KR20010012175A - 폴리뉴클레오티드의 2단계 혼성화 및 포획법 - Google Patents
폴리뉴클레오티드의 2단계 혼성화 및 포획법 Download PDFInfo
- Publication number
- KR20010012175A KR20010012175A KR19997010124A KR19997010124A KR20010012175A KR 20010012175 A KR20010012175 A KR 20010012175A KR 19997010124 A KR19997010124 A KR 19997010124A KR 19997010124 A KR19997010124 A KR 19997010124A KR 20010012175 A KR20010012175 A KR 20010012175A
- Authority
- KR
- South Korea
- Prior art keywords
- probe
- target polynucleotide
- capture
- hybridization
- capture probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 227
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 227
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 227
- 238000009396 hybridization Methods 0.000 title claims abstract description 175
- 239000000523 sample Substances 0.000 claims abstract description 474
- 238000000034 method Methods 0.000 claims abstract description 106
- 150000007523 nucleic acids Chemical class 0.000 claims description 105
- 102000039446 nucleic acids Human genes 0.000 claims description 96
- 108020004707 nucleic acids Proteins 0.000 claims description 96
- 230000000295 complement effect Effects 0.000 claims description 70
- 125000003729 nucleotide group Chemical group 0.000 claims description 65
- 239000002773 nucleotide Substances 0.000 claims description 64
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 61
- 230000003321 amplification Effects 0.000 claims description 60
- 230000027455 binding Effects 0.000 claims description 59
- 108091034117 Oligonucleotide Proteins 0.000 claims description 49
- 238000006243 chemical reaction Methods 0.000 claims description 40
- 239000000203 mixture Substances 0.000 claims description 19
- 229910052739 hydrogen Inorganic materials 0.000 claims description 18
- 239000001257 hydrogen Substances 0.000 claims description 18
- 229920001519 homopolymer Polymers 0.000 claims description 16
- 238000013518 transcription Methods 0.000 claims description 15
- 230000035897 transcription Effects 0.000 claims description 15
- 108091093037 Peptide nucleic acid Proteins 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 7
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 7
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 7
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims description 6
- 229930010555 Inosine Natural products 0.000 claims description 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 6
- 229960003786 inosine Drugs 0.000 claims description 6
- 108091028664 Ribonucleotide Proteins 0.000 claims description 5
- 230000001149 cognitive effect Effects 0.000 claims description 5
- 239000002336 ribonucleotide Substances 0.000 claims description 5
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 4
- 229960000643 adenine Drugs 0.000 claims description 4
- 229940104302 cytosine Drugs 0.000 claims description 4
- 229940113082 thymine Drugs 0.000 claims description 4
- 229930024421 Adenine Natural products 0.000 claims description 3
- 125000001475 halogen functional group Chemical group 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229940035893 uracil Drugs 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 abstract description 43
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 33
- 238000003556 assay Methods 0.000 description 33
- 239000000243 solution Substances 0.000 description 31
- 239000013615 primer Substances 0.000 description 27
- 239000002253 acid Substances 0.000 description 19
- 150000007513 acids Chemical class 0.000 description 19
- 239000011324 bead Substances 0.000 description 19
- 238000001514 detection method Methods 0.000 description 18
- 241000606153 Chlamydia trachomatis Species 0.000 description 17
- 229940038705 chlamydia trachomatis Drugs 0.000 description 17
- 125000005647 linker group Chemical group 0.000 description 17
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 14
- 239000000872 buffer Substances 0.000 description 13
- 201000008827 tuberculosis Diseases 0.000 description 13
- 238000003752 polymerase chain reaction Methods 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 11
- 210000002700 urine Anatomy 0.000 description 10
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 9
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 9
- 102100034343 Integrase Human genes 0.000 description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 239000013642 negative control Substances 0.000 description 9
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 9
- 230000003252 repetitive effect Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 6
- 239000006249 magnetic particle Substances 0.000 description 6
- 238000010369 molecular cloning Methods 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 5
- 241000588653 Neisseria Species 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 5
- 229940043264 dodecyl sulfate Drugs 0.000 description 5
