BR112012032586A2 - novo método de sequenciamento de pcr e uso do mesmo na genotipagem hla. - Google Patents
novo método de sequenciamento de pcr e uso do mesmo na genotipagem hla.Info
- Publication number
- BR112012032586A2 BR112012032586A2 BR112012032586A BR112012032586A BR112012032586A2 BR 112012032586 A2 BR112012032586 A2 BR 112012032586A2 BR 112012032586 A BR112012032586 A BR 112012032586A BR 112012032586 A BR112012032586 A BR 112012032586A BR 112012032586 A2 BR112012032586 A2 BR 112012032586A2
- Authority
- BR
- Brazil
- Prior art keywords
- hla
- sequencing
- sequencing method
- pcr
- primary
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Abstract
novo método de sequencimanto de pcr e uso do mesmo na genotipagem hla - a invenção proporciona um método de sequencimento de pcr, onde a combinação de índices primários, uma estratégia de cisalhamento de dna incompleto e uma técnica de sequenciamento de segunda geração (técnica de sequenciamento de extremidade em pares) pode fazer com que o comprimento dos produtos de pcr que podem ser sequenciados por um sequenciador maiores do que o comprimento do sequenciamento máximo do sequenciador enquanto faz o uso completo das características da técnica de sequenciamento de segunda geração tal como um alto rendimento e baixo custo, desse modo, ampliando muito seu escopo aplicável. além disso, a presente invenção também proporciona índices primários para o método de sequencimanto de pcr e o uso do método em genotipagem, particularmente na analisa de hla, e também proporciona os pcr primários usados, particularmente os pcr primários para os genes hla-a,b, hla-c e hla-dqb1.
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010213721 CN101921842B (zh) | 2010-06-30 | 2010-06-30 | Hla-a,b基因分型用pcr引物及其使用方法 |
| CN201010213717.6A CN101921840B (zh) | 2010-06-30 | 2010-06-30 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
| CN201010213719.5 | 2010-06-30 | ||
| CN201010213719.5A CN101921841B (zh) | 2010-06-30 | 2010-06-30 | 基于Illumina GA测序技术的HLA基因高分辨率分型方法 |
| CN201010213717.6 | 2010-06-30 | ||
| CN201010213721.2 | 2010-06-30 | ||
| CNPCT/CN2010/002150 | 2010-12-24 | ||
| PCT/CN2010/002150 WO2012083506A1 (zh) | 2010-12-24 | 2010-12-24 | Hla-dqb1基因分型的方法及其相关引物 |
| CNPCT/CN2010/002149 | 2010-12-24 | ||
| PCT/CN2010/002149 WO2012083505A1 (zh) | 2010-12-24 | 2010-12-24 | Hla-c基因分型的方法及其相关引物 |
| PCT/CN2011/076688 WO2012000445A1 (zh) | 2010-06-30 | 2011-06-30 | 新的的pcr测序方法及其在hla基因分型中的应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112012032586A2 true BR112012032586A2 (pt) | 2019-07-30 |
| BR112012032586B1 BR112012032586B1 (pt) | 2021-08-17 |
Family
ID=45401405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012032586-8A BR112012032586B1 (pt) | 2010-06-30 | 2011-06-30 | Métodos para determinar a sequência nucleotídica de um ácido nucléico de interesse e para determinar o genótipo hla em uma amostra |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9957564B2 (pt) |
| EP (1) | EP2599877B1 (pt) |
| JP (1) | JP5968879B2 (pt) |
| KR (1) | KR101709826B1 (pt) |
| AU (1) | AU2011274090B2 (pt) |
| BR (1) | BR112012032586B1 (pt) |
| CA (1) | CA2803940C (pt) |
| DK (1) | DK2599877T3 (pt) |
| MY (1) | MY173793A (pt) |
| RU (1) | RU2587606C2 (pt) |
| SG (1) | SG186876A1 (pt) |
| WO (1) | WO2012000445A1 (pt) |
Families Citing this family (30)
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| WO2014171898A2 (en) * | 2013-04-17 | 2014-10-23 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| JP6308724B2 (ja) * | 2013-05-09 | 2018-04-11 | ジェノダイブファーマ株式会社 | Ｈｌａ遺伝子のマルチプレックスｄｎａタイピング方法及びキット |
| EP3038649B1 (en) * | 2013-08-26 | 2019-09-25 | The Translational Genomics Research Institute | Single molecule-overlapping read analysis for minor variant mutation detection in pathogen samples |
| GB201409282D0 (en) | 2014-05-23 | 2014-07-09 | Univ Sydney Tech | Sequencing process |
| US10648031B2 (en) * | 2014-09-05 | 2020-05-12 | Qiagen Gmbh | Preparation of adapter-ligated amplicons |
| CN105400864B (zh) * | 2014-09-12 | 2020-04-14 | 深圳华大基因股份有限公司 | 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途 |
| CN107075513B (zh) * | 2014-09-12 | 2020-11-03 | 深圳华大智造科技有限公司 | 分离的寡核苷酸及其在核酸测序中的用途 |
| US10233490B2 (en) | 2014-11-21 | 2019-03-19 | Metabiotech Corporation | Methods for assembling and reading nucleic acid sequences from mixed populations |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| CA2971589C (en) | 2014-12-18 | 2021-09-28 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| WO2016124069A1 (zh) * | 2015-02-04 | 2016-08-11 | 深圳华大基因研究院 | 一种构建长片段测序文库的方法 |
