JP2010506591A - 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み - Google Patents

哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み Download PDF

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JP2010506591A
JP2010506591A JP2009533372A JP2009533372A JP2010506591A JP 2010506591 A JP2010506591 A JP 2010506591A JP 2009533372 A JP2009533372 A JP 2009533372A JP 2009533372 A JP2009533372 A JP 2009533372A JP 2010506591 A JP2010506591 A JP 2010506591A
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trna
amino acid
unnatural amino
cells
amino acids
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JP2010506591A5 (https=
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ウェンシー・リウ
ピーター・ジー.・シュルツ
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Scripps Research Institute
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
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    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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JP2009533372A 2006-10-18 2007-10-17 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み Pending JP2010506591A (ja)

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US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

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JP2010506591A5 JP2010506591A5 (https=) 2010-12-09

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US (2) US20080166766A1 (https=)
EP (1) EP2069481A4 (https=)
JP (1) JP2010506591A (https=)
KR (1) KR20090094812A (https=)
CN (1) CN101535338A (https=)
AU (1) AU2007333010A1 (https=)
BR (1) BRPI0718389A2 (https=)
CA (1) CA2665678A1 (https=)
IL (1) IL197917A0 (https=)
MX (1) MX2009004043A (https=)
RU (1) RU2009112104A (https=)
WO (1) WO2008073184A2 (https=)

Cited By (3)

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JP2015530091A (ja) * 2012-09-24 2015-10-15 メディミューン リミテッド 細胞株
JP2020522247A (ja) * 2017-06-02 2020-07-30 アンブルックス, インコーポレイテッドAmbrx, Inc. 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US12565646B2 (en) 2019-06-21 2026-03-03 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

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MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
CN103328648B (zh) * 2010-12-03 2015-11-25 国立大学法人东京大学 具有稳定的二级结构的肽和肽库以及它们的制造方法
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
BR112015022641B1 (pt) * 2013-03-15 2023-02-28 Medimmune Limited Construção de dna e processo de preparação de uma proteína-alvo
CN109295025B (zh) * 2014-09-03 2021-04-02 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110520522A (zh) 2017-02-17 2019-11-29 鲁比厄斯治疗法股份有限公司 功能化红系细胞
JP2020522263A (ja) 2017-06-06 2020-07-30 キンドレッド バイオサイエンシズ インコーポレイテッド 獣医学的使用のためのエリスロポエチンおよびアナログ
JP2021520828A (ja) 2018-04-19 2021-08-26 キンドレッド バイオサイエンシズ インコーポレイテッド 医療用の変異体アスパラギナーゼポリペプチド
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
CN114729334A (zh) * 2019-08-08 2022-07-08 布里克生物公司 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系
KR20220080101A (ko) * 2019-10-15 2022-06-14 트러스티스 오브 보스톤 칼리지 향상된 비천연 아미노산 혼입을 위한 키메라 열안정성 아미노아실-tRNA 합성효소
US20230069218A1 (en) * 2019-12-12 2023-03-02 Chugai Seiyaku Kabushiki Kaisha Method for producing peptide containing non-natural amino acid
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697B (zh) * 2024-12-20 2026-03-27 天津大学 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用

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MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
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WO2004039989A1 (ja) * 2002-10-31 2004-05-13 Riken 非天然型アミノ酸組み込みタンパク質の発現方法
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015530091A (ja) * 2012-09-24 2015-10-15 メディミューン リミテッド 細胞株
JP2020522247A (ja) * 2017-06-02 2020-07-30 アンブルックス, インコーポレイテッドAmbrx, Inc. 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP7245786B2 (ja) 2017-06-02 2023-03-24 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP2023075254A (ja) * 2017-06-02 2023-05-30 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US11851662B2 (en) 2017-06-02 2023-12-26 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
JP7617162B2 (ja) 2017-06-02 2025-01-17 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP2025061014A (ja) * 2017-06-02 2025-04-10 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US12577575B2 (en) 2017-06-02 2026-03-17 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
US12565646B2 (en) 2019-06-21 2026-03-03 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

Also Published As

Publication number Publication date
EP2069481A4 (en) 2010-01-06
KR20090094812A (ko) 2009-09-08
IL197917A0 (en) 2011-08-01
WO2008073184A2 (en) 2008-06-19
CA2665678A1 (en) 2008-06-19
EP2069481A2 (en) 2009-06-17
RU2009112104A (ru) 2010-11-27
US20100021963A1 (en) 2010-01-28
BRPI0718389A2 (pt) 2012-04-10
AU2007333010A1 (en) 2008-06-19
MX2009004043A (es) 2009-04-27
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
CN101535338A (zh) 2009-09-16
WO2008073184A3 (en) 2008-11-27

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