WO2011025329A3 - Detecting methods of dna amplification using new intercalating agent - Google Patents

Detecting methods of dna amplification using new intercalating agent Download PDF

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Publication number
WO2011025329A3
WO2011025329A3 PCT/KR2010/005840 KR2010005840W WO2011025329A3 WO 2011025329 A3 WO2011025329 A3 WO 2011025329A3 KR 2010005840 W KR2010005840 W KR 2010005840W WO 2011025329 A3 WO2011025329 A3 WO 2011025329A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
dna amplification
intercalating agent
detecting methods
disclosed
Prior art date
Application number
PCT/KR2010/005840
Other languages
French (fr)
Other versions
WO2011025329A2 (en
Inventor
Jun Hee Lee
Hwang Seo Park
Eun Su Han
Jae Don Lee
Han Oh Park
Original Assignee
Bioneer Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioneer Corporation filed Critical Bioneer Corporation
Publication of WO2011025329A2 publication Critical patent/WO2011025329A2/en
Publication of WO2011025329A3 publication Critical patent/WO2011025329A3/en

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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1007—Non-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1011—Condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1014—Carbocyclic compounds bridged by heteroatoms, e.g. N, P, Si or B
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1029—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1029—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom
    • C09K2211/1037—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom with sulfur

Abstract

Disclosed are a fluorescent composition for staining DNA and a method for measuring DNA amplification using the same. More particularly, disclosed are a novel fluorescent compound that emits light by intercalating with DNA and a method for measuring DNA amplification using the same. The novel fluorescent compound is advantageous in that, due to high sensitivity, target-specific amplification products by real-time polymerase chain reaction(PCR) can be accurately quantified without affecting the PCR reaction. In addition, the compound is unharmful to the human body, can be prepared at low cost, and allows monitoring of various DNA and RNA reactions in vitro or in vivo.
PCT/KR2010/005840 2009-08-31 2010-08-30 Detecting methods of dna amplification using new intercalating agent WO2011025329A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090081031A KR101438747B1 (en) 2009-08-31 2009-08-31 Detecting methods of DNA amplication using new intercalating agent
KR10-2009-0081031 2009-08-31

Publications (2)

Publication Number Publication Date
WO2011025329A2 WO2011025329A2 (en) 2011-03-03
WO2011025329A3 true WO2011025329A3 (en) 2011-07-21

Family

ID=43628643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/005840 WO2011025329A2 (en) 2009-08-31 2010-08-30 Detecting methods of dna amplification using new intercalating agent

Country Status (2)

Country Link
KR (1) KR101438747B1 (en)
WO (1) WO2011025329A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103265947B (en) * 2013-06-03 2016-01-20 山东大学 A kind of indolepyridinium salt fluorescent probe for RNA in viable cell and kernel imaging
CN106317018B (en) * 2016-07-21 2018-11-02 湖南大学 A kind of tumor-targeting lipophilic cation-Chlorambucil compound and preparation method and the application in albumin nano drug
CN113527256B (en) * 2020-04-21 2024-02-06 香港科技大学 Aggregation-induced emission fluorescent agent for cancer cell identification and monitoring
CN112239455B (en) * 2020-07-22 2021-08-27 山东大学 RNA fluorescent probe for rapidly distinguishing cancer tissues from normal tissues by using morphological change of nucleolus

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006004267A1 (en) * 2004-03-25 2006-01-12 Bioneer Corporation Detection method of dna amplification using probe labeled with intercalating dye

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006004267A1 (en) * 2004-03-25 2006-01-12 Bioneer Corporation Detection method of dna amplification using probe labeled with intercalating dye

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MACKAY, I. M. ET AL.: "Real-time PCR in virology", NUCLEIC ACIDS RESEARCH, vol. 30, no. 6, 2002, pages 1292 - 1305 *
NEWBY,D.T. ET AL.: "Real-Time PCR Detection of Brucella abortus:a Comparative Study of SYBR Green I, 5'-exonuclease, and Hybridization Probe Assays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 8, 2003, pages 4753 - 4759 *
ZIPPER,H. ET AL.: "Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications", NUCLEIC ACIDS RESEARCH, vol. 32, no. 12, 2004, pages E103 *

Also Published As

Publication number Publication date
WO2011025329A2 (en) 2011-03-03
KR101438747B1 (en) 2014-09-05
KR20110023265A (en) 2011-03-08

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