KR840006825A - 세포라인의 제조방법 - Google Patents

세포라인의 제조방법 Download PDF

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KR840006825A
KR840006825A KR1019830006314A KR830006314A KR840006825A KR 840006825 A KR840006825 A KR 840006825A KR 1019830006314 A KR1019830006314 A KR 1019830006314A KR 830006314 A KR830006314 A KR 830006314A KR 840006825 A KR840006825 A KR 840006825A
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serum
tpa
cell line
incubator
cells
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KR900008740B1 (ko
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리네테 윌슨 엘라이네
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리네테 윌슨 엘라이네
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification

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  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

세포라인의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (26)

  1. 혈청-의존성 인간세포라인의 배양물로부터 혈청함유 배양기를 제거하고 대신에 혈청이 없는 배양기를 대치하고, 착생되거나 착생되지 않은 세포에 혈청이 없는 배양기를 공급하고, 혈청-비의존성 세포라인이 될 때까지 세포를 성장하게 하고, 필요시 수득한 혈청-비의존성 세포라인으로부터 혈청-비의존성돌연변이 세포라인을 생성함을 특징으로 하고, 혈청-비의존성 인간세포라인 또는 이로부터 유도된 혈청-비의존성 돌연변이 세포라인을 제조하는 방법.
  2. 제1항에 있어서, 혈청이 없는 배양기가 조절된 배양기이고, 조절된 배양기를 더 이상 필요로 하지 않을 때까지, 세포를 성장하게 하는 방법.
  3. 제2항에 있어서, 혈청이 없는 조절된 배양기를, 동료혈청-의존성 세포배양물로부터 수득하는 방법.
  4. 제1항에 있어서, 배양기 부재하에 세포를 약 30%이상으로 성장하게 하는 방법.
  5. 제1항에 있어서, 비-착생된 세포를 함유하는 배양기를 분리하고, 배양기로부터 비-착생된 세포를 분리하고, 이를 새로운 혈청이 없는 배양기와 함께 착생된 세포에 다시 가하여, 혈청이 없는 배양기를 제조하는 방법.
  6. 제1항에 있어서, 혈청이 없는 배양기의 일부분을, 24내지 72시간 간격으로, 새로운 혈청이 없는 배양기로 대치시켜, 혈청이 없는 배양기를 제조하는 방법.
  7. 제1항 내지 제6항중의 어느 하나에 있어서, 혈청-의존성 세포라인이 인간흑색종 세포라인인 방법.
  8. 제1항 내지 제6항중의 어느 하나에 있어서, 혈청-의존성 세포란인이 TPA 및 / 또는 프로-TPA를 생성하는 세포라인인 방법.
  9. 제1항 내지 제6항중의 어느 하나에 있어서, 혈청-의존성 세포란인이 보우스 I 세포라인인 방법.
  10. 제1항에 있어서, TPA 및/또는 프로-TPA를 생성하는 혈청-비의존성 세포라인 또는 이로부터 유도된 돌연변이 세포라인을 제조하는 방법.
  11. 제1항에 있어서, 보우스II 세포라인 또는 이로부터 유도된 혈청-비의존성 돌연변이 세포라인을 제조하는 방법.
  12. 제1항에 있어서, 상응하는 혈청-비의존성 돌연변이 세포라인을 제조하기 위해 수득한 혈청-비의존성 세포라인을 돌연변이원으로 처리하거나 조사(照射)시키는 방법.
  13. TPA 및/또는 프로-TPA를 생성할 수 있는 혈청-비의존성 인간세포를, 혈청이 없는 배양기중에서 배양하고, 목적하는 단백질을 하베스트 유체로부터 분리하고, 필요시 수득한 TPA 및 프로-TPA의 혼합물을 개개성분으로 분리 또는 전환시킴을 특징으로 하여, TPA, 프로-TPA 또는 이들의 혼합물을 제조하는 방법.
  14. 제13항에 있어서, 혈청-비의존성 인간세포가 보우스II 세포 또는 이의 돌연변이청인 방법.
  15. 제13항에 있어서, 수득한 혼합물중에 존재하는 프로-TPA를 효소적으로 TPA로 전환시킴을 특징으로 하여, 프로-TPA가 없는 TPA를 제조하는 방법.
  16. 제13항에 있어서, 분리 및 정제공정동안 프로테아제 억제제가 포함되어 있고, TPA를 선택적으로 억제하는 억제제의 존재하에서 최종정제를 수행함을 특징으로 하여, TPA가 없는 프로-TPA를 제조하는 방법.
  17. 제13항에 있어서, 조직 플라스미노겐 활성인자(TPA)와 프로-조직 플라스미노겐 활성인자(프로-TPA)의 혼합물을 제조하는 방법.
  18. 제17항에 있어서, 90%가 전-효소(proenzyme)형태이고, 10%가 활성효소형태인 조직플라스미노겐 활성인자(TPA)의 혼합물을 제조하는 방법.
  19. 제15항에 있어서, 수득한 TPA와 프로-TPA의 혼합물을, 플라스민이나 프로-TPA를 동일한 효과를 가지는 효소로 처리하는 것을 특징으로 하는 방법.
  20. 제16항에 있어서, 프로테아제 억제제가 아프로티닌 및 염기성 판크레아틱 트립신 억제제중에는 선택되는 방법.
  21. 제16항 및 20항중의 어느 하나에 있어서, 디이소프로필풀루오로포스페이트 또는 니트로페닐구아니디노벤조에이트의 존재하에, 선택적인 어피니티시약을 함유하는 칼럼상에서 크로마토그라피하여, 최종정제를 수행하는 방법.
  22. 제13항에 있어서, 모노클로날 안티-TPA항체 칼럼상에서 크로마토그라피하여, 최종정제를 수행하는 방법.
  23. 제13항에 있어서, 세포를 착생배양하는 방법.
  24. 제13항에 있어서, 세포를 현탁액 배양으로서 배양하는 방법.
  25. 제13항에 있어서, 수득한 하베스트 유체를 원심분리한 후, 어피니티 크로마토그라피시키는 방법.
  26. 제13항에 있어서, 분리 및 정제공정동안, 세제가 포함되어 있는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019830006314A 1982-12-30 1983-12-30 혈청-비의존성 사람 세포주 및 이의 돌연변이 세포주를 제조하는 방법 KR900008740B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
ZA82/9576 1982-12-30
ZA829576 1982-12-30
ZA9576 1982-12-30
ZA4576 1983-06-22
ZA834576 1983-06-22

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KR840006825A true KR840006825A (ko) 1984-12-03
KR900008740B1 KR900008740B1 (ko) 1990-11-29

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US (2) US4798796A (ko)
EP (1) EP0113319B1 (ko)
JP (3) JPH0632607B2 (ko)
KR (1) KR900008740B1 (ko)
AU (1) AU579812B2 (ko)
CA (1) CA1267620A (ko)
DE (1) DE3382390D1 (ko)
DK (1) DK607083A (ko)
ES (2) ES8600389A1 (ko)
FI (1) FI81377C (ko)
GR (1) GR78779B (ko)
HU (1) HU197766B (ko)
IL (1) IL70573A (ko)
NO (1) NO169497C (ko)
NZ (1) NZ206699A (ko)
PH (1) PH26514A (ko)
PT (1) PT77897B (ko)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA831399B (en) * 1982-03-05 1984-02-29 Health Lab Service Board New fibrinolytic enzymes and methods for their production and pharmaceutical compositions containing them
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
JP2648301B2 (ja) * 1983-12-27 1997-08-27 ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド 真核細胞の形質転換のための補助ｄｎａを含むベクター
US5079159A (en) * 1983-12-27 1992-01-07 Genetics Institute, Inc. Method for making tissue plasminogen activator
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
DE3531966A1 (de) * 1985-09-07 1987-03-12 Arnim Ulrich Christoph Von Verfahren zur langzeit-zuechtung beliebiger endothelzellen, methode zu ihrer identifizierung und charakterisierung und ihre verwendung fuer diagnose und klaerung der aetiopatogenese von krankheitszustaenden
US4889808A (en) * 1985-10-01 1989-12-26 American Home Products Method of enchancing t-PA and SCU-PA production
PT84991B (pt) * 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo
JPS63196268A (ja) * 1987-02-10 1988-08-15 Kanegafuchi Chem Ind Co Ltd 無血清培地で継代増殖可能な形質転換細胞、その育種方法およびその細胞による蛋白質の生産方法
EP0368926A4 (en) * 1987-07-16 1991-11-27 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
JPS6427472A (en) * 1987-07-21 1989-01-30 Meiji Milk Prod Co Ltd Production of human tissue plasminogen activator and cell strain using therein
US5128449A (en) * 1988-07-05 1992-07-07 The University Of Tennessee Research Corporation Polypeptide and a method for its production
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
FR2657884B1 (fr) * 1990-02-05 1994-09-02 Tm Innovation Procede pour la preparation du facteur viii humain et d'analogues du facteur viii.
US5227297A (en) * 1990-04-17 1993-07-13 Smithkline Beecham Corporation Affinity purification ligands
US5141862A (en) * 1990-04-17 1992-08-25 Smithkline Beecham Corporation Method of purifying tpa or plasminogen activator using a tripeptide of the formula: -X-Y-argininal wherein X and Y are selected from the group consisting of pro,phe,trp and tyr
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US5753489A (en) * 1994-11-10 1998-05-19 Immuno Ag Method for producing viruses and vaccines in serum-free culture
US6146873A (en) * 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
US5698433A (en) * 1994-11-10 1997-12-16 Immuno Ag Method for producing influenza virus and vaccine
US5756341A (en) * 1994-11-10 1998-05-26 Immuno Ag Method for controlling the infectivity of viruses
US5849585A (en) * 1995-05-10 1998-12-15 Genetech, Inc. Isolating and culturing Schwann cells
US6033660A (en) * 1995-05-10 2000-03-07 Genentech, Inc. Method of treating a nervous system injury with cultured schwann cells
US5721139A (en) * 1995-05-10 1998-02-24 Genentech, Inc. Isolating and culturing schwann cells
US5714385A (en) * 1995-05-10 1998-02-03 Genentech, Inc. Media for culturing schwann cells
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
WO2008099006A2 (en) * 2007-02-16 2008-08-21 Fondazione Centro San Raffaele Del Monte Tabor Mitogen independence identifies a highly malignant population of tumor stem cells
JP4891946B2 (ja) * 2008-05-19 2012-03-07 大成ロテック株式会社 防水層の施工方法、アスファルトパネルの製造方法及びアスファルトパネル用型枠

