NO330800B1 - Polynukleotid som koder for nye streptokokkantigener, vertscelle og vektor inneholdende polynukleotidet, fremgangsmate for fremstilling, vaksine sammensetning og anvendelse av denne - Google Patents
Polynukleotid som koder for nye streptokokkantigener, vertscelle og vektor inneholdende polynukleotidet, fremgangsmate for fremstilling, vaksine sammensetning og anvendelse av denne Download PDFInfo
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/16—Otologicals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Public Health (AREA)
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- Molecular Biology (AREA)
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- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Neurology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
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|---|---|---|---|
| US11380098P | 1998-12-23 | 1998-12-23 | |
| PCT/CA1999/001218 WO2000039299A2 (fr) | 1998-12-23 | 1999-12-20 | Antigenes de streptococcus |
Publications (3)
| Publication Number | Publication Date |
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| NO20013045D0 NO20013045D0 (no) | 2001-06-19 |
| NO20013045L NO20013045L (no) | 2001-08-20 |
| NO330800B1 true NO330800B1 (no) | 2011-07-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| NO20013045A NO330800B1 (no) | 1998-12-23 | 2001-06-19 | Polynukleotid som koder for nye streptokokkantigener, vertscelle og vektor inneholdende polynukleotidet, fremgangsmate for fremstilling, vaksine sammensetning og anvendelse av denne |
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| JP (2) | JP4761623B2 (fr) |
| KR (4) | KR100802198B1 (fr) |
| CN (3) | CN1191362C (fr) |
| AP (1) | AP2001002199A0 (fr) |
| AR (1) | AR029322A1 (fr) |
| AT (1) | ATE394489T1 (fr) |
| AU (1) | AU1764900A (fr) |
| BR (1) | BR9916477A (fr) |
| CA (1) | CA2356836C (fr) |
| CL (1) | CL2009002037A1 (fr) |
| CY (3) | CY1108223T1 (fr) |
| CZ (2) | CZ302790B6 (fr) |
| DE (1) | DE69938670D1 (fr) |
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| EA (1) | EA007409B1 (fr) |
| ES (3) | ES2306528T3 (fr) |
| HK (1) | HK1118575A1 (fr) |
| HU (1) | HU229664B1 (fr) |
| IL (3) | IL143905A0 (fr) |
| MX (1) | MXPA01006427A (fr) |
| NO (1) | NO330800B1 (fr) |
| NZ (1) | NZ512574A (fr) |
| OA (1) | OA11736A (fr) |
| PL (3) | PL205041B1 (fr) |
| PT (3) | PT1141306E (fr) |
| TR (2) | TR200200633T2 (fr) |
| UY (1) | UY25877A1 (fr) |
| WO (1) | WO2000039299A2 (fr) |
| ZA (1) | ZA200105114B (fr) |
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| ES2540281T3 (es) | 1998-02-20 | 2015-07-09 | Id Biomedical Corporation Of Quebec | Antígenos de estreptococos del grupo B |
| US20030134407A1 (en) | 1998-07-27 | 2003-07-17 | Le Page Richard William Falla | Nucleic acids and proteins from Streptococcus pneumoniae |
| EP1801218A3 (fr) * | 1998-07-27 | 2007-10-10 | Sanofi Pasteur Limited | Acides nucléiques et protéines de la pneumonie à streptocoques |
| EP1140157B1 (fr) | 1998-12-21 | 2009-02-18 | MedImmune, Inc. | Proteines de streptococcus pneumoniae et fragments immunogenes pour vaccins |
| TR200200633T2 (tr) * | 1998-12-23 | 2002-06-21 | Shire Biochem Inc. | Yeni streptococcus antijenleri |
| US7128918B1 (en) | 1998-12-23 | 2006-10-31 | Id Biomedical Corporation | Streptococcus antigens |
