ES8606396A1 - Procedimiento para la preparacion de activadores de plasmi- nogeno de tejidos - Google Patents

Procedimiento para la preparacion de activadores de plasmi- nogeno de tejidos

Info

Publication number
ES8606396A1
ES8606396A1 ES543559A ES543559A ES8606396A1 ES 8606396 A1 ES8606396 A1 ES 8606396A1 ES 543559 A ES543559 A ES 543559A ES 543559 A ES543559 A ES 543559A ES 8606396 A1 ES8606396 A1 ES 8606396A1
Authority
ES
Spain
Prior art keywords
proteins
cell lines
production
serum
proliferate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543559A
Other languages
English (en)
Other versions
ES543559A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WILSON ELAINE LYN
Original Assignee
WILSON ELAINE LYN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WILSON ELAINE LYN filed Critical WILSON ELAINE LYN
Publication of ES543559A0 publication Critical patent/ES543559A0/es
Publication of ES8606396A1 publication Critical patent/ES8606396A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE ACTIVADORES DE PLASMINOGENO DE TEJIDOS. CONSISTE EN CULTIVAR CELULAS HUMANAS INDEPENDIENTEMENTE DE SUERO, TAL COMO SON LAS CELULAS BOWES II O MUTANTES DE LAS MISMAS, CAPACES DE PRODUCIR TPA Y/O PRO-TPA, EN UN MEDIO LIBRE DE SUERO Y AISLAR LAS PROTEINAS DESEADAS DEL FLUIDO DE COSECHA Y, SI SE DESEA, LA MEZCLA TPA O PRO-TPA OBTENIDA SE SEPARA O SE CONVIERTE EN LOS COMPONENTES INDIVIDUALES; DONDE LA PREPARACION DE TPA LIBRE DEPRO-TPA SE OBTIENE CONVIRTIENDO ENZIMATICAMENTE EL PRO-TPA EN TPA, INCLUYENDOSE UN INHIBIDOR DE PROTEASA DURANTE EL PROCEDIMIENTO, DE AISLAMIENTO Y PURIFICACION, Y LA PURIFICACION FINAL SE EFECTUA EN PRESENCIA DE UN INHIBIDOR QUE INHIBE SELECTIVAMENTE TPA, RESULTANDO UNA MEZCLA DE ACTIVADORES DE PLASMINOGENOS DE TEJIDOS EN DONDE EL 90% ESTA EN FORMA PRO-ENZIMATICA Y EL 10% EN FORMA ENZIMATICAMENTE ACTIVA.
ES543559A 1982-12-30 1985-05-28 Procedimiento para la preparacion de activadores de plasmi- nogeno de tejidos Expired ES8606396A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA829576 1982-12-30
ZA834576 1983-06-22

Publications (2)

Publication Number Publication Date
ES543559A0 ES543559A0 (es) 1986-04-01
ES8606396A1 true ES8606396A1 (es) 1986-04-01

Family

ID=27134272

Family Applications (2)

Application Number Title Priority Date Filing Date
ES528570A Expired ES8600389A1 (es) 1982-12-30 1983-12-30 Procedimiento para la produccion de una linea celular humana independiente de suero
ES543559A Expired ES8606396A1 (es) 1982-12-30 1985-05-28 Procedimiento para la preparacion de activadores de plasmi- nogeno de tejidos

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES528570A Expired ES8600389A1 (es) 1982-12-30 1983-12-30 Procedimiento para la produccion de una linea celular humana independiente de suero

Country Status (17)