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
NL8003402A (nl) * 1980-06-11 1982-01-04 Leuven Res & Dev Vzw Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking.
JPS5774085A (en) * 1980-10-25 1982-05-10 Ajinomoto Co Inc Culture medium for cells originating from lymphocytes
US4448879A (en) * 1981-03-26 1984-05-15 Hooper Trading Company High yield process for in vitro production of serum-free and mitogen-free interleukin-2
US4517293A (en) * 1981-03-26 1985-05-14 Hooper Trading Co. N.V. High yield process for in vitro production of serum-free and mitogen-free interleukin-2 using a roller bottle culture system
US4544632A (en) * 1981-06-12 1985-10-01 Yuichi Yamamura Human T cell lines and method of producing same
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
JPS59169489A (ja) * 1983-03-15 1984-09-25 Otsuka Pharmaceut Co Ltd ヒト培養株化細胞
EP0151579B1 (en) * 1983-04-27 1995-09-06 The President And Fellows Of Harvard College Method and products for detection of human t cell leukemia virus

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DE3382390D1 (de) 1991-10-02
ES8606396A1 (es) 1986-04-01
IL70573A0 (en) 1984-03-30
JP2534020B2 (ja) 1996-09-11
US5147790A (en) 1992-09-15
EP0113319A3 (en) 1987-04-08
EP0113319B1 (en) 1991-08-28
ES528570A0 (es) 1985-10-01
JPS59139324A (ja) 1984-08-10
IL70573A (en) 1989-09-10
AU2296383A (en) 1984-07-05
JPH08205859A (ja) 1996-08-13
FI834830A0 (fi) 1983-12-28
PT77897B (en) 1986-05-08
EP0113319A2 (en) 1984-07-11
DK607083D0 (da) 1983-12-29
NO169497B (no) 1992-03-23
HU197766B (en) 1989-05-29
ES8600389A1 (es) 1985-10-01
NZ206699A (en) 1989-08-29
FI81377C (fi) 1990-10-10
ES543559A0 (es) 1986-04-01
NO834866L (no) 1984-07-02
CA1267620A (en) 1990-04-10
KR900008740B1 (ko) 1990-11-29
FI81377B (fi) 1990-06-29
JPH0632607B2 (ja) 1994-05-02
JPH0670766A (ja) 1994-03-15
PT77897A (en) 1984-01-01
US4798796A (en) 1989-01-17
GR78779B (ko) 1984-10-02
NO169497C (no) 1992-07-01
DK607083A (da) 1984-07-01
AU579812B2 (en) 1988-12-15
FI834830A (fi) 1984-07-01
PH26514A (en) 1992-08-07

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