KR20050095605A - 폴리펩티드의 정제 - Google Patents

폴리펩티드의 정제 Download PDF

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Publication number
KR20050095605A
KR20050095605A KR1020057012831A KR20057012831A KR20050095605A KR 20050095605 A KR20050095605 A KR 20050095605A KR 1020057012831 A KR1020057012831 A KR 1020057012831A KR 20057012831 A KR20057012831 A KR 20057012831A KR 20050095605 A KR20050095605 A KR 20050095605A
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KR
South Korea
Prior art keywords
polypeptide
homogenate
lactate
broth
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020057012831A
Other languages
English (en)
Korean (ko)
Inventor
필립 엠. 레스터
조세핀 퍼슨
Original Assignee
제넨테크, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 제넨테크, 인크. filed Critical 제넨테크, 인크.
Publication of KR20050095605A publication Critical patent/KR20050095605A/ko
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020057012831A 2003-01-09 2004-01-08 폴리펩티드의 정제 Ceased KR20050095605A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43941803P 2003-01-09 2003-01-09
US60/439,418 2003-01-09

Publications (1)

Publication Number Publication Date
KR20050095605A true KR20050095605A (ko) 2005-09-29

Family

ID=33299572

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020057012831A Ceased KR20050095605A (ko) 2003-01-09 2004-01-08 폴리펩티드의 정제

Country Status (18)

