HUP0102517A2 - INFA2 kódoló régiójától 5' -irányban található genomi eredetű szekvenciák, alkalmazásuk INFA2-bevitelre alkalmas gyógyszerek előállítására, valamint eljárások INFA2 előállítására - Google Patents

INFA2 kódoló régiójától 5' -irányban található genomi eredetű szekvenciák, alkalmazásuk INFA2-bevitelre alkalmas gyógyszerek előállítására, valamint eljárások INFA2 előállítására

Info

Publication number
HUP0102517A2
HUP0102517A2 HU0102517A HUP0102517A HUP0102517A2 HU P0102517 A2 HUP0102517 A2 HU P0102517A2 HU 0102517 A HU0102517 A HU 0102517A HU P0102517 A HUP0102517 A HU P0102517A HU P0102517 A2 HUP0102517 A2 HU P0102517A2
Authority
HU
Hungary
Prior art keywords
cells
production
interferon
coding region
gene
Prior art date
Application number
HU0102517A
Other languages
English (en)
Inventor
Michael W. Heartlein
Richard F. Selden
Douglas A. Treco
Original Assignee
Transkaryotic Therapies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transkaryotic Therapies, Inc. filed Critical Transkaryotic Therapies, Inc.
Publication of HUP0102517A2 publication Critical patent/HUP0102517A2/hu
Publication of HUP0102517A3 publication Critical patent/HUP0102517A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Abstract

A találmány tárgya izolált nukleinsav, amely sztringens körülményekközött hibridizál, vagy legalább 80% azonosságot mutat egy, az INFA2-gént kódoló régiótól 5' irányban levő genomi régióval, és egy DNS-konstrukció, amely az izolált nukleinsavat homológ rekombinációkiváltására alkalmas célbajuttató szekvenciaként tartalmazza, valaminttartalmaz egy transzkripciós regulációs szekvenciát. A találmánytárgyát képezik továbbá a homológ rekombinációval létrejött sejtek,előnyösen emlőssejtek, a sejtek alkalmazása állatba Interferon-a-2bevitelére szolgáló gyógyszer előállítására, eljárások Interferon-a-2előállítására a sejtek előállítása és alkalmazása útján, valaminteljárások endogén Interferon-a-2 gén expressziójának megváltoztatásáraegy emlőssejtben in vitro. A találmány szerinti DNS-konstrukcióantivirális hatású, és hatásos a papilloma vírus, a hepatitis B és C,vaccinia, herpes simplex vírus, herpes zoster varicellosus vírus és arhinovírus okozta fertőzések kezelésében. Ó
HU0102517A 1998-05-07 1999-05-05 Genomic sequences upstream of the coding region of the ifn-alpha2 gene for protein production and delivery HUP0102517A3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8464898P 1998-05-07 1998-05-07
US8655598P 1998-05-21 1998-05-21
PCT/US1999/009925 WO1999057292A1 (en) 1998-05-07 1999-05-05 Genomic sequences upstream of the coding region of the ifn-alpha2 gene for protein production and delivery

Publications (2)

Publication Number Publication Date
HUP0102517A2 true HUP0102517A2 (hu) 2001-11-28
HUP0102517A3 HUP0102517A3 (en) 2004-06-28

Family

ID=26771248

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0102517A HUP0102517A3 (en) 1998-05-07 1999-05-05 Genomic sequences upstream of the coding region of the ifn-alpha2 gene for protein production and delivery

Country Status (14)

Country Link
EP (1) EP1075531A1 (hu)
JP (1) JP2002513580A (hu)
KR (1) KR20010052278A (hu)
CN (1) CN1309710A (hu)
AR (1) AR016264A1 (hu)
AU (1) AU3788899A (hu)
CA (1) CA2328459A1 (hu)
CZ (1) CZ299418B6 (hu)
HU (1) HUP0102517A3 (hu)
IL (1) IL139433A0 (hu)
NO (1) NO20005585L (hu)
PL (1) PL344517A1 (hu)
TR (1) TR200003271T2 (hu)
WO (1) WO1999057292A1 (hu)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001042442A2 (en) 1999-12-10 2001-06-14 Cytos Biotechnology Ag Activation of endogenous genes by genomic introduction of a replicon
FR2817559B1 (fr) * 2000-12-06 2003-12-12 Genodyssee Procede de determination d'un ou plusieurs polymorphisme(s) fontionnel(s) dans la sequence nucleique d'un gene "candidat" fonctionnel preselectionne et ses applications
CN103620034B (zh) * 2011-04-13 2016-12-14 加拿大国家研究委员会 具有来自IFNα2的SAR元件的表达系统

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery

Also Published As

Publication number Publication date
AU3788899A (en) 1999-11-23
JP2002513580A (ja) 2002-05-14
IL139433A0 (en) 2001-11-25
CN1309710A (zh) 2001-08-22
CZ299418B6 (cs) 2008-07-16
NO20005585D0 (no) 2000-11-06
NO20005585L (no) 2001-01-05
HUP0102517A3 (en) 2004-06-28
AR016264A1 (es) 2001-06-20
WO1999057292A1 (en) 1999-11-11
CA2328459A1 (en) 1999-11-11
PL344517A1 (en) 2001-11-05
CZ20003705A3 (cs) 2001-04-11
EP1075531A1 (en) 2001-02-14
WO1999057292A9 (en) 1999-12-16
TR200003271T2 (tr) 2001-03-21
KR20010052278A (ko) 2001-06-25

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