| US6833134B2 (en) | 2000-06-12 | 2004-12-21 | University Of Saskacthewan | Immunization of dairy cattle with GapC protein against Streptococcus infection |
| EP1294771B1 (fr) | 2000-06-12 | 2008-10-29 | University Of Saskatchewan | Protéine chimérique GapC de Streptococcus et son utilisation pour la vaccination et la diagnostique |
| US6866855B2 (en) | 2000-06-12 | 2005-03-15 | University Of Saskatchewan | Immunization of dairy cattle with GapC protein against Streptococcus infection |
| NZ553554A (en) | 2000-06-20 | 2008-11-28 | Id Biomedical Corp | Streptococcus antigens |
| US7074415B2 (en) | 2000-06-20 | 2006-07-11 | Id Biomedical Corporation | Streptococcus antigens |
| AU2002242549A1 (en) * | 2001-03-30 | 2002-10-15 | Shire Biochem Inc. | Streptococcus pyogenes antigens and corresponding dna fragments |
| DE60234772D1 (de) | 2001-07-06 | 2010-01-28 | Id Biomedical Corp | Gruppe-b streptococcus antigene und entsprechende dna-fragmente |
| GB0130228D0 (en) * | 2001-12-18 | 2002-02-06 | Hansa Medica Ab | Protein |
| US7262024B2 (en) | 2001-12-20 | 2007-08-28 | Id Biomedical Corporation | Streptococcus antigens |
| WO2004078907A2 (fr) | 2003-03-04 | 2004-09-16 | Intercell Ag | Antigenes vis-a-vis du streptococcus pyogenes |
| EP2333114A1 (fr) * | 2003-04-15 | 2011-06-15 | Intercell AG | S. pneumoniae antigènes |
| LT3017827T (lt) | 2005-12-22 | 2019-01-10 | Glaxosmithkline Biologicals S.A. | Pneumokokinė polisacharidinė konjuguota vakcina |
| GB0607088D0 (en) | 2006-04-07 | 2006-05-17 | Glaxosmithkline Biolog Sa | Vaccine |
| KR20100045445A (ko) | 2007-06-26 | 2010-05-03 | 글락소스미스클라인 바이오로지칼즈 에스.에이. | 스트렙토코쿠스 뉴모니애 캡슐 다당류 컨쥬게이트를 포함하는 백신 |
| CA2694466A1 (fr) * | 2007-07-23 | 2009-01-29 | Sanofi Pasteur Limited | Polypeptides immunogenes et anticorps monoclonaux |
| JP5749642B2 (ja) * | 2008-04-16 | 2015-07-15 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | ワクチン |
| AU2009296458A1 (en) | 2008-09-26 | 2010-04-01 | Nanobio Corporation | Nanoemulsion therapeutic compositions and methods of using the same |
| BRPI1014494A2 (pt) | 2009-04-30 | 2016-08-02 | Coley Pharm Group Inc | vacina pneumocócica e usos da mesma |
| WO2010132833A1 (fr) | 2009-05-14 | 2010-11-18 | The Regents Of The University Of Michigan | Compositions vaccinales contre streptococcus et méthodes d'utilisation associées |
| PE20161560A1 (es) | 2009-09-03 | 2017-01-11 | Pfizer Vaccines Llc | Vacuna de pcsk9 |
| GB201003920D0 (en) | 2010-03-09 | 2010-04-21 | Glaxosmithkline Biolog Sa | Method of treatment |
| BR112013013702A2 (pt) | 2010-12-03 | 2016-09-13 | Sanofi Pasteur Ltd | composição para imunização contra streptococcus pneumoniae |
| AU2012207089B2 (en) | 2011-01-20 | 2016-04-14 | Children's Medical Center Corporation | Vaccines and compositions against Streptococcus pneumoniae |
| US20140004142A1 (en) | 2011-03-02 | 2014-01-02 | Pfizer Inc. | Pcsk9 vaccine |
| BR112013029514A2 (pt) | 2011-05-17 | 2019-09-24 | Glaxosmithkline Biologicals Sa | composição imunogênica, vacina, e, método de tratar ou impedir uma doença |
| BR112014016612A2 (pt) * | 2012-01-05 | 2017-06-27 | Deutsches Krebsforsch | meios e métodos para tratar ou diagnosticar cânceres positivos para a mutação idh1 r132h |
| EP2931743A4 (fr) * | 2012-12-14 | 2016-08-03 | Sanofi Pasteur Ltd | Méthodes pour évaluer l'immunogénicité |
| ES2883343T3 (es) | 2014-01-21 | 2021-12-07 | Pfizer | Polisacáridos capsulares de Streptococcus pneumoniae y conjugados de los mismos |
