KR900008740B1 - 혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법 - Google Patents

혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법 Download PDF

Info

Publication number
KR900008740B1
KR900008740B1 KR1019830006314A KR830006314A KR900008740B1 KR 900008740 B1 KR900008740 B1 KR 900008740B1 KR 1019830006314 A KR1019830006314 A KR 1019830006314A KR 830006314 A KR830006314 A KR 830006314A KR 900008740 B1 KR900008740 B1 KR 900008740B1
Authority
KR
South Korea
Prior art keywords
tpa
serum
cells
pro
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
KR1019830006314A
Other languages
English (en)
Korean (ko)
Other versions
KR840006825A (ko
Inventor
리네테 윌슨 엘라이네
Original Assignee
바이오-리스폰스, 인코포레이티드
다이안 앰. 도거티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 바이오-리스폰스, 인코포레이티드, 다이안 앰. 도거티 filed Critical 바이오-리스폰스, 인코포레이티드
Publication of KR840006825A publication Critical patent/KR840006825A/ko
Application granted granted Critical
Publication of KR900008740B1 publication Critical patent/KR900008740B1/ko
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1019830006314A 1982-12-30 1983-12-30 혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법 Expired KR900008740B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
ZA9576 1982-12-30
ZA829576 1982-12-30
ZA4576 1983-06-22
ZA834576 1983-06-22
ZA82/9576 1983-06-22

Publications (2)

Publication Number Publication Date
KR840006825A KR840006825A (ko) 1984-12-03
KR900008740B1 true KR900008740B1 (ko) 1990-11-29

Family

ID=27134272

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019830006314A Expired KR900008740B1 (ko) 1982-12-30 1983-12-30 혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법

Country Status (17)

Country Link
US (2) US4798796A (enExample)
EP (1) EP0113319B1 (enExample)
JP (3) JPH0632607B2 (enExample)
KR (1) KR900008740B1 (enExample)
AU (1) AU579812B2 (enExample)
CA (1) CA1267620A (enExample)
DE (1) DE3382390D1 (enExample)
DK (1) DK607083A (enExample)
ES (2) ES528570A0 (enExample)
FI (1) FI81377C (enExample)
GR (1) GR78779B (enExample)
HU (1) HU197766B (enExample)
IL (1) IL70573A (enExample)
NO (1) NO169497C (enExample)
NZ (1) NZ206699A (enExample)
PH (1) PH26514A (enExample)
PT (1) PT77897B (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA831399B (en) * 1982-03-05 1984-02-29 Health Lab Service Board New fibrinolytic enzymes and methods for their production and pharmaceutical compositions containing them
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
JP2648301B2 (ja) * 1983-12-27 1997-08-27 ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド 真核細胞の形質転換のための補助ｄｎａを含むベクター
US5079159A (en) * 1983-12-27 1992-01-07 Genetics Institute, Inc. Method for making tissue plasminogen activator
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
DE3531966A1 (de) * 1985-09-07 1987-03-12 Arnim Ulrich Christoph Von Verfahren zur langzeit-zuechtung beliebiger endothelzellen, methode zu ihrer identifizierung und charakterisierung und ihre verwendung fuer diagnose und klaerung der aetiopatogenese von krankheitszustaenden
US4889808A (en) * 1985-10-01 1989-12-26 American Home Products Method of enchancing t-PA and SCU-PA production
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
JPS63196268A (ja) * 1987-02-10 1988-08-15 Kanegafuchi Chem Ind Co Ltd 無血清培地で継代増殖可能な形質転換細胞、その育種方法およびその細胞による蛋白質の生産方法
WO1989000605A1 (en) * 1987-07-16 1989-01-26 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
JPS6427472A (en) * 1987-07-21 1989-01-30 Meiji Milk Prod Co Ltd Production of human tissue plasminogen activator and cell strain using therein
US5128449A (en) * 1988-07-05 1992-07-07 The University Of Tennessee Research Corporation Polypeptide and a method for its production
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
FR2657884B1 (fr) * 1990-02-05 1994-09-02 Tm Innovation Procede pour la preparation du facteur viii humain et d'analogues du facteur viii.
US5141862A (en) * 1990-04-17 1992-08-25 Smithkline Beecham Corporation Method of purifying tpa or plasminogen activator using a tripeptide of the formula: -X-Y-argininal wherein X and Y are selected from the group consisting of pro,phe,trp and tyr
US5227297A (en) * 1990-04-17 1993-07-13 Smithkline Beecham Corporation Affinity purification ligands
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US5753489A (en) * 1994-11-10 1998-05-19 Immuno Ag Method for producing viruses and vaccines in serum-free culture
US5756341A (en) * 1994-11-10 1998-05-26 Immuno Ag Method for controlling the infectivity of viruses
US6146873A (en) * 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
US5698433A (en) * 1994-11-10 1997-12-16 Immuno Ag Method for producing influenza virus and vaccine
US5849585A (en) * 1995-05-10 1998-12-15 Genetech, Inc. Isolating and culturing Schwann cells
US5721139A (en) * 1995-05-10 1998-02-24 Genentech, Inc. Isolating and culturing schwann cells
US5714385A (en) * 1995-05-10 1998-02-03 Genentech, Inc. Media for culturing schwann cells
US6033660A (en) * 1995-05-10 2000-03-07 Genentech, Inc. Method of treating a nervous system injury with cultured schwann cells
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
EP2121903A2 (en) * 2007-02-16 2009-11-25 Fondazione Centro San Raffaele del Monte Tabor Mitogen independence identifies a highly malignant population of tumor stem cells
JP4891946B2 (ja) * 2008-05-19 2012-03-07 大成ロテック株式会社 防水層の施工方法、アスファルトパネルの製造方法及びアスファルトパネル用型枠

