KR20120099258A - 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 - Google Patents
화합물들의 효과적인 생산을 위한 미생물 엔지니어링 Download PDFInfo
- Publication number
- KR20120099258A KR20120099258A KR20127015612A KR20127015612A KR20120099258A KR 20120099258 A KR20120099258 A KR 20120099258A KR 20127015612 A KR20127015612 A KR 20127015612A KR 20127015612 A KR20127015612 A KR 20127015612A KR 20120099258 A KR20120099258 A KR 20120099258A
- Authority
- KR
- South Korea
- Prior art keywords
- gene
- mutations
- strain
- production
- succinic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 136
- 239000000126 substance Substances 0.000 title description 9
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 256
- 230000035772 mutation Effects 0.000 claims abstract description 162
- 239000001384 succinic acid Substances 0.000 claims abstract description 122
- 244000005700 microbiome Species 0.000 claims abstract description 110
- 150000001875 compounds Chemical class 0.000 claims abstract description 90
- 238000000034 method Methods 0.000 claims abstract description 88
- 230000002503 metabolic effect Effects 0.000 claims abstract description 50
- 150000007524 organic acids Chemical class 0.000 claims abstract description 24
- 235000005985 organic acids Nutrition 0.000 claims abstract description 18
- 230000001965 increasing effect Effects 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 170
- 230000012010 growth Effects 0.000 claims description 67
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 66
- 229910052799 carbon Inorganic materials 0.000 claims description 65
- 238000012217 deletion Methods 0.000 claims description 54
- 230000037430 deletion Effects 0.000 claims description 54
- 108090000790 Enzymes Proteins 0.000 claims description 53
- 102000004190 Enzymes Human genes 0.000 claims description 52
- 230000000694 effects Effects 0.000 claims description 48
- 101150015622 pyk gene Proteins 0.000 claims description 40
- 230000037361 pathway Effects 0.000 claims description 39
- 235000000346 sugar Nutrition 0.000 claims description 35
- 238000000855 fermentation Methods 0.000 claims description 34
- 230000004151 fermentation Effects 0.000 claims description 34
- 108091000080 Phosphotransferase Proteins 0.000 claims description 32
- 102000020233 phosphotransferase Human genes 0.000 claims description 32
- 101150100525 pykA gene Proteins 0.000 claims description 32
- 108020005115 Pyruvate Kinase Proteins 0.000 claims description 25
- 229930029653 phosphoenolpyruvate Natural products 0.000 claims description 25
- 230000004102 tricarboxylic acid cycle Effects 0.000 claims description 24
- 102000013009 Pyruvate Kinase Human genes 0.000 claims description 23
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 claims description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 claims description 18
- 101150037064 rpoA gene Proteins 0.000 claims description 18
- 101150042391 rpoC gene Proteins 0.000 claims description 17
- 101150053304 pykF gene Proteins 0.000 claims description 16
- 108010078791 Carrier Proteins Proteins 0.000 claims description 15
- FJEKYHHLGZLYAT-FKUIBCNASA-N galp Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1N=CNC=1)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(O)=O)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)CNC(=O)[C@H](CCCNC(N)=N)NC(=O)CNC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](C)N)[C@@H](C)O)C(C)C)C1=CNC=N1 FJEKYHHLGZLYAT-FKUIBCNASA-N 0.000 claims description 15
- 101150033931 gldA gene Proteins 0.000 claims description 14
- 230000001105 regulatory effect Effects 0.000 claims description 14
- 101150005487 ftsI gene Proteins 0.000 claims description 12
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 10
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 10
- 101100145480 Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) rpoC2 gene Proteins 0.000 claims description 10
- 239000013611 chromosomal DNA Substances 0.000 claims description 10
- 101150109946 rpo1C gene Proteins 0.000 claims description 10
- 101150103066 rpoC1 gene Proteins 0.000 claims description 10
- 101100038183 Dictyostelium discoideum polr2a gene Proteins 0.000 claims description 9
- 230000001419 dependent effect Effects 0.000 claims description 9
- 101150047139 rpo1N gene Proteins 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 7
- 101150032129 egsA gene Proteins 0.000 claims description 6
- 108010090279 galactose permease Proteins 0.000 claims description 6
- 238000012239 gene modification Methods 0.000 claims description 6
- 230000005017 genetic modification Effects 0.000 claims description 6
- 235000013617 genetically modified food Nutrition 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- 101150060462 pbpB gene Proteins 0.000 claims description 6
- 101150026570 ydcC gene Proteins 0.000 claims description 5
- 101150079492 ydcF gene Proteins 0.000 claims description 5
- 108010015895 Glycerone kinase Proteins 0.000 claims description 4
- 101150093502 galR gene Proteins 0.000 claims description 4
- 239000003112 inhibitor Substances 0.000 claims description 4
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 2
- 230000003828 downregulation Effects 0.000 claims description 2
- 150000004727 oxaloacetic acid derivatives Chemical class 0.000 claims description 2
- 101100389345 Bacillus subtilis (strain 168) ndoA gene Proteins 0.000 claims 2
- 101100244826 Escherichia coli (strain K12) pptA gene Proteins 0.000 claims 2
- 101150084760 ydcD gene Proteins 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 230000000116 mitigating effect Effects 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 14
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 abstract description 12
- 238000012824 chemical production Methods 0.000 abstract description 8
- 230000006872 improvement Effects 0.000 abstract description 5
- 230000010429 evolutionary process Effects 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 84
- 241000588724 Escherichia coli Species 0.000 description 78
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 65
