JP2010506591A - 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み - Google Patents

哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み Download PDF

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JP2010506591A
JP2010506591A JP2009533372A JP2009533372A JP2010506591A JP 2010506591 A JP2010506591 A JP 2010506591A JP 2009533372 A JP2009533372 A JP 2009533372A JP 2009533372 A JP2009533372 A JP 2009533372A JP 2010506591 A JP2010506591 A JP 2010506591A
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trna
amino acid
unnatural amino
cells
amino acids
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JP2010506591A5 (https=
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ウェンシー・リウ
ピーター・ジー.・シュルツ
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Scripps Research Institute
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
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    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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JP2009533372A 2006-10-18 2007-10-17 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み Pending JP2010506591A (ja)

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US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

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JP2010506591A5 JP2010506591A5 (https=) 2010-12-09

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US (2) US20080166766A1 (https=)
EP (1) EP2069481A4 (https=)
JP (1) JP2010506591A (https=)
KR (1) KR20090094812A (https=)
CN (1) CN101535338A (https=)
AU (1) AU2007333010A1 (https=)
BR (1) BRPI0718389A2 (https=)
CA (1) CA2665678A1 (https=)
IL (1) IL197917A0 (https=)
MX (1) MX2009004043A (https=)
RU (1) RU2009112104A (https=)
WO (1) WO2008073184A2 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015530091A (ja) * 2012-09-24 2015-10-15 メディミューン リミテッド 細胞株
JP2020522247A (ja) * 2017-06-02 2020-07-30 アンブルックス, インコーポレイテッドAmbrx, Inc. 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US12565646B2 (en) 2019-06-21 2026-03-03 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

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MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP1649004A4 (en) * 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
EP2647721B1 (en) * 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
AU2014229999B2 (en) * 2013-03-15 2019-09-12 Medimmune Limited Novel nucleic acid molecules
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN107012121B (zh) * 2016-01-27 2021-01-26 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110520522A (zh) 2017-02-17 2019-11-29 鲁比厄斯治疗法股份有限公司 功能化红系细胞
AU2018281334A1 (en) 2017-06-06 2019-12-19 Kindred Biosciences, Inc. Erythropoietin and analogs for veterinary use
JP2021520828A (ja) 2018-04-19 2021-08-26 キンドレッド バイオサイエンシズ インコーポレイテッド 医療用の変異体アスパラギナーゼポリペプチド
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
US20230279378A1 (en) * 2019-10-15 2023-09-07 Trustees Of Boston College Chimeric thermostable aminoacyl-trna synthetase for enhanced unnatural amino acid incorporation
JP7267458B2 (ja) * 2019-12-12 2023-05-01 中外製薬株式会社 非天然アミノ酸を含むペプチドの製造方法
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697B (zh) * 2024-12-20 2026-03-27 天津大学 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用

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JP4444113B2 (ja) 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015530091A (ja) * 2012-09-24 2015-10-15 メディミューン リミテッド 細胞株
JP2020522247A (ja) * 2017-06-02 2020-07-30 アンブルックス, インコーポレイテッドAmbrx, Inc. 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP7245786B2 (ja) 2017-06-02 2023-03-24 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP2023075254A (ja) * 2017-06-02 2023-05-30 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US11851662B2 (en) 2017-06-02 2023-12-26 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
JP7617162B2 (ja) 2017-06-02 2025-01-17 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
JP2025061014A (ja) * 2017-06-02 2025-04-10 アンブルックス,インコーポレイテッド 非天然アミノ酸含有タンパク質の産生を促進する方法および組成物
US12577575B2 (en) 2017-06-02 2026-03-17 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
US12565646B2 (en) 2019-06-21 2026-03-03 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

Also Published As

Publication number Publication date
US20100021963A1 (en) 2010-01-28
EP2069481A2 (en) 2009-06-17
CN101535338A (zh) 2009-09-16
BRPI0718389A2 (pt) 2012-04-10
RU2009112104A (ru) 2010-11-27
MX2009004043A (es) 2009-04-27
AU2007333010A1 (en) 2008-06-19
KR20090094812A (ko) 2009-09-08
IL197917A0 (en) 2011-08-01
WO2008073184A3 (en) 2008-11-27
EP2069481A4 (en) 2010-01-06
CA2665678A1 (en) 2008-06-19
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
WO2008073184A2 (en) 2008-06-19

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