DE602004006831T2 - Reinigung von polypeptiden - Google Patents

Reinigung von polypeptiden Download PDF

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Publication number
DE602004006831T2
DE602004006831T2 DE602004006831T DE602004006831T DE602004006831T2 DE 602004006831 T2 DE602004006831 T2 DE 602004006831T2 DE 602004006831 T DE602004006831 T DE 602004006831T DE 602004006831 T DE602004006831 T DE 602004006831T DE 602004006831 T2 DE602004006831 T2 DE 602004006831T2
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DE
Germany
Prior art keywords
antibody
polypeptide
homogenate
ethacridine lactate
broth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004006831T
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German (de)
English (en)
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DE602004006831D1 (de
Inventor
Philip M. San Lorenzo LESTER
Josefine Persson
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Genentech Inc
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Genentech Inc
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Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE602004006831D1 publication Critical patent/DE602004006831D1/de
Application granted granted Critical
Publication of DE602004006831T2 publication Critical patent/DE602004006831T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE602004006831T 2003-01-09 2004-01-08 Reinigung von polypeptiden Expired - Lifetime DE602004006831T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43941803P 2003-01-09 2003-01-09
US439418P 2003-01-09
PCT/US2004/000499 WO2004092393A1 (en) 2003-01-09 2004-01-08 Purification of polypeptides

Publications (2)

Publication Number Publication Date
DE602004006831D1 DE602004006831D1 (de) 2007-07-19
DE602004006831T2 true DE602004006831T2 (de) 2008-02-14

Family

ID=33299572

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004006831T Expired - Lifetime DE602004006831T2 (de) 2003-01-09 2004-01-08 Reinigung von polypeptiden

Country Status (18)

Country Link
US (2) US7169908B2 (https=)
EP (1) EP1581644B1 (https=)
JP (1) JP2006517415A (https=)
KR (1) KR20050095605A (https=)
CN (1) CN100467611C (https=)
AT (1) ATE364092T1 (https=)
AU (1) AU2004230670B2 (https=)
BR (1) BRPI0406470A (https=)
CA (1) CA2511946A1 (https=)
DE (1) DE602004006831T2 (https=)
DK (1) DK1581644T3 (https=)
ES (1) ES2287687T3 (https=)
MX (1) MXPA05007378A (https=)
NZ (1) NZ540895A (https=)
PL (1) PL377653A1 (https=)
RU (1) RU2337968C2 (https=)
WO (1) WO2004092393A1 (https=)
ZA (1) ZA200504990B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011001743A1 (de) * 2011-04-01 2012-10-04 Technische Universität Dortmund Verfahren zur Trennung/Reinigung von Biomolekühlen

