JP2009521223A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2009521223A5 JP2009521223A5 JP2008547582A JP2008547582A JP2009521223A5 JP 2009521223 A5 JP2009521223 A5 JP 2009521223A5 JP 2008547582 A JP2008547582 A JP 2008547582A JP 2008547582 A JP2008547582 A JP 2008547582A JP 2009521223 A5 JP2009521223 A5 JP 2009521223A5
- Authority
- JP
- Japan
- Prior art keywords
- reversible terminator
- nucleic acid
- terminator nucleotide
- modified reversible
- template nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002773 nucleotide Substances 0.000 claims 33
- 125000003729 nucleotide group Chemical group 0.000 claims 33
- 230000002441 reversible effect Effects 0.000 claims 31
- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 102000039446 nucleic acids Human genes 0.000 claims 21
- 108020004707 nucleic acids Proteins 0.000 claims 21
- 238000000034 method Methods 0.000 claims 14
- 230000004048 modification Effects 0.000 claims 11
- 238000012986 modification Methods 0.000 claims 11
- 229910019142 PO4 Inorganic materials 0.000 claims 7
- 239000010452 phosphate Substances 0.000 claims 7
- 238000006243 chemical reaction Methods 0.000 claims 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 6
- 239000011541 reaction mixture Substances 0.000 claims 6
- 238000012163 sequencing technique Methods 0.000 claims 6
- 125000006239 protecting group Chemical group 0.000 claims 5
- 230000015572 biosynthetic process Effects 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 238000003786 synthesis reaction Methods 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 238000003205 genotyping method Methods 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 1
- 235000011180 diphosphates Nutrition 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75282705P | 2005-12-21 | 2005-12-21 | |
| US60/752,827 | 2005-12-21 | ||
| US84404106P | 2006-09-11 | 2006-09-11 | |
| US60/844,041 | 2006-09-11 | ||
| PCT/US2006/048933 WO2007075967A2 (en) | 2005-12-21 | 2006-12-20 | Sequencing and genotyping using reversibly 2'-modified nucleotides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009521223A JP2009521223A (ja) | 2009-06-04 |
| JP2009521223A5 true JP2009521223A5 (https=) | 2010-02-25 |
| JP5368108B2 JP5368108B2 (ja) | 2013-12-18 |
Family
ID=38218645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008547582A Active JP5368108B2 (ja) | 2005-12-21 | 2006-12-20 | 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9206476B2 (https=) |
| EP (1) | EP1974057B1 (https=) |
| JP (1) | JP5368108B2 (https=) |
| CN (1) | CN101346472B (https=) |
| CA (1) | CA2631316C (https=) |
| ES (1) | ES2388722T3 (https=) |
| WO (1) | WO2007075967A2 (https=) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7928207B2 (en) * | 2004-06-28 | 2011-04-19 | Roche Molecular Systems, Inc | Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides |
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US8212020B2 (en) * | 2005-03-11 | 2012-07-03 | Steven Albert Benner | Reagents for reversibly terminating primer extension |
| CN101346472B (zh) | 2005-12-21 | 2013-04-24 | 霍夫曼—拉罗奇有限公司 | 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 |
| US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| US8703422B2 (en) * | 2007-06-06 | 2014-04-22 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| US8182993B2 (en) * | 2007-06-06 | 2012-05-22 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| WO2013154750A1 (en) | 2012-04-10 | 2013-10-17 | The Trustees Of Columbia Unversity In The City Of New York | Systems and methods for biological ion channel interfaces |
