CA2665678A1 - Genetic incorporation of unnatural amino acids into proteins in mammalian cells - Google Patents
Genetic incorporation of unnatural amino acids into proteins in mammalian cells Download PDFInfo
- Publication number
- CA2665678A1 CA2665678A1 CA002665678A CA2665678A CA2665678A1 CA 2665678 A1 CA2665678 A1 CA 2665678A1 CA 002665678 A CA002665678 A CA 002665678A CA 2665678 A CA2665678 A CA 2665678A CA 2665678 A1 CA2665678 A1 CA 2665678A1
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- Prior art keywords
- trna
- amino acid
- unnatural amino
- host cell
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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| PCT/US2007/022232 WO2008073184A2 (en) | 2006-10-18 | 2007-10-17 | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
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| CN114134141A (zh) * | 2021-11-03 | 2022-03-04 | 浙江大学 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
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| MXPA05003978A (es) * | 2002-10-16 | 2005-06-22 | Scripps Research Inst | Sintesis de glicoproteinas. |
| JP5642916B2 (ja) | 2003-04-17 | 2014-12-17 | ザ スクリプス リサーチ インスティテュート | 真核遺伝コードの拡張 |
| EP1649004A4 (en) * | 2003-07-07 | 2008-04-09 | Scripps Research Inst | COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF |
| WO2010114615A2 (en) * | 2009-04-03 | 2010-10-07 | The Scripps Research Institute | A facile system for encoding unnatural amino acids in mammalian cells |
| EP2647721B1 (en) * | 2010-12-03 | 2019-02-27 | The University of Tokyo | Peptide with safer secondary structure, peptide library, and production methods for same |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| EP2898085B1 (en) * | 2012-09-24 | 2019-01-23 | MedImmune Limited | Cell lines |
| AU2014229999B2 (en) * | 2013-03-15 | 2019-09-12 | Medimmune Limited | Novel nucleic acid molecules |
| CN105420203B (zh) * | 2014-09-03 | 2018-11-16 | 中国科学院生物物理研究所 | 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族 |
| CN105400816A (zh) * | 2015-11-24 | 2016-03-16 | 深圳市第二人民医院 | 用于膀胱癌检测的逻辑与门基因线路的构建方法 |
| GB201600512D0 (en) | 2016-01-12 | 2016-02-24 | Univ York | Recombinant protein production |
| CN107012121B (zh) * | 2016-01-27 | 2021-01-26 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| CN110520522A (zh) | 2017-02-17 | 2019-11-29 | 鲁比厄斯治疗法股份有限公司 | 功能化红系细胞 |
| PL3630977T3 (pl) * | 2017-06-02 | 2024-06-24 | Ambrx, Inc. | Sposoby i kompozycje promujące produkcję białek zawierających nienaturalne aminokwasy |
| AU2018281334A1 (en) | 2017-06-06 | 2019-12-19 | Kindred Biosciences, Inc. | Erythropoietin and analogs for veterinary use |
| JP2021520828A (ja) | 2018-04-19 | 2021-08-26 | キンドレッド バイオサイエンシズ インコーポレイテッド | 医療用の変異体アスパラギナーゼポリペプチド |
| CN112673109A (zh) * | 2018-09-07 | 2021-04-16 | 免疫医疗有限责任公司 | 细胞选择方法 |
| AU2020295571B2 (en) | 2019-06-21 | 2026-03-26 | Trustees Of Boston College | Enhanced platforms for unnatural amino acid incorporation in mammalian cells |
| US20220325269A1 (en) * | 2019-08-08 | 2022-10-13 | Brickbio, Inc. | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
| US20230279378A1 (en) * | 2019-10-15 | 2023-09-07 | Trustees Of Boston College | Chimeric thermostable aminoacyl-trna synthetase for enhanced unnatural amino acid incorporation |
| JP7267458B2 (ja) * | 2019-12-12 | 2023-05-01 | 中外製薬株式会社 | 非天然アミノ酸を含むペプチドの製造方法 |
| CN113388654A (zh) * | 2021-06-04 | 2021-09-14 | 清华大学 | 一种基于线形模板无细胞体系非天然氨基酸嵌入方法 |
| CN119662697B (zh) * | 2024-12-20 | 2026-03-27 | 天津大学 | 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用 |
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| JP4444113B2 (ja) | 2002-10-16 | 2010-03-31 | ザ スクリップス リサーチ インスティテュート | ケトアミノ酸の部位特異的蛋白質組込み方法 |
