-11

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TW096114771A TWI456054B (zh) 2006-04-28 2007-04-26 細胞培養容器之製法

Country Status (5)

Country Link
US (1) US8652833B2 (zh)
EP (1) EP2014763B1 (zh)
JP (1) JP5281886B2 (zh)
TW (1) TWI456054B (zh)
WO (1) WO2007126127A1 (zh)

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WO2008156041A1 (ja) 2007-06-18 2008-12-24 Kuraray Co., Ltd. 細胞培養容器及び細胞培養方法
DE102008018170B4 (de) 2008-04-03 2010-05-12 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Mikrofluidisches System und Verfahren zum Aufbau und zur anschließenden Kultivierung sowie nachfolgender Untersuchung von komplexen Zellanordnungen
KR101746864B1 (ko) * 2008-10-24 2017-06-14 주식회사 쿠라레 세포 보존 방법 및 세포 수송 방법
KR20110074773A (ko) * 2008-10-24 2011-07-01 가부시키가이샤 구라레 세포 배양 키트, 스크리닝 방법 및 세포 배양 키트의 제조 방법
JP2010136706A (ja) * 2008-12-15 2010-06-24 Covalent Materials Corp 細胞培養担体
JP5407345B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
JP5407343B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
JP5407344B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
EP2219027A1 (en) * 2009-02-12 2010-08-18 Centre National de la Recherche Scientifique Devices and methods for observing the cell division
DE102009039956A1 (de) 2009-08-27 2011-03-10 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Mikrofluidisches System und Verfahren zu dessen Herstellung
WO2012032646A1 (ja) * 2010-09-10 2012-03-15 株式会社島津製作所 細胞培養デバイス及び細胞培養方法
EP2825626B1 (en) * 2012-03-14 2018-04-11 Centre National De La Recherche Scientifique Devices and methods for observing eukaryotic cells without cell wall
CA2867636A1 (en) * 2012-03-29 2013-10-03 Centre National De La Recherche Scientifique Immobilizing a plurality of oblong shaped cells or invertebrate embryos on a device
JP5713086B2 (ja) * 2013-11-06 2015-05-07 大日本印刷株式会社 生体組織の作製方法
CN108558408B (zh) * 2018-06-05 2020-12-01 李钰龙 一种一体化微通道板的制备方法

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US20050279730A1 (en) * 2004-02-19 2005-12-22 Hideyuki Miyake Method for manufacturing cell culture substrate

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US20050279730A1 (en) * 2004-02-19 2005-12-22 Hideyuki Miyake Method for manufacturing cell culture substrate

Also Published As

Publication number Publication date
EP2014763A4 (en) 2012-05-30
EP2014763B1 (en) 2015-11-18
JP5281886B2 (ja) 2013-09-04
WO2007126127A1 (ja) 2007-11-08
US8652833B2 (en) 2014-02-18
US20090075363A1 (en) 2009-03-19
TW200808958A (en) 2008-02-16
EP2014763A1 (en) 2009-01-14
JPWO2007126127A1 (ja) 2009-09-17

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