RU2009112104A - Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих - Google Patents
Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих Download PDFInfo
- Publication number
- RU2009112104A RU2009112104A RU2009112104/10A RU2009112104A RU2009112104A RU 2009112104 A RU2009112104 A RU 2009112104A RU 2009112104/10 A RU2009112104/10 A RU 2009112104/10A RU 2009112104 A RU2009112104 A RU 2009112104A RU 2009112104 A RU2009112104 A RU 2009112104A
- Authority
- RU
- Russia
- Prior art keywords
- trna
- amino acid
- host cell
- translation system
- specified
- Prior art date
Links
- 150000001413 amino acids Chemical class 0.000 title claims abstract 35
- 102000004169 proteins and genes Human genes 0.000 title claims 9
- 108090000623 proteins and genes Proteins 0.000 title claims 9
- 241000124008 Mammalia Species 0.000 title 1
- 230000002068 genetic effect Effects 0.000 title 1
- 230000010354 integration Effects 0.000 title 1
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims abstract 13
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims abstract 13
- 108091033319 polynucleotide Proteins 0.000 claims abstract 10
- 102000040430 polynucleotide Human genes 0.000 claims abstract 10
- 239000002157 polynucleotide Substances 0.000 claims abstract 10
- DOCYTUNUHIGJTI-QMMMGPOBSA-N (2r)-2-[(2-nitrophenyl)methylamino]-3-sulfanylpropanoic acid Chemical compound OC(=O)[C@H](CS)NCC1=CC=CC=C1[N+]([O-])=O DOCYTUNUHIGJTI-QMMMGPOBSA-N 0.000 claims abstract 6
- ZXSBHXZKWRIEIA-JTQLQIEISA-N (2s)-3-(4-acetylphenyl)-2-azaniumylpropanoate Chemical compound CC(=O)C1=CC=C(C[C@H](N)C(O)=O)C=C1 ZXSBHXZKWRIEIA-JTQLQIEISA-N 0.000 claims abstract 6
- 241000288906 Primates Species 0.000 claims abstract 5
- 241000283984 Rodentia Species 0.000 claims abstract 4
- VKBLQCDGTHFOLS-NSHDSACASA-N (2s)-2-(4-benzoylanilino)propanoic acid Chemical compound C1=CC(N[C@@H](C)C(O)=O)=CC=C1C(=O)C1=CC=CC=C1 VKBLQCDGTHFOLS-NSHDSACASA-N 0.000 claims abstract 3
- NEMHIKRLROONTL-QMMMGPOBSA-N (2s)-2-azaniumyl-3-(4-azidophenyl)propanoate Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1 NEMHIKRLROONTL-QMMMGPOBSA-N 0.000 claims abstract 3
- AKVBCGQVQXPRLD-UHFFFAOYSA-N 2-aminooctanoic acid Chemical compound CCCCCCC(N)C(O)=O AKVBCGQVQXPRLD-UHFFFAOYSA-N 0.000 claims abstract 3
- 241000588724 Escherichia coli Species 0.000 claims abstract 3
- 241001425800 Pipa Species 0.000 claims abstract 3
- 101100191375 Xenopus laevis prkra-b gene Proteins 0.000 claims abstract 3
- 241000186394 Eubacterium Species 0.000 claims abstract 2
- GEYBMYRBIABFTA-VIFPVBQESA-N O-methyl-L-tyrosine Chemical compound COC1=CC=C(C[C@H](N)C(O)=O)C=C1 GEYBMYRBIABFTA-VIFPVBQESA-N 0.000 claims abstract 2
- 230000000689 aminoacylating effect Effects 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims 17
- 108020004705 Codon Proteins 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 238000002741 site-directed mutagenesis Methods 0.000 claims 2
- QHGDJQUCSGUYMF-QMMMGPOBSA-N (2s)-3-hydroxy-2-[(2-nitrophenyl)methylamino]propanoic acid Chemical compound OC[C@@H](C(O)=O)NCC1=CC=CC=C1[N+]([O-])=O QHGDJQUCSGUYMF-QMMMGPOBSA-N 0.000 claims 1
- PZNQZSRPDOEBMS-QMMMGPOBSA-N 4-iodo-L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(I)C=C1 PZNQZSRPDOEBMS-QMMMGPOBSA-N 0.000 claims 1
- 241000282693 Cercopithecidae Species 0.000 claims 1
- 241000282575 Gorilla Species 0.000 claims 1
- 241000282620 Hylobates sp. Species 0.000 claims 1
- 241000282553 Macaca Species 0.000 claims 1
- 241000282576 Pan paniscus Species 0.000 claims 1