| US7566555B2 (en) * | 2002-10-31 | 2009-07-28 | Riken | Method of expressing proteins comprising non-naturally-occurring amino acids |
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| EP2410331B1 (en) * | 2003-06-18 | 2015-09-23 | The Scripps Research Institute | Aminoacyl-tRNA synthetase for aminoacylation tRNA with unnatural amino acids |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| US20060160175A1 (en) | 2003-07-07 | 2006-07-20 | The Scripps Research Institute | Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| EP1649004A4 (en) | 2003-07-07 | 2008-04-09 | Scripps Research Inst | COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF |
| PT1687401E (pt) | 2003-10-14 | 2013-02-26 | Scripps Research Inst | Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas |
| US7741071B2 (en) | 2003-12-18 | 2010-06-22 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
| BRPI0507169A (pt) * | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos |
| JP2008500050A (ja) | 2004-05-25 | 2008-01-10 | ザ スクリップス リサーチ インスティテュート | 結晶構造決定のための重原子含有非天然アミノ酸の部位特異的蛋白質組込み |
| AU2005286722B2 (en) | 2004-09-21 | 2010-12-02 | The Scripps Research Institute | In vivo incorporation of alkynyl amino acids into proteins in eubacteria |
| US20090181858A1 (en) * | 2004-09-21 | 2009-07-16 | The Scripps Research Institute | Adding photoregulated amino acids to the genetic code |
| US20080171317A1 (en) | 2004-09-22 | 2008-07-17 | The Scripps Research Institute | Site-Specific Labeling of Proteins for Nmr Studies |
| JP2008516637A (ja) | 2004-10-20 | 2008-05-22 | ザ スクリップス リサーチ インスティテュート | 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み |
| CN101048506B (zh) | 2004-10-27 | 2013-06-26 | 斯克利普斯研究院 | 体内掺入非天然氨基酸的正交翻译组分 |
| BRPI0617191A2 (pt) | 2005-10-12 | 2011-07-19 | Scripps Research Inst | modificação pós-traducional de polipeptìdeos expressos em fagos |
| KR20080106430A (ko) | 2006-03-09 | 2008-12-05 | 더 스크립스 리서치 인스티튜트 | 진정 세균 숙주 세포 내에서의 직교형 번역 성분의 발현을 위한 시스템 |
| WO2007109035A2 (en) | 2006-03-16 | 2007-09-27 | The Scripps Research Institute | Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine |
-
2007
- 2007-10-17 WO PCT/US2007/022232 patent/WO2008073184A2/en not_active Ceased
- 2007-10-17 US US11/975,351 patent/US20080166766A1/en not_active Abandoned
- 2007-10-17 KR KR1020097009346A patent/KR20090094812A/ko not_active Ceased
- 2007-10-17 BR BRPI0718389-5A patent/BRPI0718389A2/pt not_active IP Right Cessation
- 2007-10-17 JP JP2009533372A patent/JP2010506591A/ja active Pending
- 2007-10-17 CN CNA2007800390157A patent/CN101535338A/zh active Pending
- 2007-10-17 RU RU2009112104/10A patent/RU2009112104A/ru not_active Application Discontinuation
- 2007-10-17 EP EP07870795A patent/EP2069481A4/en not_active Withdrawn
- 2007-10-17 US US12/311,545 patent/US9624485B2/en not_active Expired - Fee Related
- 2007-10-17 AU AU2007333010A patent/AU2007333010A1/en not_active Abandoned
- 2007-10-17 MX MX2009004043A patent/MX2009004043A/es not_active Application Discontinuation
- 2007-10-17 CA CA002665678A patent/CA2665678A1/en not_active Abandoned
-
2009
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134141A (zh) * | 2021-11-03 | 2022-03-04 | 浙江大学 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010506591A (ja) | 2010-03-04 |
| US20100021963A1 (en) | 2010-01-28 |
| EP2069481A2 (en) | 2009-06-17 |
| CN101535338A (zh) | 2009-09-16 |
| BRPI0718389A2 (pt) | 2012-04-10 |
| RU2009112104A (ru) | 2010-11-27 |
| MX2009004043A (es) | 2009-04-27 |
| AU2007333010A1 (en) | 2008-06-19 |
| KR20090094812A (ko) | 2009-09-08 |
| IL197917A0 (en) | 2011-08-01 |
| WO2008073184A3 (en) | 2008-11-27 |
| EP2069481A4 (en) | 2010-01-06 |
| US20080166766A1 (en) | 2008-07-10 |
| US9624485B2 (en) | 2017-04-18 |
| WO2008073184A2 (en) | 2008-06-19 |
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