MX2009004043A - Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. - Google Patents

Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Info

Publication number
MX2009004043A
MX2009004043A MX2009004043A MX2009004043A MX2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A
Authority
MX
Mexico
Prior art keywords
unnatural amino
proteins
amino acids
host cells
mammalian cells
Prior art date
Application number
MX2009004043A
Other languages
English (en)
Spanish (es)
Inventor
Peter G Schultz
Wenshe Liu
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2009004043A publication Critical patent/MX2009004043A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
MX2009004043A 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. MX2009004043A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Publications (1)

Publication Number Publication Date
MX2009004043A true MX2009004043A (es) 2009-04-27

Family

ID=39512252

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009004043A MX2009004043A (es) 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Country Status (12)

Country Link
US (2) US20080166766A1 (https=)
EP (1) EP2069481A4 (https=)
JP (1) JP2010506591A (https=)
KR (1) KR20090094812A (https=)
CN (1) CN101535338A (https=)
AU (1) AU2007333010A1 (https=)
BR (1) BRPI0718389A2 (https=)
CA (1) CA2665678A1 (https=)
IL (1) IL197917A0 (https=)
MX (1) MX2009004043A (https=)
RU (1) RU2009112104A (https=)
WO (1) WO2008073184A2 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP1649004A4 (en) * 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
EP2647721B1 (en) * 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
EP2898085B1 (en) * 2012-09-24 2019-01-23 MedImmune Limited Cell lines
AU2014229999B2 (en) * 2013-03-15 2019-09-12 Medimmune Limited Novel nucleic acid molecules
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN107012121B (zh) * 2016-01-27 2021-01-26 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110520522A (zh) 2017-02-17 2019-11-29 鲁比厄斯治疗法股份有限公司 功能化红系细胞
PL3630977T3 (pl) * 2017-06-02 2024-06-24 Ambrx, Inc. Sposoby i kompozycje promujące produkcję białek zawierających nienaturalne aminokwasy
AU2018281334A1 (en) 2017-06-06 2019-12-19 Kindred Biosciences, Inc. Erythropoietin and analogs for veterinary use
JP2021520828A (ja) 2018-04-19 2021-08-26 キンドレッド バイオサイエンシズ インコーポレイテッド 医療用の変異体アスパラギナーゼポリペプチド
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
AU2020295571B2 (en) 2019-06-21 2026-03-26 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
US20230279378A1 (en) * 2019-10-15 2023-09-07 Trustees Of Boston College Chimeric thermostable aminoacyl-trna synthetase for enhanced unnatural amino acid incorporation
JP7267458B2 (ja) * 2019-12-12 2023-05-01 中外製薬株式会社 非天然アミノ酸を含むペプチドの製造方法
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697B (zh) * 2024-12-20 2026-03-27 天津大学 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP4444113B2 (ja) 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
US7566555B2 (en) * 2002-10-31 2009-07-28 Riken Method of expressing proteins comprising non-naturally-occurring amino acids
CA2508939A1 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
DK1594955T3 (da) * 2003-02-14 2012-06-25 Biogen Idec Inc Ekspressionskassette og vektor til transient eller stabil ekspression af eksogene molekyler
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2410331B1 (en) * 2003-06-18 2015-09-23 The Scripps Research Institute Aminoacyl-tRNA synthetase for aminoacylation tRNA with unnatural amino acids
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
EP1649004A4 (en) 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
PT1687401E (pt) 2003-10-14 2013-02-26 Scripps Research Inst Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas
US7741071B2 (en) 2003-12-18 2010-06-22 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
BRPI0507169A (pt) * 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos
JP2008500050A (ja) 2004-05-25 2008-01-10 ザ スクリップス リサーチ インスティテュート 結晶構造決定のための重原子含有非天然アミノ酸の部位特異的蛋白質組込み
AU2005286722B2 (en) 2004-09-21 2010-12-02 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
US20080171317A1 (en) 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
JP2008516637A (ja) 2004-10-20 2008-05-22 ザ スクリップス リサーチ インスティテュート 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み
CN101048506B (zh) 2004-10-27 2013-06-26 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
BRPI0617191A2 (pt) 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
KR20080106430A (ko) 2006-03-09 2008-12-05 더 스크립스 리서치 인스티튜트 진정 세균 숙주 세포 내에서의 직교형 번역 성분의 발현을 위한 시스템
WO2007109035A2 (en) 2006-03-16 2007-09-27 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Also Published As

Publication number Publication date
JP2010506591A (ja) 2010-03-04
US20100021963A1 (en) 2010-01-28
EP2069481A2 (en) 2009-06-17
CN101535338A (zh) 2009-09-16
BRPI0718389A2 (pt) 2012-04-10
RU2009112104A (ru) 2010-11-27
AU2007333010A1 (en) 2008-06-19
KR20090094812A (ko) 2009-09-08
IL197917A0 (en) 2011-08-01
WO2008073184A3 (en) 2008-11-27
EP2069481A4 (en) 2010-01-06
CA2665678A1 (en) 2008-06-19
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
WO2008073184A2 (en) 2008-06-19

Similar Documents

Publication Publication Date Title
MX2009004043A (es) Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.
WO2006110182A3 (en) Orthogonal translation components for the in vivo incorporation of unnatural amino acids
MX2008011669A (es) Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.
MX2009002987A (es) Expresion geneticamente programada de proteinas selectivamente sulfatadas en eubacterias.
IL196893A (en) Cell or cell line of vertebrate-containing amino acids trna synthase
IL171128A (en) Eukaryotic cell that incorporates unnatural amino acid into a protein and a method for producing the protein through it
MX2007003142A (es) Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias.
WO2005003294A3 (en) Unnatural reactive amino acid genetic code additions
MX2009002526A (es) Transcripcion de tarn supresor en celulas de vertebrados.
WO2007047301A3 (en) Selective posttranslational modification of phage-displayed polypeptides
ES2353814T3 (es) Proteinas n-glicosiladas recombinantes de celulas procariotas.
PT1991680E (pt) Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas
MX2007002380A (es) Produccion de polipeptidos.
BRPI0519568B8 (pt) aminoacil-trna sintetase (rs), composições que compreendem a dita aminoacil-trna sintetase, célula de levedura, célula fúngica, célula não eucariótica, vetor que codifica uma rs, polinucleotídeo e ainda método para produzir um polipeptídeo em uma célula com um aminoácido selecionado em uma posição especificada
WO2002086075A3 (en) Methods and composition for the production of orthoganal trna-aminoacyltrna synthetase pairs
WO2005038002A3 (en) Site-specific incorporation of redox active amino acids into proteins
WO2011075686A3 (en) Methods & compositions comprising heat shock proteins
WO2008030613A3 (en) Hybrid suppressor trna for vertebrate cells
JP2008516637A5 (https=)
EP4241854A3 (en) Lysosomal storage disease enzyme
JP2009538136A5 (https=)
WO2009066320A3 (en) Gcsf fusion protein systems suitable for high expression of peptides
WO2014194129A3 (en) Cell-free synthetic incorporation of non-natural amino acids into proteins
NO20072998L (no) Stabilisering av glukokortikoidestere med syrer
WO2010114615A3 (en) A facile system for encoding unnatural amino acids in mammalian cells

Legal Events

Date Code Title Description
FA Abandonment or withdrawal