RU2004120769A - Олигонуклеотид, контролирующий область гибридизации, и его применение - Google Patents
Олигонуклеотид, контролирующий область гибридизации, и его применение Download PDFInfo
- Publication number
- RU2004120769A RU2004120769A RU2004120769/13A RU2004120769A RU2004120769A RU 2004120769 A RU2004120769 A RU 2004120769A RU 2004120769/13 A RU2004120769/13 A RU 2004120769/13A RU 2004120769 A RU2004120769 A RU 2004120769A RU 2004120769 A RU2004120769 A RU 2004120769A
- Authority
- RU
- Russia
- Prior art keywords
- oligonucleotide
- nucleotide sequence
- hybridization
- hybridizing region
- specified
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title claims 60
- 238000009396 hybridization Methods 0.000 title claims 23
- 125000003729 nucleotide group Chemical group 0.000 claims 56
- 239000002773 nucleotide Substances 0.000 claims 55
- 238000000034 method Methods 0.000 claims 23
- 108020004707 nucleic acids Proteins 0.000 claims 15
- 150000007523 nucleic acids Chemical class 0.000 claims 15
- 102000039446 nucleic acids Human genes 0.000 claims 15
- 230000000295 complement effect Effects 0.000 claims 8
- 230000001105 regulatory effect Effects 0.000 claims 5
- -1 2'-OMe-inosine Chemical compound 0.000 claims 4
- 229960003786 inosine Drugs 0.000 claims 3
- WETFHJRYOTYZFD-YIZRAAEISA-N (2r,3s,5s)-2-(hydroxymethyl)-5-(3-nitropyrrol-1-yl)oxolan-3-ol Chemical compound C1[C@H](O)[C@@H](CO)O[C@@H]1N1C=C([N+]([O-])=O)C=C1 WETFHJRYOTYZFD-YIZRAAEISA-N 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 claims 2
- OZFPSOBLQZPIAV-UHFFFAOYSA-N 5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC=CC2=C1 OZFPSOBLQZPIAV-UHFFFAOYSA-N 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 2
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims 2
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- NZJKEQFPRPAEPO-UHFFFAOYSA-N 1h-benzimidazol-4-amine Chemical compound NC1=CC=CC2=C1N=CN2 NZJKEQFPRPAEPO-UHFFFAOYSA-N 0.000 claims 1
- LOJNBPNACKZWAI-UHFFFAOYSA-N 3-nitro-1h-pyrrole Chemical compound [O-][N+](=O)C=1C=CNC=1 LOJNBPNACKZWAI-UHFFFAOYSA-N 0.000 claims 1
- FRYOZNAQLGCIKS-UHFFFAOYSA-N 4-(3-nitro-1h-pyrrol-2-yl)morpholine Chemical compound C1=CNC(N2CCOCC2)=C1[N+](=O)[O-] FRYOZNAQLGCIKS-UHFFFAOYSA-N 0.000 claims 1
- QTJTWXGFPBTKRR-UHFFFAOYSA-N 4-(4-nitro-1h-benzimidazol-2-yl)morpholine Chemical compound N=1C=2C([N+](=O)[O-])=CC=CC=2NC=1N1CCOCC1 QTJTWXGFPBTKRR-UHFFFAOYSA-N 0.000 claims 1
- UTHBAKVGHIUWIP-UHFFFAOYSA-N 4-(5-nitro-1h-indol-2-yl)morpholine Chemical compound C=1C2=CC([N+](=O)[O-])=CC=C2NC=1N1CCOCC1 UTHBAKVGHIUWIP-UHFFFAOYSA-N 0.000 claims 1
- NEJMFSBXFBFELK-UHFFFAOYSA-N 4-nitro-1h-benzimidazole Chemical compound [O-][N+](=O)C1=CC=CC2=C1N=CN2 NEJMFSBXFBFELK-UHFFFAOYSA-N 0.000 claims 1
- QFFLRMDXYQOYKO-KVQBGUIXSA-N 7-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1h-imidazo[4,5-d]triazin-4-one Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NN=NC(O)=C2N=C1 QFFLRMDXYQOYKO-KVQBGUIXSA-N 0.000 claims 1
- LUNAYDDJTHHELV-GIWSHQQXSA-N 9-[(2r,3s,4r,5r)-3-fluoro-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-3h-purin-6-one Chemical compound O[C@]1(F)[C@H](O)[C@@H](CO)O[C@H]1N1C(NC=NC2=O)=C2N=C1 LUNAYDDJTHHELV-GIWSHQQXSA-N 0.000 claims 1
