SG186876A1 - New pcr sequencing method and use thereof in hla genotyping - Google Patents

New pcr sequencing method and use thereof in hla genotyping Download PDF

Info

Publication number
SG186876A1
SG186876A1 SG2012096616A SG2012096616A SG186876A1 SG 186876 A1 SG186876 A1 SG 186876A1 SG 2012096616 A SG2012096616 A SG 2012096616A SG 2012096616 A SG2012096616 A SG 2012096616A SG 186876 A1 SG186876 A1 SG 186876A1
Authority
SG
Singapore
Prior art keywords
hla
pcr
seq
sequencing
pcr primers
Prior art date
Application number
SG2012096616A
Other languages
English (en)
Inventor
Jian Li
Shiping Chen
Xiandong Zhang
Ying Liu
Caifen Zhang
Tao Liu
Meiru Zhao
Original Assignee
Bgi Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201010213717.6A external-priority patent/CN101921840B/zh
Priority claimed from CN 201010213721 external-priority patent/CN101921842B/zh
Priority claimed from CN201010213719.5A external-priority patent/CN101921841B/zh
Priority claimed from PCT/CN2010/002149 external-priority patent/WO2012083505A1/zh
Priority claimed from PCT/CN2010/002150 external-priority patent/WO2012083506A1/zh
Application filed by Bgi Shenzhen Co Ltd filed Critical Bgi Shenzhen Co Ltd
Publication of SG186876A1 publication Critical patent/SG186876A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
SG2012096616A 2010-06-30 2011-06-30 New pcr sequencing method and use thereof in hla genotyping SG186876A1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN201010213717.6A CN101921840B (zh) 2010-06-30 2010-06-30 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
CN 201010213721 CN101921842B (zh) 2010-06-30 2010-06-30 Hla-a,b基因分型用pcr引物及其使用方法
CN201010213719.5A CN101921841B (zh) 2010-06-30 2010-06-30 基于Illumina GA测序技术的HLA基因高分辨率分型方法
PCT/CN2010/002149 WO2012083505A1 (zh) 2010-12-24 2010-12-24 Hla-c基因分型的方法及其相关引物
PCT/CN2010/002150 WO2012083506A1 (zh) 2010-12-24 2010-12-24 Hla-dqb1基因分型的方法及其相关引物
PCT/CN2011/076688 WO2012000445A1 (zh) 2010-06-30 2011-06-30 新的的pcr测序方法及其在hla基因分型中的应用

Publications (1)

Publication Number Publication Date
SG186876A1 true SG186876A1 (en) 2013-02-28

Family

ID=45401405

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2012096616A SG186876A1 (en) 2010-06-30 2011-06-30 New pcr sequencing method and use thereof in hla genotyping

Country Status (12)