| US11160855B2 (en) | 2014-01-21 | 2021-11-02 | Pfizer Inc. | Immunogenic compositions comprising conjugated capsular saccharide antigens and uses thereof |
| EP3096785B1 (fr) | 2014-01-21 | 2020-09-09 | Pfizer Inc | Compositions immunogènes comprenant des antigènes saccharidiques capsulaires conjugués et leurs utilisations |
| EP3583947B1 (fr) | 2014-01-21 | 2023-10-11 | Pfizer Inc. | Polysaccharides capsulaires de streptococcus pneumoniae et leurs conjugués |
| US10668164B2 (en) | 2014-02-14 | 2020-06-02 | Pfizer Inc. | Immunogenic glycoprotein conjugates |
| DK3244917T5 (da) | 2015-01-15 | 2024-10-14 | Pfizer Inc | Immunogene sammensætninger til anvendelse i pneumokokvacciner |
| PE20180657A1 (es) | 2015-07-21 | 2018-04-17 | Pfizer | Composiciones inmunogenas que comprenden antigenos de sacarido capsular conjugados, kits que las comprenden y sus usos |
| GB201518684D0 (en) | 2015-10-21 | 2015-12-02 | Glaxosmithkline Biolog Sa | Vaccine |
| CA3005524C (fr) | 2015-11-20 | 2023-10-10 | Pfizer Inc. | Compositions immunogenes destinees a etre utilisees dans des vaccins pneumococciques |
| GB201610599D0 (en) | 2016-06-17 | 2016-08-03 | Glaxosmithkline Biologicals Sa | Immunogenic Composition |
| SI3570879T1 (sl) | 2017-01-20 | 2022-06-30 | Pfizer Inc. | Imunogenska kompozicija za uporabo v pnevmokoknih cepivih |
| US11260119B2 (en) | 2018-08-24 | 2022-03-01 | Pfizer Inc. | Escherichia coli compositions and methods thereof |
| JP2022512345A (ja) | 2018-12-12 | 2022-02-03 | ファイザー・インク | 免疫原性多重ヘテロ抗原多糖－タンパク質コンジュゲートおよびその使用 |
| JP7239509B6 (ja) | 2019-02-22 | 2023-03-28 | ファイザー・インク | 細菌多糖類を精製するための方法 |
| US20220184199A1 (en) | 2019-04-10 | 2022-06-16 | Pfizer Inc. | Immunogenic compositions comprising conjugated capsular saccharide antigens, kits comprising the same and uses thereof |
| MX2022001241A (es) | 2019-07-31 | 2022-04-20 | Sanofi Pasteur Inc | Composiciones de conjugados neumococicos multivalentes polisacarido - proteina y metodos para usar los mismos. |
| IL292494A (en) | 2019-11-01 | 2022-06-01 | Pfizer | Preparations of Escherichia coli and their methods |
| WO2021165847A1 (fr) | 2020-02-21 | 2021-08-26 | Pfizer Inc. | Purification de saccharides |
| EP4107170A2 (fr) | 2020-02-23 | 2022-12-28 | Pfizer Inc. | Escherichia coli compositions d'et méthodes associées |
| JP2023546446A (ja) | 2020-10-22 | 2023-11-02 | ファイザー・インク | 細菌多糖を精製する方法 |
| IL302362A (en) | 2020-10-27 | 2023-06-01 | Pfizer | ESCHERICHIA COLI preparations and their methods |
| CN116744965A (zh) | 2020-11-04 | 2023-09-12 | 辉瑞大药厂 | 用于肺炎球菌疫苗的免疫原性组合物 |
| US20220202923A1 (en) | 2020-12-23 | 2022-06-30 | Pfizer Inc. | E. coli fimh mutants and uses thereof |
| CA3218544A1 (fr) | 2021-05-03 | 2022-11-10 | Pfizer Inc. | Vaccination contre des infections bacteriennes et a betacoronavirus |
| WO2022234416A1 (fr) | 2021-05-03 | 2022-11-10 | Pfizer Inc. | Vaccination contre des infections à pneumocoque et à covid-19 |
| US20220387576A1 (en) | 2021-05-28 | 2022-12-08 | Pfizer Inc. | Immunogenic compositions comprising conjugated capsular saccharide antigens and uses thereof |
| JP2024522395A (ja) | 2021-05-28 | 2024-06-19 | ファイザー・インク | コンジュゲートさせた莢膜糖抗原を含む免疫原性組成物およびその使用 |
| AU2023207315A1 (en) | 2022-01-13 | 2024-06-27 | Pfizer Inc. | Immunogenic compositions comprising conjugated capsular saccharide antigens and uses thereof |
