JP2013511277A - 化学物質の効率的な生産のための微生物の改変 - Google Patents

化学物質の効率的な生産のための微生物の改変 Download PDF

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JP2013511277A
JP2013511277A JP2012540031A JP2012540031A JP2013511277A JP 2013511277 A JP2013511277 A JP 2013511277A JP 2012540031 A JP2012540031 A JP 2012540031A JP 2012540031 A JP2012540031 A JP 2012540031A JP 2013511277 A JP2013511277 A JP 2013511277A
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gene
strain
naturally occurring
succinic acid
mutations
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JP2013511277A5 (enExample
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ゴン・ウェイ
スドハンシュ・ドール
タミー・グレイバー
アンドリュー・クリストファー・コラード
ジャニス・ジー・ペロ
アール・ロジャース・ヨキュム
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Myriant Corp
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JP2012540031A 2009-11-18 2010-11-17 化学物質の効率的な生産のための微生物の改変 Pending JP2013511277A (ja)

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US28148109P 2009-11-18 2009-11-18
US61/281,481 2009-11-18
PCT/US2010/057119 WO2011063055A2 (en) 2009-11-18 2010-11-17 Engineering microbes for efficient production of chemicals

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US (1) US9017976B2 (enExample)
EP (1) EP2501797A4 (enExample)
JP (1) JP2013511277A (enExample)
KR (2) KR20120099258A (enExample)
CN (1) CN102666838B (enExample)
BR (1) BR112012011990A2 (enExample)
WO (1) WO2011063055A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528893A (ja) * 2013-07-23 2016-09-23 ミリアント・コーポレイションMyriant Corporation 糖の取り込みのための促進拡散を用いるコハク酸および他の化合物の生産方法

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120103645A (ko) * 2009-11-18 2012-09-19 미리안트 코포레이션 유기산들을 생산하는 대장균의 대사적 진화
WO2012019175A2 (en) 2010-08-06 2012-02-09 Mascoma Corporation Production of malonyl-coa drived products via anaerobic pathways
JP2015504688A (ja) * 2012-01-30 2015-02-16 ミリアント・コーポレイションMyriant Corporation 遺伝子操作された微生物からのムコン酸の生成
CN102994436A (zh) * 2012-10-29 2013-03-27 江南大学 一种产减毒类脂a的大肠杆菌基因工程菌及其应用
JP6085190B2 (ja) * 2013-02-26 2017-02-22 花王株式会社 変異微生物及びそれを用いた有用物質の生産方法
KR101594156B1 (ko) 2013-06-25 2016-02-15 씨제이제일제당 주식회사 L-라이신 생산능이 향상된 미생물 및 그를 이용하여 l-라이신을 생산하는 방법
EP3041942B1 (en) 2013-09-03 2020-07-01 PTT Global Chemical Public Company Limited A process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol
CN104046586B (zh) * 2014-07-02 2016-08-17 山东大学 一株基因工程菌及其在生产(2r,3r)-2,3-丁二醇中的应用
CN107208118A (zh) * 2014-09-18 2017-09-26 基因组股份公司 具有提高的能源效率的非天然微生物
US10301613B2 (en) 2015-09-15 2019-05-28 Arizona Board Of Regents On Behalf Of Arizona State University Targeted remodeling of prokaryotic genomes using CRISPR-nickases
EP3423563B1 (en) 2016-03-02 2023-05-24 PTT Global Chemical Public Company Limited Improved muconic acid production from genetically engineered microorganisms
MD3448859T2 (ro) 2017-03-20 2020-03-31 Forma Therapeutics Inc Compoziții de pirolopirol în calitate de activatori ai piruvatkinazei (PKR)
KR102683624B1 (ko) 2017-06-30 2024-07-11 피티티 글로벌 케미칼 피씨엘 기능적 dna 서열의 안정화된 카피 수를 갖는 미생물 및 관련 방법
WO2020061378A1 (en) 2018-09-19 2020-03-26 Forma Therapeutics, Inc. Treating sickle cell disease with a pyruvate kinase r activating compound
ES2989438T3 (es) 2018-09-19 2024-11-26 Novo Nordisk Healthcare Ag Activación de la piruvato cinasa R
CN109251941B (zh) * 2018-09-30 2020-11-06 江南大学 一种高产琥珀酸的大肠杆菌及其应用
KR102129379B1 (ko) * 2018-10-10 2020-07-02 한국과학기술원 고활성의 말산 탈수소효소가 도입된 숙신산 생성용 변이 미생물 및 이를 이용한 숙신산 제조방법
AU2020350763A1 (en) 2019-09-19 2022-04-07 Novo Nordisk Health Care Ag Pyruvate kinase R (PKR) activating compositions
CN113278641B (zh) * 2020-05-27 2022-06-21 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组大肠杆菌、其构建方法及其应用
IT202000013243A1 (it) 2020-06-04 2021-12-04 Novamont Spa Processo per la purificazione di una miscela di dioli
IT202000031979A1 (it) 2020-12-22 2022-06-22 Novamont Spa Processo di purificazione di una miscela comprendente dioli e acetali
IT202100006419A1 (it) 2021-03-17 2022-09-17 Novamont Spa Recupero di dioli da una miscela
US12128035B2 (en) 2021-03-19 2024-10-29 Novo Nordisk Health Care Ag Activating pyruvate kinase R
IT202100030572A1 (it) 2021-12-02 2023-06-02 Novamont Spa 1,3-butandiolo purificato da una miscela di dioli
CN118546852B (zh) * 2024-07-30 2024-10-18 北京化工大学 一种高效共利用葡萄糖、木糖生产丁二酸的基因工程菌及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008115958A2 (en) * 2007-03-20 2008-09-25 University Of Florida Research Foundation, Inc. Materials and methods for efficient succinate and malate production

