IN2014DN02484A - - Google Patents

Info

Publication number
IN2014DN02484A
IN2014DN02484A IN2484DEN2014A IN2014DN02484A IN 2014DN02484 A IN2014DN02484 A IN 2014DN02484A IN 2484DEN2014 A IN2484DEN2014 A IN 2484DEN2014A IN 2014DN02484 A IN2014DN02484 A IN 2014DN02484A
Authority
IN
India
Prior art keywords
protein production
sequences
activity
mar
increasing
Prior art date
Application number
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Publication of IN2014DN02484A publication Critical patent/IN2014DN02484A/en

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Evolutionary Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.
IN2484DEN2014 2003-10-24 2004-10-22 IN2014DN02484A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
EP04002722 2004-02-06
PCT/EP2004/011974 WO2005040377A2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

Publications (1)

Publication Number Publication Date
IN2014DN02484A true IN2014DN02484A (en) 2015-05-15

Family

ID=34524793

Family Applications (2)

Application Number Title Priority Date Filing Date
IN2484DEN2014 IN2014DN02484A (en) 2003-10-24 2004-10-22
IN2483DEN2014 IN2014DN02483A (en) 2003-10-24 2004-10-22

Family Applications After (1)

Application Number Title Priority Date Filing Date
IN2483DEN2014 IN2014DN02483A (en) 2003-10-24 2004-10-22

Country Status (16)

Country Link
US (3) US8252917B2 (en)
EP (5) EP1675952A2 (en)
JP (1) JP5396653B2 (en)
KR (4) KR101269656B1 (en)
CN (1) CN1863913B (en)
AT (1) ATE551420T1 (en)
AU (4) AU2004284220B2 (en)
CA (1) CA2535836C (en)
HK (4) HK1151824A1 (en)
IL (2) IL175142A0 (en)
IN (2) IN2014DN02484A (en)
PL (4) PL2292754T3 (en)
RU (1) RU2375450C2 (en)
SG (1) SG147468A1 (en)
WO (1) WO2005040377A2 (en)
ZA (1) ZA200604032B (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1572994B1 (en) 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
KR101269656B1 (en) * 2003-10-24 2013-05-30 셀렉시스 에스. 에이. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
DE102004043207C5 (en) * 2004-09-03 2010-09-09 Südzucker AG Mannheim/Ochsenfurt Root and xylem parenchyma specific promoter
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
CA2581422C (en) 2004-11-08 2012-05-22 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (en) 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
CA2658775A1 (en) * 2006-08-23 2008-02-28 Selexis S.A. Matrix attachment regions (mars) for increasing transcription and uses thereof
EP1975228A1 (en) 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
EP3508505A1 (en) 2007-12-24 2019-07-10 ID Biomedical Corporation of Quebec Recombinant rsv antigens
CN101580842B (en) * 2008-05-15 2011-02-09 中国农业科学院生物技术研究所 DNA molecule capable of reinforcing gene expression and use thereof
WO2010081890A1 (en) 2009-01-19 2010-07-22 Innate Pharma Anti-kir3d antibodies
PE20121541A1 (en) 2009-06-24 2012-12-21 Glaxosmithkline Biolog Sa RECOMBINANT RESPIRATORY SYNCIZAL VIRUS ANTIGENS
US8889146B2 (en) 2009-06-24 2014-11-18 Glaxosmithkline Biologicals, Sa Vaccine
SG178026A1 (en) 2009-07-15 2012-03-29 Novartis Ag Rsv f protein compositions and methods for making same
KR20120099376A (en) 2009-09-18 2012-09-10 셀렉시스 에스. 에이. Products and methods for enhanced transgene expression and processing
WO2011070405A1 (en) * 2009-12-07 2011-06-16 Avesthagen Limited An expression vector carrying scaffold/matrix attachment region(s)
WO2012019630A1 (en) 2010-08-13 2012-02-16 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
WO2012139195A1 (en) 2011-04-13 2012-10-18 National Research Council Of Canada EXPRESSION SYSTEM WITH SAR ELEMENT FROM IFNα2
WO2013120499A1 (en) * 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
WO2013120500A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen
WO2013120497A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein
WO2013120498A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen
JP6185553B2 (en) 2012-03-27 2017-08-23 キュアバック アーゲー Artificial nucleic acid molecules for improving protein or peptide expression
DK2951309T3 (en) 2013-02-01 2019-04-23 Selexis Sa INCREASED TRANSGEN EXPRESSION AND PROCESSING
CN103667440A (en) * 2013-09-05 2014-03-26 谢小冬 Application of SNP loci of GATA4 genes
US9982027B2 (en) 2013-10-22 2018-05-29 Lubris Llc Control of rheological properties of mixed hyaluronate/lubricin solutions
CN109576265B (en) * 2014-04-01 2020-08-28 三生国健药业（上海）股份有限公司 Animal cell expression vector containing MAR core fragment
CN104974933B (en) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 A kind of extensive continuous several times, which suspend, turns the apparatus and method of expression recombinant protein wink
WO2017202776A1 (en) * 2016-05-23 2017-11-30 Luxembourg Institute Of Health (Lih) Multifunctional heteromultimeric constructs.
JP2022509451A (en) 2018-10-24 2022-01-20 セレキシス エスエー Expression system, recombinant cells and their use
JP2022511077A (en) 2018-12-07 2022-01-28 セルテオン コーポレイション Use in matrix attachment regions and promotion of gene expression
EP4110798A1 (en) 2020-02-24 2023-01-04 Novartis AG Purification of recombinantly produced polypeptides

