KR102558606B1 - 결장 유사장기 및 이를 제조 및 사용하는 방법 - Google Patents

결장 유사장기 및 이를 제조 및 사용하는 방법 Download PDF

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KR102558606B1
KR102558606B1 KR1020197018724A KR20197018724A KR102558606B1 KR 102558606 B1 KR102558606 B1 KR 102558606B1 KR 1020197018724 A KR1020197018724 A KR 1020197018724A KR 20197018724 A KR20197018724 A KR 20197018724A KR 102558606 B1 KR102558606 B1 KR 102558606B1
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cells
hco
bmp
signaling pathway
colon
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KR20190088527A (ko
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제임스 엠. 웰스
조지 올란도 무네라
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칠드런즈 호스피탈 메디칼 센터
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KR1020197018724A 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법 Active KR102558606B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020237024207A KR102807995B1 (ko) 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662429948P 2016-12-05 2016-12-05
US62/429,948 2016-12-05
US201762478962P 2017-03-30 2017-03-30
US62/478,962 2017-03-30
PCT/US2017/064600 WO2018106628A1 (en) 2016-12-05 2017-12-05 Colonic organoids and methods of making and using same

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KR1020237024207A Division KR102807995B1 (ko) 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법

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KR20190088527A KR20190088527A (ko) 2019-07-26
KR102558606B1 true KR102558606B1 (ko) 2023-07-26

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KR1020257015203A Pending KR20250068799A (ko) 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법
KR1020237024207A Active KR102807995B1 (ko) 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법

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KR1020237024207A Active KR102807995B1 (ko) 2016-12-05 2017-12-05 결장 유사장기 및 이를 제조 및 사용하는 방법

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US (2) US11767515B2 (https=)
EP (1) EP3548507A4 (https=)
JP (3) JP7068305B2 (https=)
KR (3) KR102558606B1 (https=)
CN (2) CN118909916A (https=)
AU (3) AU2017373767B2 (https=)
CA (1) CA3045145A1 (https=)
IL (1) IL267109A (https=)
NZ (1) NZ753873A (https=)
SG (1) SG10202105768WA (https=)
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Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
AU2015331848B2 (en) 2014-10-17 2022-03-03 Children's Hospital Medical Center, D/B/A Cincinnati Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
EP4553082A3 (en) 2016-11-04 2025-08-20 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
JP7248586B2 (ja) 2017-04-14 2023-03-29 チルドレンズ ホスピタル メディカル センター 複数ドナー幹細胞組成物およびそれを作製する方法
EP3625331B1 (en) * 2017-05-19 2024-07-10 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
EP3787613B1 (en) 2018-04-30 2026-03-11 Cedars-Sinai Medical Center Pkc activation for use in treating early onset sporadic parkinson's disease
EP4678727A3 (en) 2018-07-26 2026-03-11 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CA3112026A1 (en) * 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same
US20220220448A1 (en) * 2019-05-15 2022-07-14 The Cleveland Clinic Foundation Induced human colitic organoids
AU2020283048A1 (en) 2019-05-31 2021-12-23 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US20220411747A1 (en) * 2019-10-31 2022-12-29 University Health Network Medium and methods for culturing organoids
CN111849860B (zh) * 2020-06-01 2021-12-21 浙江大学 一种利用铁元素调控肠道干细胞分化的方法及应用
WO2022025269A1 (ja) * 2020-07-30 2022-02-03 国立研究開発法人理化学研究所 膀胱オルガノイド及びその製造方法
CN113009155B (zh) * 2021-03-05 2022-03-25 温州医科大学慈溪生物医药研究院 Fgf20在制备诊断溃疡性结肠炎试剂的应用
IL281561A (en) 2021-03-16 2022-10-01 Yeda Res & Dev Methods and devices for ex-utero mouse embryonic development
CN113388572B (zh) * 2021-08-17 2021-11-19 天九再生医学(天津)科技有限公司 高效人多能干细胞体外诱导分化为多谱系小肠类器官方法
EP4388083A4 (en) * 2021-08-19 2025-07-09 Childrens Hospital Med Ct VASCULARIZED ORGANOIDS
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US12435335B2 (en) 2022-06-06 2025-10-07 Cornell University Promoting nutrient absorption through the colon
KR102843172B1 (ko) * 2023-01-04 2025-08-05 연세대학교 산학협력단 불일치 복구 유전자 기능 결핍 환자에서 환자 유래 오가노이드를 이용한 개인별 dna 손상 반응 평가 및 암 위험도 예측
WO2024192567A1 (zh) * 2023-03-17 2024-09-26 北京大学 建立新型人肠道及结直肠肿瘤类器官培养体系
EP4731750A2 (en) 2023-06-23 2026-04-29 Children's Hospital Medical Center Methods of matrix-free suspension culture
WO2025035155A1 (en) * 2023-08-10 2025-02-13 The Regents Of The University Of California Foxo inhibition to augment gastrointestinal function and paneth cell function
WO2025072803A1 (en) 2023-09-29 2025-04-03 Children's Hospital Medical Center Ntrk2 signaling-mediated alveolar capillary injury and repair
CN118501457B (zh) * 2024-04-29 2025-06-17 南通大学附属医院 PPP6R1基因及其RNAi慢病毒干扰系统和应用
CN118240750B (zh) * 2024-05-28 2024-08-13 淇嘉科技(苏州)有限公司 具有免疫细胞的结肠类器官构建方法
CN118374432B (zh) * 2024-06-26 2024-10-01 淇嘉科技(苏州)有限公司 匀质化肠道类器官制备方法
CN118374446B (zh) * 2024-06-26 2024-10-01 淇嘉科技(苏州)有限公司 巨噬细胞包含型结肠类器官批量制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012168930A2 (en) * 2011-06-10 2012-12-13 Koninklijke Nederlandse Akademie Van Wetenschappen (Knaw) Culture media for stem cells
US20130137130A1 (en) * 2010-05-06 2013-05-30 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation

Family Cites Families (311)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9031591A (en) 1990-10-29 1992-05-26 Regents Of The University Of Minnesota A bioartificial liver
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5912227A (en) 1995-01-27 1999-06-15 North Carolina State University Method of enhancing nutrient uptake
WO1998021312A1 (en) 1996-11-08 1998-05-22 Rpms Technology Limited Human hepatocytes in three-dimensional support systems
CA2279996A1 (en) 1997-01-16 1998-07-16 Yissum Research Development Company Of The Hebrew University In Jerusale M A device and method for performing a biological modification of a fluid