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 48
- 239000008103 glucose Substances 0.000 description 48
- 210000004027 cell Anatomy 0.000 description 45
- 230000014509 gene expression Effects 0.000 description 36
- 102000004169 proteins and genes Human genes 0.000 description 35
- 235000018102 proteins Nutrition 0.000 description 34
- 230000008569 process Effects 0.000 description 33
- 101150091561 galP gene Proteins 0.000 description 29
- 108700028369 Alleles Proteins 0.000 description 28
- 230000002068 genetic effect Effects 0.000 description 26
- 101150088738 pckA gene Proteins 0.000 description 26
- 101150067708 pckG gene Proteins 0.000 description 26
- 101100310802 Dictyostelium discoideum splA gene Proteins 0.000 description 25
- 239000002609 medium Substances 0.000 description 25
- 230000000813 microbial effect Effects 0.000 description 21
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 20
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 19
- 230000015572 biosynthetic process Effects 0.000 description 19
- 229940076788 pyruvate Drugs 0.000 description 19
- 101150040476 galS gene Proteins 0.000 description 17
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 17
- 241000894006 Bacteria Species 0.000 description 16
- 241000644323 Escherichia coli C Species 0.000 description 16
- 230000037433 frameshift Effects 0.000 description 16
- 238000010353 genetic engineering Methods 0.000 description 16
- 239000000543 intermediate Substances 0.000 description 16
- 101100447620 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) galR gene Proteins 0.000 description 15
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 15
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 15
- 229940049920 malate Drugs 0.000 description 14
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 14
- 230000002829 reductive effect Effects 0.000 description 14
- -1 hexarose sugars Chemical class 0.000 description 13
- 150000008163 sugars Chemical class 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 12
- 101150058595 MDH gene Proteins 0.000 description 12
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 11
- 230000006870 function Effects 0.000 description 11
- 238000003780 insertion Methods 0.000 description 11
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 11
- 150000003839 salts Chemical class 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 230000032258 transport Effects 0.000 description 11
- 108700026244 Open Reading Frames Proteins 0.000 description 10
- 230000037431 insertion Effects 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 108020004707 nucleic acids Proteins 0.000 description 10
- 150000007523 nucleic acids Chemical class 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 10
- 150000001413 amino acids Chemical class 0.000 description 9
- 238000013459 approach Methods 0.000 description 9
- 229940009098 aspartate Drugs 0.000 description 9
- 239000011942 biocatalyst Substances 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 101150021516 dhaM gene Proteins 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 8
- 101100299477 Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) phbI gene Proteins 0.000 description 8
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 8
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 8
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 8
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 239000002253 acid Substances 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 8
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 8
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 8
- 230000004190 glucose uptake Effects 0.000 description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 description 8
- 230000037353 metabolic pathway Effects 0.000 description 8
- 235000010755 mineral Nutrition 0.000 description 8
- 239000011707 mineral Substances 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 101150118630 ptsI gene Proteins 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 7
- 241000186146 Brevibacterium Species 0.000 description 7
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 7
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 7
- 239000004472 Lysine Substances 0.000 description 7
- 101100381593 Planococcus sp. (strain L4) bgaP gene Proteins 0.000 description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 7
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 7
- 239000004473 Threonine Substances 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 231100000221 frame shift mutation induction Toxicity 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 229930027917 kanamycin Natural products 0.000 description 7
- 229960000318 kanamycin Drugs 0.000 description 7
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 7
- 229930182823 kanamycin A Natural products 0.000 description 7
- 235000018977 lysine Nutrition 0.000 description 7
- 229960003646 lysine Drugs 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 229960002898 threonine Drugs 0.000 description 7
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 241000590020 Achromobacter Species 0.000 description 6
- 241000588722 Escherichia Species 0.000 description 6
- 101100182965 Escherichia coli (strain K12) maeA gene Proteins 0.000 description 6
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 6
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 230000009471 action Effects 0.000 description 6
- 230000009604 anaerobic growth Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 229930182830 galactose Natural products 0.000 description 6
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 6
- 239000001630 malic acid Substances 0.000 description 6
- 235000011090 malic acid Nutrition 0.000 description 6
- 229940099690 malic acid Drugs 0.000 description 6
- 229930182817 methionine Natural products 0.000 description 6
- 229960004452 methionine Drugs 0.000 description 6
- 230000001590 oxidative effect Effects 0.000 description 6
- 101150111581 pflB gene Proteins 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 5
- 241000589565 Flavobacterium Species 0.000 description 5
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 5
- 101150093629 PYK1 gene Proteins 0.000 description 5
- 102000003673 Symporters Human genes 0.000 description 5
- 108090000088 Symporters Proteins 0.000 description 5
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 5
- 101150006213 ackA gene Proteins 0.000 description 5
- 101150014383 adhE gene Proteins 0.000 description 5
- 230000009603 aerobic growth Effects 0.000 description 5
- 229940024606 amino acid Drugs 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 5
- 150000001720 carbohydrates Chemical class 0.000 description 5
- 229960005091 chloramphenicol Drugs 0.000 description 5
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 5
- 229940050411 fumarate Drugs 0.000 description 5
- 239000001530 fumaric acid Substances 0.000 description 5
- 235000011087 fumaric acid Nutrition 0.000 description 5
- 238000012224 gene deletion Methods 0.000 description 5
- 239000006481 glucose medium Substances 0.000 description 5
- 230000034659 glycolysis Effects 0.000 description 5
- ODBLHEXUDAPZAU-UHFFFAOYSA-N isocitric acid Chemical compound OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 5
- 230000026731 phosphorylation Effects 0.000 description 5
- 238000006366 phosphorylation reaction Methods 0.000 description 5
- 230000002441 reversible effect Effects 0.000 description 5
- 101150025220 sacB gene Proteins 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 229940086735 succinate Drugs 0.000 description 5
- 238000010361 transduction Methods 0.000 description 5
- 230000026683 transduction Effects 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- JOOXCMJARBKPKM-UHFFFAOYSA-N 4-oxopentanoic acid Chemical compound CC(=O)CCC(O)=O JOOXCMJARBKPKM-UHFFFAOYSA-N 0.000 description 4
- 101100456369 Aquifex aeolicus (strain VF5) mdh1 gene Proteins 0.000 description 4
- 101150085381 CDC19 gene Proteins 0.000 description 4
- 206010064571 Gene mutation Diseases 0.000 description 4
- 108020003285 Isocitrate lyase Proteins 0.000 description 4
- 241000588748 Klebsiella Species 0.000 description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 4
- 102000015841 Major facilitator superfamily Human genes 0.000 description 4
- 108050004064 Major facilitator superfamily Proteins 0.000 description 4
- 101100384788 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) comC gene Proteins 0.000 description 4
- 101100020705 Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) ldh gene Proteins 0.000 description 4
- 101100234604 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ace-8 gene Proteins 0.000 description 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 4
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 4
- 101100290490 Rattus norvegicus Mdh1 gene Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- 230000008238 biochemical pathway Effects 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 4
- 230000009567 fermentative growth Effects 0.000 description 4
- CHTHALBTIRVDBM-UHFFFAOYSA-N furan-2,5-dicarboxylic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)O1 CHTHALBTIRVDBM-UHFFFAOYSA-N 0.000 description 4
- 102000034356 gene-regulatory proteins Human genes 0.000 description 4
- 108091006104 gene-regulatory proteins Proteins 0.000 description 4
- 229930195712 glutamate Natural products 0.000 description 4
- 229940049906 glutamate Drugs 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 101150067967 iclR gene Proteins 0.000 description 4
- 230000000415 inactivating effect Effects 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 101150083023 mgsA gene Proteins 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 150000002972 pentoses Chemical class 0.000 description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 4
- 101150096049 pyc gene Proteins 0.000 description 4
- 229940107700 pyruvic acid Drugs 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000002269 spontaneous effect Effects 0.000 description 4
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 4
- 229960003495 thiamine Drugs 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 230000003827 upregulation Effects 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 101100490184 Drosophila melanogaster Ack gene Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 3
- 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 3
- 241000187561 Rhodococcus erythropolis Species 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 102000037089 Uniporters Human genes 0.000 description 3
- 108091006293 Uniporters Proteins 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 101150005925 aspC gene Proteins 0.000 description 3
- 235000003704 aspartic acid Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 3
- 229960003237 betaine Drugs 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 230000008827 biological function Effects 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 150000001722 carbon compounds Chemical class 0.000 description 3
- 230000006860 carbon metabolism Effects 0.000 description 3
- 238000006473 carboxylation reaction Methods 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 3
- 101150020338 dnaA gene Proteins 0.000 description 3
- 238000009510 drug design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 150000002402 hexoses Chemical class 0.000 description 3
- 238000002744 homologous recombination Methods 0.000 description 3
- 230000006801 homologous recombination Effects 0.000 description 3
- 230000004941 influx Effects 0.000 description 3
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
- 229960000310 isoleucine Drugs 0.000 description 3
- 101150108859 maeB gene Proteins 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000010627 oxidative phosphorylation Effects 0.000 description 3
- 101150060030 poxB gene Proteins 0.000 description 3
- 101150045242 ptsH gene Proteins 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 101150019205 tdcD gene Proteins 0.000 description 3
- 239000012137 tryptone Substances 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 2
- ALRHLSYJTWAHJZ-UHFFFAOYSA-N 3-hydroxypropionic acid Chemical compound OCCC(O)=O ALRHLSYJTWAHJZ-UHFFFAOYSA-N 0.000 description 2
- 230000002407 ATP formation Effects 0.000 description 2
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 2
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 2
- 241000606750 Actinobacillus Species 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 101100233177 Bacillus subtilis (strain 168) int gene Proteins 0.000 description 2
- 244000177578 Bacterium linens Species 0.000 description 2
- 235000012539 Bacterium linens Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 101100499417 Chlamydia pneumoniae dnaA1 gene Proteins 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 108010071536 Citrate (Si)-synthase Proteins 0.000 description 2
- 108010036824 Citrate (pro-3S)-lyase Proteins 0.000 description 2
- 102000006732 Citrate synthase Human genes 0.000 description 2
- 241000186216 Corynebacterium Species 0.000 description 2
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- 101100138542 Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) phbH gene Proteins 0.000 description 2
- 101710154303 Cyclic AMP receptor protein Proteins 0.000 description 2
- 229930182843 D-Lactic acid Natural products 0.000 description 2
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 2
- 241000199914 Dinophyceae Species 0.000 description 2
- 241000588698 Erwinia Species 0.000 description 2
- 101100139916 Escherichia coli (strain K12) rarA gene Proteins 0.000 description 2
- 241000191070 Escherichia coli ATCC 8739 Species 0.000 description 2
- 241000901842 Escherichia coli W Species 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 101000878540 Homo sapiens Protein-tyrosine kinase 2-beta Proteins 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- 101100433987 Latilactobacillus sakei subsp. sakei (strain 23K) ackA1 gene Proteins 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 241001293415 Mannheimia Species 0.000 description 2
- AIJULSRZWUXGPQ-UHFFFAOYSA-N Methylglyoxal Chemical compound CC(=O)C=O AIJULSRZWUXGPQ-UHFFFAOYSA-N 0.000 description 2
- 241000191936 Micrococcus sp. Species 0.000 description 2
- 101100353526 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pca-2 gene Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 241000364057 Peoria Species 0.000 description 2
- 101100462488 Phlebiopsis gigantea p2ox gene Proteins 0.000 description 2
- 108090000472 Phosphoenolpyruvate carboxykinase (ATP) Proteins 0.000 description 2
- 102100037787 Protein-tyrosine kinase 2-beta Human genes 0.000 description 2
- 108010091086 Recombinases Proteins 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 2
- 108700005075 Regulator Genes Proteins 0.000 description 2
- 241000187562 Rhodococcus sp. Species 0.000 description 2
- 241000235070 Saccharomyces Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000607720 Serratia Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 241000218589 Streptomyces olivaceus Species 0.000 description 2
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 2
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 2
- 101100492609 Talaromyces wortmannii astC gene Proteins 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- 101150116772 aatA gene Proteins 0.000 description 2
- 235000011054 acetic acid Nutrition 0.000 description 2
- 230000002730 additional effect Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 230000021523 carboxylation Effects 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 101150000622 csrA gene Proteins 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 229940022769 d- lactic acid Drugs 0.000 description 2
- 101150100742 dapL gene Proteins 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 229940120503 dihydroxyacetone Drugs 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 108010008221 formate C-acetyltransferase Proteins 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 238000011331 genomic analysis Methods 0.000 description 2
- 230000004110 gluconeogenesis Effects 0.000 description 2
- 230000001890 gluconeogenic effect Effects 0.000 description 2
- 230000006377 glucose transport Effects 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 229940040102 levulinic acid Drugs 0.000 description 2
- 101150052159 maeA gene Proteins 0.000 description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000006151 minimal media Substances 0.000 description 2
- 101150068440 msrB gene Proteins 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 108010071189 phosphoenolpyruvate-glucose phosphotransferase Proteins 0.000 description 2
- LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 2
- 235000015497 potassium bicarbonate Nutrition 0.000 description 2
- 239000011736 potassium bicarbonate Substances 0.000 description 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 2
- 229910000027 potassium carbonate Inorganic materials 0.000 description 2
- 235000011181 potassium carbonates Nutrition 0.000 description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 2
- 101150023641 ppc gene Proteins 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 150000004728 pyruvic acid derivatives Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000004767 rumen Anatomy 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 235000019157 thiamine Nutrition 0.000 description 2
- 239000011721 thiamine Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 101150092141 ydcL gene Proteins 0.000 description 2
- 101150055770 ydcO gene Proteins 0.000 description 2
- 101150004008 ydcV gene Proteins 0.000 description 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- BSABBBMNWQWLLU-GSVOUGTGSA-N (R)-lactaldehyde Chemical compound C[C@@H](O)C=O BSABBBMNWQWLLU-GSVOUGTGSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UWTATZPHSA-M (R)-lactate Chemical compound C[C@@H](O)C([O-])=O JVTAAEKCZFNVCJ-UWTATZPHSA-M 0.000 description 1
- WIIZWVCIJKGZOK-IUCAKERBSA-N 2,2-dichloro-n-[(1s,2s)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide Chemical compound ClC(Cl)C(=O)N[C@@H](CO)[C@@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-IUCAKERBSA-N 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- FUDDLSHBRSNCBV-UHFFFAOYSA-N 4-hydroxyoxolan-2-one Chemical compound OC1COC(=O)C1 FUDDLSHBRSNCBV-UHFFFAOYSA-N 0.000 description 1
- 108010092060 Acetate kinase Proteins 0.000 description 1
- 102000008146 Acetate-CoA ligase Human genes 0.000 description 1
- 108010049926 Acetate-CoA ligase Proteins 0.000 description 1
- 108010009924 Aconitate hydratase Proteins 0.000 description 1
- 241000187254 Actinomadura madurae Species 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000607525 Aeromonas salmonicida Species 0.000 description 1
- 241000588813 Alcaligenes faecalis Species 0.000 description 1
- 101100163490 Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) aroA1 gene Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241001566735 Archon Species 0.000 description 1
- 241000185996 Arthrobacter citreus Species 0.000 description 1
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 101100130094 Bacillus subtilis (strain 168) metK gene Proteins 0.000 description 1
- 101100350224 Bacillus subtilis (strain 168) pdhB gene Proteins 0.000 description 1
- 241001430265 Beijerinckia indica subsp. indica Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000218649 Brevibacterium fuscum Species 0.000 description 1
- CIAORQUOMVZHNA-UHFFFAOYSA-N C(C(=O)C)(=O)O.P(=O)(=O)OC1=CC=CC=C1 Chemical compound C(C(=O)C)(=O)O.P(=O)(=O)OC1=CC=CC=C1 CIAORQUOMVZHNA-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 1
- 241000588919 Citrobacter freundii Species 0.000 description 1
- 241000589518 Comamonas testosteroni Species 0.000 description 1
- 241001517047 Corynebacterium acetoacidophilum Species 0.000 description 1
- 241000186145 Corynebacterium ammoniagenes Species 0.000 description 1
- 101100236536 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) glcB gene Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000983382 Curtobacterium pusillum Species 0.000 description 1
- 102100039868 Cytoplasmic aconitate hydratase Human genes 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- HEBKCHPVOIAQTA-QWWZWVQMSA-N D-arabinitol Chemical compound OC[C@@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-QWWZWVQMSA-N 0.000 description 1
- DSLZVSRJTYRBFB-LLEIAEIESA-N D-glucaric acid Chemical compound OC(=O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O DSLZVSRJTYRBFB-LLEIAEIESA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 102100030960 DNA replication licensing factor MCM2 Human genes 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 241000588700 Dickeya chrysanthemi Species 0.000 description 1
- 101100378193 Dictyostelium discoideum aco2 gene Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000589566 Elizabethkingia meningoseptica Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 101100497471 Escherichia coli (strain K12) csrD gene Proteins 0.000 description 1
- 101100174518 Escherichia coli (strain K12) fumB gene Proteins 0.000 description 1
- 101100099023 Escherichia coli (strain K12) tdcE gene Proteins 0.000 description 1
- 241001522878 Escherichia coli B Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000702191 Escherichia virus P1 Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241001518248 Gluconobacter cerinus Species 0.000 description 1
- 108091006065 Gs proteins Proteins 0.000 description 1
- 101000583807 Homo sapiens DNA replication licensing factor MCM2 Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 241000204057 Kitasatospora Species 0.000 description 1
- 241000588915 Klebsiella aerogenes Species 0.000 description 1
- 241000588749 Klebsiella oxytoca Species 0.000 description 1
- 102000000428 Lactate Dehydrogenases Human genes 0.000 description 1
- 108010080864 Lactate Dehydrogenases Proteins 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 241001625930 Luria Species 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- 108020004687 Malate Synthase Proteins 0.000 description 1
- 101710161955 Mannitol-specific phosphotransferase enzyme IIA component Proteins 0.000 description 1
- 241001467578 Microbacterium Species 0.000 description 1
- 241000144155 Microbacterium ammoniaphilum Species 0.000 description 1
- 241000983412 Microbacterium saperdae Species 0.000 description 1
- 241000203815 Microbacterium testaceum Species 0.000 description 1
- 241000588772 Morganella morganii Species 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 101150031320 PYK2 gene Proteins 0.000 description 1
- 241000227676 Paenibacillus thiaminolyticus Species 0.000 description 1
- 241000588912 Pantoea agglomerans Species 0.000 description 1
- 241000588701 Pectobacterium carotovorum Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 101100192216 Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) ptaA gene Proteins 0.000 description 1
- 241000193804 Planococcus <bacterium> Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000157304 Prauserella rugosa Species 0.000 description 1
- 108090001084 Propionate kinases Proteins 0.000 description 1
- 241000186334 Propionibacterium freudenreichii subsp. shermanii Species 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 241000588768 Providencia Species 0.000 description 1
- 241000588777 Providencia rettgeri Species 0.000 description 1
- 241000185994 Pseudarthrobacter oxydans Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 101100406344 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceF gene Proteins 0.000 description 1
- 241000218935 Pseudomonas azotoformans Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000204709 Pseudomonas mucidolens Species 0.000 description 1
- 241000589614 Pseudomonas stutzeri Species 0.000 description 1
- 241000218902 Pseudomonas synxantha Species 0.000 description 1
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 description 1
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 1
- 108010032060 RNA polymerase alpha subunit Proteins 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241001524101 Rhodococcus opacus Species 0.000 description 1
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 241000186652 Sporosarcina ureae Species 0.000 description 1
- 101100398785 Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) ldhD gene Proteins 0.000 description 1
- 241000186988 Streptomyces antibioticus Species 0.000 description 1
- 241000186990 Streptomyces cacaoi Species 0.000 description 1
- 241000187435 Streptomyces griseolus Species 0.000 description 1
- 241000187389 Streptomyces lavendulae Species 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- 241000946755 Streptomyces tanashiensis Species 0.000 description 1
- 241000946734 Streptomyces violaceochromogenes Species 0.000 description 1
- 241000187122 Streptomyces virginiae Species 0.000 description 1
- 241000187191 Streptomyces viridochromogenes Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 108700025695 Suppressor Genes Proteins 0.000 description 1
- 241000866060 Terrabacter tumescens Species 0.000 description 1
- 101100466011 Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) pta gene Proteins 0.000 description 1
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 241001135144 Vibrio metschnikovii Species 0.000 description 1
- 241000589655 Xanthomonas citri Species 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 101100386830 Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) ddh gene Proteins 0.000 description 1
- 241000319304 [Brevibacterium] flavum Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 101150036393 aceB gene Proteins 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- 101150053555 acnB gene Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229940005347 alcaligenes faecalis Drugs 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000037354 amino acid metabolism Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 101150075954 apeB gene Proteins 0.000 description 1
- SCJNCDSAIRBRIA-DOFZRALJSA-N arachidonyl-2'-chloroethylamide Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)NCCCl SCJNCDSAIRBRIA-DOFZRALJSA-N 0.000 description 1
- 101150037081 aroA gene Proteins 0.000 description 1
- 101150040872 aroE gene Proteins 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 235000020299 breve Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000009015 carbon catabolite repression of transcription Effects 0.000 description 1
- 150000007942 carboxylates Chemical class 0.000 description 1
- HOPSCVCBEOCPJZ-UHFFFAOYSA-N carboxymethyl(trimethyl)azanium;chloride Chemical compound [Cl-].C[N+](C)(C)CC(O)=O HOPSCVCBEOCPJZ-UHFFFAOYSA-N 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 description 1
- 101150028648 citZ gene Proteins 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 125000004093 cyano group Chemical group *C#N 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000459 effect on growth Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 229940092559 enterobacter aerogenes Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 101150077334 focA gene Proteins 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 229960002598 fumaric acid Drugs 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000030414 genetic transfer Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000012405 in silico analysis Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 1
- 101150001899 lacY gene Proteins 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 229940001447 lactate Drugs 0.000 description 1
- 101150026107 ldh1 gene Proteins 0.000 description 1
- 101150041530 ldha gene Proteins 0.000 description 1
- 101150087199 leuA gene Proteins 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000012976 mRNA stabilization Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 101150108178 metE gene Proteins 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 108010083856 methylglyoxal synthase Proteins 0.000 description 1
- 229940054192 micro-guard Drugs 0.000 description 1
- 238000010208 microarray analysis Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000007003 mineral medium Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
- 229940076266 morganella morganii Drugs 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 150000004719 oxaloacetic acids Chemical class 0.000 description 1
- 101150094986 pepC gene Proteins 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 101150004519 pilC gene Proteins 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000020004 porter Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 101150108780 pta gene Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000009331 reductive pathway Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 101150040968 rhaS gene Proteins 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 150000003890 succinate salts Chemical class 0.000 description 1
- VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 101150062749 uvrY gene Proteins 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000001238 wet grinding Methods 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 101150042423 ydcI gene Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B6/00—Apparatus or devices for radiation diagnosis; Apparatus or devices for radiation diagnosis combined with radiation therapy equipment
- A61B6/04—Positioning of patients; Tiltable beds or the like
- A61B6/0407—Supports, e.g. tables or beds, for the body or parts of the body
- A61B6/0442—Supports, e.g. tables or beds, for the body or parts of the body made of non-metallic materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C70/00—Shaping composites, i.e. plastics material comprising reinforcements, fillers or preformed parts, e.g. inserts
- B29C70/04—Shaping composites, i.e. plastics material comprising reinforcements, fillers or preformed parts, e.g. inserts comprising reinforcements only, e.g. self-reinforcing plastics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- B29L2031/00—Other particular articles
- B29L2031/753—Medical equipment; Accessories therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medical Informatics (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Heart & Thoracic Surgery (AREA)