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1077994A (en) * 1963-04-18 1967-08-02 Kobe Steel Ltd Process for producing cold-forged products from tempered steel wire
DE2605560C3 (en) 1976-02-12 1979-08-30 Siemens Ag, 1000 Berlin Und 8000 Muenchen Digestion process for biomaterials
EP0113551B1 (en) 1982-12-23 1988-04-27 Britax Weathershields Limited Vehicle sliding roof
DE3751873T2 (en) 1986-04-09 1997-02-13 Genzyme Corp Genetically transformed animals that secrete a desired protein in milk
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
GB8925590D0 (en) 1989-11-13 1990-01-04 Central Blood Lab Authority Monoclonal antibodies
US5733761A (en) 1991-11-05 1998-03-31 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
PT663921E (en) 1992-10-05 2002-10-31 Univ North Carolina State METHOD FOR INCREASING EXPRESSION AND REDUCING THE VARIABILITY OF EXPRESSION OF STRANGE GENES IN VEGETABLE CELLS
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5907078A (en) 1994-12-09 1999-05-25 Greenberg; Norman M. Transgenic mouse model for prostate cancer
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
DE19541450C2 (en) * 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Gene construct and its use
US6596514B2 (en) 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
AU2064297A (en) 1996-02-29 1997-09-16 Immusol, Inc Hepatitis c virus ribozymes
CA2256484A1 (en) 1996-06-06 1997-12-11 Novartis Ag Vectors comprising sar elements
CA2689842A1 (en) 1996-12-02 1998-06-11 Genaera Corporation Il-9 receptor variants, useful in treating and diagnosing atopic allergies including asthma and related disorders
US6245974B1 (en) 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
EP1029048A2 (en) 1997-11-05 2000-08-23 Baylor College Of Medicine Sequences for targeting metastatic cells
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6004284A (en) 1998-06-04 1999-12-21 Alcon Laboratories, Inc. Surgical handpiece
CN100365127C (en) 1998-07-21 2008-01-30 密理博公司 Polynucleotide comprising ubiquitous chromatin opening element (UCDE)
US6338066B1 (en) * 1998-09-25 2002-01-08 International Business Machines Corporation Surfaid predictor: web-based system for predicting surfer behavior
JP4527882B2 (en) 1998-10-07 2010-08-18 アドビ・システムズ・インコーポレーテッド Method and system for distributing access to data items
DE19848017A1 (en) 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomally replicating vectors, useful in gene therapy, contain origins of replication and scaffold-matrix attached region sequences but no viral coding sequences
JP4544748B2 (en) * 1998-12-01 2010-09-15 ダウ・アグロサイエンス・エル・エル・シー Artificial matrix attachment region to increase the expression of genes introduced into plant cells
US6225113B1 (en) 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
EP1181359A1 (en) 1999-05-28 2002-02-27 Sangamo Biosciences Inc. Gene switches
US20050129669A1 (en) 1999-06-21 2005-06-16 Transkaryotic Therapies, Inc., A Massachusetts Corporation DNA construct for effecting homologous recombination and uses thereof
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
JP3507858B2 (en) 1999-11-19 2004-03-15 奈良先端科学技術大学院大学長 DNA fragments that stabilize the expression of foreign genes in plants, gene introduction methods for stably expressing foreign genes in plants, transformed plants that stably express foreign genes
WO2001040488A1 (en) 1999-11-30 2001-06-07 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6864085B2 (en) 1999-12-14 2005-03-08 Novartis Ag Bovine immunodeficiency virus (BIV) based vectors
US20020127199A1 (en) 2000-02-03 2002-09-12 Tang Y. Tom Novel nucleic acids and polypeptides
US6821775B1 (en) 2000-02-11 2004-11-23 Genvec, Inc. Viral vector encoding pigment epithelium-derived factor
DE10006886A1 (en) 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Non-human helper dependent virus vector
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6747189B1 (en) 2000-03-21 2004-06-08 Dekalb Genetics Corporation Maize glycine rich protein promoter compositions and methods for use thereof
US6730826B2 (en) 2000-03-22 2004-05-04 University Of Kentucky Research Foundation Plant trichome gland specific promoter sequence
EP1294406A2 (en) 2000-06-26 2003-03-26 Universite De Geneve Modulation of chromosome function by chromatin remodeling agents
US20030157715A1 (en) 2000-06-26 2003-08-21 Ulrich Laemmli Modulation of chromosome function by chromatin remodeling agents
CA2416877C (en) 2000-07-27 2013-03-12 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for producing recombinant proteins, cells and organs
