JP5368108B2 - 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 - Google Patents

可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 Download PDF

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JP5368108B2
JP5368108B2 JP2008547582A JP2008547582A JP5368108B2 JP 5368108 B2 JP5368108 B2 JP 5368108B2 JP 2008547582 A JP2008547582 A JP 2008547582A JP 2008547582 A JP2008547582 A JP 2008547582A JP 5368108 B2 JP5368108 B2 JP 5368108B2
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reversible terminator
nucleic acid
modified
nucleotide
terminator nucleotide
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JP2009521223A (ja
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エイチ． ジェルファンド，デイビッド
ピー． グプタ，エイマー
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
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JP2008547582A 2005-12-21 2006-12-20 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法 Active JP5368108B2 (ja)

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Application Number Priority Date Filing Date Title
US75282705P 2005-12-21 2005-12-21
US60/752,827 2005-12-21
US84404106P 2006-09-11 2006-09-11
US60/844,041 2006-09-11
PCT/US2006/048933 WO2007075967A2 (en) 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides

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JP2009521223A JP2009521223A (ja) 2009-06-04
JP2009521223A5 JP2009521223A5 (https=) 2010-02-25
JP5368108B2 true JP5368108B2 (ja) 2013-12-18

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JP2008547582A Active JP5368108B2 (ja) 2005-12-21 2006-12-20 可逆的停止ヌクレオチドを利用する配列決定法および遺伝型解析法

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US (1) US9206476B2 (https=)
EP (1) EP1974057B1 (https=)
JP (1) JP5368108B2 (https=)
CN (1) CN101346472B (https=)
CA (1) CA2631316C (https=)
ES (1) ES2388722T3 (https=)
WO (1) WO2007075967A2 (https=)

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US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8703422B2 (en) * 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8182993B2 (en) * 2007-06-06 2012-05-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2013154750A1 (en) 2012-04-10 2013-10-17 The Trustees Of Columbia Unversity In The City Of New York Systems and methods for biological ion channel interfaces
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
EP2427572B1 (en) * 2009-05-01 2013-08-28 Illumina, Inc. Sequencing methods
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
WO2011011175A2 (en) 2009-07-24 2011-01-27 Illumina, Inc. Method for sequencing a polynucleotide template
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
CN103620403B (zh) * 2011-01-11 2016-08-10 纽约市哥伦比亚大学理事会 使用纳米管监测单分子的系统和方法
WO2012116161A1 (en) 2011-02-23 2012-08-30 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule detection using nanopores
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
WO2013154999A2 (en) * 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
WO2013158280A1 (en) 2012-04-20 2013-10-24 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule nucleic-acid assay platforms
WO2014034275A1 (ja) * 2012-08-30 2014-03-06 株式会社 日立ハイテクノロジーズ 核酸分析装置
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
ES2713653T3 (es) 2013-03-15 2019-05-23 Ibis Biosciences Inc Análogos nucleotídicos para secuenciación
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
WO2016182984A1 (en) 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
KR102254451B1 (ko) 2016-04-22 2021-05-20 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
BR112018073378A2 (pt) * 2016-05-20 2019-03-06 Quantum-Si Incorporated composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos
CA3029320C (en) * 2016-06-24 2022-08-09 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
CN108239669B (zh) * 2016-12-23 2021-03-16 深圳华大智造科技股份有限公司 检测可逆性阻断dNTP中非阻断杂质含量的方法和试剂盒
US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
EP3625240A4 (en) 2017-05-18 2021-01-27 Ultima Genomics, Inc. SEQUENCING WITH NON-NATURAL NUCLEOTIDES
CA3069985A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
KR20200139671A (ko) * 2018-02-06 2020-12-14 옴니옴 인코포레이티드 핵산 프라이머 연장을 위한 조성물 및 기술
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
EP3856753A4 (en) 2018-09-28 2022-11-16 Centrillion Technology Holdings Corporation DISULFIDE CROSSLINKED REVERSIBLE TERMINATORS
MX2021008867A (es) 2019-01-23 2021-08-19 Quantum Si Inc Composiciones reactantes marcadas de intensidad alta y metodos de secuenciamiento.
JP2023518105A (ja) * 2019-12-30 2023-04-27 源點生物科技股▲フン▼有限公司 酵素を使用して核酸配列を調製するための方法
US20230037041A1 (en) * 2019-12-30 2023-02-02 Yuandian Biolabs Co., Ltd. Apparatus and method for preparing nucleic acid sequences using enzyme
US20230129228A1 (en) * 2020-03-18 2023-04-27 Mgi Tech Co. Ltd. Restoring phase in massively parallel sequencing

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US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5532130A (en) 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
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US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
AU2005250239A1 (en) * 2004-06-02 2005-12-15 Alere San Diego, Inc. 2'-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
EP1987944B1 (de) 2007-05-04 2012-07-25 Leister Technologies AG Laserdurchstrahlschweissvorrichtung und Verfahren zum Verbinden von Kunststoffwerkstücken

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EP1974057A2 (en) 2008-10-01
WO2007075967A2 (en) 2007-07-05
US9206476B2 (en) 2015-12-08
CA2631316A1 (en) 2007-07-05
CN101346472B (zh) 2013-04-24
HK1124640A1 (en) 2009-07-17
JP2009521223A (ja) 2009-06-04
WO2007075967A3 (en) 2008-01-03
CN101346472A (zh) 2009-01-14
ES2388722T3 (es) 2012-10-18
US20080293071A1 (en) 2008-11-27
CA2631316C (en) 2012-12-04
WO2007075967B1 (en) 2008-02-21
EP1974057B1 (en) 2012-06-13

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