- OXYNAKURPIFROL-PYUPQCDSSA-N 9-[(2s,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)-2-morpholin-4-yloxolan-2-yl]-3h-purin-6-one Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@]1(N1C2=NC=NC(O)=C2N=C1)N1CCOCC1 OXYNAKURPIFROL-PYUPQCDSSA-N 0.000 claims 1
- 208000035473 Communicable disease Diseases 0.000 claims 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 1
- 229930010555 Inosine Natural products 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- JRTXBRPKDAKSSH-UHFFFAOYSA-N aminophosphonic acid;4-nitro-1h-benzimidazole Chemical compound NP(O)(O)=O.[O-][N+](=O)C1=CC=CC2=C1NC=N2 JRTXBRPKDAKSSH-UHFFFAOYSA-N 0.000 claims 1
- ATWWOPGVWCAISQ-UHFFFAOYSA-N aminophosphonic acid;5-nitro-1h-indole Chemical compound NP(O)(O)=O.[O-][N+](=O)C1=CC=C2NC=CC2=C1 ATWWOPGVWCAISQ-UHFFFAOYSA-N 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000013011 mating Effects 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/113—Differential Display Analysis [DDA]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KRPCT/KR01/02133 | 2001-12-08 | ||
| PCT/KR2001/002133 WO2003050304A1 (en) | 2001-12-08 | 2001-12-08 | Annealing control primer system for regulating primer annealing specificity and its applications |
| KRPCT/KR02/00816 | 2002-05-01 | ||
| PCT/KR2002/000816 WO2003093509A1 (en) | 2002-05-01 | 2002-05-01 | Methods and compositions for improving specificity of pcr amplication |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2004120769A true RU2004120769A (ru) | 2005-04-20 |
Family
ID=19198489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2004120769/13A RU2004120769A (ru) | 2001-12-08 | 2002-11-04 | Олигонуклеотид, контролирующий область гибридизации, и его применение |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20030152925A1 (https=) |
| JP (2) | JP4263612B2 (https=) |
| KR (1) | KR100649165B1 (https=) |
| AU (1) | AU2002222737A1 (https=) |
| CA (1) | CA2469383A1 (https=) |
| IL (1) | IL162317A0 (https=) |
| NZ (1) | NZ533221A (https=) |
| RU (1) | RU2004120769A (https=) |
| WO (1) | WO2003050304A1 (https=) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280422C (zh) * | 2004-08-26 | 2006-10-18 | 北京博奥生物芯片有限责任公司 | 一种不对称pcr扩增方法及其应用 |
| US20060188910A1 (en) * | 2005-02-18 | 2006-08-24 | Applera Corporation | Compositions, methods, and kits for analyzing DNA methylation |
| US8211899B2 (en) * | 2005-08-30 | 2012-07-03 | Inter-University Research Institute Corporation National Institute Of Natural Sciences | Artificial nucleic acid bases and their use in base pairing natural nucleic acid bases |
| CA2626977A1 (en) * | 2005-10-27 | 2007-05-03 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
| DE602006018352D1 (de) | 2005-12-06 | 2010-12-30 | Ambion Inc | Rückübertragungs-primer und verfahren zu deren entwurf |
| KR100860619B1 (ko) * | 2006-02-23 | 2008-09-29 | 주식회사 씨젠 | 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드 |
| WO2007129778A1 (en) * | 2006-05-04 | 2007-11-15 | Seegene, Inc. | Method for amplifying unknown dna sequence adjacent to known sequence |
| EP3121286B1 (en) | 2006-12-21 | 2019-11-20 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| EP1978104A1 (en) * | 2007-04-03 | 2008-10-08 | Freie Universität Berlin | A method for the quantitative analysis of RNA molecules in a sample |
