JP6759182B2 - 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 - Google Patents
微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 Download PDFInfo
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- JP6759182B2 JP6759182B2 JP2017501235A JP2017501235A JP6759182B2 JP 6759182 B2 JP6759182 B2 JP 6759182B2 JP 2017501235 A JP2017501235 A JP 2017501235A JP 2017501235 A JP2017501235 A JP 2017501235A JP 6759182 B2 JP6759182 B2 JP 6759182B2
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- dna
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Tires In General (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462022538P | 2014-07-09 | 2014-07-09 | |
| US62/022,538 | 2014-07-09 | ||
| US201462079763P | 2014-11-14 | 2014-11-14 | |
| US62/079,763 | 2014-11-14 | ||
| US201562166890P | 2015-05-27 | 2015-05-27 | |
| US62/166,890 | 2015-05-27 | ||
| PCT/US2015/039760 WO2016007755A1 (en) | 2014-07-09 | 2015-07-09 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020147390A Division JP7128866B2 (ja) | 2014-07-09 | 2020-09-02 | 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017520264A JP2017520264A (ja) | 2017-07-27 |
| JP2017520264A5 JP2017520264A5 (enExample) | 2018-08-16 |
| JP6759182B2 true JP6759182B2 (ja) | 2020-09-23 |
Family
ID=55064901
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017501235A Active JP6759182B2 (ja) | 2014-07-09 | 2015-07-09 | 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 |
| JP2020147390A Active JP7128866B2 (ja) | 2014-07-09 | 2020-09-02 | 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020147390A Active JP7128866B2 (ja) | 2014-07-09 | 2020-09-02 | 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10465183B2 (enExample) |
| EP (2) | EP3623792B1 (enExample) |
| JP (2) | JP6759182B2 (enExample) |
| KR (2) | KR102596577B1 (enExample) |
| CN (2) | CN107002075B (enExample) |
| AU (2) | AU2015287763B2 (enExample) |
| CA (2) | CA3084920C (enExample) |
| ES (2) | ES2762677T3 (enExample) |
| IL (2) | IL250000B (enExample) |
| SG (1) | SG11201700146QA (enExample) |
| WO (1) | WO2016007755A1 (enExample) |
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| CA2835641C (en) * | 2011-05-11 | 2019-05-28 | Exosome Diagnostics, Inc. | Nucleic acid extraction from heterogeneous biological materials |
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| SG11201700146QA (en) | 2014-07-09 | 2017-02-27 | Exosome Diagnostics Inc | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN105821031A (zh) * | 2016-01-18 | 2016-08-03 | 昆明医科大学 | 一种二氯甲烷法提取dna的原理 |
| JP2019513391A (ja) | 2016-04-15 | 2019-05-30 | エクソサム ダイアグノスティクス，インコーポレイティド | 未分化リンパ腫キナーゼ（ａｌｋ）核酸およびａｌｋ融合転写産物の血漿ベースの検出、並びに癌の診断と治療におけるそれらの使用 |
| WO2017185086A1 (en) | 2016-04-22 | 2017-10-26 | Exosome Diagnostics, Inc. | Devices and methods for in vivo capture of biological samples and nucleic acids therein |
| KR102516168B1 (ko) | 2016-05-02 | 2023-03-30 | 엔코디아, 인코포레이티드 | 암호화 핵산을 사용한 거대분자 분석 |
| ES2908932T3 (es) | 2016-05-05 | 2022-05-04 | Exosome Diagnostics Inc | Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal |
| JP7037504B2 (ja) * | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| EP3845665A1 (en) | 2016-08-17 | 2021-07-07 | The Regents Of The University Of California | A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay |
| WO2018052011A1 (ja) | 2016-09-14 | 2018-03-22 | 東レ株式会社 | セルフリーｄｎａの回収方法 |
| US11396676B2 (en) | 2016-10-21 | 2022-07-26 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| WO2018102162A1 (en) | 2016-11-30 | 2018-06-07 | Exosome Diagnostics, Inc. | Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients |
| KR20190100174A (ko) * | 2016-12-23 | 2019-08-28 | 엑소팜 리미티드 | 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물 |
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| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
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| CN107267440A (zh) * | 2017-06-09 | 2017-10-20 | 李刚 | 适用于外泌体提取的提取试剂及应用及其提取方法 |
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| EP3652315A4 (en) | 2017-07-12 | 2021-09-01 | Exosome Diagnostics, Inc. | METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE |
| US11345957B2 (en) | 2017-07-18 | 2022-05-31 | Exosome Diagnostics, Inc. | Methods of treating glioblastoma in a subject informed by exosomal RNA signatures |
| JP2020528766A (ja) * | 2017-07-26 | 2020-10-01 | ロゼッタ エクソソーム | 陽イオンを利用した細胞外小胞体の分離方法 |
| SG11202003588QA (en) * | 2017-09-20 | 2020-05-28 | Molecular Stethoscope Inc | Methods and systems for detecting tissue conditions |
| MX2020004559A (es) | 2017-10-31 | 2020-10-05 | Encodia Inc | Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico. |
| WO2019132688A1 (ru) * | 2017-12-26 | 2019-07-04 | Общество С Ограниченной Ответственностью "Простагност" | Способ и устройство для выделения внеклеточных везикул из биологических жидкостей с помощью каскадной ультрафильтрации |
| CN111770931B (zh) * | 2018-02-20 | 2023-08-25 | 高丽大学校产学协力团 | 用于分离外泌体的多柱及外泌体分离方法 |
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| CN112585446A (zh) | 2018-06-18 | 2021-03-30 | 埃克波菲尔有限公司 | 用于纯化或分离微泡和外泌体的方法与组合物 |
| CN108841777A (zh) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | 基于静电吸附的胞外囊泡及其内含物的提取方法及装置 |
| JP7426725B2 (ja) * | 2018-10-30 | 2024-02-02 | Ｃｒａｉｆ株式会社 | 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法 |
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| KR102784335B1 (ko) * | 2023-02-09 | 2025-03-19 | 순천향대학교 산학협력단 | 세포유리핵산의 고농축화 분리 방법 |
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| KR20140005688A (ko) * | 2012-07-06 | 2014-01-15 | 삼성전자주식회사 | 사용자 인터페이스 방법 및 장치 |
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-
2015
- 2015-07-09 SG SG11201700146QA patent/SG11201700146QA/en unknown
- 2015-07-09 CA CA3084920A patent/CA3084920C/en active Active
- 2015-07-09 JP JP2017501235A patent/JP6759182B2/ja active Active
- 2015-07-09 EP EP19201765.5A patent/EP3623792B1/en active Active
- 2015-07-09 WO PCT/US2015/039760 patent/WO2016007755A1/en not_active Ceased
- 2015-07-09 ES ES15818561T patent/ES2762677T3/es active Active
- 2015-07-09 CA CA2954576A patent/CA2954576C/en active Active
- 2015-07-09 ES ES19201765T patent/ES2898254T3/es active Active
- 2015-07-09 KR KR1020227023873A patent/KR102596577B1/ko active Active
- 2015-07-09 CN CN201580049375.XA patent/CN107002075B/zh active Active
- 2015-07-09 KR KR1020177003611A patent/KR102421185B1/ko active Active
- 2015-07-09 AU AU2015287763A patent/AU2015287763B2/en active Active
- 2015-07-09 CN CN202110781733.3A patent/CN113699143B/zh active Active
- 2015-07-09 US US15/325,021 patent/US10465183B2/en active Active
- 2015-07-09 EP EP15818561.1A patent/EP3167062B1/en active Active
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2017
- 2017-01-09 IL IL250000A patent/IL250000B/en active IP Right Grant
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2019
- 2019-10-17 AU AU2019250221A patent/AU2019250221B2/en active Active
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2020
- 2020-09-02 JP JP2020147390A patent/JP7128866B2/ja active Active
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2021
- 2021-01-20 IL IL280307A patent/IL280307B/en unknown
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| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |