NO322135B1 - Termostabilt DNA polymerase-enzym, nukleinsyresekvens som koder for enzymet, vektor og vertscelle som omfatter nukleinsyresekvensen, fremgangsmate for fremstilling av, og anvendelse av termostabilt DNA-polymerase-enzym, samt blanding, fremgangsmate og testsett hvor enzymet inngar. - Google Patents
Termostabilt DNA polymerase-enzym, nukleinsyresekvens som koder for enzymet, vektor og vertscelle som omfatter nukleinsyresekvensen, fremgangsmate for fremstilling av, og anvendelse av termostabilt DNA-polymerase-enzym, samt blanding, fremgangsmate og testsett hvor enzymet inngar. Download PDFInfo
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- NO322135B1 NO322135B1 NO19973595A NO973595A NO322135B1 NO 322135 B1 NO322135 B1 NO 322135B1 NO 19973595 A NO19973595 A NO 19973595A NO 973595 A NO973595 A NO 973595A NO 322135 B1 NO322135 B1 NO 322135B1
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- Prior art keywords
- dna polymerase
- thermostable dna
- enzyme
- sequencing
- sequence
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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WO1998040496A1 (en) | 1997-03-12 | 1998-09-17 | The Perkin-Elmer Corporation | Dna polymerases having improved labeled nucleotide incorporation properties |
US6346379B1 (en) * | 1997-09-11 | 2002-02-12 | F. Hoffman-La Roche Ag | Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes |
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US6610492B1 (en) | 1998-10-01 | 2003-08-26 | Variagenics, Inc. | Base-modified nucleotides and cleavage of polynucleotides incorporating them |
US6440705B1 (en) | 1998-10-01 | 2002-08-27 | Vincent P. Stanton, Jr. | Method for analyzing polynucleotides |
AU5008900A (en) * | 1999-05-12 | 2000-11-21 | Invitrogen Corporation | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
GB9921318D0 (en) * | 1999-09-09 | 1999-11-10 | Kristensen Tom | Chimeric molecules |
US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
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US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
US20020102577A1 (en) * | 2000-07-31 | 2002-08-01 | Raillard Sun Ai | Nucleotide incorporating enzymes |
US6811979B2 (en) * | 2000-10-11 | 2004-11-02 | Applera Corporation | Fluorescent nucleobase conjugates having anionic linkers |
IL155450A0 (en) * | 2000-10-20 | 2003-11-23 | Expression Diagnostics Inc | Leukocyte expression profiling |
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