- 238000007834 ligase chain reaction Methods 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000006116 polymerization reaction Methods 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 101710203526 Integrase Proteins 0.000 description 4
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 206010018612 Gonorrhoea Diseases 0.000 description 3
- 239000007995 HEPES buffer Substances 0.000 description 3
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 3
- 101710137500 T7 RNA polymerase Proteins 0.000 description 3
- 208000022362 bacterial infectious disease Diseases 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- 241000713869 Moloney murine leukemia virus Species 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000007630 basic procedure Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 230000009918 complex formation Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 208000001786 gonorrhea Diseases 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- WAHQBNXSPALNEA-UHFFFAOYSA-L lithium succinate Chemical compound [Li+].[Li+].[O-]C(=O)CCC([O-])=O WAHQBNXSPALNEA-UHFFFAOYSA-L 0.000 description 2
- 229960004254 lithium succinate Drugs 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002987 primer (paints) Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 2
- MGAXHFMCFLLMNG-UHFFFAOYSA-N 1h-pyrimidine-6-thione Chemical compound SC1=CC=NC=N1 MGAXHFMCFLLMNG-UHFFFAOYSA-N 0.000 description 1
- PIINGYXNCHTJTF-UHFFFAOYSA-N 2-(2-azaniumylethylamino)acetate Chemical group NCCNCC(O)=O PIINGYXNCHTJTF-UHFFFAOYSA-N 0.000 description 1
- XZLIYCQRASOFQM-UHFFFAOYSA-N 5h-imidazo[4,5-d]triazine Chemical compound N1=NC=C2NC=NC2=N1 XZLIYCQRASOFQM-UHFFFAOYSA-N 0.000 description 1
- BXJHWYVXLGLDMZ-UHFFFAOYSA-N 6-O-methylguanine Chemical compound COC1=NC(N)=NC2=C1NC=N2 BXJHWYVXLGLDMZ-UHFFFAOYSA-N 0.000 description 1
- ZAOGIVYOCDXEAK-UHFFFAOYSA-N 6-n-methyl-7h-purine-2,6-diamine Chemical compound CNC1=NC(N)=NC2=C1NC=N2 ZAOGIVYOCDXEAK-UHFFFAOYSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 101900234631 Escherichia coli DNA polymerase I Proteins 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 241000187489 Mycobacterium simiae Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 241001302239 Mycobacterium tuberculosis complex Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 description 1
- 108091028733 RNTP Proteins 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 125000003545 alkoxy group Chemical group 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 description 1
- 239000012148 binding buffer Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000006297 carbonyl amino group Chemical group [H]N([*:2])C([*:1])=O 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 108091092330 cytoplasmic RNA Proteins 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000021550 forms of sugar Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 235000014304 histidine Nutrition 0.000 description 1
- 150000002411 histidines Chemical class 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 201000001371 inclusion conjunctivitis Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical group 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- OYRRZWATULMEPF-UHFFFAOYSA-N pyrimidin-4-amine Chemical compound NC1=CC=NC=N1 OYRRZWATULMEPF-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 239000006104 solid solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 206010044325 trachoma Diseases 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Electrical Control Of Air Or Fuel Supplied To Internal-Combustion Engine (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4543097P | 1997-05-02 | 1997-05-02 | |
| US60/045,430 | 1997-05-02 | ||
| PCT/US1998/008853 WO1998050583A1 (en) | 1997-05-02 | 1998-05-01 | Two-step hybridization and capture of a polynucleotide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20010012175A true KR20010012175A (ko) | 2001-02-15 |
Family
ID=21937832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR19997010124A Withdrawn KR20010012175A (ko) | 1997-05-02 | 1998-05-01 | 폴리뉴클레오티드의 2단계 혼성화 및 포획법 |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US6110678A (enExample) |
| EP (1) | EP0975807B1 (enExample) |
| JP (1) | JP4222635B2 (enExample) |
| KR (1) | KR20010012175A (enExample) |
| AT (1) | ATE340868T1 (enExample) |
| AU (1) | AU738708B2 (enExample) |
| CA (1) | CA2287570C (enExample) |
| DE (1) | DE69836012T2 (enExample) |
| DK (1) | DK0975807T3 (enExample) |
| ES (1) | ES2271994T3 (enExample) |
| WO (1) | WO1998050583A1 (enExample) |
Families Citing this family (213)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| US7622294B2 (en) * | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US20030027126A1 (en) | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| ES2324503T3 (es) | 1997-04-10 | 2009-08-07 | Stichting Katholieke Universiteit University Medical Centre Nijmegen | Pca3, genes pca3 y metodos de uso. |
| DK0975807T3 (da) * | 1997-05-02 | 2007-01-29 | Gen Probe Inc | To-trins hybridisering og indfangning af et polynukleotid |