Country Link
US (2) US7169908B2 (https=)
EP (1) EP1581644B1 (https=)
JP (1) JP2006517415A (https=)
KR (1) KR20050095605A (https=)
CN (1) CN100467611C (https=)
AT (1) ATE364092T1 (https=)
AU (1) AU2004230670B2 (https=)
BR (1) BRPI0406470A (https=)
CA (1) CA2511946A1 (https=)
DE (1) DE602004006831T2 (https=)
DK (1) DK1581644T3 (https=)
ES (1) ES2287687T3 (https=)
MX (1) MXPA05007378A (https=)
NZ (1) NZ540895A (https=)
PL (1) PL377653A1 (https=)
RU (1) RU2337968C2 (https=)
WO (1) WO2004092393A1 (https=)
ZA (1) ZA200504990B (https=)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2245114T3 (es) 1998-08-06 2005-12-16 Mountain View Pharmaceuticals, Inc. Conjugados de peg-oxidasa de urato y su uso.
US6967241B2 (en) * 2002-09-06 2005-11-22 Genentech, Inc. Process for protein extraction
HUE052976T2 (hu) 2005-04-11 2021-06-28 Horizon Pharma Rheumatology Llc Urát-oxidáz variáns formái és azok alkalmazása
TWI418564B (zh) * 2005-04-11 2013-12-11 Savient Pharmaceuticals Inc 利用陽離子界面活性劑之蛋白質純化
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
CN101622270B (zh) * 2006-04-12 2014-01-01 萨文特医药公司 用阳离子表面活性剂纯化蛋白质的方法
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
CN101611145B (zh) * 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
NZ577933A (en) 2007-01-22 2011-12-22 Genentech Inc Polyelectrolyte precipitation and purification of antibodies
KR20190140090A (ko) 2007-07-09 2019-12-18 제넨테크, 인크. 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지
US7887821B2 (en) * 2007-12-20 2011-02-15 Alk-Abello A/S Process for producing an allergen extract
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
GB0818228D0 (en) * 2008-10-06 2008-11-12 Avecia Biolog Ltd Purification process
EP2337787A1 (en) * 2008-10-17 2011-06-29 Percivia LLC. Clarification process
CN102257122B (zh) 2008-12-16 2015-07-29 Emd密理博公司 搅拌槽反应器及方法
HUP1200205A3 (en) 2009-06-25 2012-09-28 Savient Pharmaceuticals Method and kits for perdicting infusion reaction risk and antibody-mediated low of response by monitoring serum uric acid during pegylated uricare therapy
CA2788607C (en) * 2010-02-01 2018-03-20 Digna Biotech,S.L. Process for the production of interferon alpha 5
US9884111B2 (en) * 2010-02-12 2018-02-06 Laboratorios Leti, S.L. Process for producing an allergen extract
US9884904B2 (en) * 2010-03-10 2018-02-06 Hoffmann-La Roche Inc. Method for purifying polypeptide solutions
JO3131B1 (ar) 2010-04-27 2017-09-20 Glaxosmithkline Llc مركبات كيميائية
CN107312062B (zh) 2010-05-17 2021-03-16 Emd密理博公司 用于纯化生物分子的刺激响应性聚合物
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
DE102011001743A1 (de) * 2011-04-01 2012-10-04 Technische Universität Dortmund Verfahren zur Trennung/Reinigung von Biomolekühlen
CN107266577B (zh) * 2011-10-11 2022-09-13 弗·哈夫曼-拉罗切有限公司 双特异性抗体的改进的组装
CN102443055B (zh) * 2011-10-26 2017-07-28 浙江海正药业股份有限公司 一种重组人肿瘤坏死因子相关凋亡诱导配体的纯化工艺
SG11201407801VA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations
CN102701501A (zh) * 2012-06-26 2012-10-03 天津市津华盛生物科技有限公司 工业乳酸链球菌素废水综合利用的方法
KR20150118103A (ko) * 2013-02-06 2015-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 정제 방법
SG11201505203XA (en) * 2013-02-26 2015-08-28 Agency Science Tech & Res Protein purification in the presence of nonionic organic polymers and electropositive surfaces
JP2016509068A (ja) * 2013-02-28 2016-03-24 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ クロマチン欠損細胞培養採取物からの抗体のクロマトグラフィー精製
CN105008384A (zh) 2013-02-28 2015-10-28 新加坡科技研究局 在高电导率下在非离子性有机聚合物的存在下的蛋白质纯化
RU2646098C2 (ru) * 2013-04-30 2018-03-01 Интас Фармасьютикалс Лтд Новый способ клонирования, экспрессии и очистки для получения ранибизумаба
WO2015166037A1 (en) * 2014-04-30 2015-11-05 Novozymes A/S Method for reducing the dna content of a fermentation broth
CN110234340A (zh) 2016-11-11 2019-09-13 好利恩风湿病制药有限责任公司 泼尼松和尿酸酶分子的组合疗法及其用途
US20200237881A1 (en) 2019-01-30 2020-07-30 Horizon Pharma Rheumatology Llc Reducing immunogenicity to pegloticase
CN108333263A (zh) * 2017-01-20 2018-07-27 北京蛋白质组研究中心 一种尿蛋白制备方法及尿蛋白质组的检测方法
TW201927335A (zh) * 2017-10-02 2019-07-16 美商拜耳保健有限責任公司 防止雙硫鍵於細胞培養收集物中還原之方法
BR112020010615A2 (pt) * 2017-12-22 2020-10-27 Regeneron Pharmaceuticals, Inc. produto farmacêutico proteico, método e sistema para caracterizar impurezas de produto farmacêutico proteico e de fármaco de baixo peso molecular, método para produzir um anticorpo, anticorpo, e, usos do método e do sistema
CN111363024A (zh) * 2018-12-26 2020-07-03 深圳翰宇药业股份有限公司 多肽的纯化方法
WO2020247983A1 (en) * 2019-06-05 2020-12-10 Viome, Inc. Sample collection methods and devices
EP4632063A3 (en) 2020-04-15 2025-10-22 Genentech, Inc. Copper loss mitigation
CN112708566A (zh) * 2021-01-25 2021-04-27 西南林业大学 一种黑曲霉生产胞外多聚物的优化培养基及培养方法
CN114774500A (zh) * 2022-04-26 2022-07-22 成都蜀星饲料有限公司 多肽及制备方法、有机复合微量元素及制备方法
US12269875B2 (en) 2023-08-03 2025-04-08 Jeff R. Peterson Gout flare prevention methods using IL-1BETA blockers