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
JPS5774085A (en) * 1980-10-25 1982-05-10 Ajinomoto Co Inc Culture medium for cells originating from lymphocytes
US4448879A (en) * 1981-03-26 1984-05-15 Hooper Trading Company High yield process for in vitro production of serum-free and mitogen-free interleukin-2
US4517293A (en) * 1981-03-26 1985-05-14 Hooper Trading Co. N.V. High yield process for in vitro production of serum-free and mitogen-free interleukin-2 using a roller bottle culture system
US4544632A (en) * 1981-06-12 1985-10-01 Yuichi Yamamura Human T cell lines and method of producing same
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
JPS59169489A (ja) * 1983-03-15 1984-09-25 Otsuka Pharmaceut Co Ltd ヒト培養株化細胞
EP0151579B1 (en) * 1983-04-27 1995-09-06 The President And Fellows Of Harvard College Method and products for detection of human t cell leukemia virus

Also Published As

Publication number Publication date
FI834830A0 (fi) 1983-12-28
US4798796A (en) 1989-01-17
NO169497B (no) 1992-03-23
IL70573A0 (en) 1984-03-30
NO169497C (no) 1992-07-01
EP0113319A2 (en) 1984-07-11
ES8600389A1 (es) 1985-10-01
FI81377C (fi) 1990-10-10
FI834830L (fi) 1984-07-01
AU579812B2 (en) 1988-12-15
NZ206699A (en) 1989-08-29
DK607083A (da) 1984-07-01
PH26514A (en) 1992-08-07
PT77897B (en) 1986-05-08
EP0113319A3 (en) 1987-04-08
EP0113319B1 (en) 1991-08-28
NO834866L (no) 1984-07-02
JPH0632607B2 (ja) 1994-05-02
DE3382390D1 (de) 1991-10-02
AU2296383A (en) 1984-07-05
HU197766B (en) 1989-05-29
JPH0670766A (ja) 1994-03-15
IL70573A (en) 1989-09-10
US5147790A (en) 1992-09-15
PT77897A (en) 1984-01-01
GR78779B (enExample) 1984-10-02
ES528570A0 (es) 1985-10-01
CA1267620A (en) 1990-04-10
ES543559A0 (es) 1986-04-01
FI81377B (fi) 1990-06-29
ES8606396A1 (es) 1986-04-01
JPS59139324A (ja) 1984-08-10
KR840006825A (ko) 1984-12-03
JPH08205859A (ja) 1996-08-13
JP2534020B2 (ja) 1996-09-11
DK607083D0 (da) 1983-12-29

Similar Documents

Publication Publication Date Title
KR900008740B1 (ko) 혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법
EP0005644B1 (en) Production of urokinase
US4780412A (en) Fibrinolytic enzymes produced from established non-cancerous cell lines
KR100508043B1 (ko) rDSPAα1의 제조방법
KR20060055302A (ko) 혈액 점도 저하제
KR900002345B1 (ko) 정상인의 결장세포로 부터 플라스미노겐 활성인자의 대량 생산방법
Murakami et al. Renin precursor and its activation mechanism in hog kidney
CN101134951A (zh) 一种纤溶酶及其培制方法
PT88751B (pt) Processo para a preparacao de proteinas
WO1990001333A1 (en) METHOD FOR PREPARING tPA COMPOSITIONS
CA1275062A (en) Plasminogen activator kym
CA2245554C (en) Method for the production of rdspa .alpha.1
KR980009454A (ko) 바실러스속 균주 유래의 혈전용해효소
Fulton et al. Morphological transformation of cells induced by Kirsten sarcoma virus transforming factor is independent of serine protease
DD251995A5 (de) Verfahren zur Herstellung von Gewebeplasminogenaktivatoren
JPS62205784A (ja) 新しい型のプラスミノ−ゲン活性化因子の製造方法
DD234279A5 (de) Verfahren zur herstellung einer serumunabhaengigen humanzellinie
JPS61271987A (ja) ウロキナ−ゼ前駆体の製造方法
Clark et al. 4868119 Hematopoietic growth factors
Jaye et al. 4868113 Recombinant DNA vector encoding human endothelial cell growth factor
JPH02312588A (ja) 細胞の培養方法及びプロテアーゼの製造法
JPH01256385A (ja) 組織型プラスミノーゲン活性化因子の製造法
JPH0532025B2 (enExample)

Legal Events

Date Code Title Description
PA0109 Patent application

St.27 status event code: A-0-1-A10-A12-nap-PA0109

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

N231 Notification of change of applicant
PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

A201 Request for examination
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

G160 Decision to publish patent application
PG1605 Publication of application before grant of patent

St.27 status event code: A-2-2-Q10-Q13-nap-PG1605

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U11-oth-PR1002

Fee payment year number: 1

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

FPAY Annual fee payment

Payment date: 19951103

Year of fee payment: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

LAPS Lapse due to unpaid annual fee
PC1903 Unpaid annual fee

St.27 status event code: A-4-4-U10-U13-oth-PC1903

Not in force date: 19961130

Payment event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

PC1903 Unpaid annual fee

St.27 status event code: N-4-6-H10-H13-oth-PC1903

Ip right cessation event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

Not in force date: 19961130