PE20110903A1 - Metodo de purificacion industrial de ficotoxinas biologicamente activas - Google Patents
Metodo de purificacion industrial de ficotoxinas biologicamente activasInfo
- Publication number
- PE20110903A1 PE20110903A1 PE2011001285A PE2011001285A PE20110903A1 PE 20110903 A1 PE20110903 A1 PE 20110903A1 PE 2011001285 A PE2011001285 A PE 2011001285A PE 2011001285 A PE2011001285 A PE 2011001285A PE 20110903 A1 PE20110903 A1 PE 20110903A1
- Authority
- PE
- Peru
- Prior art keywords
- phycotoxins
- ciacelias
- gonyaulatoxin
- neosaxitoxin
- saxitoxin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/12—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains three hetero rings
- C07D487/14—Ortho-condensed systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P23/00—Anaesthetics
- A61P23/02—Local anaesthetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/04—Centrally acting analgesics, e.g. opioids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/12—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains three hetero rings
- C07D487/16—Peri-condensed systems
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Neurology (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Pain & Pain Management (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Epidemiology (AREA)
- Neurosurgery (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cephalosporin Compounds (AREA)
Abstract
SE REFIERE A UN METODO DE PURIFICACION INDUSTRIAL DE FICOTOXINAS BIOLOGICAMENTE ACTIVAS TALES COMO NEOSAXITOXINA, SAXITOXINA Y GONYAULATOXINA, QUE COMPRENDE: A) PROPORCIONAR UNA CANTIDAD DE UNA FUENTE DE FICOTOXINAS TAL COMO CULTIVO DE UN CLON DE CIANOBACTERIAS, UN PELLET HUMEDO O CONGELADO DE CIANOBACTERIAS, ENTRE OTROS; B) EL MATERIAL PROVENIENTE DE LA LISIS DE CIANOBACTERIAS, SE SOMETE A UNA EXTRACCION EN FRIO Y SEPARACION DE FASES ORGANICA Y ACUOSA (MEZCLA CLOROFORMO:METANOL 1:1 EN 50% Y ACIDO ACETICO 1mM EN 50% V/V CON PH ENTRE 4 Y 5) SIENDO EL MEDIO ACIDO PARA MANTENER LA ACTIVIDAD BIOLOGICA DEL PRINCIPIO ACTIVO; C) OBTENER UN CONCENTRADO DE LA FASE ACUOSA OBTENIDA EN LA ETAPA ANTERIOR O EL MEDIO DE CULTIVO DE LA ETAPA (A) USANDO UN ROTOR VAPORIZADOR A TEMPERATURA AMBIENTE; D) CENTRIFUGAR EL CONCENTRADO PARA OBTENER UN SOBRENADANTE; E) PASAR EL SOBRENADANTE POR UNA COLUMNA DE TIERRAS DE DIATOMEAS, LAVAR LA COLUMNA PARA OBTENER UN ELUIDO DE FICOTOXINAS CON UNA SOLUCION DE ETANOL:AGUA:ACIDO ACETICO 5mM 2:1:1; F) PASAR EL ELUIDO OBTENIDO POR COLUMNAS DE CARBON ACTIVADO Y SE ELUYEN LAS FICOTOXINAS; G) PASAR EL ELUIDO ANTERIOR POR UNA COLUMNA DE TIERRA DE DIATOMEAS Y SE ELUYEN LAS FICOTOXINAS; H) DEJAR EN FASE ACUOSA EL ELUIDO ANTERIOR EVAPORANDO LOS SOLVENTES ORGANICOS OBTENIENDOSE UN EXTRACTO DE FICOTOXINA PARCIALMENTE PURIFICADO; I) SOMETER A HPLC PREPARATIVA EL EXTRACTO PURIFICADO OBTENIENDO LA FICOTOXINA PURA. DICHAS FICOTOXINAS SON DE APLICACION COSMETICA
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2009000723A CL2009000723A1 (es) | 2009-03-24 | 2009-03-24 | Metodo de purificacion industrial de ficotoxinas biologicamente activas que comprende proporcionar una cantidad adecuada de una fuente de ficotoxinas como por ejemplo el cultivo de un clon de cianobacterias. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20110903A1 true PE20110903A1 (es) | 2012-01-19 |
Family
ID=42780210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2011001285A PE20110903A1 (es) | 2009-03-24 | 2010-03-18 | Metodo de purificacion industrial de ficotoxinas biologicamente activas |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2412714B9 (es) |
| JP (1) | JP5728774B2 (es) |
| KR (1) | KR20110130420A (es) |
| AR (1) | AR075931A1 (es) |
| BR (1) | BRPI1013548B8 (es) |
| CA (1) | CA2754154C (es) |
| CL (1) | CL2009000723A1 (es) |
| DK (1) | DK2412714T5 (es) |
| ES (1) | ES2562637T3 (es) |