US7291626B1 (en) 1998-04-09 2007-11-06 John Hopkins University School Of Medicine Inhibitors of hedgehog signaling pathways, compositions and uses related thereto
US20010041964A1 (en) 1998-09-14 2001-11-15 George M. Grass Pharmacokinetic-based drug design tool and method
US7759113B2 (en) 1999-04-30 2010-07-20 The General Hospital Corporation Fabrication of tissue lamina using microfabricated two-dimensional molds
US6607501B2 (en) 2001-05-14 2003-08-19 Reynolds G. Gorsuch Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
AU2002367580A1 (en) 2001-05-16 2003-09-22 Tracy C. Grikscheit Tissue-engineered organs
IL162131A0 (en) 2001-12-07 2005-11-20 Geron Corp Islet cells from human embryonic stem cells
US20050170506A1 (en) 2002-01-16 2005-08-04 Primegen Biotech Llc Therapeutic reprogramming, hybrid stem cells and maturation
US20030187515A1 (en) 2002-03-26 2003-10-02 Hariri Robert J. Collagen biofabric and methods of preparing and using the collagen biofabric
US7160719B2 (en) 2002-06-07 2007-01-09 Mayo Foundation For Medical Education And Research Bioartificial liver system
WO2004020614A1 (ja) 2002-08-28 2004-03-11 Asahi Medical Co., Ltd. 異形断面中空糸膜型細胞含有デバイス
TW571101B (en) 2003-01-21 2004-01-11 Ind Tech Res Inst Fluid analysis apparatus
WO2004071573A2 (en) 2003-02-07 2004-08-26 The Johns Hopkins University Hypoxia induced mitogenic factor
EP1595867A4 (en) 2003-02-10 2008-05-21 Banyu Pharma Co Ltd PIPERIDINE DERIVATIVES AS AN ACTIVE ANTAGONIST AT THE RECIPE OF MELANIN CONCENTRATING HORMONE
DE10362002B4 (de) 2003-06-23 2006-10-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Adulte pluripotente Stammzellen
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CN103898047B (zh) 2003-12-23 2020-03-03 维亚希特公司 定形内胚层
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US8241905B2 (en) 2004-02-24 2012-08-14 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
DE102004017476B4 (de) 2004-04-08 2009-03-26 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Herstellung einer Epithelzellen enthaltenden Zellzusammensetzung
CN103103158B (zh) 2004-04-27 2016-08-03 韦尔赛特公司 细胞培养基
JP4650608B2 (ja) 2004-05-18 2011-03-16 信越化学工業株式会社 フォトマスクブランク及びフォトマスクの製造方法
EP1747264B1 (de) 2004-05-21 2012-09-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Multizelluläre gewebe- und organkultursysteme
US8410246B2 (en) 2004-06-17 2013-04-02 Thrasos, Inc. TDF-related compounds and analogs thereof
AU2005272078B2 (en) 2004-07-09 2011-04-14 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
MX2007000510A (es) 2004-07-12 2007-03-29 Emisphere Tech Inc Composiciones para suministrar peptido yy y agonistas de pyy.
WO2006091231A2 (en) 2004-07-21 2006-08-31 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
US20060236415A1 (en) 2005-03-09 2006-10-19 Silversides David W Neural crest cells specific promoters; isolated neural crest cells; and methods of isolating and of using same
US7604929B2 (en) 2005-04-21 2009-10-20 In Vitro Technologies, Inc. Cellular compositions and methods for their preparation
US8435787B2 (en) 2005-05-10 2013-05-07 Rutgers, The State University Of New Jersey Alginate polyelectrolyte encapsulation of embryonic stem cells
WO2006126219A1 (en) 2005-05-26 2006-11-30 Fresenius Medical Care Deutschland G.M.B.H. Liver progenitor cells
GB0517382D0 (en) 2005-08-26 2005-10-05 Plasticell Ltd Cell culture
WO2007027905A2 (en) 2005-08-31 2007-03-08 Science And Technology Corporation @ Unm Human renal stem cells
WO2007051038A2 (en) 2005-10-27 2007-05-03 Cythera, Inc. Pdx1-expressing dorsal and ventral foregut endoderm
US7927869B2 (en) 2005-11-29 2011-04-19 Spencer Z Rosero System and method for supporting a biological chip device
US20070239083A1 (en) 2006-01-18 2007-10-11 Axel Voss Shock wave generators
US20070238169A1 (en) 2006-04-11 2007-10-11 The Board Of Trustees Of The Leland Stanford Junior University Cell sorter and culture system
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
WO2007136707A2 (en) 2006-05-17 2007-11-29 University Of Utah Research Foundation Methods and compositions related to eosinophil regulation
PT2040713E (pt) 2006-06-27 2014-10-13 Intercept Pharmaceuticals Inc Para a prevenção ou o tratamento de doenças ou estados clínicos mediados por fxr
EP2046266A4 (en) 2006-07-21 2009-11-04 Massachusetts Inst Technology ENDMODICIFIED POLY (BETA AMINO ESTER) AND ITS USE
US8497240B2 (en) 2006-08-17 2013-07-30 Amylin Pharmaceuticals, Llc DPP-IV resistant GIP hybrid polypeptides with selectable properties
WO2008075339A2 (en) 2006-12-18 2008-06-26 Ben Gurion University Of The Negev Scaffolding for tissue regeneration or repair
FR2917425B1 (fr) 2007-06-18 2010-11-19 Univ Nancy 1 Henri Poincare Procede de proliferation de cellules sur des multicouches de polyelectrolytes et son application, notamment a la preparation de biomateriaux cellularises
GB2467645B (en) 2007-07-06 2011-12-21 Univ London A method of proliferating human hepatocyte cells
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
WO2009033011A1 (en) * 2007-09-07 2009-03-12 Children's Hospital Medical Center Use of secretor, lewis and sialyl antigen levels as predictors for disease
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
EP2235161A1 (en) 2007-12-11 2010-10-06 Research Development Foundation Small molecules for neuronal differentiation of embryonic stem cells
AU2009203893B2 (en) 2008-01-08 2014-10-02 The University Of Queensland Method of producing a population of cells
US20110218512A1 (en) 2008-06-03 2011-09-08 Aethlon Medical, Inc. Enhanced antiviral therapy methods and devices
WO2009146911A2 (en) 2008-06-04 2009-12-10 Uwe Marx Organ-on-a-chip-device
JP5624981B2 (ja) 2008-06-24 2014-11-12 ザ・キュレーターズ・オブ・ザ・ユニバーシティ・オブ・ミズーリＴｈｅ Ｃｕｒａｔｏｒｓ Ｏｆｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｍｉｓｓｏｕｒｉ 自己集合性多細胞体、および前記多細胞体を用いて３次元の生物構造体を作製する方法
ITRM20080342A1 (it) 2008-06-26 2009-12-27 Univ Degli Studi Udine Cellule di polpa dentale midollo-simili, metodi per isolamento ed uso.
US20130115673A1 (en) 2008-07-16 2013-05-09 Biotime, Inc. Methods of Screening Embryonic Progenitor Cell Lines
US20110305672A1 (en) 2008-07-25 2011-12-15 University Of Georgia Research Foundation, Inc. COMPOSITIONS FOR MESODERM DERIVED ISL1+ MULTIPOTENT CELLS (IMPs), EPICARDIAL PROGENITOR CELLS (EPCs) AND MULTIPOTENT CD56C CELLS (C56Cs) AND METHODS OF PRODUCING AND USING SAME
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US20110294735A1 (en) 2008-11-05 2011-12-01 Merck Sharp & Dohme Corp. Mechanism of neuromedin u action and uses thereof
JP5351601B2 (ja) 2008-12-26 2013-11-27 矢崎総業株式会社 絶縁キャップの製造方法及び絶縁キャップの製造装置
EP2393917B1 (en) 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
WO2010094694A1 (en) 2009-02-23 2010-08-26 F. Hoffmann-La Roche Ag Assays to predict cardiotoxicity
CN102421467B (zh) 2009-03-13 2015-04-22 梅约医学教育与研究基金会 生物人工肝
JP2012520866A (ja) 2009-03-17 2012-09-10 アプタリス・ファーマ・カナダ・インコーポレイテッド 高用量のウルソデオキシコール酸で非アルコール性脂肪性肝炎を治療する方法
WO2010127399A1 (en) 2009-05-06 2010-11-11 Walter And Eliza Hall Institute Of Medical Research Gene expression profiles and uses thereof
WO2014066811A1 (en) 2012-10-25 2014-05-01 The Johns Hopkins University Bioreducible poly (b-amino ester)s for sirna delivery
CA2762584A1 (en) 2009-05-20 2010-11-25 Cardio3 Biosciences S.A. Pharmaceutical composition for the treatment of heart diseases
US20120135519A1 (en) 2009-05-29 2012-05-31 Cellartis Ab INDUCED DERIVATION OF SPECIFIC ENDODERM FROM hPS CELL-DERIVED DEFINITIVE ENDODERM
WO2010143747A1 (ja) 2009-06-10 2010-12-16 公立大学法人奈良県立医科大学 人工腸管の作製法
WO2010149597A2 (en) 2009-06-18 2010-12-29 Cellartis Ab 3D CULTURING SYSTEMS FOR GROWTH AND DIFFERENTIATION OF HUMAN PLURIPOTENT STEM (hPS) CELLS
CN105796601A (zh) 2009-07-16 2016-07-27 生物时代股份有限公司 用于体外和体内软骨发生的方法和组合物
US8926561B2 (en) 2009-07-30 2015-01-06 Tandem Diabetes Care, Inc. Infusion pump system with disposable cartridge having pressure venting and pressure feedback
US8501476B2 (en) 2009-10-07 2013-08-06 Brown University Assays and methods for fusing cell aggregates to form proto-tissues
CN101696396A (zh) 2009-10-27 2010-04-21 中国人民解放军军事医学科学院卫生学环境医学研究所 乙型肝炎病毒体外感染模型的构建方法及应用
CN102740888B (zh) 2009-11-24 2016-10-12 奥尔德生物制药公司 Il-6抗体及其用途
WO2011064309A1 (en) 2009-11-25 2011-06-03 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for hepatic differentiation of definitive endoderm cells
CN102712902B (zh) 2009-12-23 2019-01-08 詹森生物科技公司 人胚胎干细胞的分化
US20110231942A1 (en) 2010-03-17 2011-09-22 Xi He Tiki1 and tiki2, wnt inhibitors
US9394522B2 (en) 2010-03-22 2016-07-19 Takara Bio Europe Ab Directed differentiation and maturation of pluripotent cells into hepatocyte like cells by modulation of Wnt-signalling pathway
EP2380920A1 (en) 2010-04-22 2011-10-26 QGel SA Hydrogel precursor formulation and production process thereof
EP2563908B1 (en) 2010-04-25 2019-01-09 Icahn School of Medicine at Mount Sinai Generation of anterior foregut endoderm from pluripotent cells
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
EP2609191B1 (en) 2010-08-24 2017-11-22 Regents Of The University Of Minnesota Non-static suspension culture of cell aggregates
CN103154237B (zh) 2010-08-31 2016-03-16 詹森生物科技公司 多能干细胞的分化
WO2012068170A2 (en) 2010-11-15 2012-05-24 Jau-Nan Lee Generation of neural stem cells from human trophoblast stem cells
EP2658965B1 (en) 2010-12-31 2016-03-02 Universität für Bodenkultur Wien Method of generating induced pluripotent stem cells and differentiated cells
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9200258B2 (en) 2011-01-27 2015-12-01 University Of Maryland, Baltimore Multicellular organotypic model of human intestinal mucosa
EP2484750A1 (en) 2011-02-07 2012-08-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Monitoring system for cell culture
DK2681306T3 (en) 2011-02-28 2019-04-23 Harvard College CELL CULTURE SYSTEM
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
US20140158233A1 (en) 2011-05-09 2014-06-12 President And Fellows Of Harvard College Aerosol delivery to a microfluidic device
US10260039B2 (en) 2011-05-11 2019-04-16 Massachusetts Institute Of Technology Microgels and microtissues for use in tissue engineering
AU2012262139B2 (en) 2011-06-02 2017-02-23 Children's Medical Center Corporation Methods and uses for ex vivo tissue culture systems
CA2840192A1 (en) 2011-06-23 2012-12-27 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