- 241000282577 Pan troglodytes Species 0.000 claims 1
- 241000282405 Pongo abelii Species 0.000 claims 1
- 241000288961 Saguinus imperator Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- RCRODHONKLSMIF-UHFFFAOYSA-N isosuberenol Natural products O1C(=O)C=CC2=C1C=C(OC)C(CC(O)C(C)=C)=C2 RCRODHONKLSMIF-UHFFFAOYSA-N 0.000 claims 1
- 230000004952 protein activity Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85300806P | 2006-10-18 | 2006-10-18 | |
| US60/853,008 | 2006-10-18 | ||
| US92345807P | 2007-04-12 | 2007-04-12 | |
| US60/923,458 | 2007-04-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2009112104A true RU2009112104A (ru) | 2010-11-27 |
Family
ID=39512252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009112104/10A RU2009112104A (ru) | 2006-10-18 | 2007-10-17 | Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20080166766A1 (https=) |
| EP (1) | EP2069481A4 (https=) |
| JP (1) | JP2010506591A (https=) |
| KR (1) | KR20090094812A (https=) |
| CN (1) | CN101535338A (https=) |
| AU (1) | AU2007333010A1 (https=) |
| BR (1) | BRPI0718389A2 (https=) |
| CA (1) | CA2665678A1 (https=) |
| IL (1) | IL197917A0 (https=) |
| MX (1) | MX2009004043A (https=) |
| RU (1) | RU2009112104A (https=) |
| WO (1) | WO2008073184A2 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7083970B2 (en) * | 2001-04-19 | 2006-08-01 | The Scripps Research Institute | Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs |
| MXPA05003978A (es) * | 2002-10-16 | 2005-06-22 | Scripps Research Inst | Sintesis de glicoproteinas. |
| WO2004094593A2 (en) | 2003-04-17 | 2004-11-04 | The Scripps Research Institute | Expanding the eukaryotic genetic code |
| WO2005019415A2 (en) | 2003-07-07 | 2005-03-03 | The Scripps Research Institute | Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| WO2010114615A2 (en) * | 2009-04-03 | 2010-10-07 | The Scripps Research Institute | A facile system for encoding unnatural amino acids in mammalian cells |
| CN103328648B (zh) * | 2010-12-03 | 2015-11-25 | 国立大学法人东京大学 | 具有稳定的二级结构的肽和肽库以及它们的制造方法 |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| SG10201702387YA (en) * | 2012-09-24 | 2017-04-27 | Medimmune Ltd | Cell lines |
| BR112015022641B1 (pt) * | 2013-03-15 | 2023-02-28 | Medimmune Limited | Construção de dna e processo de preparação de uma proteína-alvo |
| CN109295025B (zh) * | 2014-09-03 | 2021-04-02 | 中国科学院生物物理研究所 | 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族 |
| CN105400816A (zh) * | 2015-11-24 | 2016-03-16 | 深圳市第二人民医院 | 用于膀胱癌检测的逻辑与门基因线路的构建方法 |
| GB201600512D0 (en) | 2016-01-12 | 2016-02-24 | Univ York | Recombinant protein production |
| CN112725282A (zh) * | 2016-01-27 | 2021-04-30 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| CN110520522A (zh) | 2017-02-17 | 2019-11-29 | 鲁比厄斯治疗法股份有限公司 | 功能化红系细胞 |
| EP3630977B1 (en) * | 2017-06-02 | 2024-02-21 | Ambrx, Inc. | Methods and compositions for promoting non-natural amino acid-containing protein production |
| JP2020522263A (ja) | 2017-06-06 | 2020-07-30 | キンドレッド バイオサイエンシズ インコーポレイテッド | 獣医学的使用のためのエリスロポエチンおよびアナログ |
| JP2021520828A (ja) | 2018-04-19 | 2021-08-26 | キンドレッド バイオサイエンシズ インコーポレイテッド | 医療用の変異体アスパラギナーゼポリペプチド |
| CN112673109A (zh) * | 2018-09-07 | 2021-04-16 | 免疫医疗有限责任公司 | 细胞选择方法 |
| US12565646B2 (en) | 2019-06-21 | 2026-03-03 | Trustees Of Boston College | Enhanced platforms for unnatural amino acid incorporation in mammalian cells |
| CN114729334A (zh) * | 2019-08-08 | 2022-07-08 | 布里克生物公司 | 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系 |
| KR20220080101A (ko) * | 2019-10-15 | 2022-06-14 | 트러스티스 오브 보스톤 칼리지 | 향상된 비천연 아미노산 혼입을 위한 키메라 열안정성 아미노아실-tRNA 합성효소 |
| US20230069218A1 (en) * | 2019-12-12 | 2023-03-02 | Chugai Seiyaku Kabushiki Kaisha | Method for producing peptide containing non-natural amino acid |
| CN113388654A (zh) * | 2021-06-04 | 2021-09-14 | 清华大学 | 一种基于线形模板无细胞体系非天然氨基酸嵌入方法 |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN119662697B (zh) * | 2024-12-20 | 2026-03-27 | 天津大学 | 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7083970B2 (en) | 2001-04-19 | 2006-08-01 | The Scripps Research Institute | Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs |
| MXPA05003978A (es) | 2002-10-16 | 2005-06-22 | Scripps Research Inst | Sintesis de glicoproteinas. |
| ES2324608T3 (es) | 2002-10-16 | 2009-08-11 | The Scripps Research Institute | Incorporacion especifica de sitio de cetoaminoacidos en proteinas. |
| WO2004039989A1 (ja) * | 2002-10-31 | 2004-05-13 | Riken | 非天然型アミノ酸組み込みタンパク質の発現方法 |
| WO2004058946A2 (en) | 2002-12-22 | 2004-07-15 | The Scripps Research Institute | Protein arrays |
| JP2006517802A (ja) * | 2003-02-14 | 2006-08-03 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 外来性分子の一過性の発現もしくは安定な発現のための発現カセットおよび発現ベクター |
| WO2004094593A2 (en) | 2003-04-17 | 2004-11-04 | The Scripps Research Institute | Expanding the eukaryotic genetic code |
| JP5275566B2 (ja) | 2003-06-18 | 2013-08-28 | ザ スクリプス リサーチ インスティチュート | 反応性非天然アミノ酸遺伝コード付加 |
| WO2005019415A2 (en) | 2003-07-07 | 2005-03-03 | The Scripps Research Institute | Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| US20060160175A1 (en) | 2003-07-07 | 2006-07-20 | The Scripps Research Institute | Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| JP5657850B2 (ja) | 2003-10-14 | 2015-01-21 | ザ スクリプス リサーチ インスティテュート | 酸化還元機能性アミノ酸のタンパク質への部位特異的組み入れ |
| AU2004321117B2 (en) | 2003-12-18 | 2010-09-02 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
| EP2327724A3 (en) * | 2004-02-02 | 2011-07-27 | Ambrx, Inc. | Modified human growth hormone polypeptides and their uses |
| AU2005248392A1 (en) | 2004-05-25 | 2005-12-08 | Irm, Llc | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
| CA2580840A1 (en) | 2004-09-21 | 2006-03-30 | The Scripps Research Institute | In vivo incorporation of alkynyl amino acids into proteins in eubacteria |
| US20090181858A1 (en) * | 2004-09-21 | 2009-07-16 | The Scripps Research Institute | Adding photoregulated amino acids to the genetic code |
| US20080171317A1 (en) | 2004-09-22 | 2008-07-17 | The Scripps Research Institute | Site-Specific Labeling of Proteins for Nmr Studies |
| JP2008516637A (ja) | 2004-10-20 | 2008-05-22 | ザ スクリップス リサーチ インスティテュート | 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み |
| EP1807515A4 (en) | 2004-10-27 | 2008-06-18 | Scripps Research Inst | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| US8367588B2 (en) | 2005-10-12 | 2013-02-05 | The Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| US7960141B2 (en) | 2006-03-09 | 2011-06-14 | The Scripps Research Institute | Systems for the expression of orthogonal translation components in eubacterial host cells |
| KR20080113226A (ko) | 2006-03-16 | 2008-12-29 | 더 스크립스 리서치 인스티튜트 | 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현 |
-
2007
- 2007-10-17 JP JP2009533372A patent/JP2010506591A/ja active Pending
- 2007-10-17 WO PCT/US2007/022232 patent/WO2008073184A2/en not_active Ceased
- 2007-10-17 EP EP07870795A patent/EP2069481A4/en not_active Withdrawn
- 2007-10-17 CA CA002665678A patent/CA2665678A1/en not_active Abandoned
- 2007-10-17 CN CNA2007800390157A patent/CN101535338A/zh active Pending
- 2007-10-17 US US11/975,351 patent/US20080166766A1/en not_active Abandoned
- 2007-10-17 AU AU2007333010A patent/AU2007333010A1/en not_active Abandoned
- 2007-10-17 KR KR1020097009346A patent/KR20090094812A/ko not_active Ceased
- 2007-10-17 MX MX2009004043A patent/MX2009004043A/es not_active Application Discontinuation
- 2007-10-17 US US12/311,545 patent/US9624485B2/en not_active Expired - Fee Related
- 2007-10-17 BR BRPI0718389-5A patent/BRPI0718389A2/pt not_active IP Right Cessation
- 2007-10-17 RU RU2009112104/10A patent/RU2009112104A/ru not_active Application Discontinuation
-
2009
- 2009-04-05 IL IL197917A patent/IL197917A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2069481A4 (en) | 2010-01-06 |
| KR20090094812A (ko) | 2009-09-08 |
| IL197917A0 (en) | 2011-08-01 |
| WO2008073184A2 (en) | 2008-06-19 |
| CA2665678A1 (en) | 2008-06-19 |
| EP2069481A2 (en) | 2009-06-17 |
| US20100021963A1 (en) | 2010-01-28 |
| BRPI0718389A2 (pt) | 2012-04-10 |
| AU2007333010A1 (en) | 2008-06-19 |
| MX2009004043A (es) | 2009-04-27 |
| JP2010506591A (ja) | 2010-03-04 |
| US20080166766A1 (en) | 2008-07-10 |
| US9624485B2 (en) | 2017-04-18 |
| CN101535338A (zh) | 2009-09-16 |
| WO2008073184A3 (en) | 2008-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2009112104A (ru) | Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих | |
| JP2010506591A5 (https=) | ||
| JP6294368B2 (ja) | 直交ｔＲＮＡ−アミノアシルｔＲＮＡシンテターゼ対を生産するための方法及び組成物 | |
| RU2008145487A (ru) | Генетически кодируемые флюоресцентные кумариновые аминокислоты | |
| JP2008516637A5 (https=) | ||
| EP3487998B1 (en) | Compositions and methods for identifying rna binding polypeptide targets | |
| AU2002303431A1 (en) | Methods and composition for the production of orthoganal tRNA-aminoacyltRNA synthetase pairs | |
| JP2008513040A5 (https=) | ||
| Guo et al. | Genetic code expansion through quadruplet codon decoding | |
| RU2005114367A (ru) | Кольцевая молекула днк, характеризующаяся зависимой от условий точкой начала репликации, способ их получения и их применение в генной терапии | |
| AU2014369177A1 (en) | Novel eukaryotic cells and methods for recombinantly expressing a product of interest | |
| CN109022285B (zh) | 一种提高集胞藻pcc6803铵盐耐受能力的方法与应用 | |
| BR0309401A (pt) | polinucleotìdeo isolado, vetor de expressão, célula cultivada, método para produzir polipeptìdeos, e um anticorpo para um polipeptìdeo, e, anticorpo | |
| JP7340372B2 (ja) | 光活性化アデニル酸シクラーゼ | |
| CN110257415B (zh) | 一种用于金属离子检测的核酸蛋白复合型微生物全细胞传感器构建及阈值调控技术 | |
| Gurskaya et al. | The coding region of far-red fluorescent protein Katushka contains a strong donor splice site | |
| CN120758499A (zh) | 用于序列特异性通读终止密码子的tRNA解码器 | |
| CN117286161B (zh) | 麦草畏厌氧降解中间产物3，6-二氯水杨酸脱羧酶CsaDC及其应用 | |
| CN102181465B (zh) | 一种荧光筛选克隆载体及其制备与应用 | |
| AU2007251897B2 (en) | Methods and compositions for the production of orthoganal tRNA-aminoacyltRNA synthetase pairs | |
| RU2790662C1 (ru) | АМИНОАЦИЛ-тРНК-СИНТЕТАЗА, ЭФФЕКТИВНОЕ ВВЕДЕНИЕ ПРОИЗВОДНЫХ ЛИЗИНА | |
| Kröger | THE SWEETNESS OF DIATOM MOLECULAR ENGINEERING. | |
| Ware et al. | Identifying the gene responsible for non-photochemical | |
| Mogi et al. | A high-yield protein expression platform in the unicellular red alga Cyanidioschyzon merolae | |
| WO2025130719A1 (zh) | 诱导蛋白自行降解的重组盐单胞菌及其构建方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20120606 |