SG147468A1 - High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences - Google Patents

High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

Info

Publication number
SG147468A1
SG147468A1 SG200807906-3A SG2008079063A SG147468A1 SG 147468 A1 SG147468 A1 SG 147468A1 SG 2008079063 A SG2008079063 A SG 2008079063A SG 147468 A1 SG147468 A1 SG 147468A1
Authority
SG
Singapore
Prior art keywords
matrix attachment
expression
high efficiency
mammalian cells
gene transfer
Prior art date
Application number
SG200807906-3A
Other languages
English (en)
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Publication of SG147468A1 publication Critical patent/SG147468A1/en

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Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SG200807906-3A 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences SG147468A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
EP04002722 2004-02-06

Publications (1)

Publication Number Publication Date
SG147468A1 true SG147468A1 (en) 2008-11-28

Family

ID=34524793

Family Applications (1)

Application Number Title Priority Date Filing Date
SG200807906-3A SG147468A1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

Country Status (16)

Country Link
US (3) US8252917B2 (fr)
EP (5) EP2298898B1 (fr)
JP (1) JP5396653B2 (fr)
KR (4) KR101222025B1 (fr)
CN (1) CN1863913B (fr)
AT (1) ATE551420T1 (fr)
AU (4) AU2004284220B2 (fr)
CA (1) CA2535836C (fr)
HK (4) HK1151824A1 (fr)
IL (2) IL175142A0 (fr)
IN (2) IN2014DN02483A (fr)
PL (4) PL2298898T3 (fr)
RU (1) RU2375450C2 (fr)
SG (1) SG147468A1 (fr)
WO (1) WO2005040377A2 (fr)
ZA (1) ZA200604032B (fr)

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CN104974933B (zh) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法
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HK1151824A1 (en) 2012-02-10
AU2004284220B2 (en) 2010-10-14
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AU2010236072A1 (en) 2010-11-18
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EP1959011B1 (fr) 2012-03-28
EP1959011A3 (fr) 2008-08-27
WO2005040377A2 (fr) 2005-05-06
IL175142A0 (en) 2006-09-05
CN1863913B (zh) 2011-01-12
IL198863A (en) 2011-09-27
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US20180187229A1 (en) 2018-07-05
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