| US6534273B2 (en) | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| US6849400B1 (en) | 1997-07-23 | 2005-02-01 | Gen-Probe Incorporated | Methods for detecting and measuring spliced nucleic acids |
| US7115884B1 (en) | 1997-10-06 | 2006-10-03 | Trustees Of Tufts College | Self-encoding fiber optic sensor |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| WO1999055365A1 (en) | 1998-04-30 | 1999-11-04 | Cornell Research Foundation, Inc. | Adenoviral vectors with tandem fiber proteins |
| EP2316571A3 (en) | 1998-05-01 | 2011-07-27 | Gen-Probe Incorporated | Automated diagnostic analyzer and method |
| US8337753B2 (en) | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
| WO1999067641A2 (en) | 1998-06-24 | 1999-12-29 | Illumina, Inc. | Decoding of array sensors with microspheres |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| US6544732B1 (en) * | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| EP1190100B1 (en) * | 1999-05-20 | 2012-07-25 | Illumina, Inc. | Combinatorial decoding of random nucleic acid arrays |
| US8080380B2 (en) | 1999-05-21 | 2011-12-20 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US8481268B2 (en) | 1999-05-21 | 2013-07-09 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| EP1187939B9 (en) * | 1999-07-09 | 2009-08-12 | Gen-Probe Incorporated | Detection of hiv-1 by nucleic acid amplification |
| WO2001012862A2 (en) | 1999-08-18 | 2001-02-22 | Illumina, Inc. | Compositions and methods for preparing oligonucleotide solutions |
| JP2003510075A (ja) * | 1999-09-29 | 2003-03-18 | ディグノキュアー・インコーポレーテッド | 良性および悪性の前立腺組織におけるｐｃａ３メッセンジャーｒｎａ種 |
| US6428957B1 (en) * | 1999-11-08 | 2002-08-06 | Agilent Technologies, Inc. | Systems tools and methods of assaying biological materials using spatially-addressable arrays |
| US6747141B2 (en) | 1999-12-15 | 2004-06-08 | Gen-Probe Incorporated | Methods and compositions for detection of mycobacterium avium complex species |
| US6664081B2 (en) * | 1999-12-17 | 2003-12-16 | Gen-Probe Incorporated | Nucleic acid amplification and detection of mycobacterium species |
| CA2389523C (en) * | 1999-12-17 | 2012-03-06 | Gen-Probe Incorporated | Nucleic acid amplification and detection of mycobacterium xenopi 16s ribosomal rna (rrna) or dna encoding 16s rrna in a biological sample |
| US7361488B2 (en) * | 2000-02-07 | 2008-04-22 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US6913884B2 (en) * | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
| EP1259643B1 (en) * | 2000-02-07 | 2008-10-15 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
| US8076063B2 (en) | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
| AU3806701A (en) | 2000-02-07 | 2001-08-14 | Illumina Inc | Nucleic acid detection methods using universal priming |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| CA2399908A1 (en) * | 2000-02-10 | 2001-08-16 | Todd Dickinson | Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor |
| WO2001061043A2 (en) | 2000-02-16 | 2001-08-23 | Illumina, Inc. | Parallel genotyping of multiple patient samples |
| AU2001247414A1 (en) | 2000-03-14 | 2001-09-24 | Active Motif | Oligonucleotide analogues, methods of synthesis and methods of use |
| US20040014644A1 (en) * | 2000-03-14 | 2004-01-22 | Vladimir Efimov | Oligonucleotide analogues and methods of use for modulating gene expression |
| US6962906B2 (en) * | 2000-03-14 | 2005-11-08 | Active Motif | Oligonucleotide analogues, methods of synthesis and methods of use |
| US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US6582920B2 (en) | 2000-09-01 | 2003-06-24 | Gen-Probe Incorporated | Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations |
| JP4965793B2 (ja) | 2000-09-01 | 2012-07-04 | ジェン−プローブ・インコーポレーテッド | 薬剤耐性突然変異と関連する配列検出のためのｈｉｖ−１配列の増幅 |
| US6870045B2 (en) | 2000-10-23 | 2005-03-22 | Gen-Probe Incorporated | Kits for detecting HIV-2 |
| US20040018491A1 (en) * | 2000-10-26 | 2004-01-29 | Kevin Gunderson | Detection of nucleic acid reactions on bead arrays |
| DE10126630A1 (de) * | 2001-05-31 | 2003-01-09 | Peter Und Traudl Engelhorn Sti | Verfahren zur Zellsortierung |
| US7094541B2 (en) | 2001-08-31 | 2006-08-22 | Gen-Probe Incorporated | Assay for detection of human parvovirus B19 nucleic acid |
| WO2003031934A2 (en) * | 2001-10-09 | 2003-04-17 | Chiron Corporation | Identification of oligonucleotides for the capture, detection and quantitation of hepatitis b viral dna |
| JP2005517900A (ja) * | 2001-11-21 | 2005-06-16 | アプレラ コーポレイション | デジタルアッセイ |
| US20030175709A1 (en) * | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| WO2003069333A1 (en) | 2002-02-14 | 2003-08-21 | Illumina, Inc. | Automated information processing in randomly ordered arrays |
| WO2003071252A2 (en) | 2002-02-15 | 2003-08-28 | Exact Sciences Corporation | Methods for analysis of molecular events |
| DE10211321A1 (de) * | 2002-03-14 | 2003-09-25 | Gnothis Holding Sa Ecublens | Verwendung von Abfangsonden beim Nachweis von Nukleinsäuren |