EP2755599B1 (en) 2011-09-12 2020-04-01 Organovo, Inc. Platform for engineered implantable tissues and organs and methods of making the same
JP2013066414A (ja) 2011-09-22 2013-04-18 National Institute Of Advanced Industrial Science & Technology 胃前駆細胞の表面マーカー
WO2013063588A1 (en) 2011-10-28 2013-05-02 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of primary tissue organoids
ES2689265T3 (es) 2011-11-04 2018-11-12 Inregen Cribado de fármacos y ensayos de potencia
EP2788476B1 (en) 2011-12-08 2019-05-22 Fred Hutchinson Cancer Research Center Compositions and methods for enhanced generation of hematopoietic stem/progenitor cells
US9725687B2 (en) 2011-12-09 2017-08-08 President And Fellows Of Harvard College Integrated human organ-on-chip microphysiological systems
US10087422B2 (en) 2011-12-09 2018-10-02 President And Fellows Of Harvard College Organ chips and uses thereof
US10006904B2 (en) 2011-12-19 2018-06-26 Umc Utrecht Holding B.V. Rapid quantitative assay to measure CFTR function in a primary intestinal culture model
CN113699105A (zh) 2011-12-23 2021-11-26 人类起源公司 包含脱细胞并再群体化的胎盘血管支架的类器官
US9828583B2 (en) 2012-01-13 2017-11-28 The General Hospital Corporation Isolated human lung progenitor cells and uses thereof
US9675646B2 (en) 2012-01-31 2017-06-13 Wake Forest University Health Sciences Tubular bioengineered smooth muscle structures
AU2013222188A1 (en) 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
EP2634251A1 (en) 2012-02-29 2013-09-04 Technische Universität Berlin 3D in vitro bi-phasic cartilage-bone construct
KR20150013471A (ko) 2012-04-09 2015-02-05 토마스 제이. 굿윈 교류 이온 자기 공명(ａｉｍｒ) 멀티-챔버-배양 장치 및 사용 방법
KR20160027219A (ko) 2012-05-23 2016-03-09 에프. 호프만-라 로슈 아게 내배엽 및 간세포를 수득하고 사용하는 조성물 및 방법
US20140099709A1 (en) 2012-06-19 2014-04-10 Organovo, Inc. Engineered three-dimensional connective tissue constructs and methods of making the same
NZ734451A (en) 2012-06-19 2018-12-21 Intercept Pharmaceuticals Inc Preparation, uses and solid forms of obeticholic acid
DE102012105540A1 (de) 2012-06-26 2014-04-24 Karlsruher Institut für Technologie Gefäßmodell, Verfahren zu seiner Herstellung und seine Verwendung
WO2014013334A2 (en) 2012-07-20 2014-01-23 Agency For Science, Technology And Research In vitro assay for predicting renal proximal tubular cell toxicity
US9988606B2 (en) 2012-07-24 2018-06-05 The Trustees Of Columbia University In The City Of New York Generation of airway and lung progenitors and epithelial cells and three-dimensional anterior foregut spheres
GB201216796D0 (en) 2012-09-20 2012-11-07 Cambridge Entpr Ltd In vitro pancreatic differentiation
DK2712918T3 (en) 2012-09-28 2015-02-16 Tissuse Gmbh Multi-organ-chip with improved lifetime and homeostasis
EP2716298A1 (en) 2012-10-03 2014-04-09 Institut Pasteur A nod2-dependant pathway of cytoprotection of stem cells
US20150329821A1 (en) * 2012-10-19 2015-11-19 Agency For Science, Technology And Research Methods of differentiating stem cells into one or more cell lineages
EP2735326B1 (en) 2012-11-26 2017-03-08 Gambro Lundia AB Liver support system
WO2014082096A1 (en) 2012-11-26 2014-05-30 The Trustees Of Columbia University In The City Of New York Method for culture of human and mouse prostate organoids and uses thereof
EP3031921B1 (en) 2012-12-12 2025-03-12 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
EP2743345A1 (en) 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Three dimensional heterogeneously differentiated tissue culture
US20150359849A1 (en) 2013-01-31 2015-12-17 President And Fellows Of Harvard College Methods of increasing neuronal connectivity and/or treating a neurodegenerative condition
WO2014127170A1 (en) 2013-02-13 2014-08-21 Wake Forest University Health Sciences Bioengineered liver constructs and methods relating thereto
GB201304245D0 (en) 2013-03-08 2013-04-24 Inst Quimic De Sarria Chemical compounds
WO2014165056A1 (en) 2013-03-12 2014-10-09 Board Of Regents, The University Of Texas System High throughput mechanical strain generating system for cell cultures and applications thereof
HUE043785T2 (hu) 2013-03-13 2019-09-30 Wisconsin Alumni Res Found Módszerek és anyagok humán pluripotens õssejtek hematoendothelialis differenciálására meghatározott körülmények között
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
WO2014152906A2 (en) 2013-03-14 2014-09-25 Research Institute At Nationwide Children's Hospital, Inc. Tissue engineered intestine