| US20100261185A1 (en) | 2009-03-27 | 2010-10-14 | Life Technologies Corporation | Labeled enzyme compositions, methods and systems |
| EP2427572B1 (en) * | 2009-05-01 | 2013-08-28 | Illumina, Inc. | Sequencing methods |
| WO2010141391A2 (en) | 2009-06-05 | 2010-12-09 | Life Technologies Corporation | Mutant dna polymerases |
| US20100330556A1 (en) * | 2009-06-30 | 2010-12-30 | Brian Jon Peter | Genome analysis using a nicking endonuclease |
| WO2011011175A2 (en) | 2009-07-24 | 2011-01-27 | Illumina, Inc. | Method for sequencing a polynucleotide template |
| US8965076B2 (en) | 2010-01-13 | 2015-02-24 | Illumina, Inc. | Data processing system and methods |
| CN103620403B (zh) * | 2011-01-11 | 2016-08-10 | 纽约市哥伦比亚大学理事会 | 使用纳米管监测单分子的系统和方法 |
| WO2012116161A1 (en) | 2011-02-23 | 2012-08-30 | The Trustees Of Columbia University In The City Of New York | Systems and methods for single-molecule detection using nanopores |
| US10378051B2 (en) * | 2011-09-29 | 2019-08-13 | Illumina Cambridge Limited | Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing |
| WO2013154999A2 (en) * | 2012-04-09 | 2013-10-17 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| WO2013158280A1 (en) | 2012-04-20 | 2013-10-24 | The Trustees Of Columbia University In The City Of New York | Systems and methods for single-molecule nucleic-acid assay platforms |
| WO2014034275A1 (ja) * | 2012-08-30 | 2014-03-06 | 株式会社 日立ハイテクノロジーズ | 核酸分析装置 |
| US9650406B2 (en) | 2013-02-28 | 2017-05-16 | Centrillion Technology Holdings Corporation | Reversible terminator molecules and methods of their use |
| US9146248B2 (en) | 2013-03-14 | 2015-09-29 | Intelligent Bio-Systems, Inc. | Apparatus and methods for purging flow cells in nucleic acid sequencing instruments |
| ES2713653T3 (es) | 2013-03-15 | 2019-05-23 | Ibis Biosciences Inc | Análogos nucleotídicos para secuenciación |
| US9591268B2 (en) | 2013-03-15 | 2017-03-07 | Qiagen Waltham, Inc. | Flow cell alignment methods and systems |
| WO2016182984A1 (en) | 2015-05-08 | 2016-11-17 | Centrillion Technology Holdings Corporation | Disulfide-linked reversible terminators |
| KR102254451B1 (ko) | 2016-04-22 | 2021-05-20 | 옴니옴 인코포레이티드 | 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템 |
| BR112018073378A2 (pt) * | 2016-05-20 | 2019-03-06 | Quantum-Si Incorporated | composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos |
| CA3029320C (en) * | 2016-06-24 | 2022-08-09 | The Regents Of The University Of California | Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates |
| CN108239669B (zh) * | 2016-12-23 | 2021-03-16 | 深圳华大智造科技股份有限公司 | 检测可逆性阻断dNTP中非阻断杂质含量的方法和试剂盒 |
| US10975427B2 (en) | 2017-01-20 | 2021-04-13 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
| EP3625240A4 (en) | 2017-05-18 | 2021-01-27 | Ultima Genomics, Inc. | SEQUENCING WITH NON-NATURAL NUCLEOTIDES |
| CA3069985A1 (en) | 2017-07-24 | 2019-01-31 | Quantum-Si Incorporated | High intensity labeled reactant compositions and methods for sequencing |
| WO2019099420A1 (en) * | 2017-11-15 | 2019-05-23 | Yan Wang | A method for detecting multiple dna mutations and copy number variations |
| KR20200139671A (ko) * | 2018-02-06 | 2020-12-14 | 옴니옴 인코포레이티드 | 핵산 프라이머 연장을 위한 조성물 및 기술 |
| AU2019300169A1 (en) | 2018-07-13 | 2021-01-28 | Quantum-Si Incorporated | Biconjugatable labels and methods of use |
| EP3856753A4 (en) | 2018-09-28 | 2022-11-16 | Centrillion Technology Holdings Corporation | DISULFIDE CROSSLINKED REVERSIBLE TERMINATORS |
| MX2021008867A (es) | 2019-01-23 | 2021-08-19 | Quantum Si Inc | Composiciones reactantes marcadas de intensidad alta y metodos de secuenciamiento. |
| JP2023518105A (ja) * | 2019-12-30 | 2023-04-27 | 源點生物科技股▲フン▼有限公司 | 酵素を使用して核酸配列を調製するための方法 |