| US7211382B2 (en) * | 2002-04-09 | 2007-05-01 | Orchid Cellmark Inc. | Primer extension using modified nucleotides |
| JP2006501817A (ja) * | 2002-06-03 | 2006-01-19 | パムジーン ビー．ブイ． | 新規高密度アレイおよび検体分析方法 |
| KR20050010902A (ko) * | 2002-06-12 | 2005-01-28 | 카이론 코포레이션 | Ａ형 간염 바이러스 핵산의 포획, 검출 및 정량을 위한올리고뉴클레오티드의 동정방법 |
| EP2292801B1 (en) * | 2002-06-14 | 2018-08-08 | Gen-Probe Incorporated | Compositions and methods for detecting hepatitis b virus |
| EP2977470B1 (en) | 2002-10-16 | 2018-01-03 | Gen-Probe Incorporated | Compositions and methods for detecting west nile virus |
| GB2395557A (en) * | 2002-11-22 | 2004-05-26 | Dynal Biotech Ltd | Nucleic acid probes |
| JP4824540B2 (ja) | 2003-02-07 | 2011-11-30 | ダイアノキュアー インク． | サンプル中の前立腺癌を検出する方法 |
| US6943768B2 (en) | 2003-02-21 | 2005-09-13 | Xtellus Inc. | Thermal control system for liquid crystal cell |
| AU2004276722B2 (en) | 2003-05-19 | 2009-03-12 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample |
| CA2432365A1 (en) * | 2003-06-30 | 2004-12-30 | Jack A. Schalken | Specific method of prostate cancer detection based on pca3, and kits therefore |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| EP2251442B9 (en) | 2003-12-19 | 2012-04-25 | Gen-Probe Incorporated | Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2 |
| US20060266136A1 (en) * | 2004-06-08 | 2006-11-30 | Gnothis Holding Ag | Bi-fluorophore marked probes for detecting nucleic acids |
| JP5020818B2 (ja) * | 2004-07-01 | 2012-09-05 | ジェン−プローブ・インコーポレーテッド | 生物学的試料中の核酸を検出するための方法及び組成物 |
| US7544792B2 (en) | 2004-07-13 | 2009-06-09 | Gen-Probe Incorporated | Compositions and methods for detection of hepatitis A virus nucleic acid |
| US7662562B2 (en) * | 2004-08-10 | 2010-02-16 | Becton, Dickinson And Company | Method for rapid identification of microorganisms |
| WO2006026654A2 (en) * | 2004-08-27 | 2006-03-09 | Exact Sciences Corporation | Method for detecting a recombinant event |
| CA2929741A1 (en) | 2004-09-30 | 2006-04-13 | Gen-Probe Incorporated | Assay for detecting and quantifying hiv-1 |
| US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
| CA2491067A1 (en) | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
| DE602006019203D1 (de) | 2005-02-18 | 2011-02-10 | Gen Probe Inc | Probenherstellungsverfahren mit einem alkali-stoss |
| US7964413B2 (en) | 2005-03-10 | 2011-06-21 | Gen-Probe Incorporated | Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay |
| US20060257902A1 (en) * | 2005-03-25 | 2006-11-16 | Ambion, Inc. | Methods and compositions for depleting abundant RNA transcripts |
| US9777314B2 (en) | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
| ATE551433T1 (de) * | 2005-05-06 | 2012-04-15 | Gen Probe Inc | Verfahren und produkte zum erfassen von nukleinsäurezielen |
| AU2006244460B2 (en) | 2005-05-06 | 2010-04-08 | Gen-Probe Incorporated | Compositions and assays to detect influenza virus A and B nucleic acids |
| WO2006133385A2 (en) * | 2005-06-06 | 2006-12-14 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of chlamydophila pneumoniae in a test sample |
| CA2625414A1 (en) | 2005-10-17 | 2007-04-26 | Gen-Probe Incorporated | Compositions and methods to detect legionella pneumophila nucleic acid |
| EP2479291A1 (en) * | 2005-10-27 | 2012-07-25 | Life Technologies Corporation | Nucleic acid amplification using non-random primers |
| EP1957645B1 (en) | 2005-12-06 | 2010-11-17 | Ambion Inc. | Reverse transcription primers and methods of design |
| AU2007249286B2 (en) * | 2006-05-12 | 2013-06-13 | Gen-Probe Incorporated | Compositions and methods to detect enterococci nucleic acid |
| AU2007267517B2 (en) * | 2006-05-24 | 2012-12-20 | Gen-Probe Incorporated | Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe |
| AU2013201413B2 (en) * | 2006-05-24 | 2014-05-29 | Gen-Probe Incorporated | Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe |
| AU2007258455B2 (en) | 2006-06-06 | 2013-05-02 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| WO2008016988A1 (en) | 2006-08-01 | 2008-02-07 | Gen-Probe Incorporated | Methods of nonspecific target capture of nucleic acids |
| WO2008080029A2 (en) | 2006-12-21 | 2008-07-03 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| EP1978111B1 (en) | 2007-04-02 | 2013-03-27 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa |
| EP2067867A1 (en) * | 2007-12-03 | 2009-06-10 | Siemens Aktiengesellschaft | Process for concentrating nucleic acid molecules |
| AU2008345600B2 (en) | 2007-12-21 | 2014-07-24 | Gen-Probe Incorporated | Detection of antibiotic-resistant microorganisms |
| EP3392351A1 (en) | 2008-04-21 | 2018-10-24 | Gen-Probe Incorporated | Method for detecting chikungunya virus |
| EP2288726B1 (en) | 2008-05-13 | 2012-06-13 | Gen-Probe Incorporated | Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences |
| JP2011521651A (ja) | 2008-05-30 | 2011-07-28 | ジェン−プロウブ インコーポレイテッド | サルモネラの検出および／またはモニタリングのための組成物、キットおよび関連方法 |
| JP2012508586A (ja) * | 2008-11-14 | 2012-04-12 | ジェン−プローブ・インコーポレーテッド | カンピロバクター属（Ｃａｍｐｙｌｏｂａｃｔｅｒ）核酸を検出するための組成物、キットおよび方法 |
| EP2367958B1 (en) * | 2008-11-25 | 2017-08-23 | Gen-Probe Incorporated | Compositions and methods for detecting small rnas, and uses thereof |
| AU2009324806A1 (en) * | 2008-12-10 | 2011-07-07 | Smiths Detection Inc. | Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization |
| WO2010078374A1 (en) | 2008-12-30 | 2010-07-08 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of listeria |
| EP3257859B1 (en) | 2009-02-26 | 2020-09-23 | Gen-Probe Incorporated | Assay for detection of human parvovirus nucleic acid |
| EP2829616B1 (en) | 2009-06-23 | 2018-09-26 | Gen-Probe Incorporated | Composition and methods for detecting nucleic acid from mollicutes |
| EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| CA2770888C (en) | 2009-08-31 | 2020-04-14 | Gen-Probe Incorporated | Dengue virus assay |
| EP2531515B1 (en) | 2010-01-22 | 2015-10-21 | Gen-Probe Incorporated | Probes for detecting the presence of trochomonas vaginalis in a sample |
| EP2536849B1 (en) | 2010-02-17 | 2016-05-11 | Gen-Probe Incorporated | Compositions and methods to detect atopobium vaginae nucleic acid |
| US9206484B2 (en) | 2010-04-21 | 2015-12-08 | Gen-Probe Incorporated | Compositions, methods and kits to detect herpes simplex virus nucleic acids |
| US9234249B2 (en) | 2010-07-12 | 2016-01-12 | Gen-Probe Incorporated | Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids |
| EP2754496A3 (en) | 2010-07-23 | 2017-08-02 | Beckman Coulter, Inc. | System Or Method Of Including Analytical Units |
| US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
| WO2012030856A2 (en) | 2010-08-30 | 2012-03-08 | Gen-Probe Incorporated | Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus |
| EP2616555B1 (en) | 2010-09-16 | 2017-11-08 | Gen-Probe Incorporated | Capture probes immobilizable via l-nucleotide tail |
| WO2012046219A2 (en) | 2010-10-04 | 2012-04-12 | Gen-Probe Prodesse, Inc. | Compositions, methods and kits to detect adenovirus nucleic acids |
| US9255293B2 (en) | 2010-11-01 | 2016-02-09 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| WO2012116308A1 (en) | 2011-02-24 | 2012-08-30 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| AU2012236109A1 (en) * | 2011-04-01 | 2013-11-21 | Occam Biolabs, Inc. | Methods and kits for detecting cell-free pathogen-specific nucleic acids |
| EP3604559A1 (en) | 2011-04-25 | 2020-02-05 | Gen-Probe Incorporated | Compositions and methods for detecting bv-associated bacterial nucleic acid |
| EP2732054B1 (en) | 2011-07-15 | 2019-04-03 | Gen-Probe Incorporated | Method for detecting human parvovirus nucleic acid in single-plex or multiplex assays |
| EP3620533B1 (en) | 2011-09-06 | 2023-01-18 | Gen-Probe Incorporated | Closed nucleic acid structures |
| EP3255155B1 (en) | 2011-09-08 | 2019-04-24 | Gen-Probe Incorporated | Compositions and methods for detecting bv-associated bacterial nucleic acid |
| AU2012312169B2 (en) | 2011-09-21 | 2016-01-14 | Gen-Probe Incorporated | Methods for amplifying nucleic acid using tag-mediated displacement |
| WO2013049135A1 (en) | 2011-09-26 | 2013-04-04 | Gen-Probe Incorporated | Algorithms for sequence determinations |
| EP3373015A1 (en) | 2011-11-07 | 2018-09-12 | Beckman Coulter Inc. | Aliquotter system and workflow |
| BR112014010955A2 (pt) | 2011-11-07 | 2017-06-06 | Beckman Coulter Inc | sistema e método para processar amostras |
| KR20140091033A (ko) | 2011-11-07 | 2014-07-18 | 베크만 컬터, 인코포레이티드 | 검체 컨테이너 검출 |
| ES2729283T3 (es) | 2011-11-07 | 2019-10-31 | Beckman Coulter Inc | Sistema de centrífuga y flujo de trabajo |
| BR112014011048A2 (pt) | 2011-11-07 | 2017-05-02 | Beckman Coulter Inc | braço robótico |
| EP2776847A1 (en) | 2011-11-07 | 2014-09-17 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
| DE102011120550B4 (de) | 2011-12-05 | 2013-11-07 | Gen-Probe Prodesse, Inc. | Zusammensetzungen, Verfahren und Kits zur Detektion von Adenovirusnukleinsäuren |
| EP2809807B1 (en) | 2012-02-01 | 2019-04-10 | Gen-Probe Incorporated | Asymmetric hairpin target capture oligomers |
| WO2013126793A2 (en) | 2012-02-24 | 2013-08-29 | Gen-Probe Prodesse, Inc. | Detection of shiga toxin genes in bacteria |
| EP2824180B1 (en) * | 2012-03-05 | 2019-04-03 | NGK Insulators, Ltd. | Method for detecting target nucleic acid |
| AU2013205110B2 (en) | 2012-04-24 | 2016-10-13 | Gen-Probe Incorporated | Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids |
| AU2013205064B2 (en) | 2012-06-04 | 2015-07-30 | Gen-Probe Incorporated | Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid |
| AU2013205087B2 (en) | 2012-07-13 | 2016-03-03 | Gen-Probe Incorporated | Method for detecting a minority genotype |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) * | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3640348A1 (en) | 2012-08-30 | 2020-04-22 | Gen-Probe Incorporated | Multiphase nucleic acid amplification |
| AU2013205122B2 (en) | 2012-10-11 | 2016-11-10 | Gen-Probe Incorporated | Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid |
| AU2013205090B2 (en) | 2012-12-07 | 2016-07-28 | Gen-Probe Incorporated | Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| BR112015019159A2 (pt) | 2013-02-08 | 2017-07-18 | 10X Genomics Inc | geração de código de barras de polinucleotídeos |
| EP4354149B1 (en) | 2013-03-15 | 2025-07-02 | Abbott Laboratories | Diagnostic analyzers with pretreatment carousels and related methods |
| CN107966576B (zh) | 2013-03-15 | 2021-08-03 | 雅培制药有限公司 | 具有后面可进入轨道系统的自动化诊断分析仪及相关方法 |
| US9400285B2 (en) | 2013-03-15 | 2016-07-26 | Abbot Laboratories | Automated diagnostic analyzers having vertically arranged carousels and related methods |
| WO2015023892A1 (en) | 2013-08-14 | 2015-02-19 | Gen-Probe Incorporated | Compositions and methods for detecting hev nucleic acid |
| WO2015070173A1 (en) * | 2013-11-08 | 2015-05-14 | Isis Pharmaceuticals, Inc. | Compounds and methods for detecting oligonucleotides |
| EP3670663B1 (en) | 2014-02-28 | 2024-08-07 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| DE102015203606C5 (de) | 2014-02-28 | 2023-12-21 | Gen-Probe Incorporated | Verfahren zur Isolierung von Nukleinsäure von zytologischen Proben in flüssigen Konservierungsmitteln, die Formaldehyd enthalten |
| CN106413896B (zh) | 2014-04-10 | 2019-07-05 | 10X基因组学有限公司 | 用于封装和分割试剂的流体装置、系统和方法及其应用 |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| JP2017522866A (ja) | 2014-06-26 | 2017-08-17 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列の分析 |
| GB2555995B (en) | 2014-10-20 | 2019-06-26 | Gen Probe Inc | Red blood cell lysis solution |
| JP6944873B2 (ja) | 2015-01-09 | 2021-10-06 | ジェン−プローブ・インコーポレーテッド | 細菌性膣症を診断するための方法および組成物 |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| AU2016233298B2 (en) | 2015-03-16 | 2021-09-02 | Gen-Probe Incorporated | Methods and compositions for detecting bacterial nucleic acid and diagnosing bacterial vaginosis |
| CN106868098A (zh) * | 2015-12-10 | 2017-06-20 | 益善生物技术股份有限公司 | 循环肿瘤细胞分型鉴定试剂盒 |
| EP3400309B1 (en) | 2016-01-04 | 2025-03-19 | Gen-Probe Incorporated | Methods and compositions for detecting candida species |
| EP3448998B1 (en) | 2016-04-27 | 2020-06-03 | Gen-Probe Incorporated | Blood cell lysis reagent |
| WO2017214511A2 (en) | 2016-06-10 | 2017-12-14 | Gen-Probe Incorporated | Compositions and methods for detecting zika virus nucleic acid |
| EP3485012A4 (en) * | 2016-07-15 | 2020-03-25 | Northwestern University | COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACIDS IN EXPECTORATIONS |
| AU2017325833B2 (en) | 2016-09-15 | 2022-06-16 | Abbott Laboratories | Devices and methods for sample analysis |
| JP7167013B2 (ja) | 2016-10-19 | 2022-11-08 | ジェン－プローブ・インコーポレーテッド | Ｃ型肝炎ウイルスを検出または定量するための組成物および方法 |
| CA3042451A1 (en) | 2016-11-21 | 2018-05-24 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying hepatitis b virus |
| US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| WO2018160979A1 (en) | 2017-03-03 | 2018-09-07 | Gen-Probe Incorporated | Evaporation-limiting inserts for reagent containers and related methods of use |
| WO2018175868A1 (en) | 2017-03-24 | 2018-09-27 | Gen-Probe Incorporated | Compositions and methods for detection of viral pathogens in samples |
| US11384387B2 (en) | 2017-03-25 | 2022-07-12 | Gen-Probe Incorporated | Compositions, methods and kits to detect adenovirus nucleic acid |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| SG11201901822QA (en) | 2017-05-26 | 2019-03-28 | 10X Genomics Inc | Single cell analysis of transposase accessible chromatin |
| EP3635140B1 (en) | 2017-06-07 | 2023-05-31 | Gen-Probe Incorporated | Detecting babesia species nucleic acid in a sample |
| AU2018300000B2 (en) | 2017-07-10 | 2021-11-11 | Gen-Probe Incorporated | Analytical systems and methods for nucleic acid amplification using sample assigning parameters |
| EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019099629A1 (en) | 2017-11-17 | 2019-05-23 | Gen-Probe Incorporated | COMPOSITIONS AND METHODS FOR DETECTING C1orf43 NUCLEIC ACID |
| DK3746225T3 (da) | 2018-01-29 | 2025-01-13 | Gen Probe Inc | Analytiske systemer og fremgangsmåder |
| EP3775271B1 (en) | 2018-04-06 | 2025-03-12 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| CN112654721B (zh) | 2018-06-13 | 2025-01-07 | 简·探针公司 | 用于检测b群链球菌核酸的组合物和方法 |
| CA3103268A1 (en) | 2018-06-28 | 2020-01-02 | Gen-Probe Incorporated | Sample preparation method and system |
| US20210318324A1 (en) * | 2018-07-05 | 2021-10-14 | The Board Of Regents Of The University Of Oklahoma | Gene signatures for cancer characterization and methods of use |
| US20210317515A1 (en) | 2018-07-10 | 2021-10-14 | Gen-Probe Incorporated | Methods and systems for detecting and quantifying nucleic acids |
| JP7469290B2 (ja) | 2018-08-01 | 2024-04-16 | ジェン－プローブ・インコーポレーテッド | エプスタイン－バールウイルスの核酸を検出するための組成物および方法 |
| EP3841223A1 (en) | 2018-08-08 | 2021-06-30 | Gen-Probe Incorporated | Compositions, methods and kits for detecting mycoplasma genitalium |
| AU2019326462B2 (en) | 2018-08-21 | 2025-11-27 | Gen-Probe Incorporated | Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus |
| KR102868953B1 (ko) | 2018-08-24 | 2025-10-13 | 젠-프로브 인코포레이티드 | 세균성 핵산을 검출하고 세균성 질염을 진단하기 위한 조성물 및 방법 |
| EP3856934A2 (en) | 2018-09-27 | 2021-08-04 | Gen-Probe Incorporated | COMPOSITIONS AND METHODS FOR DETECTING BORDETELLA PERTUSSIS AND BORDETELLA
PARAPERTUSSIS NUCLEIC ACID |
| WO2020072409A1 (en) | 2018-10-01 | 2020-04-09 | Gen-Probe Incorporated | Compositions and methods for amplifying or detecting varicella-zoster virus |
| CA3116895A1 (en) | 2018-10-22 | 2020-04-30 | Gen-Probe Incorporated | Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus |
| TW202030333A (zh) | 2018-12-20 | 2020-08-16 | 美商簡 探針公司 | 用於檢測瘧原蟲物種核酸之組成物及方法 |
| AU2020210753A1 (en) | 2019-01-25 | 2021-09-02 | Gen-Probe Incorporated | Detection of drug-resistant mycoplasma genitalium |
| JP7716995B2 (ja) | 2019-07-03 | 2025-08-01 | ジェン－プローブ・インコーポレーテッド | 試料中のＴｒｉｃｈｏｍｏｎａｓ ｖａｇｉｎａｌｉｓの存在を決定する際に使用するためのオリゴヌクレオチド |
| EP4018004B1 (en) | 2019-08-23 | 2025-06-18 | Gen-Probe Incorporated | Compositions, methods and kits for detecting treponema pallidum |
| EP4114985B1 (en) | 2020-03-04 | 2025-05-07 | Gen-Probe Incorporated | Compositions and methods for detecting sars-cov-2 nucleic acid |
| CN115698325A (zh) | 2020-05-07 | 2023-02-03 | 盖立复诊断解决方案公司 | 用于检测SARS-CoV-2核酸的方法和组合物 |
| JP2023534457A (ja) | 2020-07-17 | 2023-08-09 | ジェン－プローブ・インコーポレーテッド | マクロライド耐性Ｍｙｃｏｐｌａｓｍａ ｇｅｎｉｔａｌｉｕｍの検出 |
| WO2022241291A1 (en) | 2021-05-14 | 2022-11-17 | Gen-Probe Incorporated | Compositions and methods for detecting human adenovirus nucleic acid |
| WO2023278529A1 (en) | 2021-07-01 | 2023-01-05 | Gen-Probe Incorporated | Enzyme formulations and reaction mixtures for nucleic acid amplification |
| WO2024054924A1 (en) | 2022-09-08 | 2024-03-14 | Gen-Probe Incorporated | Method of detecting nucleic acid analytes using dual-specificity primers |
| WO2024081776A1 (en) | 2022-10-13 | 2024-04-18 | Gen-Probe Incorporated | Cellularity control for nucleic acid assays |
| AU2023408044A1 (en) | 2022-12-19 | 2025-07-31 | Gen-Probe Incorporated | Compositions and methods for detecting gastrointestinal pathogens |
| WO2024161179A1 (en) | 2023-01-31 | 2024-08-08 | Mobidiag Oy | Compositions and methods for detecting stx nucleic acids |
| AU2024234681A1 (en) | 2023-03-16 | 2025-09-04 | Gen-Probe Incorporated | Compositions and methods for detecting gastrointestinal parasites |
| GB2639519A (en) * | 2023-10-26 | 2025-10-01 | Todd Daniel | Nanites: smart, programmable polymer composite nucleic-acid nanostructures for sensitive, targeted, molecular nucleic acid detection |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| US4554088A (en) | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| GB8401368D0 (en) | 1984-01-19 | 1984-02-22 | Amersham Int Plc | Assay method |
| DE177497T1 (de) | 1984-04-05 | 1986-11-27 | Life Technologies Inc., Gaithersburg, Md. | Immobilisierung von nukleinsaeuren. |
| CA1260372A (en) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US4755458A (en) * | 1984-08-30 | 1988-07-05 | Enzo Biochem, Inc. | Composition and method for the detection of the presence of a polynucleotide sequence of interest |
| US5386022A (en) | 1985-03-28 | 1995-01-31 | Hoffman-La Roche Inc. | Primes and probes for the amplification and detection of aids associated nucleic acids |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5273882A (en) * | 1985-06-13 | 1993-12-28 | Amgen | Method and kit for performing nucleic acid hybridization assays |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US4882269A (en) | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
| CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
| ATE88762T1 (de) * | 1986-08-11 | 1993-05-15 | Siska Diagnostics Inc | Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden. |
| ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| IL86724A (en) * | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| CA1340843C (en) * | 1987-07-31 | 1999-12-07 | J. Lawrence Burg | Selective amplification of target polynucleotide sequences |
| US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
| US5639604A (en) | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| AU2735988A (en) * | 1987-12-21 | 1989-07-13 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| FR2625511B1 (fr) * | 1987-12-31 | 1990-05-18 | Sanofi Sa | Procede d'obtention in vitro de tissus reconstitues |
| JPH03504199A (ja) * | 1988-05-10 | 1991-09-19 | イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー | 迅速な核酸検出法 |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| DE68913555T2 (de) | 1988-11-21 | 1994-07-07 | Dynal As | Sonden aus nukleinsäuren. |
| GB8827160D0 (en) | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
| CA2002076A1 (en) * | 1988-11-21 | 1990-05-21 | Brent A. Burdick | Diagnostic kit and method using a solid phase capture means for detecting nucleic acids |
| US5449769A (en) * | 1989-03-06 | 1995-09-12 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