CA2899507A1 (en) 2013-03-14 2014-09-25 The Regents Of The University Of California In vitro production of medial ganglionic eminence precursor cells
PL2970890T3 (pl) 2013-03-14 2020-11-16 The Brigham And Women's Hospital, Inc. Kompozycje i sposoby ekspansji i hodowli nabłonkowych komórek macierzystych
US9442105B2 (en) 2013-03-15 2016-09-13 Organovo, Inc. Engineered liver tissues, arrays thereof, and methods of making the same
US20160237400A1 (en) 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
WO2014153294A1 (en) 2013-03-17 2014-09-25 The Regents Of The University Of California Method to expand and transduce cultured human small and large intestinal stem cells
US9677085B2 (en) 2013-03-18 2017-06-13 Massachusetts Institute Of Technology Engineering a heterogeneous tissue from pluripotent stem cells
EP2796873A1 (en) 2013-04-25 2014-10-29 QGel SA Method for a cell-based drug screening assay and the use thereof
US20160245653A1 (en) 2013-04-30 2016-08-25 Sangtae Park Cylindrical resonator gyroscope
JP6490669B2 (ja) 2013-05-08 2019-03-27 インリージェン 単離腎細胞を含有するオルガノイド及びその使用
US10545133B2 (en) 2013-05-13 2020-01-28 The Johns Hopkins University Molecular signatures of invasive cancer subpopulations
WO2014197622A2 (en) 2013-06-04 2014-12-11 The Regents Of The University Of California Liver-mimetic device and method for simulation of hepatic function using such device
JP6253265B2 (ja) 2013-06-05 2017-12-27 学校法人関西医科大学 食道上皮幹細胞の単離方法
AU2014279568B2 (en) 2013-06-10 2020-03-12 Corning Incorporated Tissue structure and manufacturing method therefor
RU2016100232A (ru) 2013-06-14 2017-07-17 Дзе Юниверсити Оф Квинсленд Почечные клетки-предшественники
BR112016000706B1 (pt) 2013-07-23 2022-08-16 Public University Corporation Yokohama City University Método de integração de um tecido biológico com um sistema vascular in vitro, tecido biológico, método de avaliação de um fármaco, e composição
AU2014305843B2 (en) 2013-08-09 2019-08-29 Ardelyx, Inc. Compounds and methods for inhibiting phosphate transport
ES2764408T3 (es) 2013-08-28 2020-06-03 Promethera Biosciences S A / N V Procedimiento para la producción de células progenitoras hepáticas adultas
GB201317869D0 (en) 2013-10-09 2013-11-20 Cambridge Entpr Ltd In vitro production of foregut stem cells
EP3060650A4 (en) 2013-10-25 2017-04-19 Agency For Science, Technology And Research Culturing pluripotent stem cells
EP3071686B1 (en) 2013-11-22 2020-07-22 Cellectis SA Method for generating batches of allogeneic t-cells with averaged potency
ES2732730T3 (es) 2013-11-22 2019-11-25 Riken Método para producir telencéfalo o tejido progenitor del mismo
EP2876441B1 (en) 2013-11-26 2017-10-25 Bergen Teknologioverforing AS Quantitative analysis of contact-depending cell-to-cell transfer and disease transmission
EP3574985A1 (en) 2013-12-20 2019-12-04 President And Fellows Of Harvard College Organomimetic devices and methods of use and manufacturing thereof
KR102611973B1 (ko) 2014-01-14 2023-12-08 예일 유니버시티 기도 세포의 제조를 위한 조성물 및 방법
US11648335B2 (en) 2014-01-31 2023-05-16 Wake Forest University Health Sciences Organ/tissue decellularization, framework maintenance and recellularization
EA201691607A1 (ru) 2014-02-11 2017-01-30 Антродженезис Корпорейшн Микроорганоиды и способы их получения и применения
US10369254B2 (en) 2014-02-26 2019-08-06 The Regents Of The University Of California Method and apparatus for in vitro kidney organogenesis
CN106062181A (zh) 2014-02-27 2016-10-26 公立大学法人横滨市立大学 自发组织用细胞集合体的制作方法
DE102014003465A1 (de) 2014-03-11 2015-09-17 NeuroProof GmbH Gewinnung von Gehirnregion-spezifischen neuronalen Kulturen aus dreidimensionalen Gewebekulturen von Stammzellen
US11066649B2 (en) 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
EP3119401A4 (en) 2014-03-21 2017-12-13 Tobira Therapeutics, Inc. Cenicriviroc for the treatment of fibrosis
EP3808838B1 (en) 2014-04-04 2024-04-10 Organovo, Inc. Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
US10222370B2 (en) 2014-04-07 2019-03-05 Rush University Medical Center Screening assay for choice of prebiotic to prevent/treat gastrointestinal and systemic diseases
WO2015156929A1 (en) 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
KR102374499B1 (ko) 2014-04-11 2022-03-14 사이머베이 쎄라퓨틱스, 인코퍼레이티드 Nafld 및 nash 의 치료
MX388202B (es) 2014-04-27 2025-03-19 Univ New York State Res Found Métodos para generar productos de esmalte in vitro.