| US20230037041A1 (en) * | 2019-12-30 | 2023-02-02 | Yuandian Biolabs Co., Ltd. | Apparatus and method for preparing nucleic acid sequences using enzyme |
| US20230129228A1 (en) * | 2020-03-18 | 2023-04-27 | Mgi Tech Co. Ltd. | Restoring phase in massively parallel sequencing |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5302509A (en) * | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| US5436143A (en) * | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
| US5532130A (en) | 1993-07-20 | 1996-07-02 | Dyad Pharmaceutical Corporation | Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides |
| AU3174600A (en) * | 1999-03-10 | 2000-09-28 | Asm Scientific, Inc. | A method for direct nucleic acid sequencing |
| US7572581B2 (en) * | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7947817B2 (en) * | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| AU2005250239A1 (en) * | 2004-06-02 | 2005-12-15 | Alere San Diego, Inc. | 2'-nitrobenzyl-modified ribonucleotides |
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| CN101346472B (zh) | 2005-12-21 | 2013-04-24 | 霍夫曼—拉罗奇有限公司 | 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 |
| EP1987944B1 (de) | 2007-05-04 | 2012-07-25 | Leister Technologies AG | Laserdurchstrahlschweissvorrichtung und Verfahren zum Verbinden von Kunststoffwerkstücken |
-
2006
- 2006-12-20 CN CN2006800488413A patent/CN101346472B/zh active Active
- 2006-12-20 ES ES06847988T patent/ES2388722T3/es active Active
- 2006-12-20 WO PCT/US2006/048933 patent/WO2007075967A2/en not_active Ceased
- 2006-12-20 EP EP06847988A patent/EP1974057B1/en active Active
- 2006-12-20 CA CA2631316A patent/CA2631316C/en not_active Expired - Fee Related
- 2006-12-20 US US12/158,257 patent/US9206476B2/en active Active
- 2006-12-20 JP JP2008547582A patent/JP5368108B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2009521223A5 (https=) | ||
| US8329394B2 (en) | Methods and substances for isolation and detection of small polynucleotides | |
| EP0359789B1 (en) | Amplification and detection of nucleic acid sequences | |
| US20110003301A1 (en) | Methods for detecting genetic variations in dna samples | |
| EP1322782B1 (en) | Method of nucleic acid typing or sequencing | |
| CA2692633C (en) | Method for the simultaneous detection of multiple nucleic acid sequences in a sample | |
| CA2528577A1 (en) | Methods and compositions for whole genome amplification and genotyping | |
| WO2013106737A1 (en) | Genotyping by next-generation sequencing | |
| WO2007075967B1 (en) | Sequencing and genotyping using reversibly 2'-modified nucleotides | |
| RU2012129362A (ru) | Обнаружение мишени tsg праймером | |
| JP2003009890A (ja) | 高処理能多型スクリーニング | |
| CA2582809A1 (en) | Replica amplification of nucleic acid arrays | |
| KR20070011354A (ko) | 취약 ｘ염색체 증후군과 같은 ｓｔｒｐ의 검출 방법 | |
| JP2013509871A5 (https=) | ||
| JP6126381B2 (ja) | 標的核酸の検出方法及びキット | |
| JP2009529337A5 (https=) | ||
| US20090023151A1 (en) | Method For The Labeling And Detection Of Small Polynucleotides | |
| JP2022533228A (ja) | 光によりトリガーされる核酸構築物および分子検出のための方法 | |
| JP2020092721A5 (https=) | ||
| CN115948604A (zh) | 一种基于DNAzyme与PER扩增的病毒检测试剂盒 | |
| RU2010154139A (ru) | Способ обнаружения респираторных вирусных агентов в образце | |
| JP5319148B2 (ja) | 標的核酸中の変異の検出方法及びアレイ | |
| EP3828283A1 (en) | An improved sequencing method and kit | |
| RU2006138865A (ru) | Анализы на основе нуклеиновой кислоты для идентификации полиморфизмов fc-рецептора | |
| EP1616027A4 (en) | DETECTION OF TARGET NUCLEIC ACID BY MEANS OF POLYMERIC ACTION AND ENZYMATIC DETECTION OF UNLOCKED PYROPHOSPHATE |