| ATE118824T1 (de) * | 1989-03-10 | 1995-03-15 | Amoco Corp | Immobilisierte oligonukleotidsonden und ihre verwendungen. |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5112734A (en) * | 1989-05-26 | 1992-05-12 | Gene-Trak Systems | Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna |
| US5656207A (en) * | 1989-06-24 | 1997-08-12 | Gen Probe Incorporated | Detecting or quantifying multiple analytes using labelling techniques |
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| ATE152777T1 (de) * | 1989-12-04 | 1997-05-15 | Microprobe Corp | Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind |
| CA2032203C (en) | 1989-12-29 | 2009-05-19 | Christine L. Brakel | Amplification capture assay |
| US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| EP0529070A1 (en) * | 1991-02-27 | 1993-03-03 | Amoco Corporation | Methods for improving the sensitivity of hybridization assays |
| CA2135073C (en) * | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| KR100316498B1 (ko) * | 1992-08-04 | 2002-06-20 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 | 핵산서열증폭방법 |
| WO1994019023A1 (en) * | 1993-02-19 | 1994-09-01 | Isis Pharmaceuticals, Inc. | Cyclobutyl antisense oligonucleotides, methods of making and use thereof |
| CA2167838C (en) * | 1993-07-23 | 1999-11-23 | Thomas B. Ryder | Methods for enhancing nucleic acid amplification |
| EP0648835A1 (en) * | 1993-10-14 | 1995-04-19 | The Procter & Gamble Company | Use of alkaline polyammonium salts to increase cationic density in fabric softeners |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| DE69535240T2 (de) * | 1994-10-28 | 2007-06-06 | Gen-Probe Inc., San Diego | Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen |
| US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US5731148A (en) * | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
| US6060246A (en) | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
| DK0975807T3 (da) * | 1997-05-02 | 2007-01-29 | Gen Probe Inc | To-trins hybridisering og indfangning af et polynukleotid |
-
1998
- 1998-05-01 DK DK98920106T patent/DK0975807T3/da active
- 1998-05-01 KR KR19997010124A patent/KR20010012175A/ko not_active Withdrawn
- 1998-05-01 DE DE69836012T patent/DE69836012T2/de not_active Expired - Lifetime
- 1998-05-01 EP EP98920106A patent/EP0975807B1/en not_active Expired - Lifetime
- 1998-05-01 ES ES98920106T patent/ES2271994T3/es not_active Expired - Lifetime
- 1998-05-01 AU AU72751/98A patent/AU738708B2/en not_active Expired
- 1998-05-01 CA CA002287570A patent/CA2287570C/en not_active Expired - Lifetime
- 1998-05-01 US US09/070,998 patent/US6110678A/en not_active Expired - Lifetime
- 1998-05-01 WO PCT/US1998/008853 patent/WO1998050583A1/en not_active Ceased
- 1998-05-01 JP JP54824898A patent/JP4222635B2/ja not_active Expired - Lifetime
- 1998-05-01 AT AT98920106T patent/ATE340868T1/de active
-
2000
- 2000-05-19 US US09/574,561 patent/US6280952B1/en not_active Expired - Lifetime
-
2001
- 2001-07-20 US US09/910,635 patent/US20020028459A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US6280952B1 (en) | 2001-08-28 |
| AU738708B2 (en) | 2001-09-27 |
| DE69836012D1 (de) | 2006-11-09 |
| US6110678A (en) | 2000-08-29 |
| ES2271994T3 (es) | 2007-04-16 |
| JP4222635B2 (ja) | 2009-02-12 |
| ATE340868T1 (de) | 2006-10-15 |
| EP0975807A1 (en) | 2000-02-02 |
| EP0975807B1 (en) | 2006-09-27 |
| JP2002511745A (ja) | 2002-04-16 |
| DE69836012T2 (de) | 2007-04-05 |
| WO1998050583A1 (en) | 1998-11-12 |
| CA2287570A1 (en) | 1998-11-12 |
| US20020028459A1 (en) | 2002-03-07 |
| CA2287570C (en) | 2008-10-28 |
| AU7275198A (en) | 1998-11-27 |
| DK0975807T3 (da) | 2007-01-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4222635B2 (ja) | ２段階ハイブリダイゼーションおよびポリヌクレオチドの捕捉 | |
| US6534273B2 (en) | Two-step hybridization and capture of a polynucleotide | |
| EP3095873B1 (en) | Methods and compositions for nucleic acid amplification | |
| US6087133A (en) | Isothermal strand displacement nucleic acid amplification | |
| EP2069536B1 (en) | Methods of nonspecific target capture of nucleic acids | |
| FR2737223A1 (fr) | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres | |
| JPH08205894A (ja) | 核酸の高感度検出方法 | |
| CN110612354A (zh) | 用于分离靶核酸的组合物和方法 | |
| JP4196236B2 (ja) | 核酸増幅用試薬および配列特異的な核酸増幅法 | |
| JP4744053B2 (ja) | マイコバクテリウム属（Ｍｙｃｏｂａｃｔｅｒｉｕｍ）種の核酸増幅および検出 | |
| US7294489B2 (en) | Nucleic acid amplification and detection of Mycobacterium species | |
| CA2389533C (en) | Methods and compositions for detection of mycobacterium avium complex species | |
| JP4455881B2 (ja) | 結核菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍｔｕｂｅｒｃｕｌｏｓｉｓ）のｒｐｏＢ配列の検出 | |
| JP2000500976A (ja) | 多数の二本鎖核酸の生成方法 | |
| JPH05192197A (ja) | 核酸検出法及びキット |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 19991102 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| PC1203 | Withdrawal of no request for examination | ||
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |