KR20210151897A - 유전자 재조합 미생물 및 이를 이용한 목적 물질의 생산방법 - Google Patents

유전자 재조합 미생물 및 이를 이용한 목적 물질의 생산방법 Download PDF

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KR20210151897A
KR20210151897A KR1020217036600A KR20217036600A KR20210151897A KR 20210151897 A KR20210151897 A KR 20210151897A KR 1020217036600 A KR1020217036600 A KR 1020217036600A KR 20217036600 A KR20217036600 A KR 20217036600A KR 20210151897 A KR20210151897 A KR 20210151897A
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토오루 나카야시키
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그린 어스 인스티튜트 가부시키가이샤
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KR1020217036600A 2019-04-12 2019-09-06 유전자 재조합 미생물 및 이를 이용한 목적 물질의 생산방법 Withdrawn KR20210151897A (ko)

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JPJP-P-2019-076629 2019-04-12
JP2019076629 2019-04-12
PCT/JP2019/035286 WO2020208842A1 (ja) 2019-04-12 2019-09-06 遺伝子組換え微生物及びこれを用いた目的物質の生産方法

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US (1) US12600991B2 (https=)
EP (1) EP3954756A4 (https=)
JP (1) JP7360741B2 (https=)
KR (1) KR20210151897A (https=)
CN (2) CN112888776B (https=)
WO (1) WO2020208842A1 (https=)

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KR20240024116A (ko) 2021-06-29 2024-02-23 디아이씨 가부시끼가이샤 아스파라긴산 조성물, 폴리숙신이미드 조성물, 폴리아스파라긴산 조성물, 및 가교 폴리아스파라긴산 조성물
CN115678863B (zh) * 2021-07-28 2026-03-31 梅花(上海)生物科技有限公司 琥珀酸脱氢酶突变体、重组微生物及其制备方法和应用
JPWO2023068295A1 (https=) * 2021-10-21 2023-04-27
WO2023095896A1 (ja) * 2021-11-26 2023-06-01 Dic株式会社 遺伝子組換え微生物、及びアスパラギン酸を生産する方法
US20250092429A1 (en) * 2021-12-24 2025-03-20 Cj Cheiljedang Corporation 5'utr variant sequence of gene encoding phosphoenolpyruvate carboxylase, and uses thereof
CN116555251A (zh) * 2022-01-28 2023-08-08 廊坊梅花生物技术开发有限公司 一种生产苏氨酸的重组微生物及其应用
CN116606785A (zh) * 2022-02-08 2023-08-18 廊坊梅花生物技术开发有限公司 一种修饰的棒状杆菌属微生物及其应用与构建方法
CN116286364B (zh) * 2022-09-08 2023-11-10 杭州秀川科技有限公司 一种促进厌氧微生物分离和培养的复合物及其应用
KR102614733B1 (ko) * 2023-04-06 2023-12-19 대상 주식회사 포스포에놀피루베이트 카르복실라아제 신규 변이체 및 이를 이용한 5’-이노신산 생산 방법
CN118086355A (zh) * 2024-01-19 2024-05-28 稀物集(广州)生物科技有限公司 一种富集松茸活性成分的菌株及松茸发酵油的制备方法
WO2025254198A1 (ja) * 2024-06-06 2025-12-11 Ｇｒｅｅｎ Ｅａｒｔｈ Ｉｎｓｔｉｔｕｔｅ株式会社 標的物質を製造する方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0570860B2 (https=) 1985-11-08 1993-10-06 Nippon Electric Co
JP2003503064A (ja) 1999-06-29 2003-01-28 アーカー−ダニエルズ−ミッドランド カンパニー 炭素同化の調節
JP2006254730A (ja) 2005-03-15 2006-09-28 Univ Of Tokushima 新規なアスパラギン酸デヒドロゲナーゼ
JP2006254795A (ja) 2005-03-17 2006-09-28 Shimane Univ アスパラギン酸脱水素酵素、アラニン脱水素酵素、ｌ−アスパラギン酸製造方法、および、ｄ−リンゴ酸製造方法
JP2007043947A (ja) 2005-08-09 2007-02-22 Research Institute Of Innovative Technology For The Earth コリネ型細菌を用いる還元条件でのアミノ酸の製造方法
JP2010183860A (ja) 2009-02-11 2010-08-26 Shimane Univ 常温型アスパラギン酸脱水素酵素およびｌ−アスパラギン酸製造方法
JP2013516958A (ja) 2010-01-15 2013-05-16 味の素株式会社 Ｌ−アスパラギン酸又はｌ−アスパラギン酸より誘導される代謝産物を生産する腸内細菌科の細菌、及びｌ−アスパラギン酸又はｌ−アスパラギン酸より誘導される代謝産物の製造方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3680324B2 (ja) 1993-08-24 2005-08-10 味の素株式会社 変異型ホスホエノールピルビン酸カルボキシラーゼとその遺伝子及びアミノ酸の製造方法
DE19959650A1 (de) * 1999-12-10 2001-06-13 Degussa Neue für die Gene sdhA, sdhB und sdhC codierende Nukleotidsequenzen
BRPI0417773A (pt) * 2003-12-18 2007-03-20 Basf Ag métodos para aumentar o fluxo metabólico através da via de pentose fosfato em um microorganismo e para produzir uma substáncia quìmica fina, e, microorganismo recombinante
JP4720114B2 (ja) * 2004-05-20 2011-07-13 三菱化学株式会社 オキザロ酢酸またはオキザロ酢酸誘導体の製造方法
CN101023178A (zh) 2004-09-17 2007-08-22 莱斯大学 高琥珀酸产出细菌
JP4647391B2 (ja) 2005-05-20 2011-03-09 財団法人地球環境産業技術研究機構 コリネ型細菌による高効率なジカルボン酸の製造方法
EP2495317A1 (en) * 2011-03-01 2012-09-05 Technische Universität Hamburg-Harburg Modified phosphoenolpyruvate carboxylase from Corynebacterium glutamicum and uses thereof
CN103981203B (zh) 2013-02-07 2018-01-12 中国科学院天津工业生物技术研究所 5‑氨基乙酰丙酸高产菌株及其制备方法和应用
KR101500073B1 (ko) 2013-04-23 2015-03-06 씨제이제일제당 (주) L-아르기닌 생산능이 향상된 코리네박테리움속 미생물 및 이를 이용한 l-아르기닌의 생산 방법
JP6933954B2 (ja) 2017-10-26 2021-09-08 ニューウェルブランズ・ジャパン合同会社 子守帯

