KR102421185B1 - 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 - Google Patents

생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Download PDF

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KR102421185B1
KR102421185B1 KR1020177003611A KR20177003611A KR102421185B1 KR 102421185 B1 KR102421185 B1 KR 102421185B1 KR 1020177003611 A KR1020177003611 A KR 1020177003611A KR 20177003611 A KR20177003611 A KR 20177003611A KR 102421185 B1 KR102421185 B1 KR 102421185B1
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dna
rna
membrane
capture surface
biological sample
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KR20170028432A (ko
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요한 칼 올로브 스코그
다니엘 엔덜
아파르나 라마찬드란
하오헹 얀
에밀리 버그호프
타이-펜 웨이
미켈 노어홀름
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엑소좀 디아그노스틱스, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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KR1020177003611A 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Active KR102421185B1 (ko)

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KR1020227023873A KR102596577B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US62/022,538 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US62/079,763 2014-11-14
US201562166890P 2015-05-27 2015-05-27
US62/166,890 2015-05-27
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

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KR1020227023873A Active KR102596577B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

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EP (2) EP3167062B1 (enExample)
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CN (2) CN113699143B (enExample)
AU (2) AU2015287763B2 (enExample)
CA (2) CA3084920C (enExample)
ES (2) ES2762677T3 (enExample)
IL (2) IL250000B (enExample)
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WO (1) WO2016007755A1 (enExample)

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EP3167062A4 (en) 2017-12-06
CA3084920C (en) 2023-02-28
KR20220105172A (ko) 2022-07-26
KR20170028432A (ko) 2017-03-13
CA2954576C (en) 2020-08-11
CN113699143B (zh) 2024-08-06
JP2017520264A (ja) 2017-07-27
SG11201700146QA (en) 2017-02-27
WO2016007755A1 (en) 2016-01-14
IL250000A0 (en) 2017-03-30
US10465183B2 (en) 2019-11-05
CA3084920A1 (en) 2016-01-14
KR102596577B1 (ko) 2023-10-30
AU2015287763A1 (en) 2017-02-23
CN107002075B (zh) 2021-07-30
JP6759182B2 (ja) 2020-09-23
ES2762677T3 (es) 2020-05-25
CN107002075A (zh) 2017-08-01
AU2019250221A1 (en) 2019-11-07
JP7128866B2 (ja) 2022-08-31
AU2015287763B2 (en) 2019-08-08
CN113699143A (zh) 2021-11-26
EP3623792B1 (en) 2021-09-01
US20170198280A1 (en) 2017-07-13
IL280307B (en) 2022-02-01
JP2020202860A (ja) 2020-12-24
AU2019250221B2 (en) 2021-05-06
ES2898254T3 (es) 2022-03-04
EP3167062A1 (en) 2017-05-17
IL280307A (en) 2021-03-01
IL250000B (en) 2021-02-28
EP3167062B1 (en) 2019-10-09
CA2954576A1 (en) 2016-01-14
EP3623792A1 (en) 2020-03-18

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