MX9705961A - Polimerasa de adn termoestable, modificada. - Google Patents

Polimerasa de adn termoestable, modificada.

Info

Publication number
MX9705961A
MX9705961A MX9705961A MX9705961A MX9705961A MX 9705961 A MX9705961 A MX 9705961A MX 9705961 A MX9705961 A MX 9705961A MX 9705961 A MX9705961 A MX 9705961A MX 9705961 A MX9705961 A MX 9705961A
Authority
MX
Mexico
Prior art keywords
ntps
ribo
thermostable dna
dna polymerase
dna
Prior art date
Application number
MX9705961A
Other languages
English (en)
Other versions
MXPA97005961A (es
Inventor
David Harrow Gelfand
Lisa Vivian Kalman
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MXPA97005961A publication Critical patent/MXPA97005961A/es
Publication of MX9705961A publication Critical patent/MX9705961A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Abstract

La invencion proporciona enzimas ADN polimerasa que comprenden la secuencia aminoácido SerGlnIleXaaLeuArgXaa (SEQ ID NO: 1), en donde "Xaa" en posicion 4 de esta secuencia es cualquier residuo aminoácido pero no un residuo ácido glutámico (Glu), preferiblemente un residuo glicina y "Xaa" en posicion 7 de esta secuencia es un residuo valina (Val) o un residuo isoleucina (Ile). Las ADN polimerasas termoestables de la invencion tienen la eficiencia aumentada para incorporar nucleotidos no convencionales, tales como ribonucléotidos, en productos de ADN y son ventajosos en muchas aplicaciones de síntesis in vitro. Tales enzimas son particularmente utiles para usar en protocolos de secuenciacion de ácido nucleico y proporcionar nuevos medios para el análisis de la secuencia de ADN con ventajas de costo y eficiencia. También se reivindican ácidos nucleicos que codifican dichas polimerasas, vectores y células huésped que comprenden tal ácido nucleico, así como las composiciones para usar en una reaccion de secuenciacion, los equipos y métodos para la secuenciacion que incluyen tales polimerasas.
MX9705961A 1996-08-06 1997-08-05 Polimerasa de adn termoestable, modificada. MX9705961A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2337696P 1996-08-06 1996-08-06
US023376 1996-08-06

Publications (2)

Publication Number Publication Date
MXPA97005961A MXPA97005961A (es) 1998-02-01
MX9705961A true MX9705961A (es) 1998-02-28

Family

ID=21814728

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9705961A MX9705961A (es) 1996-08-06 1997-08-05 Polimerasa de adn termoestable, modificada.

Country Status (21)