- Mechanical Engineering (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Composite Materials (AREA)
- Radiology & Medical Imaging (AREA)
- High Energy & Nuclear Physics (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28148109P | 2009-11-18 | 2009-11-18 | |
| US61/281,481 | 2009-11-18 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020167025628A Division KR20160114184A (ko) | 2009-11-18 | 2010-11-17 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20120099258A true KR20120099258A (ko) | 2012-09-07 |
Family
ID=44060323
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20127015612A Ceased KR20120099258A (ko) | 2009-11-18 | 2010-11-17 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
| KR1020167025628A Ceased KR20160114184A (ko) | 2009-11-18 | 2010-11-17 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020167025628A Ceased KR20160114184A (ko) | 2009-11-18 | 2010-11-17 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9017976B2 (enExample) |
| EP (1) | EP2501797A4 (enExample) |
| JP (1) | JP2013511277A (enExample) |
| KR (2) | KR20120099258A (enExample) |
| CN (1) | CN102666838B (enExample) |
| BR (1) | BR112012011990A2 (enExample) |
| WO (1) | WO2011063055A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9758771B2 (en) | 2013-06-25 | 2017-09-12 | Cj Cheiljedang Corporation | Microorganism with enhanced L-lysine productivity and method for producing L-lysine by using same |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120103645A (ko) * | 2009-11-18 | 2012-09-19 | 미리안트 코포레이션 | 유기산들을 생산하는 대장균의 대사적 진화 |
| WO2012019175A2 (en) | 2010-08-06 | 2012-02-09 | Mascoma Corporation | Production of malonyl-coa drived products via anaerobic pathways |
| JP2015504688A (ja) * | 2012-01-30 | 2015-02-16 | ミリアント・コーポレイションMyriant Corporation | 遺伝子操作された微生物からのムコン酸の生成 |
| CN102994436A (zh) * | 2012-10-29 | 2013-03-27 | 江南大学 | 一种产减毒类脂a的大肠杆菌基因工程菌及其应用 |
| JP6085190B2 (ja) * | 2013-02-26 | 2017-02-22 | 花王株式会社 | 変異微生物及びそれを用いた有用物質の生産方法 |
| CN105452445B (zh) * | 2013-07-23 | 2020-08-21 | Ptt全球化学公众有限公司 | 利用用于糖输入的易化扩散生产琥珀酸和其它化学品的方法 |
| EP3041942B1 (en) | 2013-09-03 | 2020-07-01 | PTT Global Chemical Public Company Limited | A process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol |
| CN104046586B (zh) * | 2014-07-02 | 2016-08-17 | 山东大学 | 一株基因工程菌及其在生产(2r,3r)-2,3-丁二醇中的应用 |
| CN107208118A (zh) * | 2014-09-18 | 2017-09-26 | 基因组股份公司 | 具有提高的能源效率的非天然微生物 |
| US10301613B2 (en) | 2015-09-15 | 2019-05-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Targeted remodeling of prokaryotic genomes using CRISPR-nickases |
| EP3423563B1 (en) | 2016-03-02 | 2023-05-24 | PTT Global Chemical Public Company Limited | Improved muconic acid production from genetically engineered microorganisms |
| MD3448859T2 (ro) | 2017-03-20 | 2020-03-31 | Forma Therapeutics Inc | Compoziții de pirolopirol în calitate de activatori ai piruvatkinazei (PKR) |
| KR102683624B1 (ko) | 2017-06-30 | 2024-07-11 | 피티티 글로벌 케미칼 피씨엘 | 기능적 dna 서열의 안정화된 카피 수를 갖는 미생물 및 관련 방법 |
| WO2020061378A1 (en) | 2018-09-19 | 2020-03-26 | Forma Therapeutics, Inc. | Treating sickle cell disease with a pyruvate kinase r activating compound |
| ES2989438T3 (es) | 2018-09-19 | 2024-11-26 | Novo Nordisk Healthcare Ag | Activación de la piruvato cinasa R |
| CN109251941B (zh) * | 2018-09-30 | 2020-11-06 | 江南大学 | 一种高产琥珀酸的大肠杆菌及其应用 |
| KR102129379B1 (ko) * | 2018-10-10 | 2020-07-02 | 한국과학기술원 | 고활성의 말산 탈수소효소가 도입된 숙신산 생성용 변이 미생물 및 이를 이용한 숙신산 제조방법 |
| AU2020350763A1 (en) | 2019-09-19 | 2022-04-07 | Novo Nordisk Health Care Ag | Pyruvate kinase R (PKR) activating compositions |
| CN113278641B (zh) * | 2020-05-27 | 2022-06-21 | 安徽华恒生物科技股份有限公司 | 生产l-缬氨酸的重组大肠杆菌、其构建方法及其应用 |
| IT202000013243A1 (it) | 2020-06-04 | 2021-12-04 | Novamont Spa | Processo per la purificazione di una miscela di dioli |
| IT202000031979A1 (it) | 2020-12-22 | 2022-06-22 | Novamont Spa | Processo di purificazione di una miscela comprendente dioli e acetali |
| IT202100006419A1 (it) | 2021-03-17 | 2022-09-17 | Novamont Spa | Recupero di dioli da una miscela |
| US12128035B2 (en) | 2021-03-19 | 2024-10-29 | Novo Nordisk Health Care Ag | Activating pyruvate kinase R |
| IT202100030572A1 (it) | 2021-12-02 | 2023-06-02 | Novamont Spa | 1,3-butandiolo purificato da una miscela di dioli |
| CN118546852B (zh) * | 2024-07-30 | 2024-10-18 | 北京化工大学 | 一种高效共利用葡萄糖、木糖生产丁二酸的基因工程菌及其应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1947186B1 (en) | 1995-05-05 | 2011-11-16 | Danisco US Inc. | Application of glucose transport mutants for production of aromatic pathway compounds |
| MY121380A (en) | 1998-04-13 | 2006-01-28 | Univ Georgia | Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells |
| US6989265B2 (en) | 2002-01-23 | 2006-01-24 | Wisconsin Alumni Research Foundation | Bacteria with reduced genome |
| US7303906B2 (en) | 2002-09-06 | 2007-12-04 | Wisconsin Alumni Research Foundation | Competent bacteria |
| KR101148255B1 (ko) | 2002-10-04 | 2012-08-08 | 다니스코 유에스 인크. | 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법 |
| US7232665B2 (en) | 2002-12-19 | 2007-06-19 | E. I. Du Pont De Nemours And Company | Mutations affecting carotenoid production |
| DE10314618A1 (de) | 2003-04-01 | 2004-10-14 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
| CN103555782B (zh) | 2003-10-21 | 2017-04-12 | 嘉吉有限公司 | 生产莫纳甜和莫纳甜前体 |
| KR100576342B1 (ko) | 2004-02-05 | 2006-05-03 | 씨제이 주식회사 | galR 유전자가 불활성화된 L-쓰레오닌 생성 미생물,그를 제조하는 방법 및 상기 미생물을 이용한L-쓰레오닌의 제조방법 |
| US20050181488A1 (en) | 2004-02-12 | 2005-08-18 | Akhverdian Valery Z. | Method for producing L-threonine using bacteria belonging to the genus Escherichia |
| KR101245428B1 (ko) | 2004-08-27 | 2013-03-19 | 라이스 유니버시티 | 숙신산 생산이 증가된 돌연변이 대장균 균주 |
| US20060073577A1 (en) | 2004-09-17 | 2006-04-06 | San Ka-Yiu | High succinate producing bacteria |
| US20080009041A1 (en) | 2004-11-26 | 2008-01-10 | Hiroshi Mizoguchi | Industrially Useful Microorganism |
| KR100630836B1 (ko) | 2005-04-08 | 2006-10-02 | 한국과학기술원 | 인-실리코 분석을 통한 균주 개량방법 |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| EP2305826B1 (en) | 2005-08-10 | 2016-03-30 | University of Florida Research Foundation, Inc. | Materials and methods for efficient lactic acid production |
| US8178339B2 (en) | 2005-08-20 | 2012-05-15 | Scarab Genomics Llc | Reduced genome E. coli |
| EP1937821A4 (en) | 2005-09-09 | 2009-11-11 | Genomatica Inc | PROCESS AND ORGANISMS FOR GROWTH-COUPLED PRODUCTION OF SUCCINATE |
| DE102005047596A1 (de) | 2005-10-05 | 2007-04-12 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| DK1944365T3 (da) | 2005-10-13 | 2012-04-30 | Kao Corp | Hidtil ukendt bacillus subtilis mutantstamme |
| KR101390085B1 (ko) * | 2006-03-31 | 2014-04-28 | 라이스 유니버시티 | 글리세롤의 혐기성 발효 |
| KR100885616B1 (ko) | 2006-06-26 | 2009-02-24 | 씨제이제일제당 (주) | 글리세롤을 이용한 아미노산의 생산 방법 |
| EP2570481B1 (en) | 2007-03-20 | 2014-11-26 | University of Florida Research Foundation, Inc. | Materials and methods for efficient succinate and malate production |
| JP2010521190A (ja) | 2007-03-23 | 2010-06-24 | メタボリック エクスプローラー | 1,2−プロパンジオールの製造に有用な、代謝的に操作された微生物 |
| US20100279369A1 (en) | 2007-03-23 | 2010-11-04 | Metabolic Explorer | Metabolically engineered microorganism useful for the production of acetol |
| US20110039327A1 (en) * | 2007-05-18 | 2011-02-17 | Aaron Adriaan Winkler | Organic acid production by fungal cells |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| WO2009043372A1 (en) | 2007-10-02 | 2009-04-09 | Metabolic Explorer | Increasing methionine yield |
| US20100330614A1 (en) * | 2007-11-06 | 2010-12-30 | Massachusetts Institute Of Technology | Global transcription machinery engineering targeting the rnap alpha subunit (rpoa) |
| EP2241630B1 (en) | 2007-12-06 | 2016-06-01 | Ajinomoto Co., Inc. | A method for producing an organic acid |
| CA2779262C (en) | 2008-10-31 | 2021-09-07 | Gevo, Inc. | Engineered microorganisms capable of converting pyruvate to isobutanol under anaerobic conditions |
| KR20120025450A (ko) | 2009-04-02 | 2012-03-15 | 유니버시티 오브 플로리다 리서치 파운데이션, 인크. | 석시네이트 생성을 위한 경로의 엔지니어링 |
-
2010
- 2010-11-17 WO PCT/US2010/057119 patent/WO2011063055A2/en not_active Ceased
- 2010-11-17 CN CN201080052398.3A patent/CN102666838B/zh active Active
- 2010-11-17 KR KR20127015612A patent/KR20120099258A/ko not_active Ceased
- 2010-11-17 KR KR1020167025628A patent/KR20160114184A/ko not_active Ceased
- 2010-11-17 BR BR112012011990A patent/BR112012011990A2/pt not_active Application Discontinuation
- 2010-11-17 US US13/503,999 patent/US9017976B2/en active Active
- 2010-11-17 EP EP10832141.5A patent/EP2501797A4/en not_active Withdrawn
- 2010-11-17 JP JP2012540031A patent/JP2013511277A/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9758771B2 (en) | 2013-06-25 | 2017-09-12 | Cj Cheiljedang Corporation | Microorganism with enhanced L-lysine productivity and method for producing L-lysine by using same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102666838B (zh) | 2018-04-10 |
| WO2011063055A2 (en) | 2011-05-26 |
| EP2501797A2 (en) | 2012-09-26 |
| EP2501797A4 (en) | 2013-04-17 |
| BR112012011990A2 (pt) | 2015-09-29 |
| WO2011063055A3 (en) | 2011-11-17 |
| US20120220000A1 (en) | 2012-08-30 |
| US9017976B2 (en) | 2015-04-28 |
| JP2013511277A (ja) | 2013-04-04 |
| CN102666838A (zh) | 2012-09-12 |
| KR20160114184A (ko) | 2016-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20120099258A (ko) | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 | |
| US10934565B2 (en) | Method of producing succinic acid and other chemicals using facilitated diffusion for sugar import | |
| KR101928688B1 (ko) | 유기산으로의 글리세롤의 발효 | |
| EP2665809B1 (en) | A microorganism having enhanced l-amino acids productivity and process for producing l-amino acids using the same | |
| JP2019195330A (ja) | 遺伝子操作された微生物からのムコン酸の生成 | |
| JP7437116B2 (ja) | 遺伝子操作された微生物からのムコン酸生成の改善 | |
| CN107614516A (zh) | 用于基因工程改造甲基营养型酵母的表达构建体和方法 | |
| ITMI972080A1 (it) | Ceppi di lievito per la riproduzione di acido lattico | |
| KR20160079897A (ko) | 유기산들을 생산하는 대장균의 대사적 진화 | |
| BRPI0809400A2 (pt) | Materiais e métodos para a produção eficiente de succinato e malato | |
| BR112014026720B1 (pt) | Cepa de lactobacillus sp. produtora de d-ácido lático, método de preparação da dita cepa e método para a produção de d-ácido lático | |
| EP0934418B1 (en) | Microbial preparation of substances from aromatic metabolism/i | |
| KR20200023451A (ko) | 숙신산 생성이 증가된 유전자 변형 효모 | |
| JPS62186795A (ja) | アミノ酸の製造法 | |
| KR101773123B1 (ko) | 2,3-부탄다이올 생산능을 갖는 유전적으로 조작된 효모 세포 및 그를 사용하여 2,3-부탄다이올을 생산하는 방법 | |
| KR101960382B1 (ko) | 호기조건에서 부탄올을 생성하는 변이 미생물 및 이를 이용한 부탄올의 제조방법 | |
| US20250250603A1 (en) | Method for preparing s-lactoylglutathione | |
| KR20230143916A (ko) | 신규한 시스 아코니트산 합성 효소 및 이의 용도 | |
| CN120829911A (zh) | 一种利用乙二醇合成l-异亮氨酸的大肠杆菌工程菌株及其应用 | |
| CN120944790A (zh) | L-异亮氨酸生产能力得到提高的埃希菌属微生物及利用其的l-异亮氨酸的生产方法 | |
| Mikola | Optimization of biocatalytic 3-dehydroshikimic acid production from D-glucose in Escherichia coli | |
| CN120158468A (zh) | 一株利用乙二醇提高l-赖氨酸产量的大肠杆菌工程菌株的构建方法及其应用 | |
| KR20220166605A (ko) | 갈락토스 이용에 관여하는 모든 유전자가 결손된 효모 균주 및 이를 이용한 재조합 단백질 생산방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20120615 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| A302 | Request for accelerated examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20151016 Comment text: Request for Examination of Application |
|
| PA0302 | Request for accelerated examination |
Patent event date: 20151016 Patent event code: PA03022R01D Comment text: Request for Accelerated Examination |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20151203 Patent event code: PE09021S01D |
|
| E601 | Decision to refuse application | ||
| PE0601 | Decision on rejection of patent |
Patent event date: 20160620 Comment text: Decision to Refuse Application Patent event code: PE06012S01D Patent event date: 20151203 Comment text: Notification of reason for refusal Patent event code: PE06011S01I |
|
| A107 | Divisional application of patent | ||
| PA0104 | Divisional application for international application |
Comment text: Divisional Application for International Patent Patent event code: PA01041R01D Patent event date: 20160919 |