KR20120088716A - 생물학적 샘플 내의 다수의 표적의 검출 - Google Patents

생물학적 샘플 내의 다수의 표적의 검출 Download PDF

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KR20120088716A
KR20120088716A KR1020127010261A KR20127010261A KR20120088716A KR 20120088716 A KR20120088716 A KR 20120088716A KR 1020127010261 A KR1020127010261 A KR 1020127010261A KR 20127010261 A KR20127010261 A KR 20127010261A KR 20120088716 A KR20120088716 A KR 20120088716A
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target
signal
probes
targets
biological sample
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Korean (ko)
Inventor
애넙 수드
존 리차드 넬슨
마이클 저디스
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제너럴 일렉트릭 캄파니
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020127010261A 2009-10-21 2010-10-21 생물학적 샘플 내의 다수의 표적의 검출 Withdrawn KR20120088716A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/582,745 US9677125B2 (en) 2009-10-21 2009-10-21 Detection of plurality of targets in biological samples
US12/582,745 2009-10-21
PCT/EP2010/065876 WO2011048184A1 (en) 2009-10-21 2010-10-21 Detection of plurality of targets in biological samples

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KR20120088716A true KR20120088716A (ko) 2012-08-08

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KR1020127010261A Withdrawn KR20120088716A (ko) 2009-10-21 2010-10-21 생물학적 샘플 내의 다수의 표적의 검출

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US (1) US9677125B2 (enExample)
EP (1) EP2491132B1 (enExample)
JP (1) JP2013507941A (enExample)
KR (1) KR20120088716A (enExample)
CN (1) CN102803510A (enExample)
AU (1) AU2010309739A1 (enExample)
BR (1) BR112012009481A2 (enExample)
CA (1) CA2777421A1 (enExample)
IN (1) IN2012DN03156A (enExample)
RU (1) RU2012115681A (enExample)
WO (1) WO2011048184A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
US8568991B2 (en) 2011-12-23 2013-10-29 General Electric Company Photoactivated chemical bleaching of dyes
US9176032B2 (en) 2011-12-23 2015-11-03 General Electric Company Methods of analyzing an H and E stained biological sample
KR101460439B1 (ko) * 2012-05-14 2014-11-12 서울대학교산학협력단 나노프로브 및 이를 이용한 표적 물질 검출방법
WO2013187765A2 (en) * 2012-06-14 2013-12-19 Erasmus University Medical Center Rotterdam Methods, reagents and kits for detecting minimal residual disease
WO2014099222A1 (en) * 2012-12-17 2014-06-26 Clarient Diagnostic Services, Inc. Chemical bleaching of dyes using radical photoinitiators
JP2016513794A (ja) 2013-03-06 2016-05-16 ゼネラル・エレクトリック・カンパニイ H&e染色された生体試料を分析する方法
US10760064B2 (en) 2013-03-15 2020-09-01 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
WO2014204578A1 (en) 2013-06-21 2014-12-24 The General Hospital Corporation Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing
KR102271292B1 (ko) 2013-03-15 2021-07-02 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도
US10457980B2 (en) 2013-04-30 2019-10-29 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding
CA2919833A1 (en) 2013-07-30 2015-02-05 President And Fellows Of Harvard College Quantitative dna-based imaging and super-resolution imaging
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US10294510B2 (en) * 2014-03-11 2019-05-21 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
EP4273263A3 (en) 2014-07-30 2024-01-31 President and Fellows of Harvard College Systems and methods for determining nucleic acids
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
US9926546B2 (en) 2015-08-28 2018-03-27 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
WO2018022809A1 (en) 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
CN106290877A (zh) * 2016-09-08 2017-01-04 北京海思特临床检验所有限公司 急性髓系白血病粒细胞抗原表达量检测试剂盒及检测方法
CN106290812A (zh) * 2016-09-08 2017-01-04 北京海思特临床检验所有限公司 B细胞恶性肿瘤相关抗原表达量检测试剂盒及检测方法
KR102606620B1 (ko) 2016-12-09 2023-11-28 얼티뷰, 인크. 표지된 핵산 조영제를 사용한 다중 이미지형성을 위한 개선된 방법
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
EP3717009A4 (en) * 2017-11-30 2021-12-01 Arrakis Therapeutics, Inc. NUCLEIC ACID BINDING PHOTOSONDES AND THEIR USES
JP6999527B2 (ja) * 2018-09-25 2022-01-18 株式会社日立ハイテク 試験方法、分注装置
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
WO2020123742A1 (en) 2018-12-13 2020-06-18 President And Fellows Of Harvard College Amplification methods and systems for merfish and other applications
US11397179B2 (en) 2018-12-31 2022-07-26 Robert Bosch Gmbh PH-modulated imaging of targets close to a solid surface
JP7405400B2 (ja) * 2019-11-20 2023-12-26 Necソリューションイノベータ株式会社 核酸分子およびその用途
EP4168575A1 (en) 2020-06-18 2023-04-26 Resolve BioSciences GmbH Multiplex method for detecting different analytes in a sample
EP4322837A4 (en) 2021-04-16 2025-02-26 Advanced Cell Diagnostics, Inc. MATERIALS AND METHODS RELATED TO IMAGE PROCESSING
CA3228621A1 (en) * 2021-08-09 2023-02-16 Xin Huang Compositions, kits, and methods for detecting preclinical alzheimer's disease
EP4474793A1 (en) * 2023-06-09 2024-12-11 Leica Biosystems Richmond, Inc. Automated melanin bleaching

