RU2300898C2 - Способ экстрагирования, очистки и ферментативной модификации альфа`субъединицы 7s-глобулина сои для использования в качестве гипохолестеринемического агента - Google Patents
Способ экстрагирования, очистки и ферментативной модификации альфа`субъединицы 7s-глобулина сои для использования в качестве гипохолестеринемического агента Download PDFInfo
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- RU2300898C2 RU2300898C2 RU2004123201/13A RU2004123201A RU2300898C2 RU 2300898 C2 RU2300898 C2 RU 2300898C2 RU 2004123201/13 A RU2004123201/13 A RU 2004123201/13A RU 2004123201 A RU2004123201 A RU 2004123201A RU 2300898 C2 RU2300898 C2 RU 2300898C2
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- Prior art keywords
- subunit
- conglycinin
- fraction
- purification
- treatment
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- 238000000746 purification Methods 0.000 title claims abstract description 14
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- 230000009144 enzymatic modification Effects 0.000 title claims abstract description 5
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 17
- 244000068988 Glycine max Species 0.000 claims abstract description 11
- 229920001184 polypeptide Polymers 0.000 claims abstract description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 11
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 11
- 108090000317 Chymotrypsin Proteins 0.000 claims abstract description 10
- 238000001042 affinity chromatography Methods 0.000 claims abstract description 7
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- 239000011701 zinc Substances 0.000 claims description 6
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- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 4
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- 229910052759 nickel Inorganic materials 0.000 claims description 2
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Images
Classifications
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- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/168—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Health & Medical Sciences (AREA)
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- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI2002A000147 | 2002-01-29 | ||
| IT2002MI000147A ITMI20020147A1 (it) | 2002-01-29 | 2002-01-29 | Estrazione purificazione e modificazione di polipeptidi da seme di soia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2004123201A RU2004123201A (ru) | 2005-10-27 |
| RU2300898C2 true RU2300898C2 (ru) | 2007-06-20 |
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|---|---|---|---|
| RU2004123201/13A RU2300898C2 (ru) | 2002-01-29 | 2003-01-27 | Способ экстрагирования, очистки и ферментативной модификации альфа`субъединицы 7s-глобулина сои для использования в качестве гипохолестеринемического агента |
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|---|---|
| US (3) | US7465467B2 (enExample) |
| EP (1) | EP1469741B1 (enExample) |
| JP (1) | JP4522706B2 (enExample) |
| KR (1) | KR100991641B1 (enExample) |
| CN (1) | CN100558243C (enExample) |
| AT (1) | ATE341943T1 (enExample) |
| AU (1) | AU2003208346B8 (enExample) |
| CA (1) | CA2474106C (enExample) |
| CY (1) | CY1107514T1 (enExample) |
| DE (1) | DE60308994T2 (enExample) |
| DK (1) | DK1469741T3 (enExample) |
| ES (1) | ES2274208T3 (enExample) |
| IL (1) | IL163225A (enExample) |
| IT (1) | ITMI20020147A1 (enExample) |
| NO (1) | NO327156B1 (enExample) |
| PL (1) | PL209342B1 (enExample) |
| PT (1) | PT1469741E (enExample) |
| RU (1) | RU2300898C2 (enExample) |
| SG (1) | SG152048A1 (enExample) |
| WO (1) | WO2003063608A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6171640B1 (en) * | 1997-04-04 | 2001-01-09 | Monsanto Company | High beta-conglycinin products and their use |
| EP1561384B1 (en) * | 2002-11-12 | 2007-10-10 | Fuji Oil Company, Ltd. | Fractionated soybean protein and process for producing the same |
| JP4687463B2 (ja) * | 2003-12-11 | 2011-05-25 | 不二製油株式会社 | 改良大豆７ｓたん白及びその製造法 |
| JP5382840B2 (ja) * | 2005-08-23 | 2014-01-08 | 特定非営利活動法人プライメイト・アゴラ | サルモデルで薬効が確認された骨粗鬆症の予防又は治療剤 |
| EP2384124B1 (en) * | 2008-12-31 | 2015-09-16 | Solae, Llc | Protein hydrolysate compositions |
| EP2264054A1 (en) * | 2009-06-17 | 2010-12-22 | Indena S.p.A. | Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin alfa ' subunit involved in Hep G2 cell cholesterol homeostasis |
| SI2264055T1 (sl) * | 2009-06-17 | 2014-05-30 | Indena S.P.A. | Kloniranje, izraĺ˝anje v kvasovki, äśiĺ äśenje in bioloĺ ka aktivnost ekstenzijske regije alfa'podenote sojinega 7s globulina udeleĺ˝ene pri homeostazi holesterola hepg2 celice |
| CN101904406B (zh) * | 2010-07-02 | 2012-07-25 | 山西大学 | 一种葵花籽多肽的制备方法和用途 |
| CN104163767B (zh) * | 2014-07-25 | 2017-01-18 | 湖北泓肽生物科技有限公司 | 一种氨基酸和多肽水溶性物质的纯化方法 |
| CN114874301A (zh) * | 2022-05-10 | 2022-08-09 | 吉林农业大学 | 不同亚基组成β-伴大豆球蛋白的制备方法及应用 |
Family Cites Families (13)
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| US4409248A (en) | 1981-08-10 | 1983-10-11 | A. E. Staley Manufacturing Company | Heat-gelling and foam-stabilizing enzymatically modified vegetable isolates |
| JPH06197788A (ja) | 1993-01-06 | 1994-07-19 | Fuji Oil Co Ltd | 蛋白質の製造法 |
| DE69700759T2 (de) * | 1996-03-28 | 2000-05-31 | Fuji Oil Co., Ltd. | Verfahren zur Herstellung eines Sojaproteinhydrolysats |
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