CA2631316C - Sequencing and genotyping using reversibly 2'-modified nucleotides - Google Patents

Sequencing and genotyping using reversibly 2'-modified nucleotides Download PDF

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Publication number
CA2631316C
CA2631316C CA2631316A CA2631316A CA2631316C CA 2631316 C CA2631316 C CA 2631316C CA 2631316 A CA2631316 A CA 2631316A CA 2631316 A CA2631316 A CA 2631316A CA 2631316 C CA2631316 C CA 2631316C
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CA
Canada
Prior art keywords
reversible terminator
terminator nucleotide
phosphate
nucleic acid
nucleotide
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2631316A
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English (en)
French (fr)
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CA2631316A1 (en
Inventor
David H. Gelfand
Amar P. Gupta
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2631316A1 publication Critical patent/CA2631316A1/en
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Publication of CA2631316C publication Critical patent/CA2631316C/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA2631316A 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides Expired - Fee Related CA2631316C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US75282705P 2005-12-21 2005-12-21
US60/752,827 2005-12-21
US84404106P 2006-09-11 2006-09-11
US60/844,041 2006-09-11
PCT/US2006/048933 WO2007075967A2 (en) 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides

Publications (2)

Publication Number Publication Date
CA2631316A1 CA2631316A1 (en) 2007-07-05
CA2631316C true CA2631316C (en) 2012-12-04

Family

ID=38218645

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2631316A Expired - Fee Related CA2631316C (en) 2005-12-21 2006-12-20 Sequencing and genotyping using reversibly 2'-modified nucleotides

Country Status (7)

Country Link
US (1) US9206476B2 (https=)
EP (1) EP1974057B1 (https=)
JP (1) JP5368108B2 (https=)
CN (1) CN101346472B (https=)
CA (1) CA2631316C (https=)
ES (1) ES2388722T3 (https=)
WO (1) WO2007075967A2 (https=)

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US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7928207B2 (en) 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
ES2388722T3 (es) 2005-12-21 2012-10-18 F. Hoffmann-La Roche Ag Secuenciación y genotipado utilizando nucleótidos modificados en 2' de forma reversible
WO2008042067A2 (en) * 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8703422B2 (en) * 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CA2689626C (en) * 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2013154750A1 (en) 2012-04-10 2013-10-17 The Trustees Of Columbia Unversity In The City Of New York Systems and methods for biological ion channel interfaces
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
WO2010127304A2 (en) * 2009-05-01 2010-11-04 Illumina, Inc. Sequencing methods
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
EP2456892B1 (en) 2009-07-24 2014-10-01 Illumina, Inc. Method for sequencing a polynucleotide template
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
CN103620403B (zh) 2011-01-11 2016-08-10 纽约市哥伦比亚大学理事会 使用纳米管监测单分子的系统和方法
EP2678669A1 (en) 2011-02-23 2014-01-01 The Trustees of Columbia University in the City of New York Systems and methods for single-molecule detection using nanopores
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
WO2013154999A2 (en) * 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
WO2013158280A1 (en) 2012-04-20 2013-10-24 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule nucleic-acid assay platforms
JPWO2014034275A1 (ja) * 2012-08-30 2016-08-08 株式会社日立ハイテクノロジーズ 核酸分析装置
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US10428379B2 (en) 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
EP3091026B1 (en) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
KR102254451B1 (ko) 2016-04-22 2021-05-20 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
EP3458465B1 (en) * 2016-05-20 2024-05-08 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
WO2017223517A1 (en) * 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
CN108239669B (zh) * 2016-12-23 2021-03-16 深圳华大智造科技股份有限公司 检测可逆性阻断dNTP中非阻断杂质含量的方法和试剂盒
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
EP3625240A4 (en) 2017-05-18 2021-01-27 Ultima Genomics, Inc. SEQUENCING WITH NON-NATURAL NUCLEOTIDES
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
JP2021512648A (ja) * 2018-02-06 2021-05-20 オムニオム・インコーポレイテッド 核酸プライマー伸長のための組成物および技術
CA3105715A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
WO2020154546A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
KR20220104057A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 장치 및 방법
US20220356500A1 (en) * 2019-12-30 2022-11-10 Yuandian Biolabs Co., Ltd. Method for preparing nucleic acid sequences using enzyme
CN115427586B (zh) * 2020-03-18 2025-03-28 深圳华大智造科技股份有限公司 大规模平行测序中的恢复阶段

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
WO2000053805A1 (en) * 1999-03-10 2000-09-14 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
AU2005250239A1 (en) * 2004-06-02 2005-12-15 Alere San Diego, Inc. 2'-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
ES2388722T3 (es) 2005-12-21 2012-10-18 F. Hoffmann-La Roche Ag Secuenciación y genotipado utilizando nucleótidos modificados en 2' de forma reversible
EP1987944B1 (de) 2007-05-04 2012-07-25 Leister Technologies AG Laserdurchstrahlschweissvorrichtung und Verfahren zum Verbinden von Kunststoffwerkstücken

Also Published As

Publication number Publication date
WO2007075967A3 (en) 2008-01-03
EP1974057B1 (en) 2012-06-13
HK1124640A1 (en) 2009-07-17
JP5368108B2 (ja) 2013-12-18
ES2388722T3 (es) 2012-10-18
CA2631316A1 (en) 2007-07-05
WO2007075967B1 (en) 2008-02-21
US20080293071A1 (en) 2008-11-27
CN101346472B (zh) 2013-04-24
CN101346472A (zh) 2009-01-14
JP2009521223A (ja) 2009-06-04
EP1974057A2 (en) 2008-10-01
WO2007075967A2 (en) 2007-07-05
US9206476B2 (en) 2015-12-08

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