| HR (1) | HRP20160079T1 (es) |
| HU (1) | HUE027480T2 (es) |
| MX (1) | MX2011010005A (es) |
| PE (1) | PE20110903A1 (es) |
| WO (1) | WO2010109386A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8957207B2 (en) | 2009-03-24 | 2015-02-17 | Proteus S.A. | Methods for producing phycotoxins |
| EP2968225B1 (en) | 2013-03-15 | 2019-02-20 | The Children's Medical Center Corporation | Neosaxitoxin combination formulations for prolonged local anesthesia |
| US9902625B2 (en) | 2014-09-03 | 2018-02-27 | International Business Machines Corporation | Removal of HAB-produced toxins from bodies of water |
| GB201602576D0 (en) | 2016-02-12 | 2016-03-30 | Bergen Teknologioverforing As | Process |
| CN107602580B (zh) * | 2017-09-08 | 2019-06-11 | 浙江理工大学 | 一种聚酮类化合物及其制备方法和应用 |
| CN109142595A (zh) * | 2018-08-23 | 2019-01-04 | 中国水产科学研究院黄海水产研究所 | 一种麻痹性贝类毒素标准溶液的制备方法 |
| CA3113727A1 (en) * | 2018-09-21 | 2020-03-26 | The Cawthron Institute Trust Board | A semisynthetic method of preparing neosaxitoxin |
| EP3853231A4 (en) * | 2018-09-21 | 2022-05-11 | The Cawthron Institute Trust Board | PREPARATIVE SCALE CONVERSION OF GONYAUTOXINS TO NEOSAXITOXIN |
| CN114657222A (zh) * | 2022-04-08 | 2022-06-24 | 湖北省水利水电科学研究院 | 一种从水华束丝藻中提取类菌胞素氨基酸的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4001413A (en) | 1973-06-12 | 1977-01-04 | Astra Pharmaceutical Products, Inc. | Pharmaceutical local anesthetic composition employing saxitoxin |
| US6030974A (en) * | 1997-04-02 | 2000-02-29 | The Regents Of The University Of California | Method of anesthesia |
| ES2401570T3 (es) * | 2001-11-15 | 2013-04-22 | Phytotox Limited | Composiciones farmacéuticas que contienen 3,4-propinoperhidropurinas y usos de las mismas para bloquear la transmisión neuronal |
| CA2607589A1 (en) * | 2004-05-07 | 2005-11-24 | Phytotox Limited | Methods of treating wounds with gonyautoxins |
| DE102006028817A1 (de) * | 2006-06-21 | 2007-12-27 | Evonik Degussa Gmbh | Aufarbeitung von Reaktionslösungen aus Ganzzell-Biotransformationen |
-
2009
- 2009-03-24 CL CL2009000723A patent/CL2009000723A1/es unknown
-
2010
- 2010-03-18 BR BRPI1013548A patent/BRPI1013548B8/pt active IP Right Grant
- 2010-03-18 HU HUE10755518A patent/HUE027480T2/en unknown
- 2010-03-18 CA CA2754154A patent/CA2754154C/en active Active
- 2010-03-18 ES ES10755518.7T patent/ES2562637T3/es active Active
- 2010-03-18 MX MX2011010005A patent/MX2011010005A/es active IP Right Grant
- 2010-03-18 WO PCT/IB2010/051187 patent/WO2010109386A1/es active Application Filing
- 2010-03-18 KR KR1020117021288A patent/KR20110130420A/ko active Search and Examination
- 2010-03-18 JP JP2012501444A patent/JP5728774B2/ja active Active
- 2010-03-18 DK DK10755518.7T patent/DK2412714T5/en active
- 2010-03-18 PE PE2011001285A patent/PE20110903A1/es active IP Right Grant
- 2010-03-18 EP EP10755518.7A patent/EP2412714B9/en active Active
- 2010-03-23 AR ARP100100934A patent/AR075931A1/es active IP Right Grant
-
2016
- 2016-01-25 HR HRP20160079TT patent/HRP20160079T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI1013548B8 (pt) | 2021-05-25 |
| ES2562637T9 (es) | 2017-05-30 |
| HRP20160079T1 (hr) | 2016-04-08 |
| CA2754154C (en) | 2013-12-17 |
| EP2412714B9 (en) | 2016-11-23 |
| EP2412714A1 (en) | 2012-02-01 |
| DK2412714T3 (en) | 2016-02-01 |
| MX2011010005A (es) | 2011-10-10 |
| JP2012521408A (ja) | 2012-09-13 |
| CL2009000723A1 (es) | 2009-06-19 |
| JP5728774B2 (ja) | 2015-06-03 |
| HUE027480T2 (en) | 2016-09-28 |
| EP2412714A4 (en) | 2012-10-10 |
| DK2412714T5 (en) | 2017-02-27 |
| BRPI1013548A2 (pt) | 2016-09-20 |
| ES2562637T3 (es) | 2016-03-07 |
| WO2010109386A1 (es) | 2010-09-30 |
| KR20110130420A (ko) | 2011-12-05 |
| BRPI1013548B1 (pt) | 2020-06-09 |
| CA2754154A1 (en) | 2010-09-30 |
| EP2412714B1 (en) | 2015-11-11 |
| AR075931A1 (es) | 2011-05-04 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant, registration |