CA2949171A1 (en) 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
CN106536707B (zh) 2014-05-29 2018-12-25 西奈山伊坎医学院 在生物反应器系统中制造心脏类器官的方法和装置
US10426757B2 (en) 2014-05-29 2019-10-01 Whitehead Institute For Biomedical Research Compositions and methods for promoting intestinal stem cell and/or non-stem progenitor cell function
EP3786285A1 (en) 2014-06-05 2021-03-03 Cedars-Sinai Medical Center A novel and efficient method for reprogramming immortalized lymphoblastoid cell lines to induced pluripotent stem cells
EP3152296B1 (en) 2014-06-06 2021-08-04 Vrije Universiteit Brussel Human hepatic 3d co-culture model and uses thereof
EP3158056B1 (en) 2014-06-20 2021-10-06 Rutgers, the State University of New Jersey Single cell-derived organoids
US10487314B2 (en) 2014-06-26 2019-11-26 The Trustees Of Columbia University In The City Of New York Inhibition of serotonin expression in gut enteroendocrine cells results in conversion to insulin-positive cells
KR20170063519A (ko) 2014-07-17 2017-06-08 셀매틱스, 인크. 불임 및 관련 병리상태를 평가하기 위한 방법 및 시스템
IL282156B (en) 2014-07-29 2022-07-01 Shenzhen Hightide Biopharmaceutical Ltd Berberine salts, ursodeoxycholic salts and combinations, methods of preparation and application thereof
US12247224B2 (en) 2014-07-30 2025-03-11 Senthil Muthuswamy Organoids for drug screening and personalized medicine
SG11201701398SA (en) 2014-08-22 2017-03-30 Cambridge Entpr Ltd Resetting pluripotent stem cells
KR102101060B1 (ko) 2014-08-28 2020-04-16 프로메테라 바이오사이언시즈 에스.에이./엔.브이. 성체 간 전구 세포의 제조 방법
US20160060707A1 (en) 2014-08-29 2016-03-03 Immunomedics, Inc. Identification of Cancer Genes by In-Vivo Fusion of Human Cancer Cells and Animal Cells
EP3777863A1 (en) 2014-09-12 2021-02-17 Tobira Therapeutics, Inc. Cenicriviroc combination therapy for the treatment of fibrosis
KR20170064547A (ko) 2014-10-06 2017-06-09 오가노보, 인크. 조작된 신장 조직, 이의 어레이, 및 이를 제조하는 방법
US20170304369A1 (en) 2014-10-08 2017-10-26 Agency For Science, Technology And Research Methods of differentiating stem cells into liver cell lineages
EP3207122B1 (en) 2014-10-14 2019-05-08 FUJIFILM Cellular Dynamics, Inc. Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures
AU2015331848B2 (en) 2014-10-17 2022-03-03 Children's Hospital Medical Center, D/B/A Cincinnati Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
US20160121023A1 (en) 2014-10-30 2016-05-05 Massachusetts Institute Of Technology Materials and Methods for Rescue of Ischemic Tissue and Regeneration of Tissue Integrity During Resection, Engraftment and Transplantation
WO2016073989A2 (en) 2014-11-07 2016-05-12 The Trustees Of Columbia University In The City Of New York Osteochondroreticular stem cells for bone and cartilage regeneration
WO2016085765A1 (en) 2014-11-25 2016-06-02 President And Fellows Of Harvard College Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
US20170260501A1 (en) 2014-11-25 2017-09-14 International Stem Cell Corporation Derivation of neural crest stem cells and uses thereof
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
AU2014277667B2 (en) 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
JP7089240B2 (ja) 2014-12-22 2022-06-22 エコール・ポリテクニーク・フェデラル・ドゥ・ローザンヌ （ウ・ペ・エフ・エル） 高い収集能力で哺乳動物細胞を操作（マニピュレーション）する装置
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
EA201791258A1 (ru) 2015-01-09 2017-12-29 Джилид Аполло, Ллс Комбинированная терапия с применением ингибитора ацетил-коа-карбоксилазы (acc) для лечения неалкогольной жировой болезни печени
JP6281850B2 (ja) 2015-01-28 2018-02-21 公立大学法人横浜市立大学 骨髄細胞凝集体の作製方法
US20160256672A1 (en) 2015-02-10 2016-09-08 Cedars-Sinai Medical Center Induced pluripotent stem cell-derived hepatocyte based bioartificial liver device
CN105985395A (zh) 2015-02-13 2016-10-05 江苏奥赛康药业股份有限公司 一种奥贝胆酸化合物及含有该化合物的药物组合物
WO2016140716A1 (en) 2015-03-02 2016-09-09 The Trustees Of Columbia University In The City Of New York Injectable microtissue systems, devices, and methods
CN107427537A (zh) 2015-03-03 2017-12-01 哈佛学院院长及董事 产生功能性人体组织的方法
US20160257937A1 (en) 2015-03-06 2016-09-08 University Of North Carolina At Chapel Hill HUMAN FIBROLAMELLAR HEPATOCELLULAR CARCINOMAS (hFL-HCCS)
JP6800875B2 (ja) 2015-03-06 2020-12-16 マイクロマス ユーケー リミテッド 急速蒸発イオン化質量分析（「ｒｅｉｍｓ」）装置に連結されたイオンアナライザのための流入器具
EA201791982A1 (ru) 2015-03-09 2020-02-17 Интекрин Терапьютикс, Инк. Способы лечения неалкогольной жировой болезни печени и/или липодистрофии
US10023922B2 (en) 2015-03-23 2018-07-17 Whitehead Institute For Biomedical Research Reporter of genomic methylation and uses thereof
CR20170456A (es) 2015-04-07 2018-06-13 Intercept Pharmaceuticals Inc Composiciones farmacéuticas para terapias combinadas
US10087417B2 (en) 2015-04-22 2018-10-02 William J. Freed Three-dimensional model of human cortex
US10557124B2 (en) 2015-04-22 2020-02-11 The Regents Of The University Of Michigan Compositions and methods for obtaining stem cell derived lung tissue, and related uses thereof
CN104877964A (zh) 2015-04-24 2015-09-02 赵振民 一种唾液腺类器官和类腺泡的体外构建方法
PE20180690A1 (es) 2015-04-27 2018-04-23 Intercept Pharmaceuticals Inc Composiciones de acido obeticolico y metodos de uso
WO2016174604A1 (en) 2015-04-30 2016-11-03 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Means and methods for generation of breast stem cells
US11299714B2 (en) 2015-05-11 2022-04-12 The Trustees Of Columbia University In The City Of New York Engineered adult-like human heart tissue
EP3303566B1 (en) 2015-06-03 2020-10-14 Takara Bio Europe AB Maturation of mammalian hepatocytes
GB201510950D0 (en) 2015-06-22 2015-08-05 Cambridge Entpr Ltd In vitro Production of Cholangiocytes