| KR100987352B1 (ko) | 2008-04-15 | 2010-10-12 | 주식회사 인트론바이오테크놀로지 | 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법 |
| US9534255B2 (en) | 2008-12-17 | 2017-01-03 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| EP3249053A1 (en) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| JP5687414B2 (ja) * | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| EP3597657B1 (en) * | 2009-05-21 | 2021-09-01 | Siemens Healthcare Diagnostics Inc. | Universal tags with non-natural nucleobases |
| EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| EP2475777A4 (en) * | 2009-09-11 | 2013-03-06 | Nugen Technologies Inc | COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS |
| MY157586A (en) * | 2011-09-14 | 2016-06-16 | Ngk Insulators Ltd | Method for detecting target nucleic acid |
| ES2637489T3 (es) | 2012-04-18 | 2017-10-13 | F. Hoffmann-La Roche Ag | Ensayo de VHE |
| KR101525495B1 (ko) * | 2013-11-15 | 2015-06-03 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| KR101723207B1 (ko) * | 2015-01-29 | 2017-04-05 | (주)다이오진 | 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법 |
| CN107075576B (zh) * | 2015-02-25 | 2021-05-25 | 精确诊断有限公司 | 哑铃结构寡核苷酸、包含其的核酸扩增用引物及利用该引物的核酸扩增方法 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| JP6698708B2 (ja) | 2015-06-09 | 2020-05-27 | ライフ テクノロジーズ コーポレーション | 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 |
| KR101961642B1 (ko) * | 2016-04-25 | 2019-03-25 | (주)진매트릭스 | 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 |
| EP3682025A1 (en) * | 2017-09-14 | 2020-07-22 | H. Hoffnabb-La Roche Ag | Novel method for generating circular single-stranded dna libraries |
| KR101856205B1 (ko) * | 2017-11-16 | 2018-06-20 | 제노플랜코리아 주식회사 | 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2544292A (en) * | 1991-09-11 | 1993-04-05 | Medical Research Council | Improvements in oligonucleotide primers and probes |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| WO2001023618A2 (en) * | 1999-09-30 | 2001-04-05 | Qiagen Genomics, Inc. | Compositions and methods for reducing oligonucleotide hybridization and priming specificity |
-
2001
- 2001-12-08 AU AU2002222737A patent/AU2002222737A1/en not_active Abandoned
- 2001-12-08 WO PCT/KR2001/002133 patent/WO2003050304A1/en not_active Ceased
- 2001-12-14 US US10/014,496 patent/US20030152925A1/en not_active Abandoned
-
2002
- 2002-09-19 JP JP2003551326A patent/JP4263612B2/ja not_active Expired - Lifetime
- 2002-09-19 IL IL16231702A patent/IL162317A0/xx unknown
- 2002-09-19 KR KR1020047008441A patent/KR100649165B1/ko not_active Expired - Lifetime
- 2002-11-04 NZ NZ533221A patent/NZ533221A/en unknown
- 2002-11-04 RU RU2004120769/13A patent/RU2004120769A/ru not_active Application Discontinuation
- 2002-11-04 JP JP2003551327A patent/JP2005522190A/ja active Pending
- 2002-11-04 CA CA002469383A patent/CA2469383A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20030152925A1 (en) | 2003-08-14 |
| JP4263612B2 (ja) | 2009-05-13 |
| CA2469383A1 (en) | 2003-06-19 |
| KR20050028904A (ko) | 2005-03-23 |
| KR100649165B1 (ko) | 2006-11-24 |
| AU2002222737A1 (en) | 2003-06-23 |
| JP2005522190A (ja) | 2005-07-28 |
| WO2003050304A1 (en) | 2003-06-19 |
| NZ533221A (en) | 2006-01-27 |
| IL162317A0 (en) | 2005-11-20 |
| JP2005511096A (ja) | 2005-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20070124 |