NO327156B1 - Fremgangsmate ved ekstraksjon, opprenskning og enzymatisk modifisering av soya 7S-globulin alfa-subenheten, slik modifisert subenhet samt anvendelse derav for fremstilling av hypokolesterolemiserende middel som farmasoytisk sammensetning eller matvaresupplement - Google Patents
Fremgangsmate ved ekstraksjon, opprenskning og enzymatisk modifisering av soya 7S-globulin alfa-subenheten, slik modifisert subenhet samt anvendelse derav for fremstilling av hypokolesterolemiserende middel som farmasoytisk sammensetning eller matvaresupplement Download PDFInfo
- Publication number
- NO327156B1 NO327156B1 NO20043186A NO20043186A NO327156B1 NO 327156 B1 NO327156 B1 NO 327156B1 NO 20043186 A NO20043186 A NO 20043186A NO 20043186 A NO20043186 A NO 20043186A NO 327156 B1 NO327156 B1 NO 327156B1
- Authority
- NO
- Norway
- Prior art keywords
- subunit
- conglycinin
- soy
- mac
- extraction
- Prior art date
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims description 31
- 238000000746 purification Methods 0.000 title claims description 9
- 230000009144 enzymatic modification Effects 0.000 title claims description 4
- 239000008194 pharmaceutical composition Substances 0.000 title claims description 4
- 238000002360 preparation method Methods 0.000 title claims description 4
- 230000008569 process Effects 0.000 title claims description 3
- 101000767750 Carya illinoinensis Vicilin Car i 2.0101 Proteins 0.000 title description 2
- 101000767759 Corylus avellana Vicilin Cor a 11.0101 Proteins 0.000 title description 2
- 101000622316 Juglans regia Vicilin Jug r 2.0101 Proteins 0.000 title description 2
- 101000767757 Pinus koraiensis Vicilin Pin k 2.0101 Proteins 0.000 title description 2
- 101000767758 Pistacia vera Vicilin Pis v 3.0101 Proteins 0.000 title description 2
- 235000015872 dietary supplement Nutrition 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000012634 fragment Substances 0.000 claims abstract description 17
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- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 238000001042 affinity chromatography Methods 0.000 claims abstract description 6
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims abstract description 5
- 229910052751 metal Inorganic materials 0.000 claims abstract description 5
- 239000002184 metal Substances 0.000 claims abstract description 5
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 10
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- 102000035195 Peptidases Human genes 0.000 claims description 5
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- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 229910052725 zinc Inorganic materials 0.000 claims description 5
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- 239000007864 aqueous solution Substances 0.000 abstract 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 12
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 12
- 235000010469 Glycine max Nutrition 0.000 description 11
- 239000000463 material Substances 0.000 description 11
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- 230000000260 hypercholesteremic effect Effects 0.000 description 7
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- 239000007858 starting material Substances 0.000 description 7
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- 235000012000 cholesterol Nutrition 0.000 description 5
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- 244000068988 Glycine max Species 0.000 description 3
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- 108010073771 Soybean Proteins Proteins 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
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Classifications
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/168—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
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- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
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- Wood Science & Technology (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Diabetes (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2002MI000147A ITMI20020147A1 (it) | 2002-01-29 | 2002-01-29 | Estrazione purificazione e modificazione di polipeptidi da seme di soia |
| PCT/EP2003/000798 WO2003063608A1 (en) | 2002-01-29 | 2003-01-27 | A process for the extraction, purification and enzymatic modification of soy 7s globulin alpha' subunit for use as hypocholesterolemizing agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO20043186L NO20043186L (no) | 2004-07-26 |
| NO327156B1 true NO327156B1 (no) | 2009-05-04 |
Family
ID=11449014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20043186A NO327156B1 (no) | 2002-01-29 | 2004-07-27 | Fremgangsmate ved ekstraksjon, opprenskning og enzymatisk modifisering av soya 7S-globulin alfa-subenheten, slik modifisert subenhet samt anvendelse derav for fremstilling av hypokolesterolemiserende middel som farmasoytisk sammensetning eller matvaresupplement |
Country Status (20)
| Country | Link |
|---|---|
| US (3) | US7465467B2 (enExample) |
| EP (1) | EP1469741B1 (enExample) |