WO2016210313A1 (en) 2015-06-24 2016-12-29 Whitehead Institute For Biomedical Research Culture medium for generating microglia from pluripotent stem cells and related methods
US20180171302A1 (en) 2015-06-26 2018-06-21 Domenico ACCILI Genetically Modified IPS Cells That Carry a Marker to Report Expression of Neurogenin3, TPH2, FOXO1 and/or Insulin Genes
WO2017009263A1 (en) 2015-07-10 2017-01-19 Etablissement Francais Du Sang Method for obtaining human brown/beige adipocytes
US10449221B2 (en) 2015-07-29 2019-10-22 Trustees Of Boston University Differentiation of stem cells into thyroid tissue
WO2017036533A1 (en) 2015-09-03 2017-03-09 Ecole Polytechnique Federale De Lausanne (Epfl) Three-dimensional hydrogels for culturing adult epithelial stem cells and organoids
AU2016318114B2 (en) 2015-09-03 2023-04-20 The Brigham And Women's Hospital, Inc. Three-dimensional differentiation of epiblast spheroids to kidney organoids models stage-specific epithelial physiology, morphogenesis, and disease
EP3350313B1 (en) 2015-09-15 2025-11-26 Agency For Science, Technology And Research (A*star) Derivation of liver organoids from human pluripotent stem cells
JP2018532720A (ja) 2015-09-16 2018-11-08 トビラ セラピューティクス， インコーポレイテッド 線維症の治療のためのセニクリビロック併用療法
US20180258404A1 (en) 2015-09-17 2018-09-13 The Brigham And Women's Hospital, Inc. Methods of generating nephrons from human pluripotent stem cells
US20180273904A1 (en) 2015-10-02 2018-09-27 Wake Forest University Health Sciences Spontaneously beating cardiac organoid constructs and integrated body-on-chip apparatus containing the same
LU92845B1 (en) 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids
WO2017070007A2 (en) 2015-10-15 2017-04-27 Wake Forest University Health Sciences Methods of producing in vitro liver constructs and uses thereof
US10993433B2 (en) 2015-10-15 2021-05-04 Wake Forest University Health Sciences Method of producing in vitro testicular constructs and uses thereof
EP3362554B1 (en) 2015-10-16 2025-09-03 Wake Forest University Health Sciences Multi-layer airway organoids and methods of making and using the same
WO2017066659A1 (en) 2015-10-16 2017-04-20 The Trustees Of Columbia University In The City Of New York Jag1 expression predicts therapeutic response in nash
KR102410389B1 (ko) 2015-10-19 2022-06-16 에뮬레이트, 인크. 혈액 뇌 장벽의 미세유체 모델
US12209253B2 (en) 2016-08-29 2025-01-28 EMULATE, Inc. Development of spinal cord on a microfluidic chip
US10947502B2 (en) 2015-10-20 2021-03-16 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
SG11201803061UA (en) 2015-10-21 2018-05-30 Univ Indiana Res & Tech Corp Methods of generating human inner ear sensory epithelia and sensory neurons
SG11201803043VA (en) 2015-10-21 2018-05-30 Univ Indiana Res & Tech Corp Derivation of human skin organoids from pluripotent stem cells
SG11201803419PA (en) 2015-10-30 2018-05-30 The Regents Of The Universtiy Of California Methods of generating t-cells from stem cells and immunotherapeutic methods using the t-cells
US11866409B2 (en) 2015-11-02 2024-01-09 Carmel-Haifa University Economic Corporation Ltd. Apoptosis related protein in the tgf-beta signaling pathway (ARTS) mimetic compounds, compositions, methods and uses thereof in induction of differentiation and/or apoptosis of premalignant and malignant cells, thereby restoring their normal-like phenotype
WO2017079632A1 (en) 2015-11-04 2017-05-11 Cedars-Sinai Medical Center Patient-derived ctc-xenograft models
HK1257929A1 (zh) 2015-11-06 2019-11-01 Gemphire Therapeutics Inc. 治疗混合性血脂异常
KR102932021B1 (ko) 2015-11-12 2026-02-27 바이오스테이지, 인크. 문합 또는 다른 생리학적 위치에서 위장 조직을 생성하기 위한 시스템 및 방법
WO2017083705A1 (en) 2015-11-13 2017-05-18 The Johns Hopkins University Cell culture system and method of use thereof
EP3383996B1 (en) 2015-12-04 2024-10-16 President and Fellows of Harvard College Devices for simulating a function of a liver tissue and methods of use and manufacturing thereof
EP3414322A4 (en) 2015-12-23 2020-03-04 Memorial Sloan-Kettering Cancer Center CELL THERAPY AND DRUG DISCOVERY BASED ON CELL LINES DERIVED FROM HUMAN ENTERAL NEURAL CREST DERIVED FROM PLURIPOTENT STEM CELLS
KR101733137B1 (ko) 2015-12-30 2017-05-08 (주)엑셀세라퓨틱스 연골조직 제조를 위한 3차원 오가노이드 블록 제작 방법
EP3397753B1 (en) 2015-12-30 2021-10-27 FUJIFILM Cellular Dynamics, Inc. Microtissue formation using stem cell-derived human hepatocytes
WO2017117571A1 (en) 2015-12-31 2017-07-06 President And Fellows Of Harvard College Neurons and compositions and methods for producing the same
WO2017117547A1 (en) 2015-12-31 2017-07-06 President And Fellows Of Harvard College Methods for generating neural tissue and uses thereof
US11021687B2 (en) 2016-01-08 2021-06-01 The Brigham And Women's Hospital, Inc. Production of differentiated enteroendocrine cells and insulin producing cells
EP3190176A1 (en) 2016-01-11 2017-07-12 IMBA-Institut für Molekulare Biotechnologie GmbH Method for tissue culture development on scaffold and differentiated tissue culture
HK1256807A1 (zh) 2016-01-14 2019-10-04 R·阿南德 神经类器官组合物及使用方法
US20170205396A1 (en) 2016-01-15 2017-07-20 Salk Institute For Biological Studies Systems and methods for culturing nephron progenitor cells
EP3411470A4 (en) 2016-02-01 2019-10-09 Emulate, Inc. SYSTEMS AND METHOD FOR BREEDING DARM CELLS IN MICROFLUIDIC DEVICES
JP7174408B2 (ja) 2016-02-10 2022-11-17 ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ 肝線維症のモデル系ならびにその作製および使用方法
WO2017139638A1 (en) 2016-02-11 2017-08-17 The Johns Hopkins University Compositions and methods for neuralgenesis
JP6947739B2 (ja) 2016-02-16 2021-10-13 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 既存のヒト中和抗体に対して耐性である新規組換えアデノ随伴ウイルスカプシド
WO2017142069A1 (ja) 2016-02-18 2017-08-24 学校法人慶應義塾 細胞培養培地、培養方法、及びオルガノイド
