FR3106596B1 - Procede de genotypage hla simple et rapide - Google Patents

Procede de genotypage hla simple et rapide Download PDF

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Publication number
FR3106596B1
FR3106596B1 FR2000775A FR2000775A FR3106596B1 FR 3106596 B1 FR3106596 B1 FR 3106596B1 FR 2000775 A FR2000775 A FR 2000775A FR 2000775 A FR2000775 A FR 2000775A FR 3106596 B1 FR3106596 B1 FR 3106596B1
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hla
genotyping
rapid
hla genotyping
genotyping process
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FR2000775A
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FR3106596A1 (fr
Inventor
Pascal Herve
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Bionobis SARL
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Bionobis SARL
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Priority to FR2000775A priority Critical patent/FR3106596B1/fr
Priority to EP21707765.0A priority patent/EP4097254A1/fr
Priority to PCT/FR2021/050145 priority patent/WO2021152257A1/fr
Publication of FR3106596A1 publication Critical patent/FR3106596A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L’invention a trait à un procédé de génotypage du système HLA permettant de traiter simultanément un grand nombre d’échantillons d’ADN. Plus particulièrement, elle concerne un procédé de génotypage HLA comportant une première étape d’amplification par PCR indexée en système microfluidique puis une seconde étape de séquençage à haut débit, ce procédé est simple, rapide et moins coûteux que les procédés de l’état de la technique. L’invention est plus particulièrement appliquée au génotypage avec une résolution G groupe du locus HLA-DRB, un gène majeur dans la détermination du typage HLA.
FR2000775A 2020-01-27 2020-01-27 Procede de genotypage hla simple et rapide Active FR3106596B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR2000775A FR3106596B1 (fr) 2020-01-27 2020-01-27 Procede de genotypage hla simple et rapide
EP21707765.0A EP4097254A1 (fr) 2020-01-27 2021-01-26 Procede de genotypage hla simple et rapide
PCT/FR2021/050145 WO2021152257A1 (fr) 2020-01-27 2021-01-26 Procede de genotypage hla simple et rapide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2000775 2020-01-27
FR2000775A FR3106596B1 (fr) 2020-01-27 2020-01-27 Procede de genotypage hla simple et rapide

Publications (2)

Publication Number Publication Date
FR3106596A1 FR3106596A1 (fr) 2021-07-30
FR3106596B1 true FR3106596B1 (fr) 2022-12-02

Family

ID=71111503

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2000775A Active FR3106596B1 (fr) 2020-01-27 2020-01-27 Procede de genotypage hla simple et rapide

Country Status (3)

Country Link
EP (1) EP4097254A1 (fr)
FR (1) FR3106596B1 (fr)
WO (1) WO2021152257A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117265091B (zh) * 2023-10-31 2024-06-14 江苏伟禾生物科技有限公司 一种用于hla-drb3/4/5基因分型的引物组、试剂盒及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2257139B1 (es) * 2003-12-17 2007-07-16 Universitat Autonoma De Barcelona Metodo y kit para genotipificacion de la hla-drb basados en la pcr en tiempo real.
ES2276630B1 (es) 2005-12-13 2008-06-16 Universitat Autonoma De Barcelona Metodo y kit para genotipificacion de hla-b basados en la pcr en tiempo real.
WO2009049889A1 (fr) * 2007-10-16 2009-04-23 Roche Diagnostics Gmbh Génotypage hla à haute résolution et haut débit par séquençage clonal
US9957564B2 (en) 2010-06-30 2018-05-01 Bgi Genomics Co., Ltd. Application of a PCR sequencing method, based on DNA barcoding technique and DNA incomplete shearing strategy, in HLA genotyping

Also Published As

Publication number Publication date
EP4097254A1 (fr) 2022-12-07
FR3106596A1 (fr) 2021-07-30
WO2021152257A1 (fr) 2021-08-05

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