DE602006019203D1 - Probenherstellungsverfahren mit einem alkali-stoss - Google Patents

Probenherstellungsverfahren mit einem alkali-stoss

Info

Publication number
DE602006019203D1
DE602006019203D1 DE602006019203T DE602006019203T DE602006019203D1 DE 602006019203 D1 DE602006019203 D1 DE 602006019203D1 DE 602006019203 T DE602006019203 T DE 602006019203T DE 602006019203 T DE602006019203 T DE 602006019203T DE 602006019203 D1 DE602006019203 D1 DE 602006019203D1
Authority
DE
Germany
Prior art keywords
biological sample
nucleic acid
jump
alkali
sample preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006019203T
Other languages
English (en)
Inventor
Kui Gao
Michael M Becker
Wen Wu
Jeffrey M Linnen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE602006019203D1 publication Critical patent/DE602006019203D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE602006019203T 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali-stoss Active DE602006019203D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US65419905P 2005-02-18 2005-02-18
US66919205P 2005-04-06 2005-04-06
PCT/US2006/005725 WO2006089154A1 (en) 2005-02-18 2006-02-17 Sample preparation method incorporating an alkaline shock

Publications (1)

Publication Number Publication Date
DE602006019203D1 true DE602006019203D1 (de) 2011-02-10

Family

ID=36603476

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006019203T Active DE602006019203D1 (de) 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali-stoss

Country Status (9)

Country Link
US (4) US7510837B2 (de)
EP (2) EP1853706B1 (de)
JP (1) JP4819064B2 (de)
AT (1) ATE493494T1 (de)
AU (1) AU2006214141B2 (de)
CA (1) CA2597319C (de)
DE (1) DE602006019203D1 (de)
DK (1) DK1853706T3 (de)
WO (1) WO2006089154A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE493494T1 (de) 2005-02-18 2011-01-15 Gen Probe Inc Probenherstellungsverfahren mit einem alkali- stoss
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US10503872B2 (en) * 2006-09-29 2019-12-10 Gearbox Llc Computational systems for biomedical data
EP2811037B1 (de) * 2008-04-21 2017-04-19 Gen-Probe Incorporated Verfahren zur Detektion des Chikungunya-Virus
CN102985566B (zh) * 2010-06-30 2015-06-17 简.探针公司 使用分析物和过程控制信号的单次读取确定而鉴定含分析物的样品的方法和设备
EP3589408A1 (de) 2017-03-03 2020-01-08 Gen-Probe Incorporated Verdampfungsbegrenzende einsätze für reagenzbehälter und zugehörige verfahren zur verwendung
CN110505913A (zh) 2017-03-24 2019-11-26 简·探针公司 用于混合容器内容物的系统及相关使用方法
AU2018300000B2 (en) 2017-07-10 2021-11-11 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
CN111902213B (zh) 2018-01-29 2022-12-06 简·探针公司 分析系统和方法
US20210269854A1 (en) * 2018-06-28 2021-09-02 Gen-Probe Incorporated Sample preparation method and system
CA3105684A1 (en) 2018-07-10 2020-01-16 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
US20220396786A1 (en) * 2019-11-14 2022-12-15 Gen-Probe Incorporated Compositions and Methods for Capturing Target Nucleic Acids

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0276302B1 (de) 1986-08-11 1993-04-28 Siska Diagnostics,Inc. Methoden und zusammensetzungen für tests mit nukleinsäuresonden
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
JP2774121B2 (ja) 1987-07-31 1998-07-09 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 標的ポリヌクレオチド配列の選択的増幅
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US4895650A (en) 1988-02-25 1990-01-23 Gen-Probe Incorporated Magnetic separation rack for diagnostic assays
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP3276955B2 (ja) 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ATE257860T1 (de) 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
AU681082B2 (en) 1992-05-06 1997-08-21 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
JP4372837B2 (ja) 1992-08-04 2009-11-25 ジェン−プローブ・インコーポレイテッド 核酸配列増幅
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
AU689789B2 (en) 1993-07-23 1998-04-09 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
DE69535240T2 (de) * 1994-10-28 2007-06-06 Gen-Probe Inc., San Diego Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
ZA973367B (en) * 1996-04-19 1997-11-18 Innogenetics Nv Method for typing and detecting HBV.
US6534273B2 (en) * 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DE69836012T2 (de) 1997-05-02 2007-04-05 Gen-Probe Inc., San Diego Zwei-schritt hybridisierung und einfang von einem polynukleotid
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
JP3856174B2 (ja) * 1997-12-11 2006-12-13 東洋紡績株式会社 植物ｄｎａの抽出精製方法
DE60014399T2 (de) * 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US6682884B2 (en) * 2002-01-03 2004-01-27 Vijay Sharma Method and device for the rapid clinical diagnosis of hepatitis B virus (HBV) infection in biological samples
WO2003106714A1 (en) * 2002-06-14 2003-12-24 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
KR20070097430A (ko) * 2004-11-05 2007-10-04 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
ATE493494T1 (de) 2005-02-18 2011-01-15 Gen Probe Inc Probenherstellungsverfahren mit einem alkali- stoss