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1947186B1 (en) 1995-05-05 2011-11-16 Danisco US Inc. Application of glucose transport mutants for production of aromatic pathway compounds
MY121380A (en) 1998-04-13 2006-01-28 Univ Georgia Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
KR101148255B1 (ko) 2002-10-04 2012-08-08 다니스코 유에스 인크. 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법
US7232665B2 (en) 2002-12-19 2007-06-19 E. I. Du Pont De Nemours And Company Mutations affecting carotenoid production
DE10314618A1 (de) 2003-04-01 2004-10-14 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
CN103555782B (zh) 2003-10-21 2017-04-12 嘉吉有限公司 生产莫纳甜和莫纳甜前体
KR100576342B1 (ko) 2004-02-05 2006-05-03 씨제이 주식회사 galR 유전자가 불활성화된 L-쓰레오닌 생성 미생물,그를 제조하는 방법 및 상기 미생물을 이용한L-쓰레오닌의 제조방법
US20050181488A1 (en) 2004-02-12 2005-08-18 Akhverdian Valery Z. Method for producing L-threonine using bacteria belonging to the genus Escherichia
KR101245428B1 (ko) 2004-08-27 2013-03-19 라이스 유니버시티 숙신산 생산이 증가된 돌연변이 대장균 균주
US20060073577A1 (en) 2004-09-17 2006-04-06 San Ka-Yiu High succinate producing bacteria
US20080009041A1 (en) 2004-11-26 2008-01-10 Hiroshi Mizoguchi Industrially Useful Microorganism
KR100630836B1 (ko) 2005-04-08 2006-10-02 한국과학기술원 인-실리코 분석을 통한 균주 개량방법
US7524660B2 (en) 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
EP2305826B1 (en) 2005-08-10 2016-03-30 University of Florida Research Foundation, Inc. Materials and methods for efficient lactic acid production
US8178339B2 (en) 2005-08-20 2012-05-15 Scarab Genomics Llc Reduced genome E. coli
EP1937821A4 (en) 2005-09-09 2009-11-11 Genomatica Inc PROCESS AND ORGANISMS FOR GROWTH-COUPLED PRODUCTION OF SUCCINATE
DE102005047596A1 (de) 2005-10-05 2007-04-12 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
DK1944365T3 (da) 2005-10-13 2012-04-30 Kao Corp Hidtil ukendt bacillus subtilis mutantstamme
KR101390085B1 (ko) * 2006-03-31 2014-04-28 라이스 유니버시티 글리세롤의 혐기성 발효
KR100885616B1 (ko) 2006-06-26 2009-02-24 씨제이제일제당 (주) 글리세롤을 이용한 아미노산의 생산 방법
JP2010521190A (ja) 2007-03-23 2010-06-24 メタボリック エクスプローラー 1,2−プロパンジオールの製造に有用な、代謝的に操作された微生物
US20100279369A1 (en) 2007-03-23 2010-11-04 Metabolic Explorer Metabolically engineered microorganism useful for the production of acetol
US20110039327A1 (en) * 2007-05-18 2011-02-17 Aaron Adriaan Winkler Organic acid production by fungal cells
US7947483B2 (en) 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
WO2009043372A1 (en) 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
US20100330614A1 (en) * 2007-11-06 2010-12-30 Massachusetts Institute Of Technology Global transcription machinery engineering targeting the rnap alpha subunit (rpoa)
EP2241630B1 (en) 2007-12-06 2016-06-01 Ajinomoto Co., Inc. A method for producing an organic acid
CA2779262C (en) 2008-10-31 2021-09-07 Gevo, Inc. Engineered microorganisms capable of converting pyruvate to isobutanol under anaerobic conditions
KR20120025450A (ko) 2009-04-02 2012-03-15 유니버시티 오브 플로리다 리서치 파운데이션, 인크. 석시네이트 생성을 위한 경로의 엔지니어링

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008115958A2 (en) * 2007-03-20 2008-09-25 University Of Florida Research Foundation, Inc. Materials and methods for efficient succinate and malate production

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528893A (ja) * 2013-07-23 2016-09-23 ミリアント・コーポレイションMyriant Corporation 糖の取り込みのための促進拡散を用いるコハク酸および他の化合物の生産方法

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CN102666838B (zh) 2018-04-10
KR20120099258A (ko) 2012-09-07
WO2011063055A2 (en) 2011-05-26
EP2501797A2 (en) 2012-09-26
EP2501797A4 (en) 2013-04-17
BR112012011990A2 (pt) 2015-09-29
WO2011063055A3 (en) 2011-11-17
US20120220000A1 (en) 2012-08-30
US9017976B2 (en) 2015-04-28
CN102666838A (zh) 2012-09-12
KR20160114184A (ko) 2016-10-04

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