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL34079A (en) * 1969-04-01 1973-02-28 Upjohn Co Purification of ypsilon-globulins
US3607857A (en) * 1970-03-03 1971-09-21 Upjohn Co Process of removing acrinol from gamma globulin using siliceous material such as silica gel
US3903254A (en) * 1971-07-23 1975-09-02 Upjohn Co Separation of erythrocyte stroma from lysing medium and hemoglobin with acrinol
DE2308013A1 (de) * 1973-02-17 1974-09-12 Behringwerke Ag Katalasearme glucoseoxidase und verfahren zu ihrer gewinnung
JPS519035B2 (https=) * 1973-10-31 1976-03-23
SU944580A1 (ru) 1980-02-07 1982-07-23 Кировский научно-исследовательский институт переливания крови Способ получени поверхностного антигена гепатита В
CA1237998A (en) * 1984-04-14 1988-06-14 Akihiro Ginnaga Method for purification of filamentous hemagglutinin
JPS60258127A (ja) * 1984-06-04 1985-12-20 Green Cross Corp:The Ｂ型肝炎ワクチンの製造方法
EP0226639B1 (en) * 1985-05-29 1990-11-14 The Green Cross Corporation Process for preparing heterogenic protein
EP0227833B1 (en) * 1985-07-16 1991-01-16 Green Cross Corporation Process for preparing hetero-protein
DE3604947A1 (de) 1986-02-17 1987-08-20 Biotest Pharma Gmbh Verfahren zur herstellung eines immunglobulinhaltigen praeparates und dessen verwendung zur prophylaxe und therapie von aids
FR2600078B1 (fr) 1986-06-12 1989-07-13 Merieux Inst Procede de preparation de facteur xiii a partir du placenta
DE3929504A1 (de) * 1989-09-06 1991-03-07 Behringwerke Ag Verfahren zur reinigung von plasminogen-aktivator-inhibitor 2 (pai-2)
DE4030264A1 (de) * 1990-09-25 1992-04-23 Hoechst Ag Verfahren zur herstellung gereinigter glycolipide durch membrantrennverfahren
GB9215540D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5861263A (en) * 1992-10-20 1999-01-19 Universidad Autonoma De Nuevo Leon Preparation of preserved entamoeba histolytica antigens without enzymatic inhibitors and their use in immunological methods
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5760189A (en) 1995-06-02 1998-06-02 Genetics Institute, Inc. Protein recovery & purification methods
US5714583A (en) 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
CA2289665C (en) 1997-06-13 2005-08-09 Genentech, Inc. Protein recovery by chromatography followed by filtration upon a charged layer
JPH11341947A (ja) * 1998-06-02 1999-12-14 Yakult Honsha Co Ltd 発酵管理方法
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
ATE316097T1 (de) * 2000-01-20 2006-02-15 Univ Minnesota Peptide mit antibakterieller wirkung

Also Published As

Publication number Publication date
ZA200504990B (en) 2006-08-30
BRPI0406470A (pt) 2005-12-06
JP2006517415A (ja) 2006-07-27
DK1581644T3 (da) 2007-10-08
EP1581644B1 (en) 2007-06-06
US7169908B2 (en) 2007-01-30
ES2287687T3 (es) 2007-12-16
EP1581644A1 (en) 2005-10-05
AU2004230670B2 (en) 2010-11-25
CA2511946A1 (en) 2004-10-28
ATE364092T1 (de) 2007-06-15
DE602004006831D1 (de) 2007-07-19
WO2004092393A1 (en) 2004-10-28
US7579448B2 (en) 2009-08-25
MXPA05007378A (es) 2005-11-23
US20070077625A1 (en) 2007-04-05
AU2004230670A1 (en) 2004-10-28
US20050037456A1 (en) 2005-02-17
RU2005125210A (ru) 2006-01-10
PL377653A1 (pl) 2006-02-06
CN100467611C (zh) 2009-03-11
CN1759186A (zh) 2006-04-12
DE602004006831T2 (de) 2008-02-14
NZ540895A (en) 2007-03-30
RU2337968C2 (ru) 2008-11-10

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