JP6759182B2 - 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 - Google Patents

微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 Download PDF

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JP6759182B2
JP6759182B2 JP2017501235A JP2017501235A JP6759182B2 JP 6759182 B2 JP6759182 B2 JP 6759182B2 JP 2017501235 A JP2017501235 A JP 2017501235A JP 2017501235 A JP2017501235 A JP 2017501235A JP 6759182 B2 JP6759182 B2 JP 6759182B2
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rna
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JP2017520264A (ja
JP2017520264A5 (enExample
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カール オラブ スコグ ジョアン
カール オラブ スコグ ジョアン
エンダーレ ダニエル
エンダーレ ダニエル
ラマチャンドラン アパルナ
ラマチャンドラン アパルナ
イエン ハオホゥオン
イエン ハオホゥオン
バーグホフ エミリー
バーグホフ エミリー
ウエイ タイ−フェン
ウエイ タイ−フェン
ノアーホルム ミッケル
ノアーホルム ミッケル
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エクソサム ダイアグノスティクス，インコーポレイティド
エクソサム ダイアグノスティクス，インコーポレイティド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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JP2017501235A 2014-07-09 2015-07-09 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法 Active JP6759182B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US62/022,538 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US62/079,763 2014-11-14
US201562166890P 2015-05-27 2015-05-27
US62/166,890 2015-05-27
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

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JP2017520264A JP2017520264A (ja) 2017-07-27
JP2017520264A5 JP2017520264A5 (enExample) 2018-08-16
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JP2020147390A Active JP7128866B2 (ja) 2014-07-09 2020-09-02 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法

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US (1) US10465183B2 (enExample)
EP (2) EP3623792B1 (enExample)
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KR (2) KR102421185B1 (enExample)
CN (2) CN113699143B (enExample)
AU (2) AU2015287763B2 (enExample)
CA (2) CA2954576C (enExample)
ES (2) ES2762677T3 (enExample)
IL (2) IL250000B (enExample)
SG (1) SG11201700146QA (enExample)
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Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012155014A1 (en) * 2011-05-11 2012-11-15 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
SG11201700146QA (en) 2014-07-09 2017-02-27 Exosome Diagnostics Inc Methods for isolating microvesicles and extracting nucleic acids from biological samples
CN105821031A (zh) * 2016-01-18 2016-08-03 昆明医科大学 一种二氯甲烷法提取dna的原理
SG11201809005TA (en) 2016-04-15 2018-11-29 Exosome Diagnostics Inc Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer
WO2017185086A1 (en) 2016-04-22 2017-10-26 Exosome Diagnostics, Inc. Devices and methods for in vivo capture of biological samples and nucleic acids therein
CA3022863A1 (en) 2016-05-02 2017-11-09 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding of molecular recognition events
EP3452613B1 (en) 2016-05-05 2021-12-29 Exosome Diagnostics, Inc. Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection
AU2017263886B2 (en) * 2016-05-13 2023-11-09 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
EP3845665A1 (en) 2016-08-17 2021-07-07 The Regents Of The University Of California A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay
US20190203200A1 (en) 2016-09-14 2019-07-04 Toray Industries, Inc. Method of collecting cell-free dna
EP3529374B1 (en) 2016-10-21 2024-04-03 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
CN110446790B (zh) 2016-11-30 2023-03-31 外来体诊断公司 使用外来体rna和无细胞dna检测血浆中的突变的方法和组合物
JP7376091B2 (ja) * 2016-12-23 2023-11-08 エクソファーマ リミテッド 微小胞およびエキソソームの精製または単離のための方法および組成物
WO2018126278A2 (en) 2017-01-02 2018-07-05 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
US11830583B2 (en) * 2017-01-06 2023-11-28 Mantra Bio Inc. Systems and methods for characterizing extracellular vesicle (EV) population by predicting whether the EV originates from B, T, or dendritic cells
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
GB201706680D0 (en) * 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation
WO2018213392A1 (en) 2017-05-17 2018-11-22 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
CN107267440A (zh) * 2017-06-09 2017-10-20 李刚 适用于外泌体提取的提取试剂及应用及其提取方法
WO2019013991A2 (en) 2017-07-12 2019-01-17 Illumina, Inc. METHODS, SYSTEMS AND MATERIALS FOR EXTRACTING NUCLEIC ACIDS
CN111133106A (zh) 2017-07-12 2020-05-08 外来体诊断公司 用于分离和富集生物流体来源的细胞外囊泡的方法及其使用方法
EP3655531B1 (en) * 2017-07-18 2024-09-04 Exosome Diagnostics, Inc. Sequencing of nucleic acids associated with exosomal isolation from patients with glioblastoma multiforme
JP2020528766A (ja) * 2017-07-26 2020-10-01 ロゼッタ エクソソーム 陽イオンを利用した細胞外小胞体の分離方法
SG11202003588QA (en) * 2017-09-20 2020-05-28 Molecular Stethoscope Inc Methods and systems for detecting tissue conditions
JP7390027B2 (ja) 2017-10-31 2023-12-01 エンコディア， インコーポレイテッド 核酸エンコーディングおよび／または標識を使用する解析のためのキット
US11260347B2 (en) * 2017-12-26 2022-03-01 Limited Liability Company “Prostagnost” Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration
CN111770931B (zh) * 2018-02-20 2023-08-25 高丽大学校产学协力团 用于分离外泌体的多柱及外泌体分离方法
US12378608B2 (en) 2018-06-06 2025-08-05 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
CA3104349A1 (en) 2018-06-18 2019-12-26 Exopharm Limited Methods and compositions for purification or isolation of microvesicles and exosomes
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
EP3875570A4 (en) * 2018-10-30 2022-08-24 Craif Inc. DEVICE USED FOR CONTAINING EXTRACELLULAR VESICLES AND METHODS FOR PRESERVING AND TRANSPORTING EXTRACELLULAR VESICLES
EP3884045B1 (en) 2018-11-20 2023-01-11 Exosome Diagnostics, Inc. Methods for internal controls of microvesicle isolations
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
WO2021023854A1 (en) * 2019-08-08 2021-02-11 Biocartis Nv Novel nucleic acid purification chemistry
WO2021055770A2 (en) 2019-09-18 2021-03-25 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
JP2023501354A (ja) * 2019-11-04 2023-01-18 ナサスビオテック、ソシエダッド、リミターダ 核酸の単離方法
EP4077659A1 (en) * 2019-12-16 2022-10-26 QIAGEN GmbH Enrichment method
EP4077660A1 (en) * 2019-12-16 2022-10-26 QIAGEN GmbH Method for enriching vesicular rna
EP4140568A4 (en) * 2020-04-24 2024-05-22 Toyobo Co., Ltd. Ion exchange membrane
CN115698265A (zh) * 2020-04-24 2023-02-03 高丽大学校算学协力团 微囊泡分离方法及微囊泡分离装置
CA3180572A1 (en) 2020-05-29 2021-12-02 Johan Skog Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
WO2022133159A1 (en) 2020-12-17 2022-06-23 Nephrosant, Inc. Kits for stabilization of urine samples
WO2022263500A1 (en) * 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna
US20250075258A1 (en) 2021-07-16 2025-03-06 Exosome Diagnostics, Inc. Methods of detecting sjogren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
WO2023076528A2 (en) * 2021-10-28 2023-05-04 10X Genomics, Inc. Methods for sample preparation
US20250163510A1 (en) 2022-02-18 2025-05-22 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
KR102784335B1 (ko) * 2023-02-09 2025-03-19 순천향대학교 산학협력단 세포유리핵산의 고농축화 분리 방법

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
ATE256735T1 (de) * 1993-08-30 2004-01-15 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
FR2788780B1 (fr) * 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
ES2588227T3 (es) * 2006-10-10 2016-10-31 Trovagene, Inc. Composiciones, métodos y kits para aislar ácidos nucleicos de fluidos corporales usando medios de intercambio aniónico
US20110053157A1 (en) 2008-02-01 2011-03-03 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2010065765A2 (en) * 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
WO2010141862A2 (en) * 2009-06-05 2010-12-09 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
EP2576779B1 (en) 2010-06-01 2017-08-09 Qiagen GmbH Method for isolating and/or purifying nucleic acid(s)
ES2624284T3 (es) * 2010-07-07 2017-07-13 Aethlon Medical Inc Métodos para cuantificar exosomas
US8945509B2 (en) * 2010-08-20 2015-02-03 Life Technologies Corporation Magnetic beads having surface glycoconjugates and use thereof
US20130295574A1 (en) * 2010-11-10 2013-11-07 Exosome Diagnostics, Inc. Method for Isolation of Nucleic Acid Containing Particles and Extraction of Nucleic Acids Therefrom
SG190450A1 (en) * 2010-12-20 2013-06-28 Agency Science Tech & Res Method of purifying exosomes
WO2012155014A1 (en) * 2011-05-11 2012-11-15 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
BR112013032232A2 (pt) * 2011-06-16 2016-09-20 Caris Life Sciences Switzerland Holdings S A R L método de caracterização de câncer através do uso de biomarcador de ácido nucleico
KR20140005688A (ko) * 2012-07-06 2014-01-15 삼성전자주식회사 사용자 인터페이스 방법 및 장치
CN104995311A (zh) * 2012-08-30 2015-10-21 外来体诊断公司 用于核酸测定的对照
KR101933621B1 (ko) * 2012-09-28 2018-12-28 삼성전자주식회사 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법
EP3499212B1 (en) 2013-01-03 2020-08-12 Exosome Diagnostics, Inc. Methods for isolating microvesicles
SG11201700146QA (en) 2014-07-09 2017-02-27 Exosome Diagnostics Inc Methods for isolating microvesicles and extracting nucleic acids from biological samples

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IL280307B (en) 2022-02-01
EP3167062A4 (en) 2017-12-06
CA3084920C (en) 2023-02-28
CN113699143B (zh) 2024-08-06
EP3623792B1 (en) 2021-09-01
AU2015287763A1 (en) 2017-02-23
KR102421185B1 (ko) 2022-07-14
US10465183B2 (en) 2019-11-05
IL250000A0 (en) 2017-03-30
KR20220105172A (ko) 2022-07-26
JP2017520264A (ja) 2017-07-27
WO2016007755A1 (en) 2016-01-14
US20170198280A1 (en) 2017-07-13
JP7128866B2 (ja) 2022-08-31
CN113699143A (zh) 2021-11-26
IL280307A (en) 2021-03-01
AU2015287763B2 (en) 2019-08-08
CA2954576C (en) 2020-08-11
EP3167062A1 (en) 2017-05-17
EP3623792A1 (en) 2020-03-18
SG11201700146QA (en) 2017-02-27
KR20170028432A (ko) 2017-03-13
IL250000B (en) 2021-02-28
AU2019250221B2 (en) 2021-05-06
CN107002075A (zh) 2017-08-01
EP3167062B1 (en) 2019-10-09
KR102596577B1 (ko) 2023-10-30
JP2020202860A (ja) 2020-12-24
AU2019250221A1 (en) 2019-11-07
CA3084920A1 (en) 2016-01-14
CN107002075B (zh) 2021-07-30
ES2898254T3 (es) 2022-03-04
ES2762677T3 (es) 2020-05-25
CA2954576A1 (en) 2016-01-14

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