AU2013243602B2 - Car enzymes and improved production of fatty alcohols - Google Patents
Car enzymes and improved production of fatty alcohols Download PDFInfo
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- AU2013243602B2 AU2013243602B2 AU2013243602A AU2013243602A AU2013243602B2 AU 2013243602 B2 AU2013243602 B2 AU 2013243602B2 AU 2013243602 A AU2013243602 A AU 2013243602A AU 2013243602 A AU2013243602 A AU 2013243602A AU 2013243602 B2 AU2013243602 B2 AU 2013243602B2
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- C12Y—ENZYMES
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- C12Y—ENZYMES
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- C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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| CN105985987B (zh) * | 2015-03-02 | 2020-01-31 | 苏州安捷生物科技有限公司 | 一种生物法制备脂肪醇的方法 |
| WO2017161112A1 (en) * | 2016-03-17 | 2017-09-21 | Invista Technologies S.A R.L. | Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto |
| JP2020506702A (ja) | 2017-02-02 | 2020-03-05 | カーギル インコーポレイテッド | Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞 |
| KR102677413B1 (ko) | 2017-04-03 | 2024-06-24 | 게노마티카 인코포레이티드 | 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체 |
| EP3844179A4 (en) | 2018-08-31 | 2022-08-31 | Genomatica, Inc. | XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE |
| US12221644B2 (en) | 2018-08-31 | 2025-02-11 | Genomatica, Inc. | Production of non-native monounsaturated fatty acids in bacteria |
| KR20230160398A (ko) | 2021-03-30 | 2023-11-23 | 아사히 가세이 가부시키가이샤 | 카르복실산 환원 활성을 갖는 재조합 폴리펩티드 |
| EP4493683A1 (en) | 2022-03-16 | 2025-01-22 | Genomatica, Inc. | Acyl-acp thioesterase variants and uses thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100105963A1 (en) * | 2008-10-28 | 2010-04-29 | Ls9, Inc. | Methods and compositions for producing fatty alcohols |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5028539A (en) | 1988-08-31 | 1991-07-02 | The University Of Florida | Ethanol production using engineered mutant E. coli |
| US5482846A (en) | 1988-08-31 | 1996-01-09 | University Of Florida | Ethanol production in Gram-positive microbes |
| US5424202A (en) | 1988-08-31 | 1995-06-13 | The University Of Florida | Ethanol production by recombinant hosts |
| US5000000A (en) | 1988-08-31 | 1991-03-19 | University Of Florida | Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes |
| EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5602030A (en) | 1994-03-28 | 1997-02-11 | University Of Florida Research Foundation | Recombinant glucose uptake system |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US7056714B2 (en) * | 2003-03-11 | 2006-06-06 | University Of Iowa Research Foundation, Inc. | Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use |
| DE102004052115A1 (de) | 2004-10-26 | 2006-04-27 | Westfälische Wilhelms-Universität Münster | Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung |
| EP2194119A3 (en) | 2006-05-19 | 2010-09-22 | LS9, Inc. | Production of fatty acids and derivatives thereof |
| CA2678915C (en) | 2007-03-28 | 2019-04-09 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| CA2679085C (en) | 2007-05-22 | 2018-04-10 | Ls9, Inc. | Hydrocarbon-producing genes and methods of their use |
| TWI351779B (en) | 2007-12-03 | 2011-11-01 | Advance Smart Ind Ltd | Apparatus and method for correcting residual capac |
| WO2009085278A1 (en) | 2007-12-21 | 2009-07-09 | Ls9, Inc. | Methods and compositions for producing olefins |
| CA3038491A1 (en) | 2008-10-07 | 2010-04-15 | REG Life Sciences, LLC | Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase |
| US9175234B2 (en) | 2008-12-23 | 2015-11-03 | REG Life Sciences, LLC | Methods and compositions related to thioesterase enzymes |
| WO2010127318A2 (en) | 2009-05-01 | 2010-11-04 | The Regents Of The University Of California | Product of fatty acid esters from biomass polymers |
| EP2432890B1 (en) * | 2009-05-22 | 2015-09-02 | Codexis, Inc. | Engineered biosynthesis of fatty alcohols |
| AU2010306785B2 (en) * | 2009-10-13 | 2016-01-14 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto |
| WO2011100667A1 (en) | 2010-02-14 | 2011-08-18 | Ls9, Inc. | Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols |
| US20110250663A1 (en) | 2010-04-08 | 2011-10-13 | Ls9, Inc. | Methods and compositions related to fatty alcohol biosynthetic enzymes |
| EP3702363A1 (en) * | 2011-04-01 | 2020-09-02 | Genomatica, Inc. | Fadr overexpressing bacterial cells for improved production of fatty acids and derivatives thereof |
| US9133487B2 (en) * | 2011-04-01 | 2015-09-15 | Genomatica, Inc. | Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto |
| US8617862B2 (en) * | 2011-06-22 | 2013-12-31 | Genomatica, Inc. | Microorganisms for producing propylene and methods related thereto |
| KR101831331B1 (ko) * | 2012-04-02 | 2018-02-23 | 알이지 라이프 사이언시스, 엘엘씨 | Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 |
-
2013
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100105963A1 (en) * | 2008-10-28 | 2010-04-29 | Ls9, Inc. | Methods and compositions for producing fatty alcohols |
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