KR100408844B1 (en) 2000-07-29 2003-12-06 한국산업기술평가원 Expression vector using for animal cell
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
AU9379801A (en) 2000-09-09 2002-03-22 Basf Plant Science Gmbh Modified tet-inducible system for regulation of gene expression in plants
GB0022995D0 (en) 2000-09-20 2000-11-01 Cobra Therapeutics Ltd Polynucleotide
US6919204B2 (en) 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
WO2002046208A2 (en) 2000-11-01 2002-06-13 Elusys Therapeutics, Inc. Method of producing biospecific molecules by protein trans-splicing
DE10061150A1 (en) 2000-12-08 2002-06-13 Icon Genetics Ag Methods and vectors for the production of transgenic plants
US7259010B2 (en) 2000-12-15 2007-08-21 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region of interferon beta
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
DK1395669T3 (en) * 2001-01-26 2009-11-16 Selexis Sa Matrix binding regions and methods for using them
DE10109780A1 (en) 2001-02-28 2002-09-05 Cardion Ag Episomal vectors containing matrix attachment regions for cell-specific gene expression
WO2002072138A1 (en) 2001-03-08 2002-09-19 Hyseq, Inc. Methods and materials relating to fibulin-like polypeptides and polynucleotides
JP4817514B2 (en) 2001-03-09 2011-11-16 協和発酵キリン株式会社 Novel animal cell vectors and uses thereof
US20040076954A1 (en) 2001-03-12 2004-04-22 Irm, Llc Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
US7070993B2 (en) 2001-03-13 2006-07-04 Novartis Ag Lentiviral packaging constructs
AU2002255995A1 (en) 2001-03-30 2002-10-15 Avigenics, Inc. Avian lysozyme promoter
DE10129010A1 (en) 2001-06-13 2002-12-19 Icon Genetics Ag Producing a transgenic plant or plant cell expressing a transgene under control of native transcription and translation elements already present in the genome is useful to give stable expression of transgenes
US20030032597A1 (en) 2001-07-31 2003-02-13 Sebestyen Magdolna G. Targeting nucleic acids to a cellular nucleus
KR100464962B1 (en) * 2001-09-14 2005-01-05 삼화강봉주식회사 Quenched & tempered steel wire with superior characteristics of cold forging
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
WO2003024199A2 (en) 2001-09-21 2003-03-27 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US7138278B2 (en) 2001-11-20 2006-11-21 Monsanto Technology, L.L.C. Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof
FR2832423B1 (en) 2001-11-22 2004-10-08 Vivalis EXOGENOUS PROTEIN EXPRESSION SYSTEM IN AN AVIAN SYSTEM
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
CA2427190A1 (en) 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
US20030224477A1 (en) 2002-05-31 2003-12-04 Heartlein Michael W. Optimized promoter constructs
EP1513929A4 (en) 2002-06-18 2006-04-19 Zymogenetics Inc Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter
US20040016015A1 (en) 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US6933136B2 (en) 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins
CN1263860C (en) 2002-09-30 2006-07-12 华南农业大学 Construction method of multigene carrier and its application
WO2004053106A2 (en) 2002-12-05 2004-06-24 Regulome Corporation Profiled regulatory sites useful for gene control
AU2003283174A1 (en) 2002-12-11 2004-06-30 Cytos Biotechnology Ag Method for protein production
AU2002953381A0 (en) 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1445320A1 (en) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
WO2004094640A1 (en) 2003-04-15 2004-11-04 University Of Georgia Research Foundation, Inc. Avian transgenesis using a chicken ovalbumin gene region
CA2527694C (en) 2003-05-30 2015-07-14 Hendricus Renerus Jacobus Mattheus Hoogenboom Fab library for the preparation of anti vegf and anti rabies virus fabs
EP1644508A1 (en) * 2003-07-11 2006-04-12 Cytos Biotechnology AG Gene expression system
RU2373285C2 (en) 2003-08-27 2009-11-20 Орф Лифтаэкни Хф. Method of increasing accumulation of heterologous polypeptides in plant seeds through direct suppression of endogeneous reserve proteins
EP1675956B1 (en) 2003-10-21 2010-10-13 Merck Serono SA Minimal dna sequence acting as a chromatin insulator and its use in protein expression
KR101269656B1 (en) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
CA2658775A1 (en) 2006-08-23 2008-02-28 Selexis S.A. Matrix attachment regions (mars) for increasing transcription and uses thereof
US20090018031A1 (en) * 2006-12-07 2009-01-15 Switchgear Genomics Transcriptional regulatory elements of biological pathways tools, and methods

