TWI275796B - Method for measuring physiologically active sample substance by the use of porous filter - Google Patents
Method for measuring physiologically active sample substance by the use of porous filter Download PDFInfo
- Publication number
- TWI275796B TWI275796B TW090118884A TW90118884A TWI275796B TW I275796 B TWI275796 B TW I275796B TW 090118884 A TW090118884 A TW 090118884A TW 90118884 A TW90118884 A TW 90118884A TW I275796 B TWI275796 B TW I275796B
- Authority
- TW
- Taiwan
- Prior art keywords
- solution
- ligand
- physiologically active
- substance
- sample
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 63
- 239000000126 substance Substances 0.000 title claims abstract description 49
- 239000003446 ligand Substances 0.000 claims abstract description 89
- 239000013543 active substance Substances 0.000 claims abstract description 45
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 230000000694 effects Effects 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 108
- 239000000523 sample Substances 0.000 claims description 63
- 239000013566 allergen Substances 0.000 claims description 48
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 26
- 238000005259 measurement Methods 0.000 claims description 26
- 230000001745 anti-biotin effect Effects 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 238000012360 testing method Methods 0.000 claims description 16
- 238000009739 binding Methods 0.000 claims description 14
- 230000027455 binding Effects 0.000 claims description 13
- 229960002685 biotin Drugs 0.000 claims description 13
- 235000020958 biotin Nutrition 0.000 claims description 13
- 239000011616 biotin Substances 0.000 claims description 13
- 239000012488 sample solution Substances 0.000 claims description 8
- 230000009870 specific binding Effects 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 239000012085 test solution Substances 0.000 claims description 2
- 108700028369 Alleles Proteins 0.000 claims 1
- 230000035479 physiological effects, processes and functions Effects 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 28
- 239000000203 mixture Substances 0.000 abstract description 10
- 230000037074 physically active Effects 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 description 75
- 239000002504 physiological saline solution Substances 0.000 description 18
- 239000000427 antigen Substances 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 17
- 102000036639 antigens Human genes 0.000 description 16
- 108091007433 antigens Proteins 0.000 description 16
- 108010090804 Streptavidin Proteins 0.000 description 13
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- 239000000872 buffer Substances 0.000 description 11
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- 239000008280 blood Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 description 9
- 108090001008 Avidin Proteins 0.000 description 7
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- 244000195452 Wasabia japonica Species 0.000 description 6
- 235000000760 Wasabia japonica Nutrition 0.000 description 6
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- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
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- 238000002372 labelling Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000013076 target substance Substances 0.000 description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
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- 239000012086 standard solution Substances 0.000 description 4
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
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- 239000003085 diluting agent Substances 0.000 description 3
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- 230000001900 immune effect Effects 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 244000004281 Eucalyptus maculata Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000006096 absorbing agent Substances 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
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- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
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- 235000010469 Glycine max Nutrition 0.000 description 1
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- 235000007164 Oryza sativa Nutrition 0.000 description 1
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- 241000224526 Trichomonas Species 0.000 description 1