Country Link
US (1) US5939292A (es)
EP (1) EP0823479B1 (es)
JP (1) JP3170229B2 (es)
KR (1) KR100510619B1 (es)
CN (1) CN1154733C (es)
AT (1) ATE278020T1 (es)
AU (1) AU714929B2 (es)
BR (1) BR9704260B1 (es)
CA (1) CA2210951C (es)
CZ (1) CZ293215B6 (es)
DE (1) DE69730926T2 (es)
DK (1) DK0823479T3 (es)
ES (1) ES2227640T3 (es)
HU (1) HUP9701341A3 (es)
IL (1) IL121441A (es)
MX (1) MX9705961A (es)
NO (1) NO322135B1 (es)
PL (1) PL321478A1 (es)
RU (1) RU2235773C2 (es)
TW (1) TW528802B (es)
UA (1) UA47423C2 (es)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
AU743025B2 (en) 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
US6346379B1 (en) * 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
AU5008900A (en) * 1999-05-12 2000-11-21 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
US20020127623A1 (en) * 2000-07-31 2002-09-12 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
EP1317464B1 (en) * 2000-10-11 2009-05-13 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
NZ525336A (en) * 2000-10-20 2006-03-31 Expression Diagnostics Inc Leukocyte expression profiling
WO2002073504A1 (en) * 2001-03-14 2002-09-19 Gene Logic, Inc. A system and method for retrieving and using gene expression data from multiple sources
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
EP1436385A4 (en) * 2001-09-14 2005-12-14 Invitrogen Corp DNA POLYMERASES AND MUTANTS CORRESPONDING
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7890268B2 (en) * 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
US7645866B2 (en) * 2005-06-28 2010-01-12 Life Technologies Corporation Methods of producing and sequencing modified polynucleotides
EP1762629B1 (en) 2005-09-12 2009-11-11 Roche Diagnostics GmbH Detection of biological DNA
US20070154894A1 (en) * 2005-12-30 2007-07-05 Affymetrix, Inc. Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2011123246A2 (en) 2010-04-01 2011-10-06 Illumina, Inc. Solid-phase clonal amplification and related methods
JPWO2012070618A1 (ja) 2010-11-24 2014-05-19 株式会社カネカ 増幅核酸検出方法及び検出デバイス
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
CN104254620B (zh) * 2012-04-27 2022-11-08 株式会社钟化 核酸的扩增方法及扩增核酸的检测方法
CN103898077B (zh) 2012-12-24 2017-01-11 财团法人工业技术研究院 单离的脱氧核糖核酸聚合酶、套组及其应用
ES2713653T3 (es) 2013-03-15 2019-05-23 Ibis Biosciences Inc Análogos nucleotídicos para secuenciación
US9932623B2 (en) 2013-08-19 2018-04-03 Abbott Molecular Inc. Nucleotide analogs
WO2015076356A1 (ja) 2013-11-22 2015-05-28 株式会社カネカ 短鎖ｒｎａの検出方法
CN103966182A (zh) * 2014-04-30 2014-08-06 厦门安普利生物工程有限公司 一种提取纯化Tth DNA聚合酶的方法
KR102154812B1 (ko) * 2014-09-29 2020-09-11 박진우 광고 및 태양광 충전 기능을 가진 가방
EP3091026B1 (en) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2019014359A2 (en) * 2017-07-12 2019-01-17 The Scripps Research Institute POLYMERASE CHAIN TRANSCRIPTION (PCT): EXPONENTIAL SYNTHESIS OF RNA AND MODIFIED RNA
EP3684955A1 (en) 2017-09-20 2020-07-29 Regeneron Pharmaceuticals, Inc. Immunotherapy methods for patients whose tumors carry a high passenger gene mutation burden
CN108130318B (zh) * 2018-02-28 2020-07-14 深圳市艾伟迪生物科技有限公司 突变型Taq DNA聚合酶、免核酸提取直接PCR扩增的试剂盒及其应用
EP3856753A4 (en) 2018-09-28 2022-11-16 Centrillion Technology Holdings Corporation DISULFIDE CROSSLINKED REVERSIBLE TERMINATORS
CN110093410A (zh) * 2019-05-21 2019-08-06 通用生物系统（安徽）有限公司 一种dna测序反应试剂及其制备方法
GB202007428D0 (en) 2020-05-19 2020-07-01 Fabricnano Ltd Polynucleotide synthesis
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
GB202114105D0 (en) 2021-10-01 2021-11-17 Fabricnano Ltd Nucleotide synthesis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0655506B1 (en) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNA polymerases having modified nucleotide binding site for DNA sequencing

Also Published As

Publication number Publication date
JPH1066588A (ja) 1998-03-10
HU9701341D0 (en) 1997-09-29
HUP9701341A3 (en) 2000-12-28
RU2235773C2 (ru) 2004-09-10
CZ229997A3 (cs) 1998-06-17
PL321478A1 (en) 1998-02-16
DK0823479T3 (da) 2005-01-31
NO973595D0 (no) 1997-08-05
ES2227640T3 (es) 2005-04-01
DE69730926T2 (de) 2006-02-23
CN1177640A (zh) 1998-04-01
DE69730926D1 (de) 2004-11-04
AU714929B2 (en) 2000-01-13
CA2210951C (en) 2009-02-10
ATE278020T1 (de) 2004-10-15
IL121441A (en) 2000-07-16
TW528802B (en) 2003-04-21
BR9704260A (pt) 1998-09-15
UA47423C2 (uk) 2002-07-15
CN1154733C (zh) 2004-06-23
EP0823479B1 (en) 2004-09-29
HUP9701341A2 (hu) 1999-06-28
IL121441A0 (en) 1998-01-04
CZ293215B6 (cs) 2004-03-17
EP0823479A2 (en) 1998-02-11
NO322135B1 (no) 2006-08-21
US5939292A (en) 1999-08-17
NO973595L (no) 1998-02-09
EP0823479A3 (en) 2000-03-29
BR9704260B1 (pt) 2010-09-08
AU3319797A (en) 1998-02-12
JP3170229B2 (ja) 2001-05-28
KR100510619B1 (ko) 2005-12-21
KR19980018395A (ko) 1998-06-05
CA2210951A1 (en) 1998-02-06

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