| JP (1) | JP4522706B2 (enExample) |
| KR (1) | KR100991641B1 (enExample) |
| CN (1) | CN100558243C (enExample) |
| AT (1) | ATE341943T1 (enExample) |
| AU (1) | AU2003208346B8 (enExample) |
| CA (1) | CA2474106C (enExample) |
| CY (1) | CY1107514T1 (enExample) |
| DE (1) | DE60308994T2 (enExample) |
| DK (1) | DK1469741T3 (enExample) |
| ES (1) | ES2274208T3 (enExample) |
| IL (1) | IL163225A (enExample) |
| IT (1) | ITMI20020147A1 (enExample) |
| NO (1) | NO327156B1 (enExample) |
| PL (1) | PL209342B1 (enExample) |
| PT (1) | PT1469741E (enExample) |
| RU (1) | RU2300898C2 (enExample) |
| SG (1) | SG152048A1 (enExample) |
| WO (1) | WO2003063608A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6171640B1 (en) * | 1997-04-04 | 2001-01-09 | Monsanto Company | High beta-conglycinin products and their use |
| EP1561384B1 (en) * | 2002-11-12 | 2007-10-10 | Fuji Oil Company, Ltd. | Fractionated soybean protein and process for producing the same |
| JP4687463B2 (ja) * | 2003-12-11 | 2011-05-25 | 不二製油株式会社 | 改良大豆７ｓたん白及びその製造法 |
| JP5382840B2 (ja) * | 2005-08-23 | 2014-01-08 | 特定非営利活動法人プライメイト・アゴラ | サルモデルで薬効が確認された骨粗鬆症の予防又は治療剤 |
| EP2384124B1 (en) * | 2008-12-31 | 2015-09-16 | Solae, Llc | Protein hydrolysate compositions |
| EP2264054A1 (en) * | 2009-06-17 | 2010-12-22 | Indena S.p.A. | Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin alfa ' subunit involved in Hep G2 cell cholesterol homeostasis |
| SI2264055T1 (sl) * | 2009-06-17 | 2014-05-30 | Indena S.P.A. | Kloniranje, izraĺ˝anje v kvasovki, äśiĺ äśenje in bioloĺ ka aktivnost ekstenzijske regije alfa'podenote sojinega 7s globulina udeleĺ˝ene pri homeostazi holesterola hepg2 celice |
| CN101904406B (zh) * | 2010-07-02 | 2012-07-25 | 山西大学 | 一种葵花籽多肽的制备方法和用途 |
| CN104163767B (zh) * | 2014-07-25 | 2017-01-18 | 湖北泓肽生物科技有限公司 | 一种氨基酸和多肽水溶性物质的纯化方法 |
| CN114874301A (zh) * | 2022-05-10 | 2022-08-09 | 吉林农业大学 | 不同亚基组成β-伴大豆球蛋白的制备方法及应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4409248A (en) | 1981-08-10 | 1983-10-11 | A. E. Staley Manufacturing Company | Heat-gelling and foam-stabilizing enzymatically modified vegetable isolates |
| JPH06197788A (ja) | 1993-01-06 | 1994-07-19 | Fuji Oil Co Ltd | 蛋白質の製造法 |
| DE69700759T2 (de) * | 1996-03-28 | 2000-05-31 | Fuji Oil Co., Ltd. | Verfahren zur Herstellung eines Sojaproteinhydrolysats |
| ATE347604T1 (de) * | 1996-06-14 | 2006-12-15 | Du Pont | Suppressi0n spezifischer klassen von soja- sojasamenproteingenen |
| US6171640B1 (en) * | 1997-04-04 | 2001-01-09 | Monsanto Company | High beta-conglycinin products and their use |
| US6365802B2 (en) * | 1998-08-14 | 2002-04-02 | Calgene Llc | Methods for increasing stearate content in soybean oil |
| FR2784029B1 (fr) * | 1998-10-05 | 2001-01-05 | Pharmascience Lab | Methode de prevention et/ou de traitement cosmetique des vergetures de la peau et utilisation en dermatologie |
| MXPA01010486A (es) * | 1999-04-15 | 2002-03-27 | Calgene Llc | Secuencias de acido nucleico para proteinas implicadas en la sintesis de tocoferol. |
| CA2274414A1 (en) | 1999-06-11 | 2000-12-11 | Universite Laval | Dietary fish protein for use in restoring normal insulin function insulin-resistant individuals |
| AUPQ877300A0 (en) | 2000-07-13 | 2000-08-03 | Johnson & Johnson Pacific Pty Limited | Topical treatment of skin |
| KR101238752B1 (ko) * | 2002-04-18 | 2013-03-06 | 몬산토 테크놀로지 엘엘씨 | 심혈관 질환의 위험성을 감소시키기 위한 오일 바디 결합 단백질 조성물 및 이의 사용 방법 |
| EP1508573B1 (en) * | 2003-08-20 | 2009-10-28 | Seikagaku Corporation | Stabilizing agent for enzymes |
| EP1841333A1 (en) * | 2005-01-14 | 2007-10-10 | Solae, Llc | Soy protein for infant formula |
-
2002
- 2002-01-29 IT IT2002MI000147A patent/ITMI20020147A1/it unknown
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2003
- 2003-01-27 SG SG200506973-7A patent/SG152048A1/en unknown
- 2003-01-27 RU RU2004123201/13A patent/RU2300898C2/ru not_active IP Right Cessation
- 2003-01-27 US US10/502,582 patent/US7465467B2/en not_active Expired - Fee Related
- 2003-01-27 WO PCT/EP2003/000798 patent/WO2003063608A1/en not_active Ceased
- 2003-01-27 DK DK03706387T patent/DK1469741T3/da active
- 2003-01-27 AU AU2003208346A patent/AU2003208346B8/en not_active Ceased
- 2003-01-27 CA CA2474106A patent/CA2474106C/en not_active Expired - Fee Related
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- 2003-01-27 AT AT03706387T patent/ATE341943T1/de active
- 2003-01-27 JP JP2003563319A patent/JP4522706B2/ja not_active Expired - Fee Related
- 2003-01-27 ES ES03706387T patent/ES2274208T3/es not_active Expired - Lifetime
- 2003-01-27 KR KR1020047011441A patent/KR100991641B1/ko not_active Expired - Fee Related
- 2003-01-27 EP EP03706387A patent/EP1469741B1/en not_active Expired - Lifetime
- 2003-01-27 CN CNB038028050A patent/CN100558243C/zh not_active Expired - Fee Related
- 2003-01-27 DE DE60308994T patent/DE60308994T2/de not_active Expired - Lifetime
- 2003-01-27 PL PL370294A patent/PL209342B1/pl unknown
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- 2008-04-17 US US12/104,473 patent/US7901718B2/en not_active Expired - Fee Related
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2011
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