| WO2023161817A1 (fr) | 2022-02-25 | 2023-08-31 | Pfizer Inc. | Procédés d'incorporation de groupes azido dans des polysaccharides capsulaires bactériens |
| WO2023218322A1 (fr) | 2022-05-11 | 2023-11-16 | Pfizer Inc. | Procédé de production de formulations de vaccin avec des conservateurs |
| WO2024084397A1 (fr) | 2022-10-19 | 2024-04-25 | Pfizer Inc. | Vaccination contre infections à pneumocoques et à covid-19 |
| WO2024110827A1 (fr) | 2022-11-21 | 2024-05-30 | Pfizer Inc. | Procédés de préparation d'antigènes saccharidiques capsulaires conjugués et leurs utilisations |
| US20240181028A1 (en) | 2022-11-22 | 2024-06-06 | Pfizer Inc. | Immunogenic compositions comprising conjugated capsular saccharide antigens and uses thereof |
| WO2024116096A1 (fr) | 2022-12-01 | 2024-06-06 | Pfizer Inc. | Formulations de vaccin pneumococcique conjugué |
| WO2024166008A1 (fr) | 2023-02-10 | 2024-08-15 | Pfizer Inc. | Compositions immunogènes comprenant des antigènes saccharidiques capsulaires conjugués et leurs utilisations |
| WO2024201324A2 (fr) | 2023-03-30 | 2024-10-03 | Pfizer Inc. | Compositions immunogènes comprenant des antigènes saccharidiques capsulaires conjugués et utilisations associées |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4431739A (en) | 1979-11-05 | 1984-02-14 | Genentech, Inc. | Transformant bacterial culture capable of expressing heterologous protein |
| US4425437A (en) | 1979-11-05 | 1984-01-10 | Genentech, Inc. | Microbial polypeptide expression vehicle |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| CN1046291C (zh) * | 1993-03-19 | 1999-11-10 | G·林达尔 | Rib蛋白、其纯化方法、检测试剂盒及药物组合物 |
| TR199701537T1 (xx) * | 1995-06-07 | 1998-03-21 | Biochem Vaccines Inc. | HSP70 ailesinin streptokokal �s� �ok protein �yeleri. |
| US5928895A (en) * | 1996-09-24 | 1999-07-27 | Smithkline Beecham Corporation | IgA Fc binding protein |
| US5882896A (en) * | 1996-09-24 | 1999-03-16 | Smithkline Beecham Corporation | M protein |
| US6420135B1 (en) * | 1996-10-31 | 2002-07-16 | Human Genome Sciences, Inc. | Streptococcus pneumoniae polynucleotides and sequences |
| KR20010024299A (ko) * | 1997-09-24 | 2001-03-26 | 리전츠 오브 더 유니버스티 오브 미네소타 | 스트렙토코쿠스 뉴모니애로부터의 사람 보체 ｃ3 분해단백질분해효소 |
| EP1073450A4 (fr) * | 1998-04-23 | 2003-04-23 | Uab Research Foundation | Proteine de surface pneumococcique c(pspc), regions epitopes et selection de souches d'une telle proteine, et utilisations correspondantes |
| CN1318103A (zh) * | 1998-07-27 | 2001-10-17 | 微生物技术有限公司 | 肺炎链球菌的核酸和蛋白质 |
| JP2002531055A (ja) * | 1998-07-27 | 2002-09-24 | マイクロビアル テクニクス リミティッド | 肺炎連鎖球菌のタンパク質及び核酸分子 |
| MXPA01003073A (es) * | 1998-09-24 | 2002-04-24 | Univ Minnesota | Polipeptido que degrada la proteina c3 complementaria humana a partir de streptococcus pneumoniae. |
| EP1140157B1 (fr) * | 1998-12-21 | 2009-02-18 | MedImmune, Inc. | Proteines de streptococcus pneumoniae et fragments immunogenes pour vaccins |
| TR200200633T2 (tr) * | 1998-12-23 | 2002-06-21 | Shire Biochem Inc. | Yeni streptococcus antijenleri |
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1999
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2001
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2008
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2009
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