KR890701735A - 할로아릴니트릴 분해 유전자, 그의 용도 및 유전자를 함유하는 세포 - Google Patents

할로아릴니트릴 분해 유전자, 그의 용도 및 유전자를 함유하는 세포

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KR890701735A
KR890701735A KR1019890700419A KR890700419A KR890701735A KR 890701735 A KR890701735 A KR 890701735A KR 1019890700419 A KR1019890700419 A KR 1019890700419A KR 890700419 A KR890700419 A KR 890700419A KR 890701735 A KR890701735 A KR 890701735A
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bacterial
nitriase
specific
expression cassette
dna
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KR1019890700419A
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English (en)
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스타커 데이비드
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프랑스와 끄레띠엥
롱 쁠랑 아그로시미
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Publication of KR890701735A publication Critical patent/KR890701735A/ko

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

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  • Enzymes And Modification Thereof (AREA)
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Abstract

내용 없음

Description

할로아릴니트릴 분해 유전자, 그의 용도 및 유전자를 함유하는 세포
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (22)

  1. 박테리아 니트릴라제가 치환, 자름 또는 확장중 적어도 하나에 의해 변형되며 전체로 약 50개의 아미노산 이하로 구성되어 있는, 기질로서 브로목시닐을 가지며 실질적으로 3,5-디할로겐화-p-히드록시벤조니트릴에 대하여 특이적이며 적어도 약 0.1μmol NH3/분/㎎ 단백질의 특이 활성을 가지는 약 34KD의 실질적으로 순수 변형한 박테리아 니트릴라제.
  2. 제1항에 있어서, 박테리아 니트릴라제가 기질로서 브로목시닐을 가지며 적어도 약 0.1μmolNH3/분/㎎ 단백질의 특이활성을 가지는 실질적으로 순수 변형한 박테리아 니트릴라제.
  3. 제1항에 있어서, 박테리아 니트릴라제가 적어도 약 0.5μmol NH3/분/㎎ 단백질의 특이활성을 가지는 실질적으로 순수 변형한 박테리아 니트릴라제.
  4. 제1항에 있어서, 박테리아 니트릴라제가 클레브시엘라로부터 유래한 니트릴라제인 실질적으로 순수 변형한 박테리아 니트릴라제.
  5. 3,5-디할로겐화-p-히드록시벤조니트릴에 대하여 특이적인 실질적으로 순수 변형한 박테리아 니트릴라제를 발현하는 외래 유전자를 가지는 박테리아 숙주.
  6. 제5항에 있어서, 박테리아 숙주가 대장균인 박테리아 숙주.
  7. 니트릴라제가 기질로서 브로목시닐을 가지며 적어도 약 0.1μmol NH3/분/㎎ 단백질의 특이활성을 가지는, 식물 세포에서 기능적인 전사적 및 번역적 개시 조절 영역 및 제1항에 따른 실질적으로 순수 변형한 니트릴라제를 코오딩하는 유전자를 전사 방향으로 함유하는 발현 카셋트.
  8. 제7항에 있어서, 카셋트가 오른쪽 T-DNA 보더를 더 함유하는 발현 카셋트.
  9. 제7항에 있어서, 전사적 및 번역적 개시 조절영역이 식물 세포에서 기능성이 있는 발현 카셋트.
  10. 제9항에 있어서, 전사적 및 번역적 개시 조절영역이 오핀의 전사에 대한 조절영역인 발현 카셋트.
  11. 식물세포에서 기능적인 전사 및 번역 개시 조절영역 및 3,5-디할로겐화-p-히드록시벤조니트릴에 대하여 실질적으로 특이적이며 적어도 약 0.1μmol NH3/분/㎎ 단백질의 특이활성을 가지며 기질로서 브로목시닐을 갖는 실질적으로 순수 변형한 니트릴라젤르 코오딩하는 유전자를 전사방향으로 함유하는 발현 카셋트를 함유하는, 적어도 대장균 또는 에이 튜메파시엔스에서 안정적 유지를 할 수 있는 플라스미드.
  12. 제11항에 있어서, 발현 카셋트가 오른쪽 T-DNA 보더를 함유하는 플라스미드.
  13. 제11항에 있어서, 전사적 및 번역적 개시 조절 영역이 식물 세포에서 기능적인 플라스미드.
  14. 제13항에 있어서, 전사 및 번역 개시 조절 영역이 오핀 영역에서 기능적인 플라스미드.
  15. 제14항에 있어서, 전사 및 번역 개시 조절 영역이 만노핀 신타제 개시 조절 영역인 플라스미드.
  16. 실질적으로 3,5-디할로겐화-p-히드록시 벤조니트릴에 대하여 특이적이며 적어도 약 0.1μmol NH3/분/㎎ 단백질의 특이활성을 가지며 브로목시닐을 기질로서 갖는 실질적으로 순수 변형한 니트릴라제를 함유하는, 야생형 DNA 이외의 5＇ 또는 3＇말단 중 하나에 결합된 DNA 시퀸스.
  17. 제16항에 있어서, 개방 판독 프레임이 박테리아 DNA 인 DNA 시퀸스.
  18. 제17항에 있어서, 박테리아 DNA가 클래브시엘라 DNA 인 DNA 시퀸스.
  19. 제7 내지 9항중 어느 한 항에 따른 발현 카셋트를 함유하는 식물 세포.
  20. 제19항에 따른 식물 세포를 함유하는 식물체 부분.
  21. 제19항에 따른 식물세포를 함유하는 식물체.
  22. -3,5-디할로겐화-p-히드록시벤조니트릴에 대하여 특이적인 니트릴라제를 생성하는 케이 오자에나에를 분리하고-적절한 배지에서 상기 케이 오자에나에를 생장시키고-상기 케이 오자에나에를 용균하고 상기 니트릴라제를 분리함을 포함하는 3,54-디할로겐화-p-히드록시벤조니트릴에 대하여 특이적인 니트릴라제를 생성하는 방법.
    ※ 참고사항：최초출원 내용에 의하여 공개하는 것임.
KR1019890700419A 1987-07-08 1988-07-04 할로아릴니트릴 분해 유전자, 그의 용도 및 유전자를 함유하는 세포 KR890701735A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/071,146 US4810648A (en) 1986-01-08 1987-07-08 Haloarylnitrile degrading gene, its use, and cells containing the gene
US071,146 1987-07-08
PCT/EP1988/000588 WO1989000193A1 (en) 1987-07-08 1988-07-04 Haloarylnitrile degrading gene, its use, and cells containing the gene

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KR890701735A true KR890701735A (ko) 1989-12-21

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KR1019890700419A KR890701735A (ko) 1987-07-08 1988-07-04 할로아릴니트릴 분해 유전자, 그의 용도 및 유전자를 함유하는 세포

Country Status (15)

Country Link
US (1) US4810648A (ko)
EP (1) EP0373173A1 (ko)
KR (1) KR890701735A (ko)
CN (1) CN1031252A (ko)
AU (1) AU2071988A (ko)
BR (1) BR8807609A (ko)
CA (1) CA1339683C (ko)
DD (2) DD284048A5 (ko)
DK (1) DK2890A (ko)
ES (1) ES2010763A6 (ko)
GR (1) GR880100421A (ko)
IL (1) IL87001A (ko)
PL (1) PL273589A1 (ko)
WO (1) WO1989000193A1 (ko)
ZA (1) ZA884894B (ko)

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