Country Link
US (1) US9957564B2 (https=)
EP (1) EP2599877B1 (https=)
JP (1) JP5968879B2 (https=)
KR (1) KR101709826B1 (https=)
AU (1) AU2011274090B2 (https=)
BR (1) BR112012032586B1 (https=)
CA (1) CA2803940C (https=)
DK (1) DK2599877T3 (https=)
MY (1) MY173793A (https=)
RU (1) RU2587606C2 (https=)
SG (1) SG186876A1 (https=)
WO (1) WO2012000445A1 (https=)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3597772A1 (en) * 2013-04-17 2020-01-22 Agency For Science, Technology And Research Method for generating extended sequence reads
JP6308724B2 (ja) * 2013-05-09 2018-04-11 ジェノダイブファーマ株式会社 Hla遺伝子のマルチプレックスdnaタイピング方法及びキット
US10508311B2 (en) * 2013-08-26 2019-12-17 The Translational Genomics Research Institute Single molecule-overlapping read analysis for minor variant mutation detection in pathogen samples
GB201409282D0 (en) 2014-05-23 2014-07-09 Univ Sydney Tech Sequencing process
EP3842544A1 (en) * 2014-09-05 2021-06-30 QIAGEN GmbH Preparation of adapter-ligated amplicons
DK3192869T3 (da) * 2014-09-12 2019-05-20 Mgi Tech Co Ltd Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
CN105400864B (zh) * 2014-09-12 2020-04-14 深圳华大基因股份有限公司 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
CA2971589C (en) 2014-12-18 2021-09-28 Edico Genome Corporation Chemically-sensitive field effect transistor
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
WO2016124069A1 (zh) * 2015-02-04 2016-08-11 深圳华大基因研究院 一种构建长片段测序文库的方法
KR101651817B1 (ko) * 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
US20190048413A1 (en) * 2016-02-23 2019-02-14 Novozymes A/S Improved next-generation sequencing
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
US11441188B2 (en) 2016-11-15 2022-09-13 Quest Diagnostics Investments Llc Methods for detecting DNA mutations using Mitra tip extraction
WO2018093723A1 (en) * 2016-11-15 2018-05-24 Quest Diagnostics Investments Llc Methods for detecting cystic fibrosis mutations using mitra tip extraction
EP3626835A1 (en) 2018-09-18 2020-03-25 Sistemas Genómicos, S.L. Method for genotypically identifying both alleles of at least one locus of a subject's hla gene
RU2703394C1 (ru) * 2018-10-24 2019-10-16 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Способ выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней
RU2703401C1 (ru) * 2018-10-24 2019-10-16 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Тест-система для выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней
FR3100820B1 (fr) 2019-09-12 2024-06-28 Bionobis Procédé de génotypage adapté au traitement d’un grand nombre d’échantillons, notamment en cas de polymorphisme élevé
FR3106596B1 (fr) 2020-01-27 2022-12-02 Bionobis Procede de genotypage hla simple et rapide
CN111235266B (zh) * 2020-03-10 2023-12-01 广州医科大学附属第二医院 Hla亚型检测试剂盒及其应用
FR3110178B1 (fr) * 2020-05-18 2022-05-27 Bionobis Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients
CN112553313A (zh) * 2020-12-30 2021-03-26 武汉康圣达医学检验所有限公司 用于Sanger法的测序反应产物的变性方法
KR102507110B1 (ko) * 2022-02-15 2023-03-07 주식회사 네오젠티씨 주조직 적합성 복합체의 타입들을 분석하기 위한 방법 및 장치

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07500734A (ja) 1991-10-31 1995-01-26 ユニヴァーシティ オブ ピッツバーグ オリゴヌクレオチドプローブのポリマーを用いる核酸配列の検出方法
US5580730A (en) * 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
US6287764B1 (en) * 1997-02-11 2001-09-11 William H. Hildebrand Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing
EP0953650A1 (en) * 1998-04-20 1999-11-03 Innogenetics N.V. Method for typing of HLA alleles
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
US7300755B1 (en) * 2003-05-12 2007-11-27 Fred Hutchinson Cancer Research Center Methods for haplotyping genomic DNA
EP1711621A4 (en) * 2003-10-28 2008-06-18 Dynal Biotech Llc PRIMERS, METHODS AND KITS FOR THE AMPLIFICATION OR DETECTION OF HUMAN LEUKOCYTE ANTIGEN ALLELES
WO2006122215A2 (en) * 2005-05-10 2006-11-16 State Of Oregon Acting By & Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods of mapping polymorphisms and polymorphism microarrays
WO2007044091A2 (en) * 2005-06-02 2007-04-19 Fluidigm Corporation Analysis using microfluidic partitioning devices
WO2007037678A2 (en) * 2005-09-29 2007-04-05 Keygene N.V. High throughput screening of mutagenized populations
JP5166276B2 (ja) 2005-11-14 2013-03-21 ケイヘーネ・エヌ・ブイ トランスポゾンタギング集団のハイスループットスクリーニングおよび挿入部位の大規模並行配列特定のための方法
CN103937899B (zh) * 2005-12-22 2017-09-08 凯津公司 用于基于aflp的高通量多态性检测的方法
WO2007140540A2 (en) * 2006-06-09 2007-12-13 Conexio 4 Pty Ltd Identification of a nucleic acid molecule
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
CN102124125A (zh) 2007-10-16 2011-07-13 霍夫曼-拉罗奇有限公司 通过克隆性测序的高分辨率、高通量hla基因分型
RU2393231C1 (ru) * 2008-12-29 2010-06-27 Федеральное государственное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб") Способ определения генетического родства штаммов холерных вибрионов методом секвенирования генов, фланкирующих кластер генов биосинтеза о-антигена
CN101921840B (zh) * 2010-06-30 2014-06-25 深圳华大基因科技有限公司 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
CN101921841B (zh) 2010-06-30 2014-03-12 深圳华大基因科技有限公司 基于Illumina GA测序技术的HLA基因高分辨率分型方法
CN101921842B (zh) * 2010-06-30 2013-08-07 深圳华大基因科技有限公司 Hla-a,b基因分型用pcr引物及其使用方法