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- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
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- 238000001179 sorption measurement Methods 0.000 description 1
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- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
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- 230000009385 viral infection Effects 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
- VWQVUPCCIRVNHF-UHFFFAOYSA-N yttrium atom Chemical compound [Y] VWQVUPCCIRVNHF-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000045380A JP4545869B2 (ja) | 2000-02-23 | 2000-02-23 | 多孔性フィルタを用いる生理活性試料物質の測定方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TWI275796B true TWI275796B (en) | 2007-03-11 |
Family
ID=18567975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW090118884A TWI275796B (en) | 2000-02-23 | 2001-08-02 | Method for measuring physiologically active sample substance by the use of porous filter |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP4545869B2 (https=) |
| KR (1) | KR20020086558A (https=) |
| AU (1) | AU2001232350A1 (https=) |
| TW (1) | TWI275796B (https=) |
| WO (1) | WO2001063283A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4739755B2 (ja) * | 2002-10-11 | 2011-08-03 | ゼットビーエックス コーポレーション | 診断装置 |
| JP4771941B2 (ja) | 2004-04-21 | 2011-09-14 | 株式会社エイアンドティー | 免疫学的定量法と試薬 |
| WO2006094665A1 (en) | 2005-03-08 | 2006-09-14 | Kenton Srl | Chimeric recombinant antigens of toxoplasma gondii |
| JP2007121095A (ja) * | 2005-10-27 | 2007-05-17 | Toyobo Co Ltd | 非特異反応が抑制されるように改良された測定容器 |
| JP4904182B2 (ja) * | 2007-03-19 | 2012-03-28 | 財団法人電力中央研究所 | カドミウム、鉛、又は３価クロムの免疫学的定量方法及びそれらの免疫学的定量装置、並びに、それらに用いるカドミウム、鉛、又は３価クロム錯体固定化膜 |
| JP5118479B2 (ja) * | 2007-12-28 | 2013-01-16 | 田中貴金属工業株式会社 | イムノクロマト分析法及びイムノクロマト分析キット |
| EP2108656A1 (en) | 2008-03-19 | 2009-10-14 | Beninati, Concetta | Antigenic protein fragments of streptococcus pneumoniae |
| WO2010041595A1 (ja) * | 2008-10-08 | 2010-04-15 | 東洋紡績株式会社 | 生理活性物質測定システム |
| JP5716501B2 (ja) * | 2011-04-04 | 2015-05-13 | 東洋紡株式会社 | 検体の前処理方法 |
| JP6531307B2 (ja) * | 2015-04-08 | 2019-06-19 | 株式会社パートナーファーム | 固相反応容器及びこれを用いた測定方法 |
| JP6943371B2 (ja) * | 2017-04-19 | 2021-09-29 | 国立大学法人福井大学 | 尿中の急性腎障害マーカーの測定方法 |
| ES3035098T3 (en) | 2018-02-02 | 2025-08-28 | Nippon Chemiphar Co | Biochemical reaction substrate and analysis device |
| KR20220041854A (ko) | 2019-08-07 | 2022-04-01 | 가부시키가이샤 파트너 펌 | 고상 반응 용기 및 이것을 사용한 측정 방법 |
| WO2021095858A1 (ja) * | 2019-11-14 | 2021-05-20 | インターメディック株式会社 | 生理活性物質の測定方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4496654A (en) * | 1983-04-08 | 1985-01-29 | Quidel | Detection of HCG with solid phase support having avidin coating |
| US4632901A (en) * | 1984-05-11 | 1986-12-30 | Hybritech Incorporated | Method and apparatus for immunoassays |
| DE3620653A1 (de) * | 1986-06-20 | 1987-12-23 | Boehringer Mannheim Gmbh | Verfahren zur herstellung eines immunreaktiven traegermaterials fuer die heterogene immunologische analyse |
| CA1333883C (en) * | 1988-04-07 | 1995-01-10 | Shashidhara H. M. Murthy | Immunoassay utilizing biotin bridge with universal solid phase |
| JPH01312463A (ja) * | 1988-06-10 | 1989-12-18 | Dai Ichi Pure Chem Co Ltd | 免疫学的測定法 |
| JP3134231B2 (ja) * | 1991-04-16 | 2001-02-13 | 東洋紡績株式会社 | 固相生物学的特異反応測定法およびそのための器具 |
| JPH10253632A (ja) * | 1997-03-10 | 1998-09-25 | Nissui Pharm Co Ltd | 分析方法、キット及び装置 |
| JPH11201969A (ja) * | 1998-01-14 | 1999-07-30 | Wakamoto Pharmaceut Co Ltd | 簡易遊離ヘモグロビン測定方法および測定用キット |
| JPH11326326A (ja) * | 1998-05-13 | 1999-11-26 | Dainippon Printing Co Ltd | 免疫検査体 |
| JP2000088851A (ja) * | 1998-09-10 | 2000-03-31 | Internatl Reagents Corp | 生物学的特異反応測定法及び装置 |
-
2000
- 2000-02-23 JP JP2000045380A patent/JP4545869B2/ja not_active Expired - Lifetime
-
2001
- 2001-02-20 KR KR1020027010881A patent/KR20020086558A/ko not_active Ceased
- 2001-02-20 WO PCT/JP2001/001196 patent/WO2001063283A1/ja not_active Ceased
- 2001-02-20 AU AU2001232350A patent/AU2001232350A1/en not_active Abandoned
- 2001-08-02 TW TW090118884A patent/TWI275796B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001063283A1 (en) | 2001-08-30 |
| JP2001235471A (ja) | 2001-08-31 |
| AU2001232350A1 (en) | 2001-09-03 |
| JP4545869B2 (ja) | 2010-09-15 |
| KR20020086558A (ko) | 2002-11-18 |
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