Also Published As

Publication number Publication date
US20180187229A1 (en) 2018-07-05
KR101269656B1 (en) 2013-05-30
IL198863A (en) 2011-09-27
AU2010236093B2 (en) 2011-10-13
ATE551420T1 (en) 2012-04-15
KR20110112884A (en) 2011-10-13
EP2292754A1 (en) 2011-03-09
AU2010236072B2 (en) 2011-09-01
AU2004284220A1 (en) 2005-05-06
EP1959011A3 (en) 2008-08-27
EP2298898B1 (en) 2012-12-26
EP2287302B1 (en) 2012-12-26
EP1959011B1 (en) 2012-03-28
HK1120549A1 (en) 2009-04-03
WO2005040377A3 (en) 2005-09-15
JP2007508831A (en) 2007-04-12
PL1959011T3 (en) 2012-07-31
US8252917B2 (en) 2012-08-28
US9879297B2 (en) 2018-01-30
IL175142A0 (en) 2006-09-05
EP1959011A2 (en) 2008-08-20
KR20110112882A (en) 2011-10-13
AU2004284220B2 (en) 2010-10-14
AU2010236091A1 (en) 2010-11-25
SG147468A1 (en) 2008-11-28
WO2005040377A2 (en) 2005-05-06
US20130143264A1 (en) 2013-06-06
PL2292754T3 (en) 2013-06-28
ZA200604032B (en) 2008-05-28
CA2535836C (en) 2014-02-04
CN1863913B (en) 2011-01-12
EP2298898A1 (en) 2011-03-23
KR101229989B1 (en) 2013-02-06
HK1151824A1 (en) 2012-02-10
CN1863913A (en) 2006-11-15
EP2292754B1 (en) 2012-12-26
KR101230838B1 (en) 2013-02-06
US20070178469A1 (en) 2007-08-02
PL2298898T3 (en) 2013-05-31
EP1675952A2 (en) 2006-07-05
KR20060100372A (en) 2006-09-20
HK1149777A1 (en) 2011-10-14
RU2006117757A (en) 2007-11-27
KR20110112883A (en) 2011-10-13
HK1151825A1 (en) 2012-02-10
JP5396653B2 (en) 2014-01-22
IN2014DN02483A (en) 2015-05-15
PL2287302T3 (en) 2013-05-31
RU2375450C2 (en) 2009-12-10
AU2010236091B2 (en) 2011-07-07
AU2010236072A1 (en) 2010-11-18
KR101222025B1 (en) 2013-01-15
US10669562B2 (en) 2020-06-02
CA2535836A1 (en) 2005-05-06
EP2287302A1 (en) 2011-02-23
AU2010236093A1 (en) 2010-12-02

Similar Documents

Publication Publication Date Title
IN2014DN02484A (en)
EP1490489A4 (en) Methods and means for monitoring and modulating gene silencing
ES2375058T8 (en) AXMI-010, A GENE OF DELTA-ENDOTOXIN AND METHODS FOR USE.
HUP0402271A2 (en) Antimicrobial polypeptides from pseudoplectania nigrella
WO2001047944A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
HK1154623A1 (en) Isolated luciferases and the use thereof
WO1999057282A3 (en) Pesticidal toxins and nucleotide sequences which encode these toxins
WO2000029623A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
GB0020331D0 (en) Enzyme
ATE533779T1 (en) ISOLATED PHOTOPROTEIN MTCLYTIN AND USE THEREOF
WO2001048245A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2001040521A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2003089617A3 (en) Methods for increasing homologous recombination of a nucleic acid sequence
AU2003219134A1 (en) Identification of 3-ketosteroid 9-alfa-hydroxylase genes and microorganisms blocked in 3-ketosteroid 9-alfa-hydroxylase activity
WO2002072768A3 (en) ISOGENIC ss-CATENIN CELL LINES, AND METHODS OF MAKING AND USING SAME
MXPA02004782A (en) Sorghum dwarfing genes and methods of use.
AU2294102A (en) Mutant muscle-specific enhancers
AU2001276385A1 (en) Nucleotide sequences which code for the lysr3 gene
WO2001051670A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2000029622A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2005049818A3 (en) Genes encoding human deubiquitinating enzymes
WO2001038586A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
ATE346920T1 (en) NEW IMMUNO-REGULATORY PROTEIN LST-1
WO2001047942A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
NZ594522A (en) Manipulation of organic acid biosynthesis and secretion with cirate synthase