MX358177B - Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos. - Google Patents
Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos.Info
- Publication number
- MX358177B MX358177B MX2016013852A MX2016013852A MX358177B MX 358177 B MX358177 B MX 358177B MX 2016013852 A MX2016013852 A MX 2016013852A MX 2016013852 A MX2016013852 A MX 2016013852A MX 358177 B MX358177 B MX 358177B
- Authority
- MX
- Mexico
- Prior art keywords
- fatty alcohols
- improved production
- enzymes
- car
- car enzymes
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 150000002191 fatty alcohols Chemical class 0.000 title abstract 2
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
- C12Y102/99006—Carboxylate reductase (1.2.99.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/02—Thioester hydrolases (3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261619309P | 2012-04-02 | 2012-04-02 | |
| PCT/US2013/035040 WO2013152052A2 (en) | 2012-04-02 | 2013-04-02 | Novel car enzymes and improved production of fatty alcohols |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX358177B true MX358177B (es) | 2018-08-08 |
Family
ID=49237582
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016013852A MX358177B (es) | 2012-04-02 | 2013-04-02 | Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos. |
| MX2014011903A MX343296B (es) | 2012-04-02 | 2013-04-02 | Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014011903A MX343296B (es) | 2012-04-02 | 2013-04-02 | Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos. |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US9340801B2 (cg-RX-API-DMAC7.html) |
| EP (6) | EP3385374B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6230594B2 (cg-RX-API-DMAC7.html) |
| KR (6) | KR101831331B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN104704113B (cg-RX-API-DMAC7.html) |
| AU (2) | AU2013243602B2 (cg-RX-API-DMAC7.html) |
| BR (2) | BR112014024674B1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2883064A1 (cg-RX-API-DMAC7.html) |
| CO (1) | CO7170148A2 (cg-RX-API-DMAC7.html) |
| ES (3) | ES2786633T3 (cg-RX-API-DMAC7.html) |
| MX (2) | MX358177B (cg-RX-API-DMAC7.html) |
| MY (1) | MY170889A (cg-RX-API-DMAC7.html) |
| SI (1) | SI3385374T1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013152052A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2796559B1 (en) * | 2008-10-28 | 2018-09-12 | REG Life Sciences, LLC | Microbial cell for producing fatty alcohols |
| MX379251B (es) * | 2012-04-02 | 2025-03-11 | Genomatica Inc | Producción mejorada de derivados de ácidos grasos. |
| KR101831331B1 (ko) * | 2012-04-02 | 2018-02-23 | 알이지 라이프 사이언시스, 엘엘씨 | Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 |
| WO2014026162A1 (en) | 2012-08-10 | 2014-02-13 | Opx Biotechnologies, Inc. | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US10047383B2 (en) | 2013-03-15 | 2018-08-14 | Cargill, Incorporated | Bioproduction of chemicals |
| BR112015023473B1 (pt) * | 2013-03-15 | 2023-04-11 | Cargill, Incorporated | Microrganismo geneticamente modificado e método de produzir um produto |
| CN106795483A (zh) | 2013-07-19 | 2017-05-31 | 嘉吉公司 | 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法 |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| BR112017001105A2 (pt) | 2014-07-18 | 2018-07-24 | REG Life Sciences, LLC | produção microbiana de dióis graxos |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| CN105985987B (zh) * | 2015-03-02 | 2020-01-31 | 苏州安捷生物科技有限公司 | 一种生物法制备脂肪醇的方法 |
| WO2017161112A1 (en) * | 2016-03-17 | 2017-09-21 | Invista Technologies S.A R.L. | Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto |
| JP2020506702A (ja) | 2017-02-02 | 2020-03-05 | カーギル インコーポレイテッド | Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞 |
| KR102677413B1 (ko) | 2017-04-03 | 2024-06-24 | 게노마티카 인코포레이티드 | 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체 |
| EP3844179A4 (en) | 2018-08-31 | 2022-08-31 | Genomatica, Inc. | XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE |
| US12221644B2 (en) | 2018-08-31 | 2025-02-11 | Genomatica, Inc. | Production of non-native monounsaturated fatty acids in bacteria |
| KR20230160398A (ko) | 2021-03-30 | 2023-11-23 | 아사히 가세이 가부시키가이샤 | 카르복실산 환원 활성을 갖는 재조합 폴리펩티드 |
| EP4493683A1 (en) | 2022-03-16 | 2025-01-22 | Genomatica, Inc. | Acyl-acp thioesterase variants and uses thereof |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5028539A (en) | 1988-08-31 | 1991-07-02 | The University Of Florida | Ethanol production using engineered mutant E. coli |
| US5482846A (en) | 1988-08-31 | 1996-01-09 | University Of Florida | Ethanol production in Gram-positive microbes |
| US5424202A (en) | 1988-08-31 | 1995-06-13 | The University Of Florida | Ethanol production by recombinant hosts |
| US5000000A (en) | 1988-08-31 | 1991-03-19 | University Of Florida | Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes |
| EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5602030A (en) | 1994-03-28 | 1997-02-11 | University Of Florida Research Foundation | Recombinant glucose uptake system |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US7056714B2 (en) * | 2003-03-11 | 2006-06-06 | University Of Iowa Research Foundation, Inc. | Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use |
| DE102004052115A1 (de) | 2004-10-26 | 2006-04-27 | Westfälische Wilhelms-Universität Münster | Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung |
| EP2194119A3 (en) | 2006-05-19 | 2010-09-22 | LS9, Inc. | Production of fatty acids and derivatives thereof |
| CA2678915C (en) | 2007-03-28 | 2019-04-09 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| CA2679085C (en) | 2007-05-22 | 2018-04-10 | Ls9, Inc. | Hydrocarbon-producing genes and methods of their use |
| TWI351779B (en) | 2007-12-03 | 2011-11-01 | Advance Smart Ind Ltd | Apparatus and method for correcting residual capac |
| WO2009085278A1 (en) | 2007-12-21 | 2009-07-09 | Ls9, Inc. | Methods and compositions for producing olefins |
| CA3038491A1 (en) | 2008-10-07 | 2010-04-15 | REG Life Sciences, LLC | Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase |
| EP2796559B1 (en) * | 2008-10-28 | 2018-09-12 | REG Life Sciences, LLC | Microbial cell for producing fatty alcohols |
| US9175234B2 (en) | 2008-12-23 | 2015-11-03 | REG Life Sciences, LLC | Methods and compositions related to thioesterase enzymes |
| WO2010127318A2 (en) | 2009-05-01 | 2010-11-04 | The Regents Of The University Of California | Product of fatty acid esters from biomass polymers |
| EP2432890B1 (en) * | 2009-05-22 | 2015-09-02 | Codexis, Inc. | Engineered biosynthesis of fatty alcohols |
| AU2010306785B2 (en) * | 2009-10-13 | 2016-01-14 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto |
| WO2011100667A1 (en) | 2010-02-14 | 2011-08-18 | Ls9, Inc. | Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols |
| US20110250663A1 (en) | 2010-04-08 | 2011-10-13 | Ls9, Inc. | Methods and compositions related to fatty alcohol biosynthetic enzymes |
| EP3702363A1 (en) * | 2011-04-01 | 2020-09-02 | Genomatica, Inc. | Fadr overexpressing bacterial cells for improved production of fatty acids and derivatives thereof |
| US9133487B2 (en) * | 2011-04-01 | 2015-09-15 | Genomatica, Inc. | Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto |
| US8617862B2 (en) * | 2011-06-22 | 2013-12-31 | Genomatica, Inc. | Microorganisms for producing propylene and methods related thereto |
| KR101831331B1 (ko) * | 2012-04-02 | 2018-02-23 | 알이지 라이프 사이언시스, 엘엘씨 | Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 |
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2013
- 2013-04-02 KR KR1020147030704A patent/KR101831331B1/ko active Active
- 2013-04-02 KR KR1020207009760A patent/KR102282677B1/ko not_active Expired - Fee Related
- 2013-04-02 EP EP18160746.6A patent/EP3385374B1/en active Active
- 2013-04-02 SI SI201331706T patent/SI3385374T1/sl unknown
- 2013-04-02 EP EP13766728.3A patent/EP2834350B1/en active Active
- 2013-04-02 EP EP19212944.3A patent/EP3674399B1/en active Active
- 2013-04-02 MY MYPI2014002838A patent/MY170889A/en unknown
- 2013-04-02 ES ES18160746T patent/ES2786633T3/es active Active
- 2013-04-02 AU AU2013243602A patent/AU2013243602B2/en not_active Ceased
- 2013-04-02 KR KR1020177026945A patent/KR20170113710A/ko not_active Ceased
- 2013-04-02 CA CA2883064A patent/CA2883064A1/en active Pending
- 2013-04-02 MX MX2016013852A patent/MX358177B/es unknown
- 2013-04-02 ES ES13766728.3T patent/ES2613553T3/es active Active
- 2013-04-02 EP EP22202380.6A patent/EP4186974A1/en active Pending
- 2013-04-02 EP EP21191034.4A patent/EP3985108A1/en not_active Withdrawn
- 2013-04-02 CN CN201380026546.8A patent/CN104704113B/zh active Active
- 2013-04-02 BR BR112014024674-2A patent/BR112014024674B1/pt not_active IP Right Cessation
- 2013-04-02 BR BR122019026987-4A patent/BR122019026987B1/pt active IP Right Grant
- 2013-04-02 JP JP2015504688A patent/JP6230594B2/ja active Active
- 2013-04-02 KR KR1020217023277A patent/KR20210095226A/ko not_active Ceased
- 2013-04-02 WO PCT/US2013/035040 patent/WO2013152052A2/en not_active Ceased
- 2013-04-02 KR KR1020197005741A patent/KR102114841B1/ko not_active Expired - Fee Related
- 2013-04-02 US US14/390,350 patent/US9340801B2/en active Active
- 2013-04-02 EP EP16198810.0A patent/EP3153579B1/en not_active Not-in-force
- 2013-04-02 ES ES16198810.0T patent/ES2666157T3/es active Active
- 2013-04-02 MX MX2014011903A patent/MX343296B/es active IP Right Grant
- 2013-04-02 KR KR1020197017717A patent/KR102185762B1/ko not_active Expired - Fee Related
-
2014
- 2014-10-29 CO CO14240039A patent/CO7170148A2/es unknown
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2016
- 2016-05-13 US US15/154,636 patent/US9758769B2/en active Active
- 2016-09-20 AU AU2016231497A patent/AU2016231497B2/en not_active Ceased
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2017
- 2017-09-05 US US15/695,205 patent/US10301603B2/en active Active
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2019
- 2019-05-16 US US16/414,550 patent/US11034937B2/en active Active
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