Also Published As

Publication number Publication date
US8071301B2 (en) 2011-12-06
CA2597319A1 (en) 2006-08-24
US20060188912A1 (en) 2006-08-24
AU2006214141B2 (en) 2010-07-15
US20120071360A1 (en) 2012-03-22
US8420317B2 (en) 2013-04-16
WO2006089154A1 (en) 2006-08-24
US20110008847A1 (en) 2011-01-13
US20090191596A1 (en) 2009-07-30
AU2006214141A1 (en) 2006-08-24
JP2008529553A (ja) 2008-08-07
US7803581B2 (en) 2010-09-28
DK1853706T3 (da) 2011-03-07
CA2597319C (en) 2014-09-30
JP4819064B2 (ja) 2011-11-16
EP1853706A1 (de) 2007-11-14
EP2243832A3 (de) 2011-01-05
EP2243832A2 (de) 2010-10-27
US7510837B2 (en) 2009-03-31
ATE493494T1 (de) 2011-01-15
EP1853706B1 (de) 2010-12-29

Similar Documents

Publication Publication Date Title
DE602006019203D1 (de) Probenherstellungsverfahren mit einem alkali-stoss
ATE422214T1 (de) Reagenz-kit zur vorbereitung einer testlösung für eine nukleinsäureamplifizierungsreaktion und methode zur ermittlung von nukleinsäuren
BRPI0610092B8 (pt) métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado
DE602007012455D1 (de) Verfahren zur quantifizierung von cholesterin in kleinem dichtem low-density-lipoprotein
ATE546738T1 (de) Verfahren zum schütteln einer lösung
SA521421271B1 (ar) تركيبة وطريقة لثبات الأحماض النووية في عينات بيولوجية
ATE551433T1 (de) Verfahren und produkte zum erfassen von nukleinsäurezielen
EA201101141A1 (ru) Получение закрытой линейной днк
WO2010138182A3 (en) Methods and apparatus for measuring analytes
BR0311175A (pt) Processo e sistema de reagente com complexo de enzima-coenzima não regenerável
Jia et al. Extended GR-5 DNAzyme-based Autonomous isothermal Cascade machine: An efficient and sensitive one-tube colorimetric platform for Pb2+ detection
DE602006010764D1 (de) Modifikatoren der Lichtemission und ihre Verwendungen zur Detektion, Amplifikation und Analyse von Nukleinsäuren
DE602007008953D1 (de) Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung
WO2009060847A1 (ja) 核酸増幅用サンプルの調製方法及び調製キット
MX2012001000A (es) Metodo y kit para la deteccion de un microorganismo.
WO2010002938A3 (en) Method for direct amplification from crude nucleic acid samples
MX2010000031A (es) Deteccion mejorada de microorganismos.
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
Wang et al. Inhibition of double-stranded DNA templated copper nanoparticles as label-free fluorescent sensors for L-histidine detection
Zhang et al. A label-free colorimetric biosensor for sensitive detection of vascular endothelial growth factor-165
RU2014149089A (ru) Способ определения биологической активности дефибротида, основанный на применении эуглобулина
CN103951673B (zh) 一种试剂及其在硫醇检测中的应用
ATE538216T1 (de) Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids
US10889854B2 (en) System and method for immobilization free electrochemiluminescence DNA detection using a luminophore dye for multi-species detection
WO2011025329A3 (en) Detecting methods of dna amplification using new intercalating agent