| KR101651817B1 (ko) * | 2015-10-28 | 2016-08-29 | 대한민국 | Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트 |
| EP3420104A1 (en) * | 2016-02-23 | 2019-01-02 | Novozymes A/S | Improved next-generation sequencing |
| EP3459115A4 (en) | 2016-05-16 | 2020-04-08 | Agilome, Inc. | GRAPHEN-FET DEVICES, SYSTEMS AND METHODS FOR USE THEREOF FOR SEQUENCING NUCLEIC ACIDS |
| WO2018081113A1 (en) | 2016-10-24 | 2018-05-03 | Sawaya Sterling | Concealing information present within nucleic acids |
| US11441192B2 (en) * | 2016-11-15 | 2022-09-13 | Quest Diagnostics Investments Llc | Methods for detecting DNA mutations using mitra tip extraction |
| WO2018093723A1 (en) | 2016-11-15 | 2018-05-24 | Quest Diagnostics Investments Llc | Methods for detecting cystic fibrosis mutations using mitra tip extraction |
| EP3626835A1 (en) | 2018-09-18 | 2020-03-25 | Sistemas Genómicos, S.L. | Method for genotypically identifying both alleles of at least one locus of a subject's hla gene |
| RU2703394C1 (ru) * | 2018-10-24 | 2019-10-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Способ выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней |
| RU2703401C1 (ru) * | 2018-10-24 | 2019-10-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Тест-система для выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней |
| FR3100820B1 (fr) | 2019-09-12 | 2024-06-28 | Bionobis | Procédé de génotypage adapté au traitement d’un grand nombre d’échantillons, notamment en cas de polymorphisme élevé |
| FR3106596B1 (fr) | 2020-01-27 | 2022-12-02 | Bionobis | Procede de genotypage hla simple et rapide |
| CN111235266B (zh) * | 2020-03-10 | 2023-12-01 | 广州医科大学附属第二医院 | Hla亚型检测试剂盒及其应用 |
| FR3110178B1 (fr) * | 2020-05-18 | 2022-05-27 | Bionobis | Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients |
| CN112553313A (zh) * | 2020-12-30 | 2021-03-26 | 武汉康圣达医学检验所有限公司 | 用于Sanger法的测序反应产物的变性方法 |
| KR102507110B1 (ko) * | 2022-02-15 | 2023-03-07 | 주식회사 네오젠티씨 | 주조직 적합성 복합체의 타입들을 분석하기 위한 방법 및 장치 |
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| JP2011500041A (ja) | 2007-10-16 | 2011-01-06 | エフ．ホフマン−ラ ロシュ アーゲー | クローナルシークエンシングによる高分解能かつ高効率のｈｌａ遺伝子型決定法 |
| RU2393231C1 (ru) * | 2008-12-29 | 2010-06-27 | Федеральное государственное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб") | Способ определения генетического родства штаммов холерных вибрионов методом секвенирования генов, фланкирующих кластер генов биосинтеза о-антигена |
| CN101921840B (zh) * | 2010-06-30 | 2014-06-25 | 深圳华大基因科技有限公司 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
| CN101921842B (zh) | 2010-06-30 | 2013-08-07 | 深圳华大基因科技有限公司 | Hla-a,b基因分型用pcr引物及其使用方法 |
| CN101921841B (zh) * | 2010-06-30 | 2014-03-12 | 深圳华大基因科技有限公司 | 基于Illumina GA测序技术的HLA基因高分辨率分型方法 |
-
2011
- 2011-06-30 AU AU2011274090A patent/AU2011274090B2/en active Active
- 2011-06-30 MY MYPI2012005591A patent/MY173793A/en unknown
- 2011-06-30 CA CA2803940A patent/CA2803940C/en active Active
- 2011-06-30 WO PCT/CN2011/076688 patent/WO2012000445A1/zh active Application Filing
- 2011-06-30 SG SG2012096616A patent/SG186876A1/en unknown
- 2011-06-30 JP JP2013516983A patent/JP5968879B2/ja active Active
- 2011-06-30 US US13/807,660 patent/US9957564B2/en active Active
- 2011-06-30 DK DK11800190.8T patent/DK2599877T3/da active
- 2011-06-30 KR KR1020137002332A patent/KR101709826B1/ko active IP Right Grant
- 2011-06-30 EP EP11800190.8A patent/EP2599877B1/en active Active
- 2011-06-30 RU RU2013103795/10A patent/RU2587606C2/ru active IP Right Revival
- 2011-06-30 BR BR112012032586-8A patent/BR112012032586B1/pt active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| SG186876A1 (en) | 2013-02-28 |
| AU2011274090B2 (en) | 2015-04-09 |
| JP5968879B2 (ja) | 2016-08-10 |
| JP2013529472A (ja) | 2013-07-22 |
| KR20130038353A (ko) | 2013-04-17 |
| US20130237432A1 (en) | 2013-09-12 |
| MY173793A (en) | 2020-02-24 |
| AU2011274090A1 (en) | 2013-02-07 |
| BR112012032586B1 (pt) | 2021-08-17 |
| EP2599877B1 (en) | 2017-09-27 |
| US9957564B2 (en) | 2018-05-01 |
| CA2803940C (en) | 2019-07-02 |
| EP2599877A1 (en) | 2013-06-05 |
| EP2599877A4 (en) | 2014-01-01 |
| RU2587606C2 (ru) | 2016-06-20 |
| RU2013103795A (ru) | 2014-08-20 |
| DK2599877T3 (da) | 2017-11-20 |
| WO2012000445A1 (zh) | 2012-01-05 |
| KR101709826B1 (ko) | 2017-02-24 |
| CA2803940A1 (en) | 2012-01-05 |
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