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0570860B2 (https=) 1985-11-08 1993-10-06 Nippon Electric Co
JP2003503064A (ja) 1999-06-29 2003-01-28 アーカー−ダニエルズ−ミッドランド カンパニー 炭素同化の調節
JP2006254730A (ja) 2005-03-15 2006-09-28 Univ Of Tokushima 新規なアスパラギン酸デヒドロゲナーゼ
JP2006254795A (ja) 2005-03-17 2006-09-28 Shimane Univ アスパラギン酸脱水素酵素、アラニン脱水素酵素、ｌ−アスパラギン酸製造方法、および、ｄ−リンゴ酸製造方法
JP2007043947A (ja) 2005-08-09 2007-02-22 Research Institute Of Innovative Technology For The Earth コリネ型細菌を用いる還元条件でのアミノ酸の製造方法
JP2010183860A (ja) 2009-02-11 2010-08-26 Shimane Univ 常温型アスパラギン酸脱水素酵素およびｌ−アスパラギン酸製造方法
JP2013516958A (ja) 2010-01-15 2013-05-16 味の素株式会社 Ｌ−アスパラギン酸又はｌ−アスパラギン酸より誘導される代謝産物を生産する腸内細菌科の細菌、及びｌ−アスパラギン酸又はｌ−アスパラギン酸より誘導される代謝産物の製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
비특허문헌 1 : Litsanov B, Kabus A, Brocker M, Bott M. Microb Biotechnol. 2012 Jan; 5(1) : 116-28.
비특허문헌 2 : Chen Z, Bommareddy RR, Frank D, Rappert S, Zeng AP. Appl Environ Microbiol. 2014 Feb; 80(4) : 1388-93.
비특허문헌 3 : Wada M, Sawada K, Ogura K, Shimono Y, Hagiwara T, Sugimoto M, Onuki A, Yokota A. J Biosci Bioeng. 2016 Feb; 121(2) : 172-7.
비특허문헌 4 : Yano M1, Izui K. Eur J Biochem. 1997 Jul 1; 247(1) : 74-81.

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