Country Link
US (2) US4798796A (es)
EP (1) EP0113319B1 (es)
JP (3) JPH0632607B2 (es)
KR (1) KR900008740B1 (es)
AU (1) AU579812B2 (es)
CA (1) CA1267620A (es)
DE (1) DE3382390D1 (es)
DK (1) DK607083A (es)
ES (2) ES8600389A1 (es)
FI (1) FI81377C (es)
GR (1) GR78779B (es)
HU (1) HU197766B (es)
IL (1) IL70573A (es)
NO (1) NO169497C (es)
NZ (1) NZ206699A (es)
PH (1) PH26514A (es)
PT (1) PT77897B (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA831399B (en) * 1982-03-05 1984-02-29 Health Lab Service Board New fibrinolytic enzymes and methods for their production and pharmaceutical compositions containing them
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
US5079159A (en) * 1983-12-27 1992-01-07 Genetics Institute, Inc. Method for making tissue plasminogen activator
JP2648301B2 (ja) * 1983-12-27 1997-08-27 ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド 真核細胞の形質転換のための補助ｄｎａを含むベクター
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
DE3531966A1 (de) * 1985-09-07 1987-03-12 Arnim Ulrich Christoph Von Verfahren zur langzeit-zuechtung beliebiger endothelzellen, methode zu ihrer identifizierung und charakterisierung und ihre verwendung fuer diagnose und klaerung der aetiopatogenese von krankheitszustaenden
US4889808A (en) * 1985-10-01 1989-12-26 American Home Products Method of enchancing t-PA and SCU-PA production
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc A process for producing a biologically active tissue plasminogen activator
JPS63196268A (ja) * 1987-02-10 1988-08-15 Kanegafuchi Chem Ind Co Ltd 無血清培地で継代増殖可能な形質転換細胞、その育種方法およびその細胞による蛋白質の生産方法
WO1989000605A1 (en) * 1987-07-16 1989-01-26 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
JPS6427472A (en) * 1987-07-21 1989-01-30 Meiji Milk Prod Co Ltd Production of human tissue plasminogen activator and cell strain using therein
US5128449A (en) * 1988-07-05 1992-07-07 The University Of Tennessee Research Corporation Polypeptide and a method for its production
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
FR2657884B1 (fr) * 1990-02-05 1994-09-02 Tm Innovation Procede pour la preparation du facteur viii humain et d'analogues du facteur viii.
US5141862A (en) * 1990-04-17 1992-08-25 Smithkline Beecham Corporation Method of purifying tpa or plasminogen activator using a tripeptide of the formula: -X-Y-argininal wherein X and Y are selected from the group consisting of pro,phe,trp and tyr
US5227297A (en) * 1990-04-17 1993-07-13 Smithkline Beecham Corporation Affinity purification ligands
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US5698433A (en) * 1994-11-10 1997-12-16 Immuno Ag Method for producing influenza virus and vaccine
US5756341A (en) * 1994-11-10 1998-05-26 Immuno Ag Method for controlling the infectivity of viruses
US6146873A (en) * 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
US5753489A (en) * 1994-11-10 1998-05-19 Immuno Ag Method for producing viruses and vaccines in serum-free culture
US6033660A (en) * 1995-05-10 2000-03-07 Genentech, Inc. Method of treating a nervous system injury with cultured schwann cells
US5849585A (en) * 1995-05-10 1998-12-15 Genetech, Inc. Isolating and culturing Schwann cells
US5721139A (en) * 1995-05-10 1998-02-24 Genentech, Inc. Isolating and culturing schwann cells
US5714385A (en) * 1995-05-10 1998-02-03 Genentech, Inc. Media for culturing schwann cells
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
EP2121903A2 (en) * 2007-02-16 2009-11-25 Fondazione Centro San Raffaele del Monte Tabor Mitogen independence identifies a highly malignant population of tumor stem cells
JP4891946B2 (ja) * 2008-05-19 2012-03-07 大成ロテック株式会社 防水層の施工方法、アスファルトパネルの製造方法及びアスファルトパネル用型枠

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
JPS5774085A (en) * 1980-10-25 1982-05-10 Ajinomoto Co Inc Culture medium for cells originating from lymphocytes
US4517293A (en) * 1981-03-26 1985-05-14 Hooper Trading Co. N.V. High yield process for in vitro production of serum-free and mitogen-free interleukin-2 using a roller bottle culture system
US4448879A (en) * 1981-03-26 1984-05-15 Hooper Trading Company High yield process for in vitro production of serum-free and mitogen-free interleukin-2
US4544632A (en) * 1981-06-12 1985-10-01 Yuichi Yamamura Human T cell lines and method of producing same
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
JPS59169489A (ja) * 1983-03-15 1984-09-25 Otsuka Pharmaceut Co Ltd ヒト培養株化細胞
WO1984004327A1 (en) * 1983-04-27 1984-11-08 Harvard College Method and products for detection of human t cell leukemia virus

Also Published As

Publication number Publication date
CA1267620A (en) 1990-04-10
AU2296383A (en) 1984-07-05
ES528570A0 (es) 1985-10-01
JPH08205859A (ja) 1996-08-13
KR840006825A (ko) 1984-12-03
FI834830A (fi) 1984-07-01
IL70573A (en) 1989-09-10
DE3382390D1 (de) 1991-10-02
JPH0670766A (ja) 1994-03-15
US5147790A (en) 1992-09-15
EP0113319A2 (en) 1984-07-11
KR900008740B1 (ko) 1990-11-29
FI834830A0 (fi) 1983-12-28
AU579812B2 (en) 1988-12-15
ES543559A0 (es) 1986-04-01
US4798796A (en) 1989-01-17
NO169497C (no) 1992-07-01
JP2534020B2 (ja) 1996-09-11
PT77897A (en) 1984-01-01
ES8600389A1 (es) 1985-10-01
DK607083D0 (da) 1983-12-29
DK607083A (da) 1984-07-01
EP0113319A3 (en) 1987-04-08
EP0113319B1 (en) 1991-08-28
PH26514A (en) 1992-08-07
IL70573A0 (en) 1984-03-30
FI81377B (fi) 1990-06-29
NO834866L (no) 1984-07-02
NZ206699A (en) 1989-08-29
GR78779B (es) 1984-10-02
NO169497B (no) 1992-03-23
JPH0632607B2 (ja) 1994-05-02
PT77897B (en) 1986-05-08
FI81377C (fi) 1990-10-10
HU197766B (en) 1989-05-29
JPS59139324A (ja) 1984-08-10

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20001102