Also Published As

Publication number Publication date
DK2599877T3 (da) 2017-11-20
CA2803940A1 (en) 2012-01-05
CA2803940C (en) 2019-07-02
AU2011274090A1 (en) 2013-02-07
RU2013103795A (ru) 2014-08-20
MY173793A (en) 2020-02-24
EP2599877A4 (en) 2014-01-01
AU2011274090B2 (en) 2015-04-09
KR20130038353A (ko) 2013-04-17
KR101709826B1 (ko) 2017-02-24
US20130237432A1 (en) 2013-09-12
BR112012032586B1 (pt) 2021-08-17
BR112012032586A2 (pt) 2019-07-30
US9957564B2 (en) 2018-05-01
JP5968879B2 (ja) 2016-08-10
WO2012000445A1 (zh) 2012-01-05
JP2013529472A (ja) 2013-07-22
EP2599877A1 (en) 2013-06-05
EP2599877B1 (en) 2017-09-27
RU2587606C2 (ru) 2016-06-20

Similar Documents

Publication Publication Date Title
AU2011274090B2 (en) New PCR sequencing method and use thereof in HLA genotyping
AU2007313472B2 (en) Population scale HLA-typing and uses thereof
CN108460246A (zh) 一种基于三代测序平台的hla基因分型方法
WO2012000150A1 (zh) Hla-a,b基因分型用pcr引物及其使用方法
CN116179671A (zh) 一种用于hla基因分型的扩增引物组、试剂盒及方法
WO2011030159A1 (en) Method using hla epitope determination
Dunn Novel approaches and technologies in molecular HLA typing
CN103270170B (zh) Hla-dqb1基因分型的方法及其相关引物
TWI542696B (zh) HLA - C genotyping and its related primers
JP2009261358A (ja) Hla−drb1遺伝子を含むヒト遺伝子のアレル多型のタイピング方法及びこれに用いるキット
WO2017135396A1 (ja) Pcrを用いないキャプチャー法によるhla遺伝子タイピング用プローブセット及びそれを用いたタイピング方法
WO2007056904A1 (en) Microarrays for genotyping and methods of use
CN108660252A (zh) 一种基于焦磷酸测序的人类免疫缺陷病毒耐药性分析方法
Baek et al. The HLA‐B* 07: 457 allele identified in a volunteer donor for hematopoietic stem cell transplant.
RU2837865C1 (ru) Способ секвенирования экзонов гена HLA-DPA1 и набор синтетических олигонуклеотидов для его реализации
Tambe et al. Characterization of the novel HLA‐B* 58: 01: 01: 19 allele by next‐generation sequencing.
De Santis et al. KIR Genotyping
JP2010162020A (ja) 新規hla−drb1遺伝子およびその用途
AU2013273684A1 (en) Population scale hla-typing and uses thereof
HK1185115B (en) Method for hla-dqb1 genotyping and related primers thereof
HK1185114B (en) Method for hla-c genotyping and related primers thereof
JP2011050377A (ja) 新規hla−drb1遺伝子およびその用途