NO973595L - Modifisert termostabil DNA polymerase - Google Patents

Modifisert termostabil DNA polymerase

Info

Publication number
NO973595L
NO973595L NO973595A NO973595A NO973595L NO 973595 L NO973595 L NO 973595L NO 973595 A NO973595 A NO 973595A NO 973595 A NO973595 A NO 973595A NO 973595 L NO973595 L NO 973595L
Authority
NO
Norway
Prior art keywords
residue
sequence
dna
polymerases
thermostable dna
Prior art date
Application number
NO973595A
Other languages
English (en)
Other versions
NO973595D0 (no
NO322135B1 (no
Inventor
David Harrow Gelfand
Lisa Vivian Kalman
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of NO973595D0 publication Critical patent/NO973595D0/no
Publication of NO973595L publication Critical patent/NO973595L/no
Publication of NO322135B1 publication Critical patent/NO322135B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
NO19973595A 1996-08-06 1997-08-05 Termostabilt DNA polymerase-enzym, nukleinsyresekvens som koder for enzymet, vektor og vertscelle som omfatter nukleinsyresekvensen, fremgangsmate for fremstilling av, og anvendelse av termostabilt DNA-polymerase-enzym, samt blanding, fremgangsmate og testsett hvor enzymet inngar. NO322135B1 (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2337696P 1996-08-06 1996-08-06

Publications (3)

Publication Number Publication Date
NO973595D0 NO973595D0 (no) 1997-08-05
NO973595L true NO973595L (no) 1998-02-09
NO322135B1 NO322135B1 (no) 2006-08-21

Family

ID=21814728

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19973595A NO322135B1 (no) 1996-08-06 1997-08-05 Termostabilt DNA polymerase-enzym, nukleinsyresekvens som koder for enzymet, vektor og vertscelle som omfatter nukleinsyresekvensen, fremgangsmate for fremstilling av, og anvendelse av termostabilt DNA-polymerase-enzym, samt blanding, fremgangsmate og testsett hvor enzymet inngar.

Country Status (21)

Country Link
US (1) US5939292A (no)
EP (1) EP0823479B1 (no)
JP (1) JP3170229B2 (no)
KR (1) KR100510619B1 (no)
CN (1) CN1154733C (no)
AT (1) ATE278020T1 (no)
AU (1) AU714929B2 (no)
BR (1) BR9704260B1 (no)
CA (1) CA2210951C (no)
CZ (1) CZ293215B6 (no)
DE (1) DE69730926T2 (no)
DK (1) DK0823479T3 (no)
ES (1) ES2227640T3 (no)
HU (1) HUP9701341A3 (no)
IL (1) IL121441A (no)
MX (1) MX9705961A (no)
NO (1) NO322135B1 (no)
PL (1) PL321478A1 (no)
RU (1) RU2235773C2 (no)
TW (1) TW528802B (no)
UA (1) UA47423C2 (no)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
EP0983364B1 (en) * 1997-03-12 2002-06-12 PE Corporation (NY) Dna polymerases having improved labeled nucleotide incorporation properties
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
WO2002010750A2 (en) * 2000-07-31 2002-02-07 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
ATE431355T1 (de) * 2000-10-11 2009-05-15 Applera Corp Fluoreszierende nukleobasekonjugate mit anionische linker
ZA200303132B (en) * 2000-10-20 2004-09-23 Expression Diagnostics Inc Leukocyte expression profiling.
WO2002073504A1 (en) * 2001-03-14 2002-09-19 Gene Logic, Inc. A system and method for retrieving and using gene expression data from multiple sources
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
WO2006009870A2 (en) * 2004-06-17 2006-01-26 Epigenomics Ag Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7890268B2 (en) * 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
JP2008546424A (ja) * 2005-06-28 2008-12-25 アジェンコート パーソナル ジェノミクス コーポレイション 修飾ポリヌクレオチドを作製する方法および配列決定する方法
EP1762629B1 (en) 2005-09-12 2009-11-11 Roche Diagnostics GmbH Detection of biological DNA
US20070154894A1 (en) * 2005-12-30 2007-07-05 Affymetrix, Inc. Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2011123246A2 (en) 2010-04-01 2011-10-06 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2012070618A1 (ja) 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
WO2013162026A1 (ja) * 2012-04-27 2013-10-31 株式会社カネカ 核酸の増幅方法、および、増幅核酸の検出方法
CN103898077B (zh) 2012-12-24 2017-01-11 财团法人工业技术研究院 单离的脱氧核糖核酸聚合酶、套组及其应用
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
ES2805004T3 (es) 2013-08-19 2021-02-10 Abbott Molecular Inc Análogos de nucleótidos
JP6691380B2 (ja) 2013-11-22 2020-04-28 株式会社カネカ 短鎖ｒｎａの検出方法
CN103966182A (zh) * 2014-04-30 2014-08-06 厦门安普利生物工程有限公司 一种提取纯化Tth DNA聚合酶的方法
KR102154812B1 (ko) * 2014-09-29 2020-09-11 박진우 광고 및 태양광 충전 기능을 가진 가방
EP3091026B1 (en) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2019014359A2 (en) * 2017-07-12 2019-01-17 The Scripps Research Institute POLYMERASE CHAIN TRANSCRIPTION (PCT): EXPONENTIAL SYNTHESIS OF RNA AND MODIFIED RNA
JP2020536051A (ja) 2017-09-20 2020-12-10 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 腫瘍が高いパッセンジャー遺伝子変異量を担持する患者の免疫療法剤
CN108130318B (zh) * 2018-02-28 2020-07-14 深圳市艾伟迪生物科技有限公司 突变型Taq DNA聚合酶、免核酸提取直接PCR扩增的试剂盒及其应用
US20220048940A1 (en) 2018-09-28 2022-02-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
MX2021004925A (es) 2018-10-29 2021-06-15 Codexis Inc Variantes de adn-polimerasa modificadas.
CN110093410A (zh) * 2019-05-21 2019-08-06 通用生物系统（安徽）有限公司 一种dna测序反应试剂及其制备方法
GB202007428D0 (en) 2020-05-19 2020-07-01 Fabricnano Ltd Polynucleotide synthesis
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
GB202114105D0 (en) 2021-10-01 2021-11-17 Fabricnano Ltd Nucleotide synthesis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
DE69400567T2 (de) * 1994-10-17 1997-02-06 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing

Also Published As

Publication number Publication date
JPH1066588A (ja) 1998-03-10
IL121441A (en) 2000-07-16
AU3319797A (en) 1998-02-12
CN1154733C (zh) 2004-06-23
EP0823479B1 (en) 2004-09-29
EP0823479A3 (en) 2000-03-29
IL121441A0 (en) 1998-01-04
MX9705961A (es) 1998-02-28
TW528802B (en) 2003-04-21
CN1177640A (zh) 1998-04-01
ES2227640T3 (es) 2005-04-01
ATE278020T1 (de) 2004-10-15
HUP9701341A3 (en) 2000-12-28
DE69730926D1 (de) 2004-11-04
BR9704260A (pt) 1998-09-15
RU2235773C2 (ru) 2004-09-10
CA2210951C (en) 2009-02-10
PL321478A1 (en) 1998-02-16
BR9704260B1 (pt) 2010-09-08
US5939292A (en) 1999-08-17
AU714929B2 (en) 2000-01-13
HU9701341D0 (en) 1997-09-29
NO973595D0 (no) 1997-08-05
CZ229997A3 (cs) 1998-06-17
KR100510619B1 (ko) 2005-12-21
HUP9701341A2 (hu) 1999-06-28
DE69730926T2 (de) 2006-02-23
CZ293215B6 (cs) 2004-03-17
NO322135B1 (no) 2006-08-21
CA2210951A1 (en) 1998-02-06
KR19980018395A (ko) 1998-06-05
JP3170229B2 (ja) 2001-05-28
EP0823479A2 (en) 1998-02-11
DK0823479T3 (da) 2005-01-31
UA47423C2 (uk) 2002-07-15

Similar Documents

Publication Publication Date Title
NO973595L (no) Modifisert termostabil DNA polymerase
AU691374B2 (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
IL126100A0 (en) Modified thermostable dna polymerases for sequencing
EP1034302A4 (en) DE NOVO OR "UNIVERSAL" SEQUENCING ARRAY
EP0731174A3 (en) Nucleic acid sequence amplification methods
EP0892058A3 (en) Mutant chimeric DNA polymerases
EP1591541A3 (en) Method of nucleic acid sequencing
WO1992006188A3 (en) Thermostable dna polymerase
TW360712B (en) Nucleic acid sequence amplification method, composition and kit
CA2020958A1 (en) Nucleic acid sequence amplification methods
WO1996015264A3 (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
WO2001073052A3 (en) Thermophilic polymerase iii holoenzyme
WO1996014405A3 (en) Biologically active fragments of thermus flavus dna polymerase
Kacian et al. Kits for nucleic acid sequence amplification methods
MX9706158A (es) Quimiocinas novedosas expresadas en pancreas.

Legal Events

Date Code Title Description
MM1K Lapsed by not paying the annual fees