SG186876A1 - New pcr sequencing method and use thereof in hla genotyping - Google Patents

New pcr sequencing method and use thereof in hla genotyping Download PDF

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Publication number
SG186876A1
SG186876A1 SG2012096616A SG2012096616A SG186876A1 SG 186876 A1 SG186876 A1 SG 186876A1 SG 2012096616 A SG2012096616 A SG 2012096616A SG 2012096616 A SG2012096616 A SG 2012096616A SG 186876 A1 SG186876 A1 SG 186876A1
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SG
Singapore
Prior art keywords
hla
pcr
seq
sequencing
pcr primers
Prior art date
Application number
SG2012096616A
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English (en)
Inventor
Jian Li
Shiping Chen
Xiandong Zhang
Ying Liu
Caifen Zhang
Tao Liu
Meiru Zhao
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Bgi Shenzhen Co Ltd
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Publication date
Priority claimed from CN 201010213721 external-priority patent/CN101921842B/zh
Priority claimed from CN201010213717.6A external-priority patent/CN101921840B/zh
Priority claimed from CN201010213719.5A external-priority patent/CN101921841B/zh
Priority claimed from PCT/CN2010/002149 external-priority patent/WO2012083505A1/zh
Priority claimed from PCT/CN2010/002150 external-priority patent/WO2012083506A1/zh
Application filed by Bgi Shenzhen Co Ltd filed Critical Bgi Shenzhen Co Ltd
Publication of SG186876A1 publication Critical patent/SG186876A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
SG2012096616A 2010-06-30 2011-06-30 New pcr sequencing method and use thereof in hla genotyping SG186876A1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN 201010213721 CN101921842B (zh) 2010-06-30 2010-06-30 Hla-a,b基因分型用pcr引物及其使用方法
CN201010213717.6A CN101921840B (zh) 2010-06-30 2010-06-30 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
CN201010213719.5A CN101921841B (zh) 2010-06-30 2010-06-30 基于Illumina GA测序技术的HLA基因高分辨率分型方法
PCT/CN2010/002149 WO2012083505A1 (zh) 2010-12-24 2010-12-24 Hla-c基因分型的方法及其相关引物
PCT/CN2010/002150 WO2012083506A1 (zh) 2010-12-24 2010-12-24 Hla-dqb1基因分型的方法及其相关引物
PCT/CN2011/076688 WO2012000445A1 (zh) 2010-06-30 2011-06-30 新的的pcr测序方法及其在hla基因分型中的应用

Publications (1)

Publication Number Publication Date
SG186876A1 true SG186876A1 (en) 2013-02-28

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SG2012096616A SG186876A1 (en) 2010-06-30 2011-06-30 New pcr sequencing method and use thereof in hla genotyping

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US (1) US9957564B2 (enExample)
EP (1) EP2599877B1 (enExample)
JP (1) JP5968879B2 (enExample)
KR (1) KR101709826B1 (enExample)
AU (1) AU2011274090B2 (enExample)
BR (1) BR112012032586B1 (enExample)
CA (1) CA2803940C (enExample)
DK (1) DK2599877T3 (enExample)
MY (1) MY173793A (enExample)
RU (1) RU2587606C2 (enExample)
SG (1) SG186876A1 (enExample)
WO (1) WO2012000445A1 (enExample)

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Also Published As

Publication number Publication date
EP2599877B1 (en) 2017-09-27
WO2012000445A1 (zh) 2012-01-05
DK2599877T3 (da) 2017-11-20
JP2013529472A (ja) 2013-07-22
CA2803940C (en) 2019-07-02
AU2011274090B2 (en) 2015-04-09
EP2599877A1 (en) 2013-06-05
AU2011274090A1 (en) 2013-02-07
BR112012032586B1 (pt) 2021-08-17
RU2013103795A (ru) 2014-08-20
JP5968879B2 (ja) 2016-08-10
RU2587606C2 (ru) 2016-06-20
MY173793A (en) 2020-02-24
BR112012032586A2 (pt) 2019-07-30
EP2599877A4 (en) 2014-01-01
CA2803940A1 (en) 2012-01-05
KR101709826B1 (ko) 2017-02-24
US20130237432A1 (en) 2013-09-12
US9957564B2 (en) 2018-05-01
KR20130038353A (ko) 2013-04-17

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