JP2013511277A - 化学物質の効率的な生産のための微生物の改変 - Google Patents
化学物質の効率的な生産のための微生物の改変 Download PDFInfo
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2016528893A (ja) * | 2013-07-23 | 2016-09-23 | ミリアント・コーポレイションＭｙｒｉａｎｔ Ｃｏｒｐｏｒａｔｉｏｎ | 糖の取り込みのための促進拡散を用いるコハク酸および他の化合物の生産方法 |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102686718B (zh) * | 2009-11-18 | 2014-12-24 | 麦兰特公司 | 产生有机酸的大肠杆菌菌株的代谢进化 |
| WO2012019175A2 (en) * | 2010-08-06 | 2012-02-09 | Mascoma Corporation | Production of malonyl-coa drived products via anaerobic pathways |
| CA2862051A1 (en) * | 2012-01-30 | 2013-08-08 | Myriant Corporation | Production of muconic acid from genetically engineered microorganisms |
| CN102994436A (zh) * | 2012-10-29 | 2013-03-27 | 江南大学 | 一种产减毒类脂a的大肠杆菌基因工程菌及其应用 |
| JP6085190B2 (ja) * | 2013-02-26 | 2017-02-22 | 花王株式会社 | 変異微生物及びそれを用いた有用物質の生産方法 |
| KR101594156B1 (ko) * | 2013-06-25 | 2016-02-15 | 씨제이제일제당 주식회사 | Ｌ-라이신 생산능이 향상된 미생물 및 그를 이용하여 ｌ-라이신을 생산하는 방법 |
| US10035749B2 (en) | 2013-09-03 | 2018-07-31 | Myriant Corporation | Process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol |
| CN104046586B (zh) * | 2014-07-02 | 2016-08-17 | 山东大学 | 一株基因工程菌及其在生产(2r,3r)-2,3-丁二醇中的应用 |
| BR112017005665A2 (pt) * | 2014-09-18 | 2017-12-12 | Genomatica Inc | organismos microbianos não naturais com eficiência energética melhorada |
| US10301613B2 (en) | 2015-09-15 | 2019-05-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Targeted remodeling of prokaryotic genomes using CRISPR-nickases |
| WO2017151811A1 (en) | 2016-03-02 | 2017-09-08 | Myriant Corporation | Improved muconic acid production from genetically engineered microorganisms |
| AR111295A1 (es) | 2017-03-20 | 2019-06-26 | Forma Therapeutics Inc | Composiciones de pirrolopirrol como activadores de piruvato quinasa (pkr) |
| CA3068459A1 (en) | 2017-06-30 | 2019-01-03 | Ptt Global Chemical Public Company Limited | Microorganism with stabilized copy number of functional dna sequence and associated methods |
| US20200129485A1 (en) | 2018-09-19 | 2020-04-30 | Forma Therapeutics, Inc. | Treating sickle cell disease with a pyruvate kinase r activating compound |
| WO2020061255A1 (en) | 2018-09-19 | 2020-03-26 | Forma Therapeutics, Inc. | Activating pyruvate kinase r |
| CN109251941B (zh) * | 2018-09-30 | 2020-11-06 | 江南大学 | 一种高产琥珀酸的大肠杆菌及其应用 |
| KR102129379B1 (ko) * | 2018-10-10 | 2020-07-02 | 한국과학기술원 | 고활성의 말산 탈수소효소가 도입된 숙신산 생성용 변이 미생물 및 이를 이용한 숙신산 제조방법 |
| MX2022003025A (es) | 2019-09-19 | 2022-04-07 | Forma Therapeutics Inc | Activacion de la piruvato cinasa r. |
| CN113278641B (zh) * | 2020-05-27 | 2022-06-21 | 安徽华恒生物科技股份有限公司 | 生产l-缬氨酸的重组大肠杆菌、其构建方法及其应用 |
| IT202000013243A1 (it) | 2020-06-04 | 2021-12-04 | Novamont Spa | Processo per la purificazione di una miscela di dioli |
| IT202000031979A1 (it) | 2020-12-22 | 2022-06-22 | Novamont Spa | Processo di purificazione di una miscela comprendente dioli e acetali |
| IT202100006419A1 (it) | 2021-03-17 | 2022-09-17 | Novamont Spa | Recupero di dioli da una miscela |
| US12128035B2 (en) | 2021-03-19 | 2024-10-29 | Novo Nordisk Health Care Ag | Activating pyruvate kinase R |
| IT202100030572A1 (it) | 2021-12-02 | 2023-06-02 | Novamont Spa | 1,3-butandiolo purificato da una miscela di dioli |
| CN118546852B (zh) * | 2024-07-30 | 2024-10-18 | 北京化工大学 | 一种高效共利用葡萄糖、木糖生产丁二酸的基因工程菌及其应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008115958A2 (en) * | 2007-03-20 | 2008-09-25 | University Of Florida Research Foundation, Inc. | Materials and methods for efficient succinate and malate production |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0827542T3 (da) | 1995-05-05 | 2008-08-18 | Genencor Int | Anvendelse af glucose-transport-mutanter til fremstilling af forbindelser fra den aromatiske pathway |
| AU760575C (en) | 1998-04-13 | 2005-04-14 | University Of Georgia Research Foundation, Inc., The | Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells |
| US6989265B2 (en) | 2002-01-23 | 2006-01-24 | Wisconsin Alumni Research Foundation | Bacteria with reduced genome |
| US7303906B2 (en) | 2002-09-06 | 2007-12-04 | Wisconsin Alumni Research Foundation | Competent bacteria |
| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| US7232665B2 (en) | 2002-12-19 | 2007-06-19 | E. I. Du Pont De Nemours And Company | Mutations affecting carotenoid production |
| DE10314618A1 (de) | 2003-04-01 | 2004-10-14 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
| US8206955B2 (en) | 2003-10-21 | 2012-06-26 | Cargill, Incorporated | Production of monatin and monatin precursors |
| KR100576342B1 (ko) | 2004-02-05 | 2006-05-03 | 씨제이 주식회사 | ｇａｌＲ 유전자가 불활성화된 Ｌ-쓰레오닌 생성 미생물,그를 제조하는 방법 및 상기 미생물을 이용한Ｌ-쓰레오닌의 제조방법 |
| US20050181488A1 (en) | 2004-02-12 | 2005-08-18 | Akhverdian Valery Z. | Method for producing L-threonine using bacteria belonging to the genus Escherichia |
| EP3130676A1 (en) * | 2004-08-27 | 2017-02-15 | Rice University | Mutant e. coli strain with increased succinic acid production |
| WO2006034156A2 (en) | 2004-09-17 | 2006-03-30 | Rice University | High succinate producing bacteria |
| BRPI0517908A (pt) | 2004-11-26 | 2008-10-14 | Kyowa Hakko Kogyo Kk | cepa mutante de e. coli, e, método para produzir uma substáncia útil |
| KR100630836B1 (ko) | 2005-04-08 | 2006-10-02 | 한국과학기술원 | 인-실리코 분석을 통한 균주 개량방법 |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| ES2401339T3 (es) | 2005-08-10 | 2013-04-18 | University Of Florida Research Foundation, Inc. | Materiales y métodos para producción eficiente de ácido láctico |
| CA2626900C (en) | 2005-08-20 | 2013-07-23 | Scarab Genomics, Llc | Reduced genome e. coli |
| CN101300356A (zh) | 2005-09-09 | 2008-11-05 | 基因组股份公司 | 用于琥珀酸盐之生长关联性生产的方法和生物 |
| DE102005047596A1 (de) | 2005-10-05 | 2007-04-12 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| US7981659B2 (en) | 2005-10-13 | 2011-07-19 | Kao Corporation | Bacillus subtilis mutant strain |
| BRPI0709679B1 (pt) * | 2006-03-31 | 2017-03-28 | Rice Univ | método para fermentar anaerobicamente glicerol para produzir um produto; e método de produção de 1,2-pdo |
| KR100885616B1 (ko) | 2006-06-26 | 2009-02-24 | 씨제이제일제당 (주) | 글리세롤을 이용한 아미노산의 생산 방법 |
| WO2008116851A1 (en) | 2007-03-23 | 2008-10-02 | Metabolic Explorer | Metabolically engineered microorganism useful for the production of acetol |
| EP2139985A1 (en) | 2007-03-23 | 2010-01-06 | Metabolic Explorer | Metabolically engineered microorganism useful for the production of 1,2-propanediol |
| US20110039327A1 (en) * | 2007-05-18 | 2011-02-17 | Aaron Adriaan Winkler | Organic acid production by fungal cells |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| WO2009043372A1 (en) | 2007-10-02 | 2009-04-09 | Metabolic Explorer | Increasing methionine yield |
| WO2009061429A2 (en) * | 2007-11-06 | 2009-05-14 | Massachusetts Institute Of Technology | Global transcription machinery engineering targeting the rnap alpha subunit (rpoa) |
| WO2009072562A1 (ja) | 2007-12-06 | 2009-06-11 | Ajinomoto Co., Inc. | 有機酸の製造方法 |
| BRPI0921734A2 (pt) | 2008-10-31 | 2019-01-08 | California Inst Of Techn | micro-organismos criados geneticamente capazes de produzir compostos-alvo sob condições anaeróbicas |
| EP2414505B1 (en) | 2009-04-02 | 2019-05-22 | University of Florida Research Foundation, Inc. | Engineering the pathway for succinate production |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008115958A2 (en) * | 2007-03-20 | 2008-09-25 | University Of Florida Research Foundation, Inc. | Materials and methods for efficient succinate and malate production |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016528893A (ja) * | 2013-07-23 | 2016-09-23 | ミリアント・コーポレイションＭｙｒｉａｎｔ Ｃｏｒｐｏｒａｔｉｏｎ | 糖の取り込みのための促進拡散を用いるコハク酸および他の化合物の生産方法 |
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| CN102666838B (zh) | 2018-04-10 |
| CN102666838A (zh) | 2012-09-12 |
| KR20120099258A (ko) | 2012-09-07 |
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| EP2501797A4 (en) | 2013-04-17 |
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| EP2501797A2 (en) | 2012-09-26 |
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| WO2011063055A2 (en) | 2011-05-26 |
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