BRPI0406470A - Método para a purificação de polipeptìdeo heterólogo e caldo ou homogeneizado de fermentação - Google Patents

Método para a purificação de polipeptìdeo heterólogo e caldo ou homogeneizado de fermentação

Info

Publication number
BRPI0406470A
BRPI0406470A BR0406470-4A BRPI0406470A BRPI0406470A BR PI0406470 A BRPI0406470 A BR PI0406470A BR PI0406470 A BRPI0406470 A BR PI0406470A BR PI0406470 A BRPI0406470 A BR PI0406470A
Authority
BR
Brazil
Prior art keywords
homogenate
polypeptide
broth
purification
heterologous polypeptide
Prior art date
Application number
BR0406470-4A
Other languages
English (en)
Inventor
Philip M Lester
Josefine Persson
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BRPI0406470A publication Critical patent/BRPI0406470A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA A PURIFICAçãO DE POLIPEPTìDEO HETERóLOGO E CALDO OU HOMOGENEIZADO DE FERMENTAçãO". A presente invenção refere-se a um método para a purificação de um polipeptídeo heterólogo de interesse a partir de caldo ou homogeneizado de fermentação microbiana no qual é produzido e solubilizado. Este método envolve a adição ao caldo ou homogeneizado de quantidade eficaz de uma solução de lactato de 6,9-diamino-2-etoxiacridina (lactato de etacridina) para precipitar as impurezas da célula hospedeira sob condições em que a maior parte do polipeptídeo permanece solúvel e separação do polipeptídeo desejado do caldo ou homogeneizado. Também é descrito o caldo ou homogeneizado que contém o lactato de etacridina e polipeptídeo.
BR0406470-4A 2003-01-09 2004-01-08 Método para a purificação de polipeptìdeo heterólogo e caldo ou homogeneizado de fermentação BRPI0406470A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43941803P 2003-01-09 2003-01-09
PCT/US2004/000499 WO2004092393A1 (en) 2003-01-09 2004-01-08 Purification of polypeptides

Publications (1)

Publication Number Publication Date
BRPI0406470A true BRPI0406470A (pt) 2005-12-06

Family

ID=33299572

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0406470-4A BRPI0406470A (pt) 2003-01-09 2004-01-08 Método para a purificação de polipeptìdeo heterólogo e caldo ou homogeneizado de fermentação

Country Status (18)

Country Link
US (2) US7169908B2 (pt)
EP (1) EP1581644B1 (pt)
JP (1) JP2006517415A (pt)
KR (1) KR20050095605A (pt)
CN (1) CN100467611C (pt)
AT (1) ATE364092T1 (pt)
AU (1) AU2004230670B2 (pt)
BR (1) BRPI0406470A (pt)
CA (1) CA2511946A1 (pt)
DE (1) DE602004006831T2 (pt)
DK (1) DK1581644T3 (pt)
ES (1) ES2287687T3 (pt)
MX (1) MXPA05007378A (pt)
NZ (1) NZ540895A (pt)
PL (1) PL377653A1 (pt)
RU (1) RU2337968C2 (pt)
WO (1) WO2004092393A1 (pt)
ZA (1) ZA200504990B (pt)

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CN102443055B (zh) * 2011-10-26 2017-07-28 浙江海正药业股份有限公司 一种重组人肿瘤坏死因子相关凋亡诱导配体的纯化工艺
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Also Published As

Publication number Publication date
MXPA05007378A (es) 2005-11-23
EP1581644A1 (en) 2005-10-05
CN100467611C (zh) 2009-03-11
WO2004092393A1 (en) 2004-10-28
ATE364092T1 (de) 2007-06-15
KR20050095605A (ko) 2005-09-29
CN1759186A (zh) 2006-04-12
US20050037456A1 (en) 2005-02-17
DK1581644T3 (da) 2007-10-08
DE602004006831T2 (de) 2008-02-14
JP2006517415A (ja) 2006-07-27
ES2287687T3 (es) 2007-12-16
AU2004230670B2 (en) 2010-11-25
CA2511946A1 (en) 2004-10-28
US7169908B2 (en) 2007-01-30
AU2004230670A1 (en) 2004-10-28
PL377653A1 (pl) 2006-02-06
RU2005125210A (ru) 2006-01-10
DE602004006831D1 (de) 2007-07-19
US7579448B2 (en) 2009-08-25
US20070077625A1 (en) 2007-04-05
ZA200504990B (en) 2006-08-30
NZ540895A (en) 2007-03-30
RU2337968C2 (ru) 2008-11-10
EP1581644B1 (en) 2007-06-06

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]