CA2803940C - Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping - Google Patents

Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping Download PDF

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CA2803940C
CA2803940C CA2803940A CA2803940A CA2803940C CA 2803940 C CA2803940 C CA 2803940C CA 2803940 A CA2803940 A CA 2803940A CA 2803940 A CA2803940 A CA 2803940A CA 2803940 C CA2803940 C CA 2803940C
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hla
pcr
dqb1
dna
sequencing
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CA2803940A1 (en
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Jian Li
Shiping Chen
Xiandong Zhang
Ying Liu
Caifen Zhang
Tao Liu
Meiru Zhao
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BGI Genomics Co Ltd
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Priority claimed from CN201010213717.6A external-priority patent/CN101921840B/zh
Priority claimed from CN 201010213721 external-priority patent/CN101921842B/zh
Priority claimed from CN201010213719.5A external-priority patent/CN101921841B/zh
Priority claimed from PCT/CN2010/002149 external-priority patent/WO2012083505A1/zh
Priority claimed from PCT/CN2010/002150 external-priority patent/WO2012083506A1/zh
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
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    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CA2803940A 2010-06-30 2011-06-30 Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping Active CA2803940C (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
CN201010213719.5 2010-06-30
CN201010213717.6A CN101921840B (zh) 2010-06-30 2010-06-30 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
CN 201010213721 CN101921842B (zh) 2010-06-30 2010-06-30 Hla-a,b基因分型用pcr引物及其使用方法
CN201010213717.6 2010-06-30
CN201010213721.2 2010-06-30
CN201010213719.5A CN101921841B (zh) 2010-06-30 2010-06-30 基于Illumina GA测序技术的HLA基因高分辨率分型方法
CNPCT/CN2010/002149 2010-12-24
PCT/CN2010/002149 WO2012083505A1 (zh) 2010-12-24 2010-12-24 Hla-c基因分型的方法及其相关引物
PCT/CN2010/002150 WO2012083506A1 (zh) 2010-12-24 2010-12-24 Hla-dqb1基因分型的方法及其相关引物
CNPCT/CN2010/002150 2010-12-24
PCT/CN2011/076688 WO2012000445A1 (zh) 2010-06-30 2011-06-30 新的的pcr测序方法及其在hla基因分型中的应用

Publications (2)

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CA2803940A1 CA2803940A1 (en) 2012-01-05
CA2803940C true CA2803940C (en) 2019-07-02

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US (1) US9957564B2 (https=)
EP (1) EP2599877B1 (https=)
JP (1) JP5968879B2 (https=)
KR (1) KR101709826B1 (https=)
AU (1) AU2011274090B2 (https=)
BR (1) BR112012032586B1 (https=)
CA (1) CA2803940C (https=)
DK (1) DK2599877T3 (https=)
MY (1) MY173793A (https=)
RU (1) RU2587606C2 (https=)
SG (1) SG186876A1 (https=)
WO (1) WO2012000445A1 (https=)

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Publication number Publication date
DK2599877T3 (da) 2017-11-20
CA2803940A1 (en) 2012-01-05
AU2011274090A1 (en) 2013-02-07
RU2013103795A (ru) 2014-08-20
SG186876A1 (en) 2013-02-28
MY173793A (en) 2020-02-24
EP2599877A4 (en) 2014-01-01
AU2011274090B2 (en) 2015-04-09
KR20130038353A (ko) 2013-04-17
KR101709826B1 (ko) 2017-02-24
US20130237432A1 (en) 2013-09-12
BR112012032586B1 (pt) 2021-08-17
BR112012032586A2 (pt) 2019-07-30
US9957564B2 (en) 2018-05-01
JP5968879B2 (ja) 2016-08-10
WO2012000445A1 (zh) 2012-01-05
JP2013529472A (ja) 2013-07-22
EP2599877A1 (en) 2013-06-05
EP2599877B1 (en) 2017-09-27
RU2587606C2 (ru) 2016-06-20

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