DK3417073T3 (da) 2016-02-19 2023-10-30 Procella Therapeutics Ab Genetiske markører til transplantation af humane, hjerteventrikulære progenitorceller
US20170267970A1 (en) 2016-02-29 2017-09-21 Whitehead Institute For Biomedical Research Three-Dimensional Hydrogels that Support Growth of Physiologically Relevant Tissue and Methods of Use Thereof
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
US20190086391A1 (en) 2016-03-08 2019-03-21 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method and system for continuous biosensing
WO2017160234A1 (en) 2016-03-14 2017-09-21 Agency For Science, Technology And Research Generation of midbrain-specific organoids from human pluripotent stem cells
WO2017160671A1 (en) 2016-03-15 2017-09-21 The Johns Hopkins University Enhanced organoid formation and intestinal stem cell renewal
US10772863B2 (en) 2016-03-15 2020-09-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Methods of inducing metabolic maturation of human pluripotent stem cells— derived hepatocytes
US20170285002A1 (en) 2016-03-16 2017-10-05 Public University Corporation Yokohama City University Method for reconstituting tumor with microenvironment
WO2017172638A1 (en) 2016-03-29 2017-10-05 Smsbiotech, Inc. Compositions and methods for using small mobile stem cells
WO2017176810A1 (en) 2016-04-04 2017-10-12 Biotime, Inc. Pluripotent stem cell-derived 3d retinal tissue and uses thereof
US20190128870A1 (en) 2016-04-04 2019-05-02 Humeltis Diagnostic Methods For Patient Specific Therapeutic Decision Making In Cancer Care
EP3228306A1 (en) 2016-04-04 2017-10-11 ratiopharm GmbH Complex compound comprising obeticholic acid and cyclodextrin and pharmaceutical formulation comprising the complex compound
JP6935101B2 (ja) 2016-04-05 2021-09-15 学校法人自治医科大学 幹細胞を再樹立する方法
EP3445385A4 (en) 2016-04-18 2019-11-20 The Trustees of Columbia University in the City of New York THERAPEUTIC GOALS INVOLVED IN THE PROGRESSION OF NON-ALCOHOLIC STEATOHEPATITIS (NASH)
CN117137917A (zh) 2016-04-22 2023-12-01 景凯生物科技股份有限公司 纳美芬、纳曲酮或其衍生物在治疗(非)酒精性脂肪肝炎或非酒精性脂肪性肝病的应用
US20170321188A1 (en) 2016-05-04 2017-11-09 The Research Foundation For The State University Of New York Methods of generating retinal progenitor cell preparations and uses thereof
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
US11760977B2 (en) 2016-05-25 2023-09-19 Salk Institute For Biological Studies Compositions and methods for organoid generation and disease modeling
US20170349659A1 (en) 2016-06-03 2017-12-07 The Board Of Trustees Of The Leland Stanford Junior University Wnt signaling agonist molecules
CA3027317A1 (en) 2016-06-14 2017-12-21 Cellply S.R.L. Screening kit and method
US12129491B2 (en) 2016-06-15 2024-10-29 Children's Medical Center Corporation Methods and compositions relating to lung cell differentiation
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
GB201610748D0 (en) 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method
GB201611982D0 (en) 2016-07-11 2016-08-24 Cellesce Ltd Cell culture
EP3275997A1 (en) 2016-07-28 2018-01-31 QGel SA Hydrogel precursor formulation and the use thereof
KR20190036553A (ko) 2016-08-02 2019-04-04 메모리얼 슬로안 케터링 캔서 센터 전이성 암의 치료 및 전이성 질환에 대한 모델 시스템
KR20250017294A (ko) 2016-08-03 2025-02-04 웨이크 포리스트 유니버시티 헬스 사이언시즈 암 모델링 플랫폼 및 그를 사용하는 방법
CA3032727A1 (en) 2016-08-04 2018-02-08 Wake Forest University Health Sciences Blood brain barrier model and methods of making and using the same
KR102417262B1 (ko) 2016-08-24 2022-07-07 각고호우징 게이오기주크 인간 설사병 바이러스의 감염·증식 배양용 2d 오가노이드 및 그 사용
JP7262385B2 (ja) 2016-08-26 2023-04-21 ザ・カウンシル・オブ・ザ・クィーンズランド・インスティテュート・オブ・メディカル・リサーチ 心筋細胞の成熟
EP3503902A4 (en) 2016-08-28 2020-04-22 Baylor College of Medicine A novel chicken egg-based metastasis model for cancer
US20210277102A1 (en) 2016-08-30 2021-09-09 Beth Israel Deaconess Medical Center, Inc. Compositions and methods for treating a tumor suppressor deficient cancer
WO2018044940A1 (en) 2016-08-30 2018-03-08 Beth Israel Deaconess Medical Center Compositions and methods for treating a tumor suppressor deficient cancer
EP4553082A3 (en) 2016-11-04 2025-08-20 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
JP7583524B2 (ja) 2016-11-23 2024-11-14 モルフォセル テクノロジーズ インコーポレイテッド 被包化肝組織
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
GB201622222D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
JP7248586B2 (ja) 2017-04-14 2023-03-29 チルドレンズ ホスピタル メディカル センター 複数ドナー幹細胞組成物およびそれを作製する方法
WO2018200481A1 (en) 2017-04-24 2018-11-01 Children's Hospital Medical Center Methods of making improved human intestinal organoid compositions via application of strain and human intestinal organoid compositions thereof
EP3395942A1 (en) 2017-04-25 2018-10-31 IMBA-Institut für Molekulare Biotechnologie GmbH Bi- or multi-differentiated organoid
WO2018226267A1 (en) 2017-06-09 2018-12-13 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
EP4678727A3 (en) 2018-07-26 2026-03-11 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CA3112026A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
EP3856204A4 (en) 2018-09-27 2022-06-29 Children's Hospital Medical Center Liver support system comprising liver organoids and methods of making and using same
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130137130A1 (en) * 2010-05-06 2013-05-30 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
WO2012168930A2 (en) * 2011-06-10 2012-12-13 Koninklijke Nederlandse Akademie Van Wetenschappen (Knaw) Culture media for stem cells

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KR20230110839A (ko) 2023-07-25
KR102807995B1 (ko) 2025-05-16
AU2017373767B2 (en) 2021-09-16

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