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2245114T3 (es) 1998-08-06 2005-12-16 Mountain View Pharmaceuticals, Inc. Conjugados de peg-oxidasa de urato y su uso.
US6967241B2 (en) * 2002-09-06 2005-11-22 Genentech, Inc. Process for protein extraction
HUE052976T2 (hu) 2005-04-11 2021-06-28 Horizon Pharma Rheumatology Llc Urát-oxidáz variáns formái és azok alkalmazása
TWI418564B (zh) * 2005-04-11 2013-12-11 Savient Pharmaceuticals Inc 利用陽離子界面活性劑之蛋白質純化
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
CN101622270B (zh) * 2006-04-12 2014-01-01 萨文特医药公司 用阳离子表面活性剂纯化蛋白质的方法
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
CN101611145B (zh) * 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
NZ577933A (en) 2007-01-22 2011-12-22 Genentech Inc Polyelectrolyte precipitation and purification of antibodies
KR20190140090A (ko) 2007-07-09 2019-12-18 제넨테크, 인크. 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지
US7887821B2 (en) * 2007-12-20 2011-02-15 Alk-Abello A/S Process for producing an allergen extract
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
GB0818228D0 (en) * 2008-10-06 2008-11-12 Avecia Biolog Ltd Purification process
EP2337787A1 (en) * 2008-10-17 2011-06-29 Percivia LLC. Clarification process
CN102257122B (zh) 2008-12-16 2015-07-29 Emd密理博公司 搅拌槽反应器及方法
HUP1200205A3 (en) 2009-06-25 2012-09-28 Savient Pharmaceuticals Method and kits for perdicting infusion reaction risk and antibody-mediated low of response by monitoring serum uric acid during pegylated uricare therapy
CA2788607C (en) * 2010-02-01 2018-03-20 Digna Biotech,S.L. Process for the production of interferon alpha 5
US9884111B2 (en) * 2010-02-12 2018-02-06 Laboratorios Leti, S.L. Process for producing an allergen extract
US9884904B2 (en) * 2010-03-10 2018-02-06 Hoffmann-La Roche Inc. Method for purifying polypeptide solutions
JO3131B1 (ar) 2010-04-27 2017-09-20 Glaxosmithkline Llc مركبات كيميائية
CN107312062B (zh) 2010-05-17 2021-03-16 Emd密理博公司 用于纯化生物分子的刺激响应性聚合物
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
CN107266577B (zh) * 2011-10-11 2022-09-13 弗·哈夫曼-拉罗切有限公司 双特异性抗体的改进的组装
CN102443055B (zh) * 2011-10-26 2017-07-28 浙江海正药业股份有限公司 一种重组人肿瘤坏死因子相关凋亡诱导配体的纯化工艺
SG11201407801VA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations
CN102701501A (zh) * 2012-06-26 2012-10-03 天津市津华盛生物科技有限公司 工业乳酸链球菌素废水综合利用的方法
KR20150118103A (ko) * 2013-02-06 2015-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 정제 방법
SG11201505203XA (en) * 2013-02-26 2015-08-28 Agency Science Tech & Res Protein purification in the presence of nonionic organic polymers and electropositive surfaces
JP2016509068A (ja) * 2013-02-28 2016-03-24 エイジェンシー・フォー・サイエンス,テクノロジー・アンド・リサーチ クロマチン欠損細胞培養採取物からの抗体のクロマトグラフィー精製
CN105008384A (zh) 2013-02-28 2015-10-28 新加坡科技研究局 在高电导率下在非离子性有机聚合物的存在下的蛋白质纯化
RU2646098C2 (ru) * 2013-04-30 2018-03-01 Интас Фармасьютикалс Лтд Новый способ клонирования, экспрессии и очистки для получения ранибизумаба
WO2015166037A1 (en) * 2014-04-30 2015-11-05 Novozymes A/S Method for reducing the dna content of a fermentation broth
CN110234340A (zh) 2016-11-11 2019-09-13 好利恩风湿病制药有限责任公司 泼尼松和尿酸酶分子的组合疗法及其用途
US20200237881A1 (en) 2019-01-30 2020-07-30 Horizon Pharma Rheumatology Llc Reducing immunogenicity to pegloticase
CN108333263A (zh) * 2017-01-20 2018-07-27 北京蛋白质组研究中心 一种尿蛋白制备方法及尿蛋白质组的检测方法
TW201927335A (zh) * 2017-10-02 2019-07-16 美商拜耳保健有限責任公司 防止雙硫鍵於細胞培養收集物中還原之方法
BR112020010615A2 (pt) * 2017-12-22 2020-10-27 Regeneron Pharmaceuticals, Inc. produto farmacêutico proteico, método e sistema para caracterizar impurezas de produto farmacêutico proteico e de fármaco de baixo peso molecular, método para produzir um anticorpo, anticorpo, e, usos do método e do sistema
CN111363024A (zh) * 2018-12-26 2020-07-03 深圳翰宇药业股份有限公司 多肽的纯化方法
WO2020247983A1 (en) * 2019-06-05 2020-12-10 Viome, Inc. Sample collection methods and devices
EP4632063A3 (en) 2020-04-15 2025-10-22 Genentech, Inc. Copper loss mitigation
CN112708566A (zh) * 2021-01-25 2021-04-27 西南林业大学 一种黑曲霉生产胞外多聚物的优化培养基及培养方法
CN114774500A (zh) * 2022-04-26 2022-07-22 成都蜀星饲料有限公司 多肽及制备方法、有机复合微量元素及制备方法
US12269875B2 (en) 2023-08-03 2025-04-08 Jeff R. Peterson Gout flare prevention methods using IL-1BETA blockers

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL34079A (en) * 1969-04-01 1973-02-28 Upjohn Co Purification of ypsilon-globulins
US3607857A (en) * 1970-03-03 1971-09-21 Upjohn Co Process of removing acrinol from gamma globulin using siliceous material such as silica gel
US3903254A (en) * 1971-07-23 1975-09-02 Upjohn Co Separation of erythrocyte stroma from lysing medium and hemoglobin with acrinol
DE2308013A1 (de) * 1973-02-17 1974-09-12 Behringwerke Ag Katalasearme glucoseoxidase und verfahren zu ihrer gewinnung
JPS519035B2 (https=) * 1973-10-31 1976-03-23
SU944580A1 (ru) 1980-02-07 1982-07-23 Кировский научно-исследовательский институт переливания крови Способ получени поверхностного антигена гепатита В
CA1237998A (en) * 1984-04-14 1988-06-14 Akihiro Ginnaga Method for purification of filamentous hemagglutinin
JPS60258127A (ja) * 1984-06-04 1985-12-20 Green Cross Corp:The B型肝炎ワクチンの製造方法
EP0226639B1 (en) * 1985-05-29 1990-11-14 The Green Cross Corporation Process for preparing heterogenic protein
EP0227833B1 (en) * 1985-07-16 1991-01-16 Green Cross Corporation Process for preparing hetero-protein
DE3604947A1 (de) 1986-02-17 1987-08-20 Biotest Pharma Gmbh Verfahren zur herstellung eines immunglobulinhaltigen praeparates und dessen verwendung zur prophylaxe und therapie von aids
FR2600078B1 (fr) 1986-06-12 1989-07-13 Merieux Inst Procede de preparation de facteur xiii a partir du placenta
DE3929504A1 (de) * 1989-09-06 1991-03-07 Behringwerke Ag Verfahren zur reinigung von plasminogen-aktivator-inhibitor 2 (pai-2)
DE4030264A1 (de) * 1990-09-25 1992-04-23 Hoechst Ag Verfahren zur herstellung gereinigter glycolipide durch membrantrennverfahren
GB9215540D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5861263A (en) * 1992-10-20 1999-01-19 Universidad Autonoma De Nuevo Leon Preparation of preserved entamoeba histolytica antigens without enzymatic inhibitors and their use in immunological methods
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5760189A (en) 1995-06-02 1998-06-02 Genetics Institute, Inc. Protein recovery & purification methods
US5714583A (en) 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
CA2289665C (en) 1997-06-13 2005-08-09 Genentech, Inc. Protein recovery by chromatography followed by filtration upon a charged layer
JPH11341947A (ja) * 1998-06-02 1999-12-14 Yakult Honsha Co Ltd 発酵管理方法
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
ATE316097T1 (de) * 2000-01-20 2006-02-15 Univ Minnesota Peptide mit antibakterieller wirkung

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011001743A1 (de) * 2011-04-01 2012-10-04 Technische Universität Dortmund Verfahren zur Trennung/Reinigung von Biomolekühlen

Also Published As

Publication number Publication date
ZA200504990B (en) 2006-08-30
BRPI0406470A (pt) 2005-12-06
JP2006517415A (ja) 2006-07-27
DK1581644T3 (da) 2007-10-08
EP1581644B1 (en) 2007-06-06
US7169908B2 (en) 2007-01-30
ES2287687T3 (es) 2007-12-16
EP1581644A1 (en) 2005-10-05
AU2004230670B2 (en) 2010-11-25
CA2511946A1 (en) 2004-10-28
ATE364092T1 (de) 2007-06-15
DE602004006831D1 (de) 2007-07-19
WO2004092393A1 (en) 2004-10-28
US7579448B2 (en) 2009-08-25
MXPA05007378A (es) 2005-11-23
US20070077625A1 (en) 2007-04-05
AU2004230670A1 (en) 2004-10-28
KR20050095605A (ko) 2005-09-29
US20050037456A1 (en) 2005-02-17
RU2005125210A (ru) 2006-01-10
PL377653A1 (pl) 2006-02-06
CN100467611C (zh) 2009-03-11
CN1759186A (zh) 2006-04-12
NZ540895A (en) 2007-03-30
RU2337968C2 (ru) 2008-11-10

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