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405720A (en) 1981-03-04 1983-09-20 The United States Of America As Represented By The Department Of Health And Human Services Silver stains for protein in gels
GB8405437D0 (en) 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
JPH01196567A (ja) * 1988-02-01 1989-08-08 Hitachi Ltd 核酸検査方法及び核酸検査装置
JP3130513B2 (ja) 1989-04-19 2001-01-31 イビデン株式会社 生体活性物質測定用装置
US5401469A (en) 1989-04-19 1995-03-28 Ibiden Co., Ltd. Plastic optical biomaterials assay device
JP3188303B2 (ja) * 1992-04-10 2001-07-16 株式会社日立製作所 ポリヌクレオチド検出法
US5843640A (en) 1992-06-19 1998-12-01 Northwestern University Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells
WO1994009022A1 (en) 1992-10-09 1994-04-28 Oncor, Inc. Methods for the detection of chromosome structural abnormalities by in situ hybridization to fixed tissue
US5776755A (en) 1992-10-09 1998-07-07 Helsinki University Licensing, Ltd. FLT4, a receptor tyrosine kinase
EP0712444A1 (en) 1993-07-20 1996-05-22 University Of Massachusetts Medical Center In vivo nucleic acid hybridization method
FR2711671A1 (fr) 1993-10-22 1995-05-05 Centre Nat Rech Scient Procédé de détection et de quantification d'une séquence nucléotidique dans une population cellulaire.
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
DE4421891C2 (de) 1994-06-23 1996-05-15 Helmut Prof Dr Med Feucht Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene
US5571643A (en) 1994-07-22 1996-11-05 Martin; Mark T. Spectrophotometric quantitation of images in X-ray film and electrophoresis gel bands
ATE340866T1 (de) 1994-10-28 2006-10-15 Gen Probe Inc Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5763152A (en) 1995-03-31 1998-06-09 Fuji Photo Film Co., Ltd. Silver halide photographic light-sensitive material
EP0801306A1 (en) 1996-04-10 1997-10-15 Becton, Dickinson and Company Method for immunohistochemically quantitating co-localized molecules
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
AU3717797A (en) 1996-07-15 1998-02-09 Children's Medical Center Corporation Method to co-detect introduced genes and their products
US6735531B2 (en) 1996-10-07 2004-05-11 Lab Vision Corporation Method and apparatus for automatic tissue staining
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
US6083486A (en) 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
JP2002520448A (ja) 1998-07-10 2002-07-09 クロマジェン インコーポレーティッド 加水分解酵素の新規蛍光原基質
DE19835284C2 (de) 1998-08-05 2001-03-01 Tuhh Tech Gmbh Vorrichtung und Verfahren zur automatischen Fokussierung und Belichtung von Bildaufnahmesystemen
CA2340361A1 (en) 1998-08-21 2000-03-16 Peter C. Cheng Assays using crosslinkable immobilized nucleic acids
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
DE69942981D1 (de) 1998-10-09 2011-01-05 Vegenics Ltd Flt4 (vegfr-3) als ziel für tumornachweis (imaging) und antitumortherapie
US6087112A (en) 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
US6391649B1 (en) 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
US20020106648A1 (en) 2000-05-05 2002-08-08 Lizardi Paul M. Highly multiplexed reporter carrier systems
IL136232A0 (en) 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
US20030044353A1 (en) 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20020114742A1 (en) 2001-02-20 2002-08-22 Hitachi, Ltd. Test chamber
JP2002323416A (ja) 2001-02-20 2002-11-08 Hitachi Ltd 検査容器
WO2002079771A1 (en) 2001-03-30 2002-10-10 The Samuel Roberts Noble Foundation, Inc. Silver destaining method
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US20020173053A1 (en) 2001-04-27 2002-11-21 Bassam Damaj Multiple simultaneous antigen detection by immunohistochemistry
JP3899845B2 (ja) 2001-05-23 2007-03-28 日本光電工業株式会社 染色標本の脱色方法および装置
US20040241688A1 (en) 2001-07-19 2004-12-02 Cuneyt Bukusoglu Human tissue specific drug screening procedure
DE10143757A1 (de) 2001-09-06 2003-03-27 Werner M Methode zur Darstellung von Merkmalen in Geweben
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
WO2003061711A2 (en) 2002-01-16 2003-07-31 Visen Medical, Inc. Chromophore probes for optical imaging
US6731831B2 (en) 2002-02-27 2004-05-04 Xiang Zheng Tu Optical switch array assembly for DNA probe synthesis and detection
US7776195B2 (en) 2002-05-28 2010-08-17 Autogenomics, Inc. Integrated sample processing platform
JP2004041121A (ja) 2002-07-12 2004-02-12 Hitachi Ltd アレルギー解析方法及びシステム
WO2004061446A1 (en) 2002-12-18 2004-07-22 Aclara Biosciences, Inc. Single cell analysis of membrane molecules
US20040241776A1 (en) 2003-05-22 2004-12-02 Agdia, Inc. Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes
JP3936689B2 (ja) 2003-09-09 2007-06-27 日本碍子株式会社 シアニン系有機色素劣化環境の検知方法
US7463778B2 (en) 2004-01-30 2008-12-09 Hewlett-Packard Development Company, L.P Motion estimation for compressing multiple view images
JP4538628B2 (ja) 2004-03-16 2010-09-08 学校法人慶應義塾 遺伝子発現検出方法
JP4189495B2 (ja) 2004-08-02 2008-12-03 国立大学法人群馬大学 ゲノムdnaのメチル化検出方法
EP1836322B8 (en) * 2005-01-13 2017-01-11 Progenika Biopharma, S.A. Methods and products for in vitro genotyping
US7465561B2 (en) * 2005-06-30 2008-12-16 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using single probe analysis
JP2007033159A (ja) 2005-07-25 2007-02-08 Assay Corp 蛍光分子で標識された測定対象物質
WO2007055302A1 (ja) 2005-11-10 2007-05-18 National University Corporation University Of Toyama 生体物質と生体物質結合性物質との結合性評価方法
US7629125B2 (en) 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US7741045B2 (en) 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US8305579B2 (en) 2006-11-16 2012-11-06 Thomas Pirrie Treynor Sequential analysis of biological samples
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
JP2009232707A (ja) * 2008-03-26 2009-10-15 Canon Inc 一塩基多型の検出法及びプローブ固定担体

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Publication number Publication date
WO2011048184A1 (en) 2011-04-28
JP2013507941A (ja) 2013-03-07
CA2777421A1 (en) 2011-04-28
CN102803510A (zh) 2012-11-28
BR112012009481A2 (pt) 2016-11-29
EP2491132B1 (en) 2018-03-21
RU2012115681A (ru) 2013-11-27
EP2491132A1 (en) 2012-08-29
US9677125B2 (en) 2017-06-13
AU2010309739A1 (en) 2012-05-03
IN2012DN03156A (enExample) 2015-09-18
US20110092381A1 (en) 2011-04-21

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Patent event date: 20120420

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid