KR102421185B1 - 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 - Google Patents

생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Download PDF

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KR102421185B1
KR102421185B1 KR1020177003611A KR20177003611A KR102421185B1 KR 102421185 B1 KR102421185 B1 KR 102421185B1 KR 1020177003611 A KR1020177003611 A KR 1020177003611A KR 20177003611 A KR20177003611 A KR 20177003611A KR 102421185 B1 KR102421185 B1 KR 102421185B1
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dna
rna
membrane
capture surface
biological sample
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KR20170028432A (ko
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요한 칼 올로브 스코그
다니엘 엔덜
아파르나 라마찬드란
하오헹 얀
에밀리 버그호프
타이-펜 웨이
미켈 노어홀름
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엑소좀 디아그노스틱스, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tires In General (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020177003611A 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Active KR102421185B1 (ko)

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KR1020227023873A KR102596577B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US62/022,538 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US62/079,763 2014-11-14
US201562166890P 2015-05-27 2015-05-27
US62/166,890 2015-05-27
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

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KR1020227023873A Active KR102596577B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

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US (1) US10465183B2 (enExample)
EP (2) EP3623792B1 (enExample)
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CN (2) CN107002075B (enExample)
AU (2) AU2015287763B2 (enExample)
CA (2) CA3084920C (enExample)
ES (2) ES2762677T3 (enExample)
IL (2) IL250000B (enExample)
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WO (1) WO2016007755A1 (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2744589T3 (es) * 2011-05-11 2020-02-25 Exosome Diagnostics Inc Extracción de ácido nucleico de materiales biológicos heterogéneos
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
US10465183B2 (en) 2014-07-09 2019-11-05 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
CN105821031A (zh) * 2016-01-18 2016-08-03 昆明医科大学 一种二氯甲烷法提取dna的原理
US20190093172A1 (en) 2016-04-15 2019-03-28 Exosome Diagnostics, Inc. Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer
WO2017185086A1 (en) 2016-04-22 2017-10-26 Exosome Diagnostics, Inc. Devices and methods for in vivo capture of biological samples and nucleic acids therein
EP4299803A3 (en) 2016-05-02 2024-03-27 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
EP3452613B1 (en) 2016-05-05 2021-12-29 Exosome Diagnostics, Inc. Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection
CN109642229A (zh) * 2016-05-13 2019-04-16 外来体诊断公司 从生物液体分离细胞外囊泡和共分离无细胞dna的自动和手动方法
CA3033650A1 (en) 2016-08-17 2018-02-22 The Regents Of The University Of California A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay
KR102406951B1 (ko) 2016-09-14 2022-06-10 도레이 카부시키가이샤 무세포 dna의 회수 방법
US11396676B2 (en) 2016-10-21 2022-07-26 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
EP3548635B1 (en) 2016-11-30 2021-04-28 Exosome Diagnostics, Inc. Methods to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients
CN110072994A (zh) * 2016-12-23 2019-07-30 埃克波菲尔有限公司 用于纯化或分离微泡和外泌体的方法与组合物
US20200149036A1 (en) 2017-01-02 2020-05-14 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
US11830583B2 (en) * 2017-01-06 2023-11-28 Mantra Bio Inc. Systems and methods for characterizing extracellular vesicle (EV) population by predicting whether the EV originates from B, T, or dendritic cells
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
GB201706680D0 (en) * 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation
JP7225121B2 (ja) 2017-05-17 2023-02-20 エクソサム ダイアグノスティクス，インコーポレイティド 微小胞核酸および／またはタンパク質、並びに腎移植拒絶反応マーカーとしてのその使用
CN107267440A (zh) * 2017-06-09 2017-10-20 李刚 适用于外泌体提取的提取试剂及应用及其提取方法
EP3652315A4 (en) 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE
EP3652314B1 (en) * 2017-07-12 2023-08-23 Illumina, Inc. Nucleic acid extraction materials and methods
EP3655531B1 (en) * 2017-07-18 2024-09-04 Exosome Diagnostics, Inc. Sequencing of nucleic acids associated with exosomal isolation from patients with glioblastoma multiforme
KR102107844B1 (ko) * 2017-07-26 2020-05-07 ㈜로제타엑소좀 양이온을 이용한 세포밖 소포체의 분리 방법
EP3684383A4 (en) * 2017-09-20 2021-07-14 Molecular Stethoscope, Inc. METHODS AND SYSTEMS FOR DETECTION OF TISSUE DISORDERS
AU2018358057B2 (en) 2017-10-31 2023-03-02 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
US11260347B2 (en) * 2017-12-26 2022-03-01 Limited Liability Company “Prostagnost” Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration
US12429465B2 (en) 2018-02-20 2025-09-30 Korea University Research And Business Foundation Multi-column for isolating exosomes and exosome isolation method
CN112739832B (zh) 2018-06-06 2024-06-04 外来体诊断公司 用于开发尿液生物标志物和用于检测膀胱癌的方法
MX2020014024A (es) 2018-06-18 2021-03-09 Exopharm Ltd Metodos y composiciones para la purificacion o aislamiento de microvesiculas y exosomas.
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
US12038360B2 (en) * 2018-10-30 2024-07-16 Craif Inc. Device to be used for capturing extracellular vesicles, and preservation method and transport method for extracellular vesicles
WO2020106853A1 (en) 2018-11-20 2020-05-28 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
CN114072499B (zh) 2019-04-30 2024-08-06 Encodia公司 用于制备分析物的方法和相关试剂盒
CA3149690A1 (en) * 2019-08-08 2021-02-11 Biocartis Nv Novel nucleic acid purification chemistry
EP4031865A2 (en) 2019-09-18 2022-07-27 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
WO2021089606A1 (en) * 2019-11-04 2021-05-14 Nasasbiotech, S.L. Method for isolating nucleic acids
US20230009972A1 (en) * 2019-12-16 2023-01-12 Qiagen Gmbh Method for enriching vesicular rna
WO2021122846A1 (en) * 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
EP4141107B1 (en) * 2020-04-24 2026-03-11 Korea University Research and Business Foundation Microvesicle isolation method and microvesicle isolation device
US20230158483A1 (en) * 2020-04-24 2023-05-25 Toyobo Co., Ltd. Cellulose-based ion-exchange membrane, production method for same, exosome purification device, and exosome purification method
AU2021281287A1 (en) 2020-05-29 2023-01-19 Bio-Techne Corporation Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
WO2022133159A1 (en) 2020-12-17 2022-06-23 Nephrosant, Inc. Kits for stabilization of urine samples
US20240287498A1 (en) * 2021-06-17 2024-08-29 Qiagen Gmbh Method for isolating non-vesicular mirna
EP4370712A1 (en) 2021-07-16 2024-05-22 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
WO2023076528A2 (en) * 2021-10-28 2023-05-04 10X Genomics, Inc. Methods for sample preparation
AU2023221644A1 (en) 2022-02-18 2024-09-12 Bio-Techne Corporation Use of microvesicle signatures in the identification and treatment of renal disorders
EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
KR102784335B1 (ko) * 2023-02-09 2025-03-19 순천향대학교 산학협력단 세포유리핵산의 고농축화 분리 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036391A1 (en) * 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
US20140093880A1 (en) * 2012-09-28 2014-04-03 Samsung Electronics Co., Ltd. Compositions, kits, and methods for isolating vesicles

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
DE69433425T2 (de) * 1993-08-30 2004-10-07 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
WO2008045505A2 (en) * 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
ES2936256T3 (es) 2008-02-01 2023-03-15 Massachusetts Gen Hospital Uso de microvesículas en el diagnóstico, y pronóstico de enfermedades y afecciones médicas
WO2010065765A2 (en) 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
WO2010141862A2 (en) * 2009-06-05 2010-12-09 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
EP2576779B1 (en) 2010-06-01 2017-08-09 Qiagen GmbH Method for isolating and/or purifying nucleic acid(s)
EP2591359B1 (en) * 2010-07-07 2017-03-01 Aethlon Medical Inc Methods for quantifying exosomes
US8945509B2 (en) * 2010-08-20 2015-02-03 Life Technologies Corporation Magnetic beads having surface glycoconjugates and use thereof
EP2638057B1 (en) * 2010-11-10 2019-03-06 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
WO2012087241A1 (en) 2010-12-20 2012-06-28 Agency For Science, Technology And Research Method of purifying exosomes
ES2744589T3 (es) * 2011-05-11 2020-02-25 Exosome Diagnostics Inc Extracción de ácido nucleico de materiales biológicos heterogéneos
KR20140072014A (ko) * 2011-06-16 2014-06-12 카리스 라이프 사이언스 룩셈부르크 홀딩스, 에스.에이.알.엘. 생물지표 조성물 및 방법
KR20140005688A (ko) * 2012-07-06 2014-01-15 삼성전자주식회사 사용자 인터페이스 방법 및 장치
US20150353920A1 (en) 2013-01-03 2015-12-10 Exosome Diagnostics, Inc. Methods for isolating microvesicles
US10465183B2 (en) 2014-07-09 2019-11-05 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036391A1 (en) * 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
US20140093880A1 (en) * 2012-09-28 2014-04-03 Samsung Electronics Co., Ltd. Compositions, kits, and methods for isolating vesicles

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ES2898254T3 (es) 2022-03-04
AU2015287763B2 (en) 2019-08-08
EP3167062B1 (en) 2019-10-09
EP3167062A1 (en) 2017-05-17
JP6759182B2 (ja) 2020-09-23
SG11201700146QA (en) 2017-02-27
US10465183B2 (en) 2019-11-05
ES2762677T3 (es) 2020-05-25
CA2954576A1 (en) 2016-01-14
IL280307A (en) 2021-03-01
IL250000B (en) 2021-02-28
US20170198280A1 (en) 2017-07-13
IL250000A0 (en) 2017-03-30
EP3623792B1 (en) 2021-09-01
KR20220105172A (ko) 2022-07-26
EP3167062A4 (en) 2017-12-06
AU2015287763A1 (en) 2017-02-23
KR20170028432A (ko) 2017-03-13
CN113699143B (zh) 2024-08-06
CN113699143A (zh) 2021-11-26
CN107002075B (zh) 2021-07-30
IL280307B (en) 2022-02-01
JP2020202860A (ja) 2020-12-24
JP7128866B2 (ja) 2022-08-31
WO2016007755A1 (en) 2016-01-14
AU2019250221B2 (en) 2021-05-06
JP2017520264A (ja) 2017-07-27
CN107002075A (zh) 2017-08-01
KR102596577B1 (ko) 2023-10-30
CA2954576C (en) 2020-08-11
CA3084920C (en) 2023-02-28
EP3623792A1 (en) 2020-03-18
AU2019250221A1 (en) 2019-11-07
CA3084920A1 (en) 2016-01-14

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End annual number: 4