JP6230594B2 - Ｃａｒ酵素、および改良された脂肪アルコール産生方法 - Google Patents

Ｃａｒ酵素、および改良された脂肪アルコール産生方法 Download PDF

Info

Publication number
JP6230594B2
JP6230594B2 JP2015504688A JP2015504688A JP6230594B2 JP 6230594 B2 JP6230594 B2 JP 6230594B2 JP 2015504688 A JP2015504688 A JP 2015504688A JP 2015504688 A JP2015504688 A JP 2015504688A JP 6230594 B2 JP6230594 B2 JP 6230594B2
Authority
JP
Japan
Prior art keywords
polypeptide
mutant
fatty alcohol
host cell
fatty
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015504688A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015512645A (ja
JP2015512645A5 (cg-RX-API-DMAC7.html
Inventor
デレク エル． グリーンフィールド
デレク エル． グリーンフィールド
エリザベス ジェイ． クラーク
エリザベス ジェイ． クラーク
イーライ エス． グローバン
イーライ エス． グローバン
ヴィクランス アルラガッダ
ヴィクランス アルラガッダ
スンウォン リー
スンウォン リー
シュエチ リ
シュエチ リ
チーハオ フ
チーハオ フ
バオロン チュ
バオロン チュ
Original Assignee
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー, アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー filed Critical アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Publication of JP2015512645A publication Critical patent/JP2015512645A/ja
Publication of JP2015512645A5 publication Critical patent/JP2015512645A5/ja
Application granted granted Critical
Publication of JP6230594B2 publication Critical patent/JP6230594B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006—Carboxylate reductase (1.2.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2015504688A 2012-04-02 2013-04-02 Ｃａｒ酵素、および改良された脂肪アルコール産生方法 Active JP6230594B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Publications (3)

Publication Number Publication Date
JP2015512645A JP2015512645A (ja) 2015-04-30
JP2015512645A5 JP2015512645A5 (cg-RX-API-DMAC7.html) 2016-09-29
JP6230594B2 true JP6230594B2 (ja) 2017-11-15

Family

ID=49237582

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015504688A Active JP6230594B2 (ja) 2012-04-02 2013-04-02 Ｃａｒ酵素、および改良された脂肪アルコール産生方法

Country Status (14)

Country Link
US (4) US9340801B2 (cg-RX-API-DMAC7.html)
EP (6) EP3385374B1 (cg-RX-API-DMAC7.html)
JP (1) JP6230594B2 (cg-RX-API-DMAC7.html)
KR (6) KR101831331B1 (cg-RX-API-DMAC7.html)
CN (1) CN104704113B (cg-RX-API-DMAC7.html)
AU (2) AU2013243602B2 (cg-RX-API-DMAC7.html)
BR (2) BR112014024674B1 (cg-RX-API-DMAC7.html)
CA (1) CA2883064A1 (cg-RX-API-DMAC7.html)
CO (1) CO7170148A2 (cg-RX-API-DMAC7.html)
ES (3) ES2786633T3 (cg-RX-API-DMAC7.html)
MX (2) MX358177B (cg-RX-API-DMAC7.html)
MY (1) MY170889A (cg-RX-API-DMAC7.html)
SI (1) SI3385374T1 (cg-RX-API-DMAC7.html)
WO (1) WO2013152052A2 (cg-RX-API-DMAC7.html)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2796559B1 (en) * 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
MX379251B (es) * 2012-04-02 2025-03-11 Genomatica Inc Producción mejorada de derivados de ácidos grasos.
KR101831331B1 (ko) * 2012-04-02 2018-02-23 알이지 라이프 사이언시스, 엘엘씨 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
BR112015023473B1 (pt) * 2013-03-15 2023-04-11 Cargill, Incorporated Microrganismo geneticamente modificado e método de produzir um produto
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112017001105A2 (pt) 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
WO2017161112A1 (en) * 2016-03-17 2017-09-21 Invista Technologies S.A R.L. Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
KR20230160398A (ko) 2021-03-30 2023-11-23 아사히 가세이 가부시키가이샤 카르복실산 환원 활성을 갖는 재조합 폴리펩티드
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US7056714B2 (en) * 2003-03-11 2006-06-06 University Of Iowa Research Foundation, Inc. Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use
DE102004052115A1 (de) 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
EP2194119A3 (en) 2006-05-19 2010-09-22 LS9, Inc. Production of fatty acids and derivatives thereof
CA2678915C (en) 2007-03-28 2019-04-09 Ls9, Inc. Enhanced production of fatty acid derivatives
CA2679085C (en) 2007-05-22 2018-04-10 Ls9, Inc. Hydrocarbon-producing genes and methods of their use
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP2796559B1 (en) * 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
US9175234B2 (en) 2008-12-23 2015-11-03 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
EP2432890B1 (en) * 2009-05-22 2015-09-02 Codexis, Inc. Engineered biosynthesis of fatty alcohols
AU2010306785B2 (en) * 2009-10-13 2016-01-14 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20110250663A1 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
EP3702363A1 (en) * 2011-04-01 2020-09-02 Genomatica, Inc. Fadr overexpressing bacterial cells for improved production of fatty acids and derivatives thereof
US9133487B2 (en) * 2011-04-01 2015-09-15 Genomatica, Inc. Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto
US8617862B2 (en) * 2011-06-22 2013-12-31 Genomatica, Inc. Microorganisms for producing propylene and methods related thereto
KR101831331B1 (ko) * 2012-04-02 2018-02-23 알이지 라이프 사이언시스, 엘엘씨 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산

Also Published As

Publication number Publication date
US20160326499A1 (en) 2016-11-10
US20180135028A1 (en) 2018-05-17
US20200109374A1 (en) 2020-04-09
KR20210095226A (ko) 2021-07-30
KR20170113710A (ko) 2017-10-12
AU2013243602B2 (en) 2016-11-24
MX2014011903A (es) 2015-05-12
US20150064757A1 (en) 2015-03-05
EP2834350B1 (en) 2016-11-16
CN104704113B (zh) 2018-01-23
SI3385374T1 (sl) 2020-09-30
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
EP3385374B1 (en) 2020-01-08
EP3674399A1 (en) 2020-07-01
MX358177B (es) 2018-08-08
CO7170148A2 (es) 2015-01-28
JP2015512645A (ja) 2015-04-30
KR102185762B1 (ko) 2020-12-02
BR122019026987B1 (pt) 2022-01-18
BR112014024674B1 (pt) 2022-06-07
KR20200038559A (ko) 2020-04-13
MX343296B (es) 2016-11-01
EP3153579B1 (en) 2018-03-14
EP3985108A1 (en) 2022-04-20
KR20190022935A (ko) 2019-03-06
AU2013243602A1 (en) 2014-11-13
KR20190076059A (ko) 2019-07-01
ES2786633T3 (es) 2020-10-13
AU2016231497A1 (en) 2016-10-06
US10301603B2 (en) 2019-05-28
CA2883064A1 (en) 2013-10-10
BR112014024674A2 (pt) 2018-11-21
EP2834350A2 (en) 2015-02-11
AU2016231497B2 (en) 2017-08-31
MY170889A (en) 2019-09-12
KR101831331B1 (ko) 2018-02-23
KR20150032660A (ko) 2015-03-27
WO2013152052A2 (en) 2013-10-10
EP3385374A1 (en) 2018-10-10
ES2613553T3 (es) 2017-05-24
US9340801B2 (en) 2016-05-17
EP3153579A1 (en) 2017-04-12
WO2013152052A3 (en) 2014-03-13
KR102282677B1 (ko) 2021-07-28
US11034937B2 (en) 2021-06-15
EP4186974A1 (en) 2023-05-31
KR102114841B1 (ko) 2020-05-26
CN104704113A (zh) 2015-06-10
US9758769B2 (en) 2017-09-12

Similar Documents

Publication Publication Date Title
JP6230594B2 (ja) Ｃａｒ酵素、および改良された脂肪アルコール産生方法
JP6738852B2 (ja) 改良された脂肪酸誘導体産生方法
JP7094343B2 (ja) 改良された特性を有するアシル－ａｃｐレダクターゼ
JP2016500261A (ja) 脂肪酸誘導体のａｃｐ媒介性生産方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150108

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20150209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150209

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160224

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20160523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20160523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160805

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160805

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20160817

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160829

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161128

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161226

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170213

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170512

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170809

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170927

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20171017

R150 Certificate of patent